Abstract:
:Phlorizin, a potent inhibitor of the Na+/D-glucose cotransporter, was derivatised to 3-aminophlorizin and subsequently coupled to Affi-Gel 15. Affinity chromatography of pig kidney brush border membranes solubilised in Triton X-100 allowed the purification of a 60 kDa protein on this resin. We consider this protein to be the Na+/D-glucose cotransporter, or part of it, for the following reasons: (i) binding of this protein to Affi-Gel 15 specifically requires phlorizin covalently attached to the resin and is lowered when phlorizin is replaced by phloretin; (ii) binding of the 60 kDa protein to a phlorizin affinity column requires the presence of Na+; (iii) polyclonal as well as monoclonal antibodies against the 60 kDa protein inhibit binding of phlorizin to brush border membranes from rabbit and pig kidney.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Kitlar T,Morrison AI,Kinne R,Deutscher Jdoi
10.1016/0014-5793(88)81315-2subject
Has Abstractpub_date
1988-07-04 00:00:00pages
115-9issue
1eissn
0014-5793issn
1873-3468pii
0014-5793(88)81315-2journal_volume
234pub_type
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