Induction of caspase-11 by inflammatory stimuli in rat astrocytes: lipopolysaccharide induction through p38 mitogen-activated protein kinase pathway.

abstract：:The predominance of oxidative stress in many tumour cell environments provides a means to selectively target these cells via protein oxidation. The zinc fingers of transcription factors utilise cysteine thiols for structural zinc coordination. Redox control of DNA binding regulates transcription and therefore the over...

journal_title：FEBS letters

authors： Giles NM,Gutowski NJ,Giles GI,Jacob C

更新日期：2003-01-30 00:00:00

abstract：:Recombinant human interleukin-5 (hIL-5) has been expressed at high levels and produced in large quantities in baculovirus infected Sf9 insect cells. The glycosylated protein was purified using immuno-affinity chromatography and gel filtration. Purified hIL-5 has been crystallized using standard vapour diffusion techni...

journal_title：FEBS letters

authors： Guisez Y,Oefner C,Winkler FK,Schlaeger EJ,Zulauf M,Van der Heyden J,Plaetinck G,Cornelis S,Tavernier J,Fiers W

更新日期：1993-09-27 00:00:00

abstract：:Ovine trophoblastic protein B (oTPB), an embryonic protein, is a 20 kDa secretory protein which is synthesized by the ovine conceptus from days 12 to 22 of pregnancy. oTPB was purified by HPLC using ion-exchange chromatography on a DEAE column and was subsequently chromatographed on a reversed-phase column. Automated ...

journal_title：FEBS letters

authors： Charpigny G,Reinaud P,Huet JC,Guillomot M,Charlier M,Pernollet JC,Martal J

更新日期：1988-02-08 00:00:00

abstract：:The effect of all possible codons corresponding to the second and third amino acid (isoleucine and threonine) on the expression level of hirudin in E. coli has been analysed. These levels could not be correlated with changes in primary and secondary mRNA structure. A decrease in the rate of synthesis and of product ac...

journal_title：FEBS letters

authors： Degryse E

更新日期：1990-08-20 00:00:00

abstract：:Respiratory complexes in both prokaryotes and eukaryotes contain multiple co-factors, which are coordinated in defined positions so that they can function as electron wires. Intriguingly, co-factors are usually buried deep within hetero-oligomeric protein complexes and it is not clear when or how they are incorporated...

journal_title：FEBS letters

authors： Palombo I,Daley DO

更新日期：2012-11-30 00:00:00

abstract：:In HCT116 colorectal cancer cells, HeLa cervical cancer cells and HuH-7 hepatoma cells, miR-223 is expressed at a low level. Through infection with lentivirus containing miR-223 precursor, miR-223 [corrected] was overexpressed in all these cells. Interestingly, the expression levels of FOXO1 mRNA and protein, and phos...

journal_title：FEBS letters

authors： Wu L,Li H,Jia CY,Cheng W,Yu M,Peng M,Zhu Y,Zhao Q,Dong YW,Shao K,Wu A,Wu XZ

更新日期：2012-04-05 00:00:00

abstract：:Recently, we have reported the presence of kinetin (N6-furfuryladenine) in commercially available DNA, in freshly extracted cellular DNA and in plant cell extracts. We have also found that kinetin has electrochemical properties which can be used for monitoring the level of this modified base in DNA. Here, for the firs...

journal_title：FEBS letters

authors： Barciszewski J,Siboska GE,Pedersen BO,Clark BF,Rattan SI

更新日期：1997-09-08 00:00:00

abstract：:DNA complementary to chicken ultimobranchial gland mRNA was cloned into the Pst I site of plasmid vector pBR322. A plasmid was selected by DNA-mRNA hybridization. We report here the partial nucleotide sequence of chicken calcitonin mRNA and the deduced complete amino acid sequence of chicken calcitonin. ...

journal_title：FEBS letters

authors： Lasmoles F,Jullienne A,Desplan C,Milhaud G,Moukhtar MS

更新日期：1985-01-21 00:00:00

abstract：:Three site-directed mutants of human epidermal growth factor, Leu-26----Gly, Leu-47----Ala, and Ile-23----Thr, were examined for their ability to stimulate the protein-tyrosine kinase activity of the epidermal growth factor receptor. The receptor binding affinities of the mutant growth factors were 20- to 50-fold lowe...

journal_title：FEBS letters

authors： Matsunami RK,Campion SR,Niyogi SK,Stevens A

更新日期：1990-05-07 00:00:00

abstract：:The labile iron pool of cells (LIP) constitutes the primary source of metabolic and catalytically reactive iron in the cytosol. We studied LIP homeostasis in K562 cells using the fluorescent metal-sensitive probe calcein. Following brief exposure to iron(II) salts or to oxidative or reductive stress, LIP rose by up to...

journal_title：FEBS letters

authors： Breuer W,Epsztejn S,Cabantchik ZI

更新日期：1996-03-18 00:00:00

abstract：:We present here a combined GC-MS and LC-MS metabolic profiling approach to unraveling the pathological outcomes of aristolochic acid (AA)-induced nephrotoxicity. Urine samples were analyzed by GC-MS and LC-MS in combination with pattern recognition techniques, e.g. principal component analysis (PCA), orthogonal projec...

journal_title：FEBS letters

authors： Ni Y,Su M,Qiu Y,Chen M,Liu Y,Zhao A,Jia W

更新日期：2007-02-20 00:00:00

abstract：:omega-Conotoxin MVIIC binds to P/Q-type calcium channels with high affinity and N-type channels with low affinity. To reveal the residues essential for subtype selectivity, we synthesized Ala-scanning analogs of MVIIC. Binding assays using rat cerebellar P(2) membranes suggested that Thr(11), Tyr(13) and Lys(2) are es...

journal_title：FEBS letters

authors： Sato K,Raymond C,Martin-Moutot N,Sasaki T,Ohtake A,Minami K,Van Renterghem C,Kim JI,Takahashi M,Seagar MJ

更新日期：2000-03-10 00:00:00

abstract：:Long-chain fatty acid amides are cell-signaling lipids identified in mammals and, recently, in invertebrates, as well. Many details regarding fatty acid amide metabolism remain unclear. Herein, we demonstrate that Drosophila melanogaster is an excellent model system for the study long-chain fatty acid amide metabolism...

journal_title：FEBS letters

authors： Jeffries KA,Dempsey DR,Behari AL,Anderson RL,Merkler DJ

更新日期：2014-05-02 00:00:00

abstract：:A complete cDNA clone encoding human cholesterol 7 alpha-hydroxylase has been isolated using a rat P-450ch7 alpha cDNA insert [(1989) FEBS Lett. 257, 97-100] as a probe and totally sequenced. The cDNA contained 1512-base pair open reading frame encoding 504 amino acid residues (Mr 57,630), 39-base pair 5'-untranslated...

journal_title：FEBS letters

authors： Noshiro M,Okuda K

更新日期：1990-07-30 00:00:00

abstract：:Several recombinant proteins in inclusion bodies expressed in Escherichia coli have been measured by Fourier transform infrared and solid-state nuclear magnetic resonance spectra to provide the secondary structural characteristics of the proteins from hyperthermophilic archaeon Pyrococcus horikoshii OT3 (hyperthermoph...

journal_title：FEBS letters

authors： Umetsu M,Tsumoto K,Ashish K,Nitta S,Tanaka Y,Adschiri T,Kumagai I

更新日期：2004-01-16 00:00:00

abstract：:The actin-depolymerising factor (ADF)/cofilin group of proteins are stimulus-responsive actin-severing proteins, members of which are regulated by reversible phosphorylation. The phosphorylation site on the maize ADF, ZmADF3, is Ser-6 but the kinase responsible is unknown [Smertenko et al., Plant J. 14 (1998) 187-193]...

journal_title：FEBS letters

authors： Allwood EG,Smertenko AP,Hussey PJ

更新日期：2001-06-15 00:00:00

abstract：:Polyamine depletion, obtained in FAO cells with specific inhibitors of biosynthetic enzymes, prevents or decreases the accumulation of hsp 70 mRNA following heat shock [Desiderio et al., Hepatology 24 (1996) 150-156]. The present study shows that under conditions of spermidine depletion caused by alpha-difluoromethylo...

journal_title：FEBS letters

authors： Desiderio MA,Dansi P,Tacchini L,Bernelli-Zazzera A

更新日期：1999-07-16 00:00:00

abstract：:Neprilysin-2 (NEP2) is a novel metallopeptidase homologous to neprilysin (NEP), an enzyme involved in regulation of neuropeptide signalling. NEP2 exists as two alternatively spliced isoforms, NEP2 and NEP2(Delta). In this study, we cloned and expressed both human isoforms. Human NEP2 exists as a membrane-bound and sol...

journal_title：FEBS letters

authors： Whyteside AR,Turner AJ

更新日期：2008-07-09 00:00:00

abstract：:A point mutation (Ala-589 to Thr) in the transmembrane protein of the human immunodeficiency virus type 1 (HIV-1) has been shown to decrease the sensitivity of the virus to the neutralizing effect of human HIV-1 specific antibodies [(1990) J. Virol. 64, 3240-3248]. Here 17-residue peptides with the parental and mutant...

journal_title：FEBS letters

authors： Klasse J,Pipkorn R,Blomberg J,Han KH,Hilton B,Ferretti JA

更新日期：1993-05-24 00:00:00

abstract：:DNA enzymes are RNA-cleaving single-stranded DNA molecules. We designed DNA enzymes targeting the PB2 mRNA translation initiation (AUG) region of the influenza A virus (A/PR/8/34). The modified DNA enzymes have one or two N3'-P5' phosphoramidate bonds at both the 3'- and 5'-termini of the oligonucleotides, which signi...

journal_title：FEBS letters

authors： Takahashi H,Hamazaki H,Habu Y,Hayashi M,Abe T,Miyano-Kurosaki N,Takaku H

更新日期：2004-02-27 00:00:00

abstract：:The SEC10 gene product is a member of the Exocyst complex essential for exocytosis in the budding yeast Saccharomyces cerevisiae. We report here the cloning and characterization of human Sec10p (hSec10p; GenBank accession number U85946). hSec10p is a 77-kDa protein with 23% amino acid identity to yeast Sec10p and 37% ...

journal_title：FEBS letters

authors： Guo W,Roth D,Gatti E,De Camilli P,Novick P

更新日期：1997-03-10 00:00:00

abstract：:We have used a panel of monoclonal antibodies in a study of the expression of multiple tubulins in Physarum polycephalum. Three anti-beta-tubulin monoclonal antibodies, DM1B, DM3B3 and KMX-1 all reacted with the beta 1-tubulin isotypes expressed in both myxamoebae and plasmodia. However, these antibodies showed a spec...

journal_title：FEBS letters

authors： Birkett CR,Foster KE,Johnson L,Gull K

更新日期：1985-08-05 00:00:00

abstract：:Transections of the entorhinal afferent fibers to hippocampus, perforant path (PP), result in the denervation in specific hippocampal subregions, which is followed by a series of plastic events including axon sprouting and reactive synaptogenesis. Many growth-associated molecules are thought to participate in these ev...

journal_title：FEBS letters

authors： Wang Y,Ni ZM,Zhou CF

更新日期：2005-02-14 00:00:00

abstract：:Acetylcholinesterase (AChE) is likely to have roles other than the hydrolysis of acetylcholine, e.g., related to developmental processes like neurite outgrowth, differentiation and adhesion. Here, we investigated whether AChE can function as a heterophilic cell adhesion molecule and searched for proteins interacting w...

journal_title：FEBS letters

authors： Paraoanu LE,Layer PG

更新日期：2004-10-08 00:00:00

abstract：:In plants exposed to high irradiances of visible light, the D1 protein in the reaction center of photosystem II is oxidatively damaged and rapidly degraded. Earlier work in our laboratory showed that the serine protease Deg2 performs the primary cleavage of photodamaged D1 protein in vitro. Here, we demonstrate that t...

journal_title：FEBS letters

authors： Huesgen PF,Schuhmann H,Adamska I

更新日期：2006-12-22 00:00:00

abstract：:The presence of additives during X-irradiation of malate synthase led to radioprotective effects against primary and post-irradiation inactivation. Pronounced effects were provided by typical scavengers, sulfhydryl reagents and specific ligands (substrates, products, analogues). The results show that scavenging and sp...

journal_title：FEBS letters

authors： Durchschlag H,Zipper P

更新日期：1988-09-12 00:00:00

abstract：:A characteristic of the antioxidant, probucol, is its inability to inhibit apolipoprotein B fragmentation in low density lipoprotein (LDL), despite a pronounced ability to inhibit lipid oxidation on relatively lengthy exposure to Cu(II). Here we show that a short exposure of LDL to hydrogen peroxide and Cu(II) leads t...

journal_title：FEBS letters

authors： Hunt JV,Bailey JR,Schultz DL,McKay AG,Mitchinson MJ

更新日期：1994-08-08 00:00:00

abstract：:Proteasomes were purified from human hepatoma tissues, and their sensitivities to Na+ and K+ were examined. At concentrations of 10 mM or more, these cations were found to inhibit completely polylysine-activated casein degradation by the purified proteasomes. They also strongly inhibited the hydrolyses of peptides, al...

journal_title：FEBS letters

authors： Seol JH,Park SC,Ha DB,Chung CH,Tanaka K,Ichihara A

更新日期：1989-04-24 00:00:00

abstract：:Inhibition of mammalian DNA topoisomerase I by phospholipids was investigated using purified enzyme. Acidic phospholipids inhibited the DNA relaxation activity of topoisomerase I whereas neutral phospholipid, phosphatidylethanolamine, did not. Accumulation of a protein-DNA cleavable complex, an intermediate which is k...

journal_title：FEBS letters

authors： Tamura H,Ikegami Y,Ono K,Sekimizu K,Andoh T

更新日期：1990-02-12 00:00:00

abstract：:The structure of the D158E mutant of caricain (previously known as papaya protease omega) in complex with E-64 has been determined at 2.0 A resolution (overall R factor 19.3%). The structure reveals that the substituted glutamate makes the same pattern of hydrogen bonds as the aspartate in native caricain. This was no...

journal_title：FEBS letters

authors： Katerelos NA,Taylor MA,Scott M,Goodenough PW,Pickersgill RW

更新日期：1996-08-19 00:00:00