Abstract:
:While many antimicrobial peptides (AMPs) disrupt bacterial membranes, some translocate into bacteria and interfere with intracellular processes. Buforin II and DesHDAP1 are thought to kill bacteria by interacting with nucleic acids. Here, molecular modeling and experimental measurements are used to show that neither nucleic acid binding peptide selectively binds DNA sequences. Simulations and experiments also show that changing lysines to arginines enhances DNA binding, suggesting that including additional guanidinium groups is a potential strategy to engineer more potent AMPs. Moreover, the lack of binding specificity may make it more difficult for bacteria to evolve resistance to these and other similar AMPs.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Sim S,Wang P,Beyer BN,Cutrona KJ,Radhakrishnan ML,Elmore DEdoi
10.1002/1873-3468.12574subject
Has Abstractpub_date
2017-03-01 00:00:00pages
706-717issue
5eissn
0014-5793issn
1873-3468journal_volume
591pub_type
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