Na+, K+-specific inhibition of protein and peptide hydrolyses by proteasomes from human hepatoma tissues.

abstract：:Treatment of 125I-labelled high-density lipoprotein ([125I]HDL3) with monospecific polyclonal antibodies against apolipoproteins A-I and A-II resulted in a dose-dependent inhibition of the [125I]HDL3 binding to isolated human small intestine epithelial cells by 25% and 50%, respectively. Both antibodies also inhibited...

journal_title：FEBS letters

authors： Sviridov DD,Ehnholm C,Tenkanen H,Pavlov MYu,Safonova IG,Repin VS

更新日期：1992-06-01 00:00:00

abstract：:Plasma high density lipoprotein (HDL)-cholesterol levels are inversely correlated with the incidence of cardiovascular diseases. HDL is mainly assembled in the liver through the ATP-binding cassette transporter (ABCA1) pathway. In humans, plasma HDL-cholesterol levels are positively correlated with plasma adiponectin ...

journal_title：FEBS letters

authors： Oku H,Matsuura F,Koseki M,Sandoval JC,Yuasa-Kawase M,Tsubakio-Yamamoto K,Masuda D,Maeda N,Ohama T,Ishigami M,Nishida M,Hirano K,Kihara S,Hori M,Shimomura I,Yamashita S

更新日期：2007-10-30 00:00:00

abstract：:A peroxidase with stability, chromatographic and immunoreactive properties similar to that of bovine lactoperoxidase has been partly purified from human colostrum. Hydrophobic interaction chromatography on Phenyl-Sepharose C1-4B gave a 10-fold purification with an apparent recovery of about 45%. The enzyme was quantit...

journal_title：FEBS letters

authors： Langbakk B,Flatmark T

更新日期：1984-09-03 00:00:00

abstract：:Interactions between Trypanosoma cruzi and the extracellular matrix play an important role in cellular invasion. Here we show that T. cruzi increases the levels of thrombospondin-1 (TSP-1) expression in host cells during early infection. Stable RNA interference of host cell TSP-1 knocks down the levels of TSP-1 transc...

journal_title：FEBS letters

authors： Simmons KJ,Nde PN,Kleshchenko YY,Lima MF,Villalta F

更新日期：2006-04-17 00:00:00

abstract：:Cold-stable and cold-labile microtubules were prepared by two cycles of assembly and disassembly and two periods of exposure to cold. The cold-labile preparations were shown to contain a higher proportion of a high molecular mass microtubule-associated protein (MAP 2) than cold-stable preparations. The cold-stable pre...

journal_title：FEBS letters

authors： Hesketh JE

更新日期：1984-04-24 00:00:00

abstract：:In the present study we assessed the contribution of acidic sphingomyelinase (ASMase), a ceramide generating enzyme, in tumor necrosis factor (TNF)-mediated apoptosis in human colon HT-29 cells. TNF induced apoptosis in HT-29 cells in a time- and dose-dependent fashion. Downregulation of the active endogenous ASMase f...

journal_title：FEBS letters

authors： Colell A,Morales A,Fernández-Checa JC,García-Ruiz C

更新日期：2002-08-28 00:00:00

abstract：:Ceramide-1-phosphate (CER-1-P) is emerging as a novel bioactive sphingolipid. It is formed by phosphorylation of ceramide catalyzed by ceramide kinase, and has been implicated in different cellular processes. Cer-1-P is mitogenic for fibroblasts, blocks apoptosis in macrophages, controls phagocytosis in neutrophils, a...

journal_title：FEBS letters

authors： Gómez-Muñoz A

更新日期：2004-03-26 00:00:00

abstract：:Regulation of Na(+)-dependent glutamate transport was studied in isolated luminal and abluminal plasma membranes derived from the bovine blood-brain barrier. Abluminal membranes have Na(+)-dependent glutamate transporters while luminal membranes have facilitative transporters. This organization allows glutamate to be ...

journal_title：FEBS letters

authors： Hawkins RA,Simpson IA,Mokashi A,Viña JR

更新日期：2006-08-07 00:00:00

abstract：:Two protein phosphatases of 103 and 29 kDa as determined by gel filtration, were purified from hen oviducts. The 103 -kDa phosphatase was purified 7300-fold to near homogeneity and dissociated into two polypeptides in the presence of SDS. Molecular masses of these polypeptides were estimated to be 60 and 38 kDa by SDS...

journal_title：FEBS letters

authors： Kanayama K,Wada K,Negami A,Yamamura H,Tanabe T

更新日期：1985-05-06 00:00:00

abstract：:Epigallocatechin-3-gallate (EGCG) is a major component of green tea polyphenols which displays potential properties of anticancer and neuroprotection. Here we show that protection of motor neuron by EGCG is associated with regulating glutamate level in organotypic culture of rat spinal cord. In this model, EGCG blocke...

journal_title：FEBS letters

authors： Yu J,Jia Y,Guo Y,Chang G,Duan W,Sun M,Li B,Li C

更新日期：2010-07-02 00:00:00

abstract：:A chloroperoxidase gene from Pseudomonas pyrrocinia was cloned into Escherichia coli using the cosmid vector pJB8. The gene coding for the chloroperoxidase could be localized to a 1.5 kb fragment of DNA which was subcloned into the high-copy-number plasmid pUC18. In one subclone increased halogenating activity could b...

journal_title：FEBS letters

authors： Wolfframm C,van Pée KH,Lingens F

更新日期：1988-10-10 00:00:00

abstract：:In fat-cells, insulin increases synergistically the inhibitory effects of adenosine and prostaglandin E2 on adenylyl cyclase activity. When the endogenous production of these feedback inhibitors is suppressed, insulin is no more active in conditions where glycosyl inositol-phosphate which is a putative mediator of its...

journal_title：FEBS letters

authors： Martiny L,Dib K,Haye B,Corrèze C,Jacquemin C,Lambert B

更新日期：1991-07-29 00:00:00

abstract：:Two new three-disulfide intermediates have been found to be populated in the oxidative folding pathway of bovine pancreatic ribonuclease A at a low temperature (15 degrees C). These intermediates, des-[26-84] and des-[58-110], possess all but one of the four native disulfide bonds and have a stable tertiary structure,...

journal_title：FEBS letters

authors： Welker E,Narayan M,Volles MJ,Scheraga HA

更新日期：1999-11-05 00:00:00

abstract：:C6 rat glioma cells persistently infected with subacute sclerosing panencephalitis virus (C6/SSPE) were treated with measles antiserum and purified anti-measles IgG. This stimulated phosphoinositide breakdown and an increase in inositol phosphates. In uninfected C6 cells, however, only fetal calf serum (FCS), but not ...

journal_title：FEBS letters

authors： Weinmann-Dorsch C,Koschel K

更新日期：1989-04-24 00:00:00

abstract：:Challenge of Rhodobacter capsulatus cells with the superoxide propagator methyl viologen resulted in the induction of a diaphorase activity identified as a member of the ferredoxin (flavodoxin)-(reduced) nicotinamide adenine dinucleotide phosphate (NADP(H)) reductase (FPR) family by N-terminal sequencing. The gene cod...

journal_title：FEBS letters

authors： Bittel C,Tabares LC,Armesto M,Carrillo N,Cortez N

更新日期：2003-10-23 00:00:00

abstract：:Circadian clocks are endogenous timing systems orchestrating the daily regulation of a huge variety of physiological, metabolic and behavioral processes. These clocks are important for health - in mammals, their disruption leads to a diverse number of pathologies. While genetic and biochemical approaches largely uncov...

journal_title：FEBS letters

authors： Wallach T,Kramer A

更新日期：2015-06-22 00:00:00

abstract：:The effects of temperature on the gamma-aminobutyric acid (GABA) uptake and on the presteady-state and transport-associated currents of the GABA cotransporter, rat gamma-aminobutyric acid transporter 1 (rGAT1), have been studied using heterologous oocyte expression and voltage-clamp. Increasing temperature from 15 to ...

journal_title：FEBS letters

authors： Binda F,Bossi E,Giovannardi S,Forlani G,Peres A

更新日期：2002-02-13 00:00:00

abstract：:In the presence of pyrithione, which was used as a Zn2+ ionophore, Zn2+ (10-100 microM) increased phorbol ester binding by intact B-CLL cells in a dose-dependent fashion. Zn pyrithione increased 2-fold the number of phorbol ester receptors in B-cells (0.74 to 1.4 pmol/10(6) cells), neutrophil polymorphs (0.2 to 0.51 p...

journal_title：FEBS letters

authors： Forbes IJ,Zalewski PD,Hurst NP,Giannakis C,Whitehouse MW

更新日期：1989-04-24 00:00:00

abstract：:Apoptosis represents a fundamental process during fetal/post-natal testis development. Therefore pro- and anti-apoptotic proteins are essential to regulate testis physiology. c-Flip(L) is a known inhibitor of caspase 8/10 activity; in this study its perinatal expression in mouse male germ cells was investigated. In te...

journal_title：FEBS letters

authors： Giampietri C,Petrungaro S,Coluccia P,Antonangeli F,Paone A,Padula F,De Cesaris P,Ziparo E,Filippini A

更新日期：2006-11-13 00:00:00

abstract：:The covalent binding of pyridoxal 5'-phosphate (PLP) to human serum albumin (HSA) is important in the regulation of PLP metabolism. In plasma, PLP is bound to HSA at a single high-affinity and at two or more nonspecific sites. To characterize the primary PLP binding site, HSA was incubated with [3H] PLP, and the Schif...

journal_title：FEBS letters

authors： Bohney JP,Fonda ML,Feldhoff RC

更新日期：1992-02-24 00:00:00

abstract：:Prostaglandin F2alpha, platelet-derived growth factor (PDGF) and calcium ionophore A23187 stimulated the rapid (within 25 s) generation of polyunsaturated 1,2-diacylglycerol (DAG) species, in particular 18:0/20:3n-9, 18:0/20:4n-6 and 18:0/20:5n-3, in Swiss 3T3 fibroblasts. This was followed by a second sustained phase...

journal_title：FEBS letters

authors： Pettitt TR,Wakelam MJ

更新日期：1998-05-15 00:00:00

abstract：:A new epsilon-N-trimethyllysine metabolite has been isolated from the mycelium of Neurospora crassa. The labelled compound produced from incubations in vivo and in vitro from epsilon-N-trimethyl ([14-C]H3)L-lysine has been identified as 2-keto-epsilon-N-trimethyl-hexanoic acid by reducing its 2,4-dinitrophenyl hydrazo...

journal_title：FEBS letters

authors： Villanueva VR,Lederer E

更新日期：1975-04-01 00:00:00

abstract：:A systematic exploration of the V(H)2/V(kappa)12-13 variable domains of the anti-CD4 monoclonal antibody (mAb) 13B8.2 was performed by the Spot method to screen for paratope-derived peptides (PDPs) demonstrating CD4 binding ability. Nine peptides, named CB1 to CB9, were identified, synthesized in a cyclic and soluble ...

journal_title：FEBS letters

authors： Bès C,Briant-Longuet L,Cerruti M,De Berardinis P,Devauchelle G,Devaux C,Granier C,Chardès T

更新日期：2001-11-09 00:00:00

abstract：:Communication between endothelial and smooth muscle cells (SMCs) contributes to atherosclerosis induced by atherogenic factors, such as oxide LDL. Asymmetric dimethylarginine (ADMA), a newly found cardiovascular risk factor, accumulates in the culture medium of oxide LDL (oxLDL)-treated endothelial cells and positivel...

journal_title：FEBS letters

authors： Sun L,Zhang T,Yu X,Xin W,Lan X,Zhang D,Huang C,Du G

更新日期：2011-09-02 00:00:00

abstract：:A set of inhibitors that are useful for distinction of three types of human cytosolic glutathione transferase is presented. The near-neutral transferase is inhibited most effectively by Cibacron blue (I50 = 0.05 microM), the acidic transferase by Cibacron blue (I50 = 0.5 microM), and the basic transferase by tributylt...

journal_title：FEBS letters

authors： Tahir MK,Guthenberg C,Mannervik B

更新日期：1985-02-25 00:00:00

abstract：:DNA polymerase (pol) λ, one of the 15 cellular pols, belongs to the X family. It is a small 575 amino-acid protein containing a polymerase, a dRP-lyase, a proline/serine rich and a BRCT domain. Pol λ shows various enzymatic activities including DNA polymerization, terminal transferase and dRP-lyase. It has been implic...

journal_title：FEBS letters

authors： Markkanen E,van Loon B,Ferrari E,Hübscher U

更新日期：2011-09-16 00:00:00

abstract：:We identified the d-galacturonic acid (GA)-responsive transcriptional activator GaaR of the saprotrophic fungus, Aspergillus niger, which was found to be essential for growth on GA and polygalacturonic acid (PGA). Growth of the ΔgaaR strain was reduced on complex pectins. Genome-wide expression analysis showed that Ga...

journal_title：FEBS letters

authors： Alazi E,Niu J,Kowalczyk JE,Peng M,Aguilar Pontes MV,van Kan JA,Visser J,de Vries RP,Ram AF

更新日期：2016-06-01 00:00:00

abstract：:Study of the relative proportions of ribosomes and polysomes released by a standardized lysing procedure from isogenic RC(str) and RC(rel) strains of Escherichia coli shows that a 20-min period of amino acid starvation of RC(str) bacteria reduces the fraction of ribosomes recovered in polysomes to about 60% of its val...

journal_title：FEBS letters

authors： Matzura H

更新日期：1970-01-15 00:00:00

abstract：:Resonance Raman (RR) spectra of the monomeric cyanomethaemoglobin CTT III from insect larvae of Chironomus thummi thummi are shown for the range of 200-550 cm-1. By iron and cyanide isotope exchange a line varying between 307 cm-1 for 57Fe-13C15N and 311 cm-1 for 54Fe-12C14N, has been assigned to the Fe-N epsilon stre...

journal_title：FEBS letters

authors： Kerr EA,Yu NT,Gersonde K

更新日期：1984-12-03 00:00:00

abstract：:Streptococci are a broad group of Gram-positive bacteria. This genus includes various human pathogens causing significant morbidity and mortality. Two of the most important human pathogens are Streptococcus pneumoniae (pneumococcus) and Streptococcus pyogenes (group A streptococcus or GAS). Streptococcal pathogens hav...

journal_title：FEBS letters

authors： Syed S,Viazmina L,Mager R,Meri S,Haapasalo K

更新日期：2020-08-01 00:00:00