Na+, K+-specific inhibition of protein and peptide hydrolyses by proteasomes from human hepatoma tissues.


:Proteasomes were purified from human hepatoma tissues, and their sensitivities to Na+ and K+ were examined. At concentrations of 10 mM or more, these cations were found to inhibit completely polylysine-activated casein degradation by the purified proteasomes. They also strongly inhibited the hydrolyses of peptides, although to a lesser extent. On the other hand, they reversed the inhibitory and stimulatory effects of polylysine on the hydrolyses of Suc-Leu-Tyr-AMC and Cbz-Ala-Arg-Arg-MNA, respectively. These results suggest that Na+ and/or K+ may be involved in the regulation of intracellular protein breakdown by controlling the multicatalytic activity of proteasomes.




FEBS letters


Seol JH,Park SC,Ha DB,Chung CH,Tanaka K,Ichihara A




Has Abstract


1989-04-24 00:00:00














  • Studies on the proteins involved in the interaction of high-density lipoprotein with isolated human small intestine epithelial cells.

    abstract::Treatment of 125I-labelled high-density lipoprotein ([125I]HDL3) with monospecific polyclonal antibodies against apolipoproteins A-I and A-II resulted in a dose-dependent inhibition of the [125I]HDL3 binding to isolated human small intestine epithelial cells by 25% and 50%, respectively. Both antibodies also inhibited...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Sviridov DD,Ehnholm C,Tenkanen H,Pavlov MYu,Safonova IG,Repin VS

    更新日期:1992-06-01 00:00:00

  • Adiponectin deficiency suppresses ABCA1 expression and ApoA-I synthesis in the liver.

    abstract::Plasma high density lipoprotein (HDL)-cholesterol levels are inversely correlated with the incidence of cardiovascular diseases. HDL is mainly assembled in the liver through the ATP-binding cassette transporter (ABCA1) pathway. In humans, plasma HDL-cholesterol levels are positively correlated with plasma adiponectin ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Oku H,Matsuura F,Koseki M,Sandoval JC,Yuasa-Kawase M,Tsubakio-Yamamoto K,Masuda D,Maeda N,Ohama T,Ishigami M,Nishida M,Hirano K,Kihara S,Hori M,Shimomura I,Yamashita S

    更新日期:2007-10-30 00:00:00

  • Demonstration and partial purification of lactoperoxidase from human colostrum.

    abstract::A peroxidase with stability, chromatographic and immunoreactive properties similar to that of bovine lactoperoxidase has been partly purified from human colostrum. Hydrophobic interaction chromatography on Phenyl-Sepharose C1-4B gave a 10-fold purification with an apparent recovery of about 45%. The enzyme was quantit...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Langbakk B,Flatmark T

    更新日期:1984-09-03 00:00:00

  • Stable RNA interference of host thrombospondin-1 blocks Trypanosoma cruzi infection.

    abstract::Interactions between Trypanosoma cruzi and the extracellular matrix play an important role in cellular invasion. Here we show that T. cruzi increases the levels of thrombospondin-1 (TSP-1) expression in host cells during early infection. Stable RNA interference of host cell TSP-1 knocks down the levels of TSP-1 transc...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Simmons KJ,Nde PN,Kleshchenko YY,Lima MF,Villalta F

    更新日期:2006-04-17 00:00:00

  • Differences in polypeptide composition and enzyme activity between cold-stable and cold-labile microtubules and study of microtubule alkaline phosphatase activity.

    abstract::Cold-stable and cold-labile microtubules were prepared by two cycles of assembly and disassembly and two periods of exposure to cold. The cold-labile preparations were shown to contain a higher proportion of a high molecular mass microtubule-associated protein (MAP 2) than cold-stable preparations. The cold-stable pre...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Hesketh JE

    更新日期:1984-04-24 00:00:00

  • Ceramide generated by acidic sphingomyelinase contributes to tumor necrosis factor-alpha-mediated apoptosis in human colon HT-29 cells through glycosphingolipids formation. Possible role of ganglioside GD3.

    abstract::In the present study we assessed the contribution of acidic sphingomyelinase (ASMase), a ceramide generating enzyme, in tumor necrosis factor (TNF)-mediated apoptosis in human colon HT-29 cells. TNF induced apoptosis in HT-29 cells in a time- and dose-dependent fashion. Downregulation of the active endogenous ASMase f...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Colell A,Morales A,Fernández-Checa JC,García-Ruiz C

    更新日期:2002-08-28 00:00:00

  • Ceramide-1-phosphate: a novel regulator of cell activation.

    abstract::Ceramide-1-phosphate (CER-1-P) is emerging as a novel bioactive sphingolipid. It is formed by phosphorylation of ceramide catalyzed by ceramide kinase, and has been implicated in different cellular processes. Cer-1-P is mitogenic for fibroblasts, blocks apoptosis in macrophages, controls phagocytosis in neutrophils, a...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审


    authors: Gómez-Muñoz A

    更新日期:2004-03-26 00:00:00

  • Pyroglutamate stimulates Na+ -dependent glutamate transport across the blood-brain barrier.

    abstract::Regulation of Na(+)-dependent glutamate transport was studied in isolated luminal and abluminal plasma membranes derived from the bovine blood-brain barrier. Abluminal membranes have Na(+)-dependent glutamate transporters while luminal membranes have facilitative transporters. This organization allows glutamate to be ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Hawkins RA,Simpson IA,Mokashi A,Viña JR

    更新日期:2006-08-07 00:00:00

  • Purification of protein phosphatase from hen oviduct.

    abstract::Two protein phosphatases of 103 and 29 kDa as determined by gel filtration, were purified from hen oviducts. The 103 -kDa phosphatase was purified 7300-fold to near homogeneity and dissociated into two polypeptides in the presence of SDS. Molecular masses of these polypeptides were estimated to be 60 and 38 kDa by SDS...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Kanayama K,Wada K,Negami A,Yamamura H,Tanabe T

    更新日期:1985-05-06 00:00:00

  • Epigallocatechin-3-gallate protects motor neurons and regulates glutamate level.

    abstract::Epigallocatechin-3-gallate (EGCG) is a major component of green tea polyphenols which displays potential properties of anticancer and neuroprotection. Here we show that protection of motor neuron by EGCG is associated with regulating glutamate level in organotypic culture of rat spinal cord. In this model, EGCG blocke...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Yu J,Jia Y,Guo Y,Chang G,Duan W,Sun M,Li B,Li C

    更新日期:2010-07-02 00:00:00

  • Cloning and high-level expression of a chloroperoxidase gene from Pseudomonas pyrrocinia in Escherichia coli.

    abstract::A chloroperoxidase gene from Pseudomonas pyrrocinia was cloned into Escherichia coli using the cosmid vector pJB8. The gene coding for the chloroperoxidase could be localized to a 1.5 kb fragment of DNA which was subcloned into the high-copy-number plasmid pUC18. In one subclone increased halogenating activity could b...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Wolfframm C,van Pée KH,Lingens F

    更新日期:1988-10-10 00:00:00

  • The effect of glycosyl inositol-phosphate on cAMP production in isolated rat fat-cells is transduced by a pertussis toxin sensitive G-protein.

    abstract::In fat-cells, insulin increases synergistically the inhibitory effects of adenosine and prostaglandin E2 on adenylyl cyclase activity. When the endogenous production of these feedback inhibitors is suppressed, insulin is no more active in conditions where glycosyl inositol-phosphate which is a putative mediator of its...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Martiny L,Dib K,Haye B,Corrèze C,Jacquemin C,Lambert B

    更新日期:1991-07-29 00:00:00

  • Two new structured intermediates in the oxidative folding of RNase A.

    abstract::Two new three-disulfide intermediates have been found to be populated in the oxidative folding pathway of bovine pancreatic ribonuclease A at a low temperature (15 degrees C). These intermediates, des-[26-84] and des-[58-110], possess all but one of the four native disulfide bonds and have a stable tertiary structure,...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Welker E,Narayan M,Volles MJ,Scheraga HA

    更新日期:1999-11-05 00:00:00

  • Coupling of viral membrane proteins to phosphatidylinositide signalling system.

    abstract::C6 rat glioma cells persistently infected with subacute sclerosing panencephalitis virus (C6/SSPE) were treated with measles antiserum and purified anti-measles IgG. This stimulated phosphoinositide breakdown and an increase in inositol phosphates. In uninfected C6 cells, however, only fetal calf serum (FCS), but not ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Weinmann-Dorsch C,Koschel K

    更新日期:1989-04-24 00:00:00

  • The oxidant-responsive diaphorase of Rhodobacter capsulatus is a ferredoxin (flavodoxin)-NADP(H) reductase.

    abstract::Challenge of Rhodobacter capsulatus cells with the superoxide propagator methyl viologen resulted in the induction of a diaphorase activity identified as a member of the ferredoxin (flavodoxin)-(reduced) nicotinamide adenine dinucleotide phosphate (NADP(H)) reductase (FPR) family by N-terminal sequencing. The gene cod...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Bittel C,Tabares LC,Armesto M,Carrillo N,Cortez N

    更新日期:2003-10-23 00:00:00

  • Chemical chronobiology: Toward drugs manipulating time.

    abstract::Circadian clocks are endogenous timing systems orchestrating the daily regulation of a huge variety of physiological, metabolic and behavioral processes. These clocks are important for health - in mammals, their disruption leads to a diverse number of pathologies. While genetic and biochemical approaches largely uncov...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审


    authors: Wallach T,Kramer A

    更新日期:2015-06-22 00:00:00

  • Temperature effects on the presteady-state and transport-associated currents of GABA cotransporter rGAT1.

    abstract::The effects of temperature on the gamma-aminobutyric acid (GABA) uptake and on the presteady-state and transport-associated currents of the GABA cotransporter, rat gamma-aminobutyric acid transporter 1 (rGAT1), have been studied using heterologous oocyte expression and voltage-clamp. Increasing temperature from 15 to ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Binda F,Bossi E,Giovannardi S,Forlani G,Peres A

    更新日期:2002-02-13 00:00:00

  • Zinc increases phorbol ester receptors in intact B-cells, neutrophil polymorphs and platelets.

    abstract::In the presence of pyrithione, which was used as a Zn2+ ionophore, Zn2+ (10-100 microM) increased phorbol ester binding by intact B-CLL cells in a dose-dependent fashion. Zn pyrithione increased 2-fold the number of phorbol ester receptors in B-cells (0.74 to 1.4 pmol/10(6) cells), neutrophil polymorphs (0.2 to 0.51 p...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Forbes IJ,Zalewski PD,Hurst NP,Giannakis C,Whitehouse MW

    更新日期:1989-04-24 00:00:00

  • c-Flip(L) is expressed in undifferentiated mouse male germ cells.

    abstract::Apoptosis represents a fundamental process during fetal/post-natal testis development. Therefore pro- and anti-apoptotic proteins are essential to regulate testis physiology. c-Flip(L) is a known inhibitor of caspase 8/10 activity; in this study its perinatal expression in mouse male germ cells was investigated. In te...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Giampietri C,Petrungaro S,Coluccia P,Antonangeli F,Paone A,Padula F,De Cesaris P,Ziparo E,Filippini A

    更新日期:2006-11-13 00:00:00

  • Identification of Lys190 as the primary binding site for pyridoxal 5'-phosphate in human serum albumin.

    abstract::The covalent binding of pyridoxal 5'-phosphate (PLP) to human serum albumin (HSA) is important in the regulation of PLP metabolism. In plasma, PLP is bound to HSA at a single high-affinity and at two or more nonspecific sites. To characterize the primary PLP binding site, HSA was incubated with [3H] PLP, and the Schif...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Bohney JP,Fonda ML,Feldhoff RC

    更新日期:1992-02-24 00:00:00

  • Distinct phospholipase C-regulated signalling pathways in Swiss 3T3 fibroblasts induce the rapid generation of the same polyunsaturated diacylglycerols.

    abstract::Prostaglandin F2alpha, platelet-derived growth factor (PDGF) and calcium ionophore A23187 stimulated the rapid (within 25 s) generation of polyunsaturated 1,2-diacylglycerol (DAG) species, in particular 18:0/20:3n-9, 18:0/20:4n-6 and 18:0/20:5n-3, in Swiss 3T3 fibroblasts. This was followed by a second sustained phase...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Pettitt TR,Wakelam MJ

    更新日期:1998-05-15 00:00:00

  • [Isolation of a new keto-acid, produced from epsilon-N-trimethyllysine by Neurospora crassa].

    abstract::A new epsilon-N-trimethyllysine metabolite has been isolated from the mycelium of Neurospora crassa. The labelled compound produced from incubations in vivo and in vitro from epsilon-N-trimethyl ([14-C]H3)L-lysine has been identified as 2-keto-epsilon-N-trimethyl-hexanoic acid by reducing its 2,4-dinitrophenyl hydrazo...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Villanueva VR,Lederer E

    更新日期:1975-04-01 00:00:00

  • Efficient CD4 binding and immunosuppressive properties of the 13B8.2 monoclonal antibody are displayed by its CDR-H1-derived peptide CB1.

    abstract::A systematic exploration of the V(H)2/V(kappa)12-13 variable domains of the anti-CD4 monoclonal antibody (mAb) 13B8.2 was performed by the Spot method to screen for paratope-derived peptides (PDPs) demonstrating CD4 binding ability. Nine peptides, named CB1 to CB9, were identified, synthesized in a cyclic and soluble ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Bès C,Briant-Longuet L,Cerruti M,De Berardinis P,Devauchelle G,Devaux C,Granier C,Chardès T

    更新日期:2001-11-09 00:00:00

  • Asymmetric dimethylarginine confers the communication between endothelial and smooth muscle cells and leads to VSMC migration through p38 and ERK1/2 signaling cascade.

    abstract::Communication between endothelial and smooth muscle cells (SMCs) contributes to atherosclerosis induced by atherogenic factors, such as oxide LDL. Asymmetric dimethylarginine (ADMA), a newly found cardiovascular risk factor, accumulates in the culture medium of oxide LDL (oxLDL)-treated endothelial cells and positivel...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Sun L,Zhang T,Yu X,Xin W,Lan X,Zhang D,Huang C,Du G

    更新日期:2011-09-02 00:00:00

  • Inhibitors for distinction of three types of human glutathione transferase.

    abstract::A set of inhibitors that are useful for distinction of three types of human cytosolic glutathione transferase is presented. The near-neutral transferase is inhibited most effectively by Cibacron blue (I50 = 0.05 microM), the acidic transferase by Cibacron blue (I50 = 0.5 microM), and the basic transferase by tributylt...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Tahir MK,Guthenberg C,Mannervik B

    更新日期:1985-02-25 00:00:00

  • Ubiquitylation of DNA polymerase λ.

    abstract::DNA polymerase (pol) λ, one of the 15 cellular pols, belongs to the X family. It is a small 575 amino-acid protein containing a polymerase, a dRP-lyase, a proline/serine rich and a BRCT domain. Pol λ shows various enzymatic activities including DNA polymerization, terminal transferase and dRP-lyase. It has been implic...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审


    authors: Markkanen E,van Loon B,Ferrari E,Hübscher U

    更新日期:2011-09-16 00:00:00

  • The transcriptional activator GaaR of Aspergillus niger is required for release and utilization of d-galacturonic acid from pectin.

    abstract::We identified the d-galacturonic acid (GA)-responsive transcriptional activator GaaR of the saprotrophic fungus, Aspergillus niger, which was found to be essential for growth on GA and polygalacturonic acid (PGA). Growth of the ΔgaaR strain was reduced on complex pectins. Genome-wide expression analysis showed that Ga...

    journal_title:FEBS letters

    pub_type: 信件


    authors: Alazi E,Niu J,Kowalczyk JE,Peng M,Aguilar Pontes MV,van Kan JA,Visser J,de Vries RP,Ram AF

    更新日期:2016-06-01 00:00:00

  • Polysome-ribosome distribution in isogenic RC(str) and RC(rel) strains of Escherichia coli.

    abstract::Study of the relative proportions of ribosomes and polysomes released by a standardized lysing procedure from isogenic RC(str) and RC(rel) strains of Escherichia coli shows that a 20-min period of amino acid starvation of RC(str) bacteria reduces the fraction of ribosomes recovered in polysomes to about 60% of its val...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Matzura H

    更新日期:1970-01-15 00:00:00

  • Assignment of the Fe-N epsilon (His) stretching mode in the resonance Raman spectra of a monomeric insect cyanomethaemoglobin.

    abstract::Resonance Raman (RR) spectra of the monomeric cyanomethaemoglobin CTT III from insect larvae of Chironomus thummi thummi are shown for the range of 200-550 cm-1. By iron and cyanide isotope exchange a line varying between 307 cm-1 for 57Fe-13C15N and 311 cm-1 for 54Fe-12C14N, has been assigned to the Fe-N epsilon stre...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Kerr EA,Yu NT,Gersonde K

    更新日期:1984-12-03 00:00:00

  • Streptococci and the complement system: interplay during infection, inflammation and autoimmunity.

    abstract::Streptococci are a broad group of Gram-positive bacteria. This genus includes various human pathogens causing significant morbidity and mortality. Two of the most important human pathogens are Streptococcus pneumoniae (pneumococcus) and Streptococcus pyogenes (group A streptococcus or GAS). Streptococcal pathogens hav...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审


    authors: Syed S,Viazmina L,Mager R,Meri S,Haapasalo K

    更新日期:2020-08-01 00:00:00