Abstract:
:A method was established for measuring molecule numbers of three different myosin heavy chain (MHC) mRNA isoforms in total RNA preparations. The quantification was based on a combination of primer-directed reverse transcriptase and polymerase chain reactions with 5'-digoxigenin-labeled oligonucleotides, using external standards. The sensitivity of the method allowed the quantitation of mRNA amounts down to the range of 1,000 molecules (detection limit 50 molecules). The numbers determined for eight different rabbit muscles are in the range of 10(3)-10(9)/micrograms total RNA. In soleus muscle, the value of 1.11 x 10(9) MHCI mRNA molecules corresponds to approximately 8% of the total mRNA. With reference to myonuclei, this amount corresponds to 1-2 x 10(4) molecules/nucleus. A quantitative comparison of the two fast MHC mRNA isoforms with the distribution of different MHC isoforms at the protein level indicates that one of these two fast sequences is specific to MHCIIb and the other to MHCIId. However, our data point to the existence of additional MHCIId mRNA subtypes.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Peuker H,Pette Ddoi
10.1016/0014-5793(93)80523-wsubject
Has Abstractpub_date
1993-03-08 00:00:00pages
253-8issue
3eissn
0014-5793issn
1873-3468pii
0014-5793(93)80523-Wjournal_volume
318pub_type
杂志文章相关文献
FEBS LETTERS文献大全abstract::Lysophospholipids are liberated during venomous action. In this study we demonstrated that lysophosphatidyl choline (LPC) of various acyl chains enhances considerably the activity of snake venom phosphodiesterase (PDE). Lysophosphatidic acid (LPA) and its cyclic form (cLPA), on the other hand, were found to inhibit th...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)01142-9
更新日期:1998-10-02 00:00:00
abstract::The structure of the complex of Ras with the Ras-binding domain of its effector RalGDS (RGS-RBD), the first genuine Ras-effector complex, has been solved by X-ray crystallography. As with the Rap-RafRBD complex (Nasser et al., 1995), the interaction is via an inter-protein beta-sheet between the switch I region of Ras...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)00555-4
更新日期:1999-05-21 00:00:00
abstract::Constitutively active tyrosine kinases are frequently expressed in various types of human leukemias as the result of chromosomal translocations. The TEL-Jak2 fusion oncoprotein possesses transforming properties in both animal and cellular models, that are tightly dependent on Stat5 activation. In the IL-3-independent ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(01)02452-8
更新日期:2001-05-25 00:00:00
abstract::While many antimicrobial peptides (AMPs) disrupt bacterial membranes, some translocate into bacteria and interfere with intracellular processes. Buforin II and DesHDAP1 are thought to kill bacteria by interacting with nucleic acids. Here, molecular modeling and experimental measurements are used to show that neither n...
journal_title:FEBS letters
pub_type: 信件
doi:10.1002/1873-3468.12574
更新日期:2017-03-01 00:00:00
abstract::Very long-chain fatty acids (VLCFAs) have a variety of physiological functions and are related to numerous disorders. The key step of VLCFA elongation is catalyzed by members of the elongase family, ELOVLs. Mammals have seven ELOVLs (ELOVL1-7), yet none of them has been purified and analyzed. In the presented study we...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2011.09.024
更新日期:2011-10-20 00:00:00
abstract::The possible relationship between pyridoxal phosphate-dependent ornithine decarboxylase (ODC) activity and glucose-6-phosphate dehydrogenase (G6PD) activity has been studied in the osteoblasts of the growth-plate of metatarsals of rats fed a pyridoxine-deficient diet, which caused depressed G6PD levels. The G6PD activ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(86)80579-8
更新日期:1986-05-26 00:00:00
abstract::α-Glucosidase hydrolyzes α-glucosides and transfers α-glucosyl residues to an acceptor through transglucosylation. In this study, GH13_31 α-glucosidase BspAG13_31A with high transglucosylation activity is reported in Bacillus sp. AHU2216 and biochemically and structurally characterized. This enzyme is specific to α-(1...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1002/1873-3468.13126
更新日期:2018-07-01 00:00:00
abstract::Terminal oligopyrimidine (TOP) mRNAs are a group of messengers translationally regulated according to the growth status of the cell. Two hypotheses have been proposed for the mechanism of the regulation: (i) there is a specific translational regulator which can reversibly alter TOP-mRNA structure, (ii) a component of ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)01209-x
更新日期:1997-10-27 00:00:00
abstract::Structure/function relationships in troponin C are studied with vertebrate fast-twitch fibers by exchanging the skeletal troponin C with two bacterially synthesized recombinant proteins designed by site-directed mutagenesis of cardiac troponin C. One mutant (CBM1) contained an additional active site, by deleting Val-2...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(89)80420-x
更新日期:1989-05-08 00:00:00
abstract::Human nuclear cyclophilin 33 (hCyP33) was the first protein which was found to contain an RNA-binding motif and a PPIase domain. It was not known what cellular and physiological roles are played by the RNA-binding activity as well as the PPIase activity of hCyP33. In this paper, we investigated the binding specificity...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2008.01.055
更新日期:2008-03-05 00:00:00
abstract::Rat brain microtubules were prepared at the adult stage and from immature (i.e., 4-day-old) animals. At an early stage of development, the composition of microtubule-associated proteins is qualitatively different from that found at the adult stage [(1982) Eur. J. Biochem. 129, 465-471]. The influence of calmodulin on ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(84)81148-5
更新日期:1984-07-09 00:00:00
abstract::Highly selective affinity labeling of the primer site of E. coli DNA polymerase I was performed with the 5'-reactive derivatives of oligothymidylate in the presence of poly(dA) template. Subtilysine cleavage proved that the site of affinity modification belonged to the 'Klenow' part of DNA polymerase I. If taken separ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(90)80478-2
更新日期:1990-10-15 00:00:00
abstract::We have purified a form of protein phosphatase 1 (PP1) from HeLa cell nuclei, in which the phosphatase is complexed to a regulatory subunit termed p99. We report here the cloning and characterisation of the p99 component. p99 mRNA is widely expressed in human tissues and immunofluorescence analysis with anti-p99 antib...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)01485-3
更新日期:1997-12-22 00:00:00
abstract::Okadaic acid is an inhibitor of the protein Ser/Thr phosphatases PP1 and PP2A, which blocks the activation of extracellular signal-regulated protein kinase 5 (ERK5), a member of the MAP kinase family activated by growth factors and several types of stressors. The blocking of ERK5 activation by okadaic acid was observe...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(02)02950-2
更新日期:2002-07-17 00:00:00
abstract::The sarcoplasmic reticulum-bound creatine kinase from rabbit skeletal muscle was inhibited by the nitric oxide donor S-nitrosoglutathione (GSNO). This led to a decrease in Ca2+ uptake in sarcoplasmic reticulum vesicles when the transport was driven by ATP generated from phosphocreatine and ADP. In contrast, the Ca 2+ ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(96)00829-0
更新日期:1996-09-02 00:00:00
abstract::Chronic neuroleptic treatment induces an increase in the density of D2 dopamine receptors in the striatum. The effect of prolonged administration of haloperidol on mRNA levels of the short (D2S) and long (D2L) isoforms of the D2 dopamine receptor and the D3 dopamine receptor in different brain regions was examined. Ha...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)80385-4
更新日期:1994-02-14 00:00:00
abstract::Mutation of Thr90 to Ala has a profound effect on bacteriorhodopsin properties. T90A shows about 20% of the proton pumping efficiency of wild type, once reconstituted into liposomes. Mutation of Thr90 influences greatly the Schiff base/Asp85 environment, as demonstrated by altered lambda(max) of 555 nm and pK(a) of As...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(01)03080-0
更新日期:2001-11-23 00:00:00
abstract::Rat calvaria cells grown in culture for one week had properties of osteoblasts: a high content in alkaline phosphatase and a marked cyclic AMP response to parathyroid hormone (PTH). In short-term experiments, insulin-like growth factor I (IGF I) stimulated the incorporation of [14C] glucose into glycogen. When IGF I w...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(84)81015-7
更新日期:1984-07-23 00:00:00
abstract::Extracellular vesicles (EVs) contain microRNAs (miRNAs). However, the exact molecular mechanisms of the recruitment of miRNAs in EVs are not well characterized. Based on proteomic analysis, we identified that silencing of Annexin A2 (ANXA2) significantly decreased the amount of miRNAs in EVs. In addition, microarray a...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2015.11.036
更新日期:2015-12-21 00:00:00
abstract::Cytosolic NADP(+)-dependent malic enzyme (ME) from human tumor cells was characterized in detail and compared to ME from normal human tissues produced in recombinant E. coli. Kinetic properties, size as seen in SDS gels, and HPLC elution profiles of tryptic digests of human 'normal cell' ME and NADP(+)-ME from tumor c...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)00386-6
更新日期:1994-05-16 00:00:00
abstract::The herpesvirus life cycle has two distinct phases: latency and lytic replication. The viral immediate early protein replication and transcription activator (RTA) plays a central role in mediating the balance between these two phases. Here, we demonstrate that a B cell terminal differentiation factor X-box binding pro...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2007.06.056
更新日期:2007-07-24 00:00:00
abstract::Plant receptor-like kinases (RLKs) share their evolutionary origin with animal interleukin-1 receptor-associated kinase (IRAK)/Pelle family of soluble kinases and are distinguished by having tyrosine as 'gatekeeper'. This position is adjacent to the hinge region and is hidden in a hydrophobic pocket of the catalytic c...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2013.07.050
更新日期:2013-09-17 00:00:00
abstract::Hterologous complementation of yeast mutants has enabled the isolation of genes encoding several families of amino acid transporters. Among them, NTR1 codes for a membrane protein with weak histidine transport activity. However, at the sequence level, NTR1 is related to rather non-specific oligopeptide transporters fr...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(95)00853-2
更新日期:1995-08-21 00:00:00
abstract::MDNCF is a human monocyte-derived, 72-residue chemotactic peptide, which has sequence similarity with members of a family of pro-inflammatory cytokines. The peptide was synthesized by the solid-phase method, and is identical to the natural peptide in amino acid composition, sequence and chemotactic potency. MDNCF form...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(88)80078-4
更新日期:1988-08-29 00:00:00
abstract::Accumulation of hyperphosphorylated Tau is associated with a number of neurodegenerative diseases collectively known as tauopathies. Differences in clinical and cognitive profiles among them suggest differential sensitivity of neuronal populations to Tau levels, phosphorylation and mutations. We used tissue specific e...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2006.07.045
更新日期:2006-08-21 00:00:00
abstract::Novel catechin derivatives obtained from grape procyanidins and l-cysteine scavenge free radicals by hydrogen atom donation, rather than electron transfer, and reduce cell viability in A375 and M21 melanoma cells. In particular, 4beta-(S-cysteinyl)epicatechin 3-O-gallate has a free radical scavenging capacity as stron...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2005.06.051
更新日期:2005-08-15 00:00:00
abstract::Recently, it has been shown that really interesting new gene (RING)-in between ring finger (IBR)-RING domain-containing proteins, such as Parkin and Parc, are E3 ubiquitin ligases and are involved in regulation of apoptosis. In this report, we show that p53-inducible RING-finger protein (p53RFP), a p53-inducible E3 ub...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2005.09.105
更新日期:2006-02-06 00:00:00
abstract::Cerebral deposits of beta/A4 amyloid protein is a pathologic sign of Alzheimer's disease. A synthetic partial-length (1-28) peptide of this protein contains one glutamine and two lysine residues. Here we show that this peptide can be a substrate of transglutaminase, which catalyzes cross-linking between glutamine and ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)81772-r
更新日期:1993-07-12 00:00:00
abstract::We examined whether a proposed spatial proximity between Asp114(2.50) and Asn332(7.49) affected the functional properties of the mu opioid receptor. The D114(2.50)N mutant had reduced binding affinities for morphine, DAMGO and CTAP, but not for naloxone and [3H]diprenorphine; this mutation also abolished agonist-induc...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)00316-6
更新日期:1999-03-26 00:00:00
abstract::The yeast integral plasma membrane protein Ist2 belongs to a group of membrane proteins which are synthesized from localized mRNAs. The protein reaches the plasma membrane via the ER on a route operating independently of the classical secretory pathway. We have identified a complex peptide-sorting signal located at th...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2006.12.048
更新日期:2007-02-06 00:00:00