Abstract:
:One type of covalent linkages connecting lignin and hemicellulose in plant cell walls is the ester linkage between 4-O-methyl-D-glucuronic acid of glucuronoxylan and lignin alcohols. An enzyme that could hydrolyze such linkages, named glucuronoyl esterase, occurs in the cellulolytic system of the wood-rotting fungus Schizophyllum commune. Here we report partial amino acid sequences of the enzyme and the results of subsequent search for homologous genes in sequenced genomes. The homologous genes of unknown functions were found in genomes of several filamentous fungi and one bacterium. The gene corresponding to the cip2 gene of Hypocrea jecorina (Trichoderma reesei), known to be up-regulated under conditions of induction of cellulolytic and hemicellulolytic enzymes, was over-expressed in H. jecorina. The product of the cip2 gene was purified to homogeneity and shown to exhibit glucuronoyl esterase activity.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Li XL,Spániková S,de Vries RP,Biely Pdoi
10.1016/j.febslet.2007.07.041subject
Has Abstractpub_date
2007-08-21 00:00:00pages
4029-35issue
21eissn
0014-5793issn
1873-3468pii
S0014-5793(07)00814-9journal_volume
581pub_type
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