Human CyP33 binds specifically to mRNA and binding stimulates PPIase activity of hCyP33.

abstract：:The relationship between different amino acids at the beta 6 position of hemoglobin and tetramer stability was addressed by a site-directed mutagenesis approach. Precipitation rates during mechanical agitation of oxyhemoglobins with Gln, Ala, Val, Leu and Trp at the beta 6 position increased 2, 5, 13, 21 and 53 times,...

journal_title：FEBS letters

authors： Adachi K,Kim JY,Konitzer P,Asakura T,Saviola B,Surrey S

更新日期：1993-01-02 00:00:00

abstract：:Vibrio vulnificus is an opportunistic human pathogen that causes severe infections in susceptible individuals. While the components of the Escherichia coli phosphoenolpyruvate: sugar phosphotransferase system (PTS) have been shown to regulate numerous targets, little such information is available for the V. vulnificus...

journal_title：FEBS letters

authors： Kim YJ,Ryu Y,Koo BM,Lee NY,Chun SJ,Park SJ,Lee KH,Seok YJ

更新日期：2010-11-19 00:00:00

abstract：:The MAP kinase module (Raf/MAPKKK-MAPKK-MAPK) has been shown to be sequentially activated after mitogenic stimulation. Here we demonstrate, by site directed mutagenesis, that MAPK is able to retrophosphorylate its own activator, MAPKK, on two threonine residues Thr-292 and Thr-386 in vitro, and that these sites are al...

journal_title：FEBS letters

authors： Brunet A,Pagès G,Pouysségur J

更新日期：1994-06-13 00:00:00

abstract：:D-Aspartate increases human chorionic gonadotropin-induced testosterone production in purified rat Leydig cells. L-Aspartate, D-,L-glutamate or D-,L-asparagine could not substitute for D-aspartate and this effect was independent of glutamate receptor activation. Testosterone production was enhanced only in cells cultu...

journal_title：FEBS letters

authors： Nagata Y,Homma H,Lee JA,Imai K

更新日期：1999-02-12 00:00:00

abstract：:In cultured rat pituitary gonadotrophs, gonadotropin-releasing hormone (GnRH) induces rapid hyperpolarization of the cell membrane and causes cessation of the spontaneous electrical activity present in non-stimulated cells. This initial response to GnRH is followed by slow oscillations of membrane potential (Vm) which...

journal_title：FEBS letters

authors： Kukuljan M,Stojilković SS,Rojas E,Catt KJ

更新日期：1992-04-13 00:00:00

abstract：:The glycogen-binding (G) subunit of protein phosphatase-1G is phosphorylated stoichiometrically by glycogen synthase kinase-3 (GSK3), and with a greater catalytic efficiency than glycogen synthase, but only after prior phosphorylation by cyclic AMP-dependent protein kinase (A-kinase) at site 1. The residues phosphoryl...

journal_title：FEBS letters

authors： Dent P,Campbell DG,Hubbard MJ,Cohen P

更新日期：1989-05-08 00:00:00

abstract：:We have previously described a partial cDNA sequence encoding a RhoGAP protein, GAP25 that is homologous to the recently reported ArhGAP9 and ArhGAP12. We now describe a related new member ArhGAP15 that shares a number of domain similarities, including a pleckstrin homology (PH) domain, a RhoGAP domain and a novel mot...

journal_title：FEBS letters

authors： Seoh ML,Ng CH,Yong J,Lim L,Leung T

更新日期：2003-03-27 00:00:00

abstract：:Biotin synthase catalyses the last step of the biosynthesis of biotin in microorganisms and plants. The active protein isolated from Bacillus sphaericus and Escherichia coli contains an iron-sulphur (FeS) cluster. The native enzymes were depleted of their iron and inorganic sulphide and the resulting apoenzymes were c...

journal_title：FEBS letters

authors： Bui BT,Florentin D,Fournier F,Ploux O,Méjean A,Marquet A

更新日期：1998-11-27 00:00:00

abstract：:Glycogen phosphorylase b at concentrations close to those found in skeletal muscle interacts with sarcoplasmic reticulum membranes, but not with liposomes made of lipids extracted from these membranes, and is inhibited upon binding to the membrane. The interaction of glycogen phosphorylase with the sarcoplasmic reticu...

journal_title：FEBS letters

authors： Cuenda A,Centeno F,Gutierrez-Merino C

更新日期：1991-06-03 00:00:00

abstract：:Magainins are a family of antimicrobial peptides present in the skin extracts of Xenopus laevis. Both magainin-1 and -2 do not have any significant effect on the activity of protein kinase C (PKC). Magainin-2 was found to be readily phosphorylated by PKC to 0.5 mol 32P/mol of peptide. Neither magainin-1, which has a s...

journal_title：FEBS letters

authors： Nakabayashi H,Brown JH,Morell JL,Chen HC,Huang KP

更新日期：1990-07-02 00:00:00

abstract：:Seven peptides from subunit 9 (S9) of the human 26S proteasome were sequenced and this information was used to clone a HeLa cDNA that encodes the 46 kDa subunit. Rabbit polyclonal antisera were made against a ubiquitin fusion protein containing 12 amino acids from S9 and against a full-length S9 expressed in E. coli. ...

journal_title：FEBS letters

authors： Hoffman L,Rechsteiner M

更新日期：1997-03-10 00:00:00

abstract：:Lysosomal/phagosomal membranes of mammalian cells are coated by highly conserved glycoproteins (lamps) that are thought to protect the membranes from degradation. Interestingly, we identified an amino acid sequence in human lamp-1 characteristic of a cleavage site for the Neisseria gonorrhoeae IgA1 protease. Furthermo...

journal_title：FEBS letters

authors： Hauck CR,Meyer TF

更新日期：1997-03-17 00:00:00

abstract：:Human legumain was characterized following overexpression in a murine cell line as the C-terminal Ig-fusion protein. Upon acid treatment, the prolegumain autoproteolyzed distal to two aspartic acid residues to yield a highly active form. The ability of mature legumain to cleave after aspartic acid residues was confirm...

journal_title：FEBS letters

authors： Halfon S,Patel S,Vega F,Zurawski S,Zurawski G

更新日期：1998-10-30 00:00:00

abstract：:The antigenic cross-reactivity between purified chick, eel and mouse electrolectins (endogenous beta-D-galactoside specific lectins) have been studied using a solid phase radioimmunoassay. The immune serum raised against the eel electrolectin crossreacts both with the chick and the mouse electrolectins, while the anti...

journal_title：FEBS letters

authors： Levi G,Teichberg VI

更新日期：1982-11-01 00:00:00

abstract：:The OxyR protein is a transcriptional activator for a subset of peroxide stress-inducible genes, most of which are involved in defense systems against oxidative stress. Recently, it was demonstrated that purified OxyR has one intramolecular disulfide bond, which led to the proposal that the reversible disulfide bond f...

journal_title：FEBS letters

authors： Tao K

更新日期：1999-08-20 00:00:00

abstract：:A major house dust mite allergen Der f 1 belongs to the papain-like cysteine protease family. This study investigated whether Der f 1 can cleave the latency-associated peptide (LAP) of transforming growth factor (TGF)-beta via its proteolytic activity and activate latent TGF-beta. We found that Der f 1 can cleave LAP ...

journal_title：FEBS letters

authors： Nakamura Y,Miyata M,Shimokawa N,Ohnuma Y,Katoh R,Ogawa H,Okumura K,Nakao A

更新日期：2009-06-18 00:00:00

abstract：:Following the biophysical analysis of plant K+ channels in their natural environment, three members from the green branch of the evolutionary tree of life KAT1, AKT1, and KST1 have recently been identified on the molecular level. Among them, we focussed on the expression and characterization of the Arabidopsis thalian...

journal_title：FEBS letters

authors： Marten I,Gaymard F,Lemaillet G,Thibaud JB,Sentenac H,Hedrich R

更新日期：1996-02-19 00:00:00

abstract：:G protein-coupled receptor kinase 2 (GRK2) plays a key role in determining the rate and extent of G protein-coupled receptor (GPCR) desensitization and resensitization. Recent data indicate that GRK2 activity, subcellular distribution and expression are tightly regulated. The important physiological function of GRK2 a...

journal_title：FEBS letters

authors： Aragay AM,Ruiz-Gómez A,Penela P,Sarnago S,Elorza A,Jiménez-Sainz MC,Mayor F Jr

更新日期：1998-06-23 00:00:00

abstract：:Autophagy is a bulk degradation process conserved among eukaryotes. In macro-autophagy, autophagosomes sequester cytoplasmic components and deliver their contents to lysosomes/vacuoles. Autophagosome formation requires the conjugation of Atg8, a ubiquitin-like protein, to phosphatidylethanolamine (PE). Here we report ...

journal_title：FEBS letters

authors： Hanada T,Satomi Y,Takao T,Ohsumi Y

更新日期：2009-04-02 00:00:00

abstract：:Re-analysis of the primary structure of the ribosomal RNA N-methyltransferase that confers self-resistance on the erythromycin-producing bacterium Saccharopolyspora erythraea has confirmed the presence of a C-terminal domain containing extensive repeat sequences. Nine tandem repeats can be discerned, with a decapeptid...

journal_title：FEBS letters

authors： Dhillon N,Leadlay PF

更新日期：1990-03-26 00:00:00

abstract：:This study describes for the first time the amphiphilicity of the protein moiety of proteoglycogen. Glycogenin but not proteoglycogen associates to phospholipid vesicles and forms by itself stable Gibbs and Langmuir monolayers at the air-buffer interface. The adsorption free energy (-6.7 kcal/mol) and the glycogenin c...

journal_title：FEBS letters

authors： Carrizo ME,Miozzo MC,Maggio B,Curtino JA

更新日期：2001-12-07 00:00:00

abstract：:The activity of the Ca-pumping ATPase of cardiac sarcoplasmic reticulum (SR) is controlled by the phosphorylation of the intrinsic regulatory protein phospholamban (PLB), which affects both the apparent Km(Ca) and the Vmax of the transport process. We have investigated the correlation between phosphorylation of PLB an...

journal_title：FEBS letters

authors： Chiesi M,Schwaller R

更新日期：1989-02-13 00:00:00

abstract：:A series of 9-beta-D-xylofuranosyladenine (xyloA or xyloadenosine) substituted analogs of 2-5A core trimer and tetramer were examined for their ability to be degraded by the 2',5'-phosphodiesterase activity of cytoplasmic extracts of mouse L cells. Two distinct groups of xyloA-substituted analogs could be readily disc...

journal_title：FEBS letters

authors： Torrence PF,Alster D,Huss S,Gosselin G,Imbach JL

更新日期：1987-02-23 00:00:00

abstract：:A novel member of the human UDP-N-acetyl-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase gene family, designated GalNAc-T7, was cloned and expressed. GalNAc-T7 exhibited different properties compared to other characterized members of this gene family, in showing apparent exclusive specificity for partial...

journal_title：FEBS letters

authors： Bennett EP,Hassan H,Hollingsworth MA,Clausen H

更新日期：1999-10-29 00:00:00

abstract：:We have recently developed a method to produce native human proinsulin using a bacterial expression system. A proinsulin fusion protein was recovered from inclusion bodies and cleaved using cyanogen bromide. The released proinsulin polypeptide was S-sulfonated and purified by anion exchange chromatography. Following r...

journal_title：FEBS letters

authors： Cowley DJ,Mackin RB

更新日期：1997-02-03 00:00:00

abstract：:A bovine anterior pituitary polypeptide that stimulates the replication of rat and human adipocyte precursors has been purified. Its Mr is 44 000-53 000 and its isoelectric point is 9.8-10.3. While pituitary basic fibroblast growth factor is equally mitogenic on adipocyte precursors and skin fibroblasts, the polypepti...

journal_title：FEBS letters

authors： Lau DC,Roncari DA,Yip DK,Kindler S,Nilsen SG

更新日期：1983-03-21 00:00:00

abstract：:Many biochemical reactions are confined to interfaces, such as membranes or cell walls. Despite their importance, no canonical rate laws describing the kinetics of surface-active enzymes exist. Combining the approach chosen by Michaelis and Menten 100 years ago with concepts from surface chemical physics, we here pres...

journal_title：FEBS letters

authors： Kartal O,Ebenhöh O

更新日期：2013-09-02 00:00:00

abstract：:DNA complementary to the rat hypothalamic mRNA coding for the corticotropin-releasing factor precursor (prepro-CRF) has been cloned by screening a cDNA library with a human genomic DNA probe. Nucleotide sequence analysis of the cloned cDNA has revealed that rat prepro-CRF consists of 187 amino acid residues including ...

journal_title：FEBS letters

authors： Jingami H,Mizuno N,Takahashi H,Shibahara S,Furutani Y,Imura H,Numa S

更新日期：1985-10-21 00:00:00

abstract：:Fructose-1,6-bisphosphatase, a key enzyme in gluconeogenesis, undergoes catabolite inactivation when glucose is added to gluconeogenetically active cells of the yeast Saccharomyces cerevisiae. Phosphorylation of the enzyme is followed by rapid degradation. To elucidate the cellular proteolytic system involved in catab...

journal_title：FEBS letters

authors： Schork SM,Bee G,Thumm M,Wolf DH

更新日期：1994-08-01 00:00:00

abstract：:The activation of peroxisome proliferator-activated receptor gamma (PPARgamma) has been shown to inhibit the production and the effects of proinflammatory cytokines. Since interleukin-1beta (IL-1beta) directly mediates cartilage degradation in osteoarthritis, we investigated the capability of PPARgamma ligands to modu...

journal_title：FEBS letters

authors： Boyault S,Simonin MA,Bianchi A,Compe E,Liagre B,Mainard D,Bécuwe P,Dauça M,Netter P,Terlain B,Bordji K

更新日期：2001-07-13 00:00:00