Na+, K+-specific inhibition of protein and peptide hydrolyses by proteasomes from human hepatoma tissues.


:Proteasomes were purified from human hepatoma tissues, and their sensitivities to Na+ and K+ were examined. At concentrations of 10 mM or more, these cations were found to inhibit completely polylysine-activated casein degradation by the purified proteasomes. They also strongly inhibited the hydrolyses of peptides, although to a lesser extent. On the other hand, they reversed the inhibitory and stimulatory effects of polylysine on the hydrolyses of Suc-Leu-Tyr-AMC and Cbz-Ala-Arg-Arg-MNA, respectively. These results suggest that Na+ and/or K+ may be involved in the regulation of intracellular protein breakdown by controlling the multicatalytic activity of proteasomes.




FEBS letters


Seol JH,Park SC,Ha DB,Chung CH,Tanaka K,Ichihara A




Has Abstract


1989-04-24 00:00:00














  • Fertilization of Xenopus eggs imposes a complete translational arrest of mRNAs containing 3'UUAUUUAU elements.

    abstract::The early embryonic development of Xenopus is mainly governed by post-transcriptional regulations until the mid-blastula transition. In this report, we present evidence demonstrating that fertilization of Xenopus eggs triggers a complete translational arrest of mRNAs containing UA-rich elements in their 3'-untranslate...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Marinx O,Bertrand S,Karsenti E,Huez G,Kruys V

    更新日期:1994-05-30 00:00:00

  • Prooxidant iron and copper, with ferroxidase and xanthine oxidase activities in human atherosclerotic material.

    abstract::Low density lipoproteins are highly sensitive to oxidation by copper salts, and such peroxidation is accompanied by macrophage scavenger receptor recognition. This study shows that fresh human atherosclerotic material (aneurysms and endarterectomies) can contain detectable amounts of redox active iron and copper that ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Swain J,Gutteridge JM

    更新日期:1995-07-24 00:00:00

  • Studies on the interaction of the dye, stains-all, with individual calcium-binding domains of calmodulin.

    abstract::We show that the calcium-mimic dye, Stains-all, is a convenient probe to study the structural features of the individual calcium-binding sites of calmodulin (CaM) and related calcium-binding proteins (CaBP). These peptides bind the dye in their calcium-binding sites, and induce a circular dichroism (CD) band in the bo...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Sharma Y,Gopalakrishna A,Balasubramanian D,Fairwell T,Krishna G

    更新日期:1993-07-12 00:00:00

  • Peculiarities of immunoglobulin gene structures as a basis for somatic mutation emergence.

    abstract::Using the method of contextual analysis, we have studied the collection of somatic mutations in immunoglobulin genes. It has been found that the emergence of somatic mutations can be based on the reparation of complementarity violations in the heteroduplexes corresponding to complementary palindromes or direct repeats...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Kolchanov NA,Solovyov VV,Rogozin IB

    更新日期:1987-04-06 00:00:00

  • Recognition of an antiparallel beta-sheet structure of human epidermal growth factor by its receptor. Site-directed mutagenesis studies of Ala-30 and Asn-32.

    abstract::The Ala-30 and Asn-32 residues involved in the major antiparallel beta-sheet structure of human epidermal growth factor (hEGF) were substituted with various amino acid residues, and the receptor-binding affinities of the nine variant hEGFs were determined by the use of human KB cells. The Ala-30----Arg, Ala-30----His ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Koide H,Muto Y,Kasai H,Hoshi K,Takusari H,Kohri K,Takahashi S,Sasaki T,Tsukumo K,Miyake T

    更新日期:1992-05-04 00:00:00

  • RNA/DNA hybrid duplexes with identical nearest-neighbor base-pairs have identical stability.

    abstract::Energetic behaviors of eight pairs of RNA/DNA hybrid duplexes with identical nearest neighbors have been investigated by UV melting analysis. In the pairs with identical nearest-neighbor pairs, the melting curve traces at the same strand concentration were very similar. The average difference in stabilization energy o...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Sugimoto N,Katoh M,Nakano S,Ohmichi T,Sasaki M

    更新日期:1994-10-31 00:00:00

  • Thymidylate synthetase-positive and -negative murine mammary FM3A carcinoma cells as a useful system for detecting thymidylate synthetase inhibitors.

    abstract::The murine mammary FM3A/O and the thymidylate (dTMP) synthetase-deficient FM3A/TS- carcinoma cell lines can be considered as a novel and useful test system for the detection of nucleoside analogues which are directly aimed at the thymidylate synthetase. These compounds should be inhibitory for FM3A/O but not for FM3A/...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Balzarini J,de Clercq E,Ayusawa D,Seno T

    更新日期:1984-07-23 00:00:00

  • NAD(P)H- and superoxide-dependent nitric oxide degradation by rat liver mitochondria.

    abstract::Mitochondria consume nitric oxide (NO) mainly through reaction with superoxide anion (O(2)(-)). Here, we analyzed the O(2)(-) sources for NO degradation by isolated rat liver mitochondria. Electron leakage from complex III and reverse electron transport to complex I accounted for O(2)(-)-dependent NO degradation by mi...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Oliveira HC,Saviani EE,Salgado I

    更新日期:2009-07-07 00:00:00

  • Posttranscriptional regulation of c-myc RNA during early development of Xenopus laevis.

    abstract::The remarkable stability of c-myc during oogenesis contrasts with its degradation during the early developmental period in Xenopus laevis. Three evolutionary conserved motifs found in the 3'-untranslated region of Xenopus c-myc RNAs have been analyzed for a possible role in c-myc RNA degradation. No specific degradati...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Tchang F,Vriz S,Méchali M

    更新日期:1991-10-21 00:00:00

  • The SUPERMAN protein is an active repressor whose carboxy-terminal repression domain is required for the development of normal flowers.

    abstract::SUPERMAN was identified as a putative regulator of transcription that acts in floral development, but its function remains to be clarified. We demonstrate here that SUPERMAN is an active repressor whose repression domain is located in the carboxy-terminal region. Ectopic expression of SUPERMAN that lacked the repressi...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Hiratsu K,Ohta M,Matsui K,Ohme-Takagi M

    更新日期:2002-03-13 00:00:00

  • Overexpression of the glucose transporter GLUT4 in adipose cells interferes with insulin-stimulated translocation.

    abstract::In adipose cells, insulin induces the translocation of GLUT4 by stimulating their exocytosis from a basal intracellular compartment to the plasma membrane. Increasing overexpression of a hemagglutinin (HA) epitope-tagged GLUT4 in rat adipose cells results in a roughly proportional increase in cell surface HA-GLUT4 lev...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Al-Hasani H,Yver DR,Cushman SW

    更新日期:1999-10-29 00:00:00

  • Endogenous small RNAs and antibacterial immunity in plants.

    abstract::Small RNAs are non-coding regulatory RNA molecules that control gene expression by mediating mRNA degradation, translational inhibition, or chromatin modification. Virus-derived small RNAs induce silencing of viral RNAs and are essential for antiviral defense in both animal and plant systems. The role of host endogeno...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审


    authors: Jin H

    更新日期:2008-08-06 00:00:00

  • Analysis of PI (phosphatidylinositol)-anchoring antigens in a patient of paroxysmal nocturnal hemoglobinuria (PNH) reveals deficiency of 1F5 antigen (CD59), a new complement-regulatory factor.

    abstract::FACS analysis together with PIPLC treatment was applied to PI-anchoring antigens such as DAF (decay-accerelating factor, CD55), 1F5 antigen (CD59), CD14 and CD16 on the cell surfaces of blood cells from a normal adult and a male patient with paroxysmal nocturnal hemoglubinuria (PNH). Through the extensive analysis, th...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Taguchi R,Funahashi Y,Ikezawa H,Nakashima I

    更新日期:1990-02-12 00:00:00

  • Single-chain precursor of renal gamma-glutamyl transpeptidase.

    abstract::The two subunits of gamma-glutamyl transpeptidase (EC are derived from a single-chain glycosylated precursor. A small fraction of the propeptide survives proteolytic processing in the rat kidney and has been purified by an immunoaffinity technique. The propeptide contains determinants for both the subunits an...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Tate SS

    更新日期:1986-01-01 00:00:00

  • Activation of an Asp-124-->Asn mutant of haloalkane dehalogenase by hydrolytic deamidation of asparagine.

    abstract::Haloalkane dehalogenase hydrolyses various 1-halo-n-alkanes to the corresponding alcohols by covalent catalysis with formation of an alkyl-enzyme intermediate. The carboxylate function of the nucleophilic aspartate (Asp-124) that displaces the halogen during formation of the intermediate was changed to an amide by sit...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Pries F,Kingma J,Janssen DB

    更新日期:1995-01-23 00:00:00

  • Disruption of actin microfilaments by cytochalasin D leads to activation of p53.

    abstract::Activation of p53 plays a central role in the cell's response to various stress signals. We investigated whether p53 is activated upon disruption of actin microfilaments, caused by cytochalasin D (CD). We show that treatment with CD leads to accumulation of p53 in the cells and activation of p53-dependent transcriptio...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Rubtsova SN,Kondratov RV,Kopnin PB,Chumakov PM,Kopnin BP,Vasiliev JM

    更新日期:1998-07-03 00:00:00

  • APP RNA splicing is not affected by differentiation of neurons and glia in culture.

    abstract::Amyloid precursor protein (APP) gene expression was investigated in primary cultures of neurons, astrocytes, microglial cells and oligodendrocytes. Neurons from various rat brain regions, as well as oligodendrocytes, contained RNA encoding APP695, while astrocytes and microglial cells expressed high levels of RNAs for...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Gaul G,Dutly F,Frei K,Foguet M,Lübbert H

    更新日期:1992-08-03 00:00:00

  • Ascorbic acid can either increase or decrease low density lipoprotein modification.

    abstract::In freshly prepared low density lipoprotein (LDL), ascorbate inhibited LDL oxidation by macrophages at the higher concentrations tested (60-100 microM). In contrast, with LDL that had been allowed to autoxidise in the refrigerator (3 degrees C) for at least 10 weeks after isolation (mildly oxidised or minimally-modifi...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Stait SE,Leake DS

    更新日期:1994-03-21 00:00:00

  • Free sphingobases induce RBOHD-dependent reactive oxygen species production in Arabidopsis leaves.

    abstract::Sphingolipids are implied in several regulatory processes, including cell death. Levels of the free sphingobase t18:0 (phytosphingosine) increase in Arabidopsis in response to the bacterial pathogen Pseudomonas syringae. To gain information on sphingobase-induced signaling, we determined kinetics of leaf reactive oxyg...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Peer M,Bach M,Mueller MJ,Waller F

    更新日期:2011-10-03 00:00:00

  • Redox properties of tyrosine and related molecules.

    abstract::Redox reactions of tyrosine play key roles in many biological processes, including water oxidation and DNA synthesis. We first review the redox properties of tyrosine (and other phenols) in small molecules and related polypeptides, then report work on (H20)/(Y48)-modified Pseudomonas aeruginosa azurin. The crystal str...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审


    authors: Warren JJ,Winkler JR,Gray HB

    更新日期:2012-03-09 00:00:00

  • Multicatalytic and 26 S ubiquitin/ATP-stimulated proteases in maturing rabbit red blood cells.

    abstract::Rabbit red blood cells of various ages were separated on Percoll gradients and the activities of two large cytosolic proteases were measured. Both the multicatalytic protease (MCP), assayed by hydrolysis of fluorigenic peptides, and the 26 S ubiquitin/ATP-stimulated protease, assayed by degradation of ubiquitin-lysozy...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Di Cola D,Pratt G,Rechsteiner M

    更新日期:1991-03-11 00:00:00

  • Alternate quinone coupling in a new class of succinate dehydrogenase may potentiate mycobacterial respiratory control.

    abstract::There is a paucity of information on the unique components that pathogens use to form respiratory chains. It is not known why mycobacteria encode multiple succinate dehydrogenases (SDHs) to perform menaquinone-linked succinate oxidation, a thermodynamically unfavorable reaction (ΔG° = +21 kJ·mol-1 ). In other bacteria...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Hards K,Rodriguez SM,Cairns C,Cook GM

    更新日期:2019-03-01 00:00:00

  • Solution structure and p43 binding of the p38 leucine zipper motif: coiled-coil interactions mediate the association between p38 and p43.

    abstract::p38, which has been suggested to be a scaffold protein for the assembly of a macromolecular tRNA synthetase complex, contains a leucine zipper-like motif. To understand the importance of the leucine zipper-like motif of p38 (p38LZ) in macromolecular assembly, the p38LZ solution structure was investigated by circular d...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Ahn HC,Kim S,Lee BJ

    更新日期:2003-05-08 00:00:00

  • Cyclic AMP-dependent protein kinase regulates 9G8-mediated alternative splicing of tau exon 10.

    abstract::Alternative splicing of tau exon 10 generates tau isoforms with three or four microtubule-binding repeats, named 3R- or 4R-tau. Normal adult human brain expresses equal levels of them. Imbalance of 3R-tau and 4R-tau associates with several tauopathies. Splicing factor 9G8 suppresses tau exon 10 inclusion and its funct...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Gu J,Shi J,Wu S,Jin N,Qian W,Zhou J,Iqbal IG,Iqbal K,Gong CX,Liu F

    更新日期:2012-07-30 00:00:00

  • Selective inhibition of divergent seryl-tRNA synthetases by serine analogues.

    abstract::Seryl-tRNA synthetases (SerRSs) fall into two distinct evolutionary groups of enzymes, bacterial and methanogenic. These two types of SerRSs display only minimal sequence similarity, primarily within the class II conserved motifs, and possess distinct modes of tRNA(Ser) recognition. In order to determine whether the t...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Ahel D,Slade D,Mocibob M,Söll D,Weygand-Durasevic I

    更新日期:2005-08-15 00:00:00

  • Molecular cloning of the Na,K-ATPase alpha-subunit in developing brine shrimp and sequence comparison with higher organisms.

    abstract::We report here the molecular cloning, nucleotide sequence, and predicted amino acid sequence of an alpha-subunit of the developmentally useful model, Artemia. The amino acid sequence shows divergence from that of mammals, birds, Torpedo, and Drosophila. However, regions in the putative ATP binding and transmembrane do...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Baxter-Lowe LA,Guo JZ,Bergstrom EE,Hokin LE

    更新日期:1989-10-23 00:00:00

  • The binding interface of kindlin-2 and ILK involves Asp344/Asp352/Thr356 in kindlin-2 and Arg243/Arg334 in ILK.

    abstract::Focal adhesion (FA) proteins, kindlin-2 and integrin-linked kinase (ILK), regulate cell adhesion and migration. ILK interacts with and promotes kindlin-2 targeting to FAs. Leu353 and Leu357 in kindlin-2 have been reported to be important for the interaction between kindlin-2 and ILK. However, the binding interface bet...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Guan SY,Chng CP,Ong LT,Tan HF,Alex Law SK,Tan SM

    更新日期:2018-01-01 00:00:00

  • Up- and downregulated genes in muscles that undergo developmentally programmed cell death in the insect Manduca sexta.

    abstract::This study was designed to investigate changes in gene expression associated with stage-specific programmed cell death (PCD) in intersegmental muscles (ISMs) of the moth, Manduca sexta. The technique of differential display reverse transcription PCR was applied to compare mRNA levels before and after the onset of PCD ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Löw P,Talián GC,Sass M

    更新日期:2005-09-12 00:00:00

  • Determination of fast proton exchange rates of biomolecules by NMR using water selective diffusion experiments.

    abstract::We present a new water selective pulse sequence allowing rapid determination of exchange rates of labile protons on the millisecond time scale. Using diffusion measurements, exchange rates of resolved protons can be determined without prior knowledge of relaxation parameters in a short overnight experiment. The use of...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Böckmann A,Guittet E

    更新日期:1997-11-24 00:00:00

  • Plant polyphenols inhibit VacA, a toxin secreted by the gastric pathogen Helicobacter pylori.

    abstract::VacA is a major virulence factor of the widespread stomach-dwelling bacterium Helicobacter pylori. It causes cell vacuolation and tissue damage by forming anion-selective, urea-permeable channels in plasma and endosomal membranes. We report that several flavone derivatives and other polyphenols present in vegetables a...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Tombola F,Campello S,De Luca L,Ruggiero P,Del Giudice G,Papini E,Zoratti M

    更新日期:2003-05-22 00:00:00