Abstract:
:Proteasomes were purified from human hepatoma tissues, and their sensitivities to Na+ and K+ were examined. At concentrations of 10 mM or more, these cations were found to inhibit completely polylysine-activated casein degradation by the purified proteasomes. They also strongly inhibited the hydrolyses of peptides, although to a lesser extent. On the other hand, they reversed the inhibitory and stimulatory effects of polylysine on the hydrolyses of Suc-Leu-Tyr-AMC and Cbz-Ala-Arg-Arg-MNA, respectively. These results suggest that Na+ and/or K+ may be involved in the regulation of intracellular protein breakdown by controlling the multicatalytic activity of proteasomes.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Seol JH,Park SC,Ha DB,Chung CH,Tanaka K,Ichihara Adoi
10.1016/0014-5793(89)81333-xsubject
Has Abstractpub_date
1989-04-24 00:00:00pages
197-200issue
2eissn
0014-5793issn
1873-3468pii
0014-5793(89)81333-Xjournal_volume
247pub_type
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