Na+, K+-specific inhibition of protein and peptide hydrolyses by proteasomes from human hepatoma tissues.

Abstract:

:Proteasomes were purified from human hepatoma tissues, and their sensitivities to Na+ and K+ were examined. At concentrations of 10 mM or more, these cations were found to inhibit completely polylysine-activated casein degradation by the purified proteasomes. They also strongly inhibited the hydrolyses of peptides, although to a lesser extent. On the other hand, they reversed the inhibitory and stimulatory effects of polylysine on the hydrolyses of Suc-Leu-Tyr-AMC and Cbz-Ala-Arg-Arg-MNA, respectively. These results suggest that Na+ and/or K+ may be involved in the regulation of intracellular protein breakdown by controlling the multicatalytic activity of proteasomes.

journal_name

FEBS Lett

journal_title

FEBS letters

authors

Seol JH,Park SC,Ha DB,Chung CH,Tanaka K,Ichihara A

doi

10.1016/0014-5793(89)81333-x

subject

Has Abstract

pub_date

1989-04-24 00:00:00

pages

197-200

issue

2

eissn

0014-5793

issn

1873-3468

pii

0014-5793(89)81333-X

journal_volume

247

pub_type

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