Functional expression of the plant K+ channel KAT1 in insect cells.


:Following the biophysical analysis of plant K+ channels in their natural environment, three members from the green branch of the evolutionary tree of life KAT1, AKT1, and KST1 have recently been identified on the molecular level. Among them, we focussed on the expression and characterization of the Arabidopsis thaliana K+ channel KAT1 in the insect cell line Sf9. The infection of Sf9 cells with KAT1-recombinant baculovirus resulted in functional expression of KAT1 channels, which was monitored by inward-rectifying, K+-selective (impermeable to Na+ and even NH4+) ionic conductance in whole-cell patch-clamp recordings. A voltage threshold as low as -60 to -80mV for voltage activation compared to other plant inward rectifiers in vivo, and to in vitro expression of KAT1 in Xenopus oocytes or yeast, may be indicative for channel modulation by the expression system. A rise in cytoplasmic Ca2+ concentration (up to 1 mM), a regulator of the inward rectifier in Vicia faba guard cells, did not modify the voltage dependence of KAT1 in Sf9 cells. The access to channel function on one side and channel protein on the other make Sf9 cells a suitable heterologous system for studies on the biophysical properties, post-traditional modification and assembly of a green inward rectifier.




FEBS letters


Marten I,Gaymard F,Lemaillet G,Thibaud JB,Sentenac H,Hedrich R




Has Abstract


1996-02-19 00:00:00














  • Structural characteristics and refolding of in vivo aggregated hyperthermophilic archaeon proteins.

    abstract::Several recombinant proteins in inclusion bodies expressed in Escherichia coli have been measured by Fourier transform infrared and solid-state nuclear magnetic resonance spectra to provide the secondary structural characteristics of the proteins from hyperthermophilic archaeon Pyrococcus horikoshii OT3 (hyperthermoph...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Umetsu M,Tsumoto K,Ashish K,Nitta S,Tanaka Y,Adschiri T,Kumagai I

    更新日期:2004-01-16 00:00:00

  • Ser727-dependent transcriptional activation by association of p300 with STAT3 upon IL-6 stimulation.

    abstract::Activation of the signal transducer and activator of transcription 3 (STAT3) in response to interleukin-6 (IL-6) type cytokines involves both phosphorylation of Tyr705, which enables dimerization, nuclear translocation and DNA binding, as well as ser727 phosphorylation. Here, we describe that the 65 C-terminal amino a...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Schuringa JJ,Schepers H,Vellenga E,Kruijer W

    更新日期:2001-04-20 00:00:00

  • RORalpha1 and RORalpha4 suppress TNF-alpha-induced VCAM-1 and ICAM-1 expression in human endothelial cells.

    abstract::Retinoic acid receptor-related orphan receptor-alpha (RORalpha) is a nuclear orphan receptor. Adenovirus-mediated overexpression of RORalpha1 and RORalpha4 suppressed tumor necrosis factor-alpha (TNF-alpha)-induced expression of vascular cell adhesion molecule-1 (VCAM-1) and intracellular adhesion molecule-1 (ICAM-1) ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Migita H,Satozawa N,Lin JH,Morser J,Kawai K

    更新日期:2004-01-16 00:00:00

  • Structural modelling of the complex of leucyl-tRNA synthetase and mis-aminoacylated tRNALeu.

    abstract::To assure fidelity of translation, class Ia aminoacyl-tRNA synthetases (aaRSs) edit mis-aminoacylated tRNAs. Mis-attached amino acids and structural water molecules are not included simultaneously in the current crystal structures of the aaRSctRNA complexes, where the 3'-ends (adenine 76; A76) are bound to the editing...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Hagiwara Y,Nureki O,Tateno M

    更新日期:2009-02-18 00:00:00

  • A role for the octameric ring protein, Translin, in mitotic cell division.

    abstract::The octameric ring protein, Translin, demonstrates marked similarities to the family of helicase enzymes regarding its quaternary organization and dimerization of subunits. Here we show that the level of Translin closely parallels the proliferative state in various cell types. Expression is periodic during the cell cy...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Ishida R,Okado H,Sato H,Shionoiri C,Aoki K,Kasai M

    更新日期:2002-08-14 00:00:00

  • Application of modular kinetic analysis to mitochondrial oxidative phosphorylation in skeletal muscle of birds exposed to acute heat stress.

    abstract::We previously showed that heat stress stimulates reactive oxygen species (ROS) production in skeletal muscle mitochondria of birds, probably via an elevation in mitochondrial membrane potential (DeltaPsi). To clarify the mechanism underlying the elevation of DeltaPsi, modular kinetic analysis was applied to oxidative ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Kikusato M,Ramsey JJ,Amo T,Toyomizu M

    更新日期:2010-07-16 00:00:00

  • Human manganese superoxide dismutase suppresses HER2/neu-mediated breast cancer malignancy.

    abstract::The up-regulation of HER2/neu is associated with human malignancies and is a useful target for developing anticancer drugs. Overexpression of human manganese superoxide dismutase (MnSOD) has been demonstrated to effectively suppress various carcinoma cells, including breast carcinomas, in vitro and in vivo. This study...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Chuang TC,Liu JY,Lin CT,Tang YT,Yeh MH,Chang SC,Li JW,Kao MC

    更新日期:2007-09-18 00:00:00

  • Stimulation of nuclear sphingosine kinase activity by platelet-derived growth factor.

    abstract::Subcellular fractionation revealed that a significant fraction of total sphingosine kinase, the enzyme that phosphorylates sphingosine to form the bioactive lipid metabolite sphingosine-1-phosphate, resides in the nuclei of Swiss 3T3 cells, localized to both the nuclear envelope and the nucleoplasm. Platelet-derived g...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Kleuser B,Maceyka M,Milstien S,Spiegel S

    更新日期:2001-08-10 00:00:00

  • Expression of CYP2S1 in human hepatic stellate cells.

    abstract::Activated stellate cells are myofibroblast-like cells associated with the generation of fibrotic scaring in chronically damaged liver. Gene chip analysis was performed on cultured fibrotic stellate cells. Of the 51 human CYP genes known, 13 CYP and 5 CYP reduction-related genes were detected with 4 CYPs (CYP1A1, CYP2E...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Marek CJ,Tucker SJ,Koruth M,Wallace K,Wright MC

    更新日期:2007-02-20 00:00:00

  • MIG-13 controls anteroposterior cell migration by interacting with UNC-71/ADM-1 and SRC-1 in Caenorhabditis elegans.

    abstract::The transmembrane protein MIG-13 is a key regulator required for anterior migration of neural cells in Caenorhabditis elegans, but the signaling mechanisms involved remain unknown. Here, we isolated a suppressor mutation in the unc-71/adm-1 gene, which rescued the AVM neuron migration defect in mig-13 mutants. Genetic...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Masuda H,Nakamura K,Takata N,Itoh B,Hirose T,Moribe H,Mekada E,Okada M

    更新日期:2012-03-23 00:00:00

  • Transient suppression of PPARgamma directed ES cells into an osteoblastic lineage.

    abstract::Osteoblasts and adipocytes are believed to share a common progenitor. Peroxisome proliferator-activated receptor gamma (PPARgamma) plays a key role in the switching of these two cell lineages. Here, we demonstrated the differentiation of ES cells into an osteoblastic lineage using siRNA against PPARgamma without the a...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Yamashita A,Takada T,Nemoto K,Yamamoto G,Torii R

    更新日期:2006-07-24 00:00:00

  • Cyclosporin A inhibits H2O2-induced apoptosis of human fibroblasts.

    abstract::Several clinical studies have shown that cyclosporin A (CsA) is effective for treating a variety of chronic inflammatory and autoimmune diseases. Because reactive oxygen species are believed to play a key role in the development of these diseases, causing cell apoptosis, we investigated whether CsA inhibits H2O2-induc...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Sugano N,Ito K,Murai S

    更新日期:1999-03-26 00:00:00

  • Replication fork pausing at protein barriers on chromosomes.

    abstract::When a cell divides prior to completion of DNA replication, serious DNA damage may occur. Thus, in addition to accuracy, the processivity of the replication forks is important. DNA synthesis at replication forks should be completed in time, and forks overcome aberrant structures on the template DNA, including damaged ...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审


    authors: Hizume K,Araki H

    更新日期:2019-07-01 00:00:00

  • Cellular RNA is not degraded in interferon-treated HeLa cells after poliovirus infection.

    abstract::A drastic inhibition of protein synthesis occurs in HeLa cells treated with human lymphoblastoid interferon and infected with poliovirus. At the time when this inhibition has been established no degradation of 32P-labelled ribosomal RNA can be detected. Isolation of the mRNAs from poliovirus-infected cells plus or min...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Muñoz A,Harvey R,Carrasco L

    更新日期:1983-08-22 00:00:00

  • Addition of a methyl group changes both the catalytic velocity and thermostability of the neutral protease from Bacillus stearothermophilus.

    abstract::Specific activity was compared between wild-type (WT) neutral protease from Bacillus stearothermophilus and mutant protease (M1; Gly144 replaced by Ala144) with enhanced thermostability. When casein was used as a substrate, M1 showed 1.5-times higher specific activity than that of WT. In contrast, the specific activit...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Takagi M,Imanaka T

    更新日期:1989-08-28 00:00:00

  • Ceramide generated by acidic sphingomyelinase contributes to tumor necrosis factor-alpha-mediated apoptosis in human colon HT-29 cells through glycosphingolipids formation. Possible role of ganglioside GD3.

    abstract::In the present study we assessed the contribution of acidic sphingomyelinase (ASMase), a ceramide generating enzyme, in tumor necrosis factor (TNF)-mediated apoptosis in human colon HT-29 cells. TNF induced apoptosis in HT-29 cells in a time- and dose-dependent fashion. Downregulation of the active endogenous ASMase f...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Colell A,Morales A,Fernández-Checa JC,García-Ruiz C

    更新日期:2002-08-28 00:00:00

  • Ubiquitin gene expression is increased in skeletal muscle of tumour-bearing rats.

    abstract::Rats bearing the fast-growing AH-130 Yoshida ascites hepatoma showed a marked cachectic response which has been previously reported [Tessitore et al. (1987) Biochem. J. 241, 153-159]. Thus tumour-bearing animals showed significant decreases in body and muscle weight (soleus and gastrocnemius) as compared to both pair-...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Llovera M,García-Martínez C,Agell N,Marzábal M,López-Soriano FJ,Argilés JM

    更新日期:1994-02-07 00:00:00

  • A novel coumarin-labelled peptide for sensitive continuous assays of the matrix metalloproteinases.

    abstract::(7-methoxycoumarin-4-yl)Acetyl-Pro-Leu-Gly-Leu-(3-[2,4-dinitrophenyl]-L- 2,3-diaminopropionyl)-Ala-Arg-NH2 (Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2) has been synthesised as a fluorogenic substrate for the matrix metalloproteinases. The highly fluorescent 7-methoxycoumarin group is efficiently quenched by energy transfer t...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Knight CG,Willenbrock F,Murphy G

    更新日期:1992-01-27 00:00:00

  • Expression and characterization of VIP and two VIP mutants in NIH 3T3 cells.

    abstract::Prepro-vasoactive intestinal peptide (prepro VIP) was expressed in NIH 3T3 cells, and the prepro VIP-derived peptides produced by the cells were analyzed by chromatography combined with sequence-specific radio-immunoanalysis. In accordance with what has previously been reported on processing in non-endocrine cell line...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Wulff BS,Georg B,Fahrenkrug J

    更新日期:1994-03-14 00:00:00

  • GSK3 alpha and GSK3 beta are necessary for axon formation.

    abstract::The mechanisms that underlie axon formation are still poorly understood. GSK3 has been recently implicated in establishing the axon and in its elongation. We have used four different GSK3 inhibitors to determine the role of GSK3 activity in hippocampal neurons at different periods of time. Inhibition of GSK3 activity ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Garrido JJ,Simón D,Varea O,Wandosell F

    更新日期:2007-04-17 00:00:00

  • A mutation affecting carbon catabolite repression suppresses growth defects in pyruvate carboxylase mutants from Saccharomyces cerevisiae.

    abstract::Yeasts with disruptions in the genes PYC1 and PYC2 encoding the isoenzymes of pyruvate carboxylase cannot grow in a glucose-ammonium medium (Stucka et al. (1991) Mol. Gen. Genet. 229, 307-315). We have isolated a dominant mutation, BPC1-1, that allows growth in this medium of yeasts with interrupted PYC1 and PYC2 gene...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Blázquez MA,Gamo FJ,Gancedo C

    更新日期:1995-12-18 00:00:00

  • Comparing the temperature dependence of FMN to heme electron transfer in full length and truncated inducible nitric oxide synthase proteins.

    abstract::The FMN-heme interdomain (intraprotein) electron transfer (IET) kinetics in full length and oxygenase/FMN (oxyFMN) construct of human iNOS were determined by laser flash photolysis over the temperature range from 283 to 304K. An appreciable increase in the rate constant value was observed with an increase in the tempe...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Li W,Chen L,Fan W,Feng C

    更新日期:2012-01-20 00:00:00

  • Volume-regulatory taurine release from a human lung cancer cell line. Evidence for amino acid transport via a volume-activated chloride channel.

    abstract::Exposure of a human lung epithelial cancer cell-line to hypo-osmotic media led to a marked increase in the rate of efflux from the cells of taurine, a non-essential sulfonic amino acid. The osmotically-activated taurine efflux was inhibited by a range of known Cl- channel blockers, the most potent of which were NPPB a...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Kirk K,Kirk J

    更新日期:1993-12-20 00:00:00

  • Deletion of highly conserved C-terminal sequences in the Kv1 K+ channel sub-family does not prevent expression of currents with wild-type characteristics.

    abstract::The C-terminal regions of Kv1 K+ channels show little conservation between isoforms except for the last four C-terminal residues, (E/L)TDV, which are well conserved from Drosophila to man. Deletions of the 4, 16, and 57 C-terminal residues of the human Kv1.5 channel did not affect whole cell current amplitude, midpoin...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Uebele VN,Yeola SW,Snyders DJ,Tamkun MM

    更新日期:1994-02-28 00:00:00

  • Use of monoclonal antibodies to analyse the expression of a multi-tubulin family.

    abstract::We have used a panel of monoclonal antibodies in a study of the expression of multiple tubulins in Physarum polycephalum. Three anti-beta-tubulin monoclonal antibodies, DM1B, DM3B3 and KMX-1 all reacted with the beta 1-tubulin isotypes expressed in both myxamoebae and plasmodia. However, these antibodies showed a spec...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Birkett CR,Foster KE,Johnson L,Gull K

    更新日期:1985-08-05 00:00:00

  • Jacalin interacts with Asn-linked glycopeptides containing multi-antennary oligosaccharide structure with terminal alpha-linked galactose.

    abstract::The carbohydrate binding properties of jacalin lectin were examined using RAF9 cell-derived D-[6-3H]glucosamine-radiolabeled total glycopeptides containing N-linked and O-linked oligosaccharides. The binding of N-linked glycopeptides to jacalin was abolished by treatment of alpha-galactosidase whereas O-linked glycope...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Do SI,Lee KY

    更新日期:1998-01-09 00:00:00

  • The functional role of the beta-subunit in the maturation and intracellular transport of Na,K-ATPase.

    abstract::The minimal functional enzyme unit of Na,K-ATPase consists of an alpha-beta complex. The alpha-subunit bears all functional domains of the enzyme and so far a regulatory role for the beta-subunit in the catalytic cycle has not been established. On the other hand, increasing experimental evidence suggests that the beta...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审


    authors: Geering K

    更新日期:1991-07-22 00:00:00

  • Interaction of smooth muscle calponin with phospholipids.

    abstract::Analyzing the primary structure we predicted that calponin may interact with phospholipids. In order to check this suggestion we investigated the interaction of calponin with phospholipids by ultracentrifugation, light scattering, vesicles leakage and differential scanning calorimetry. In agreement with our prediction...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Bogatcheva NV,Gusev NB

    更新日期:1995-09-04 00:00:00

  • alphaIIb beta 3-integrin mediated adhesion of human platelets to a fibrinogen matrix triggers phospholipase C activation and phosphatidylinositol 3',4'-biphosphate accumulation.

    abstract::This study focused on the variations in phosphoinositide metabolism depending upon alphaIIbbeta3-integrin/fibrinogen interaction without previous activation of platelet agonist receptors. We found that adhesion of resting human platelets to immobilized fibrinogen stimulates phosphatidic acid production and a concomita...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Gironcel D,Racaud-Sultan C,Payrastre B,Haricot M,Borchert G,Kieffer N,Breton M,Chap H

    更新日期:1996-07-08 00:00:00

  • NMR determination of the secondary structure and the three-dimensional polypeptide backbone fold of the human sterol carrier protein 2.

    abstract::Nuclear magnetic resonance (NMR) spectroscopy was used to determine the secondary structure and the three-dimensional polypeptide backbone fold of the human sterol carrier protein 2 (hSCP2), which is a basic protein with 123 residues believed to participate in the intracellular transport of cholesterol and various oth...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Szyperski T,Scheek S,Johansson J,Assmann G,Seedorf U,Wüthrich K

    更新日期:1993-11-29 00:00:00