Heme incorporation into the cytochrome bo3 occurs at a late stage of assembly.


:Respiratory complexes in both prokaryotes and eukaryotes contain multiple co-factors, which are coordinated in defined positions so that they can function as electron wires. Intriguingly, co-factors are usually buried deep within hetero-oligomeric protein complexes and it is not clear when or how they are incorporated. In this study we show that heme is incorporated into the cytochrome bo(3) complex of Escherichia coli at a late stage of assembly. Specifically the apo-form of subunit I (the catalytic subunit) interacts with subunits III and IV before accepting heme. Assembly of subunit II is stalled until heme is incorporated.




FEBS letters


Palombo I,Daley DO




Has Abstract


2012-11-30 00:00:00














  • Ectopic synthesis of high-Mr calcitonin by the BEN lung carcinoma cell line reflects aberrant proteolytic processing.

    abstract::Cloning and nucleotide sequence analysis of the human calcitonin mRNA from the BEN lung carcinoma cell line, a cell line known to secrete high-Mr forms of calcitonin, showed no difference in the coding region at the nucleotide level compared with calcitonin mRNA isolated from medullary thyroid carcinoma which secretes...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Riley JH,Edbrooke MR,Craig RK

    更新日期:1986-03-17 00:00:00

  • SV40 chromatin structure is not essential for viral gene expression.

    abstract::The biological activity and the fate of SV40 DNA (minichromosomes, DNA I, DNA II, DNA III) were tested in culture cells by immunofluorescence staining and blot analysis. Following microinjection of 2-4 circular SV40 molecules (minichromosomes, DNA I, DNA II) into the cytoplasm or the nuclei of monkey and rat cells, T-...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Graessmann A,Bumke-Vogt C,Buschhausen G,Bauer M,Graessmann M

    更新日期:1985-01-01 00:00:00

  • Two distinct classes of rat intestinal mucosal enzymes incorporating putrescine into protein.

    abstract::Tissue-transglutaminase (t-TGase) is a family of calcium-dependent enzymes. A Ca2+-independent soluble enzyme, in addition to t-TGase, capable of incorporating polyamines into proteins was demonstrated in rat intestinal mucosa. The Ca2+-independent enzyme was stimulated 2- to 5-fold by Fe2+ and Co2+ ions but inhibited...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Tsai YH,Lai WF,Wu YW,Johnson LR

    更新日期:1998-09-18 00:00:00

  • Increased expression of vascular endothelial growth factor in placentas of p57(Kip2) null embryos.

    abstract::Placentas of mice lacking p57(Kip2) expression have trophoblastic hyperplasia. To elucidate the mechanism underlying this phenomenon, we studied expression of two angiogenic factors, vascular endothelial growth factor (VEGF) and placenta growth factor (PlGF). Immunohistochemical analysis with anti-VEGF antibodies indi...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Matsuura T,Takahashi K,Nakayama K,Kobayashi T,Choi-Miura NH,Tomita M,Kanayama N

    更新日期:2002-12-18 00:00:00

  • Volume-dependent and NEM-stimulated K+,Cl- transport is elevated in oxygenated SS, SC and CC human red cells.

    abstract::Mechanisms involved in cell volume regulation are important in SS, SC cells as they might be involved in determining the extent of sickling and the generation of dense cells and irreversibly sickled cells. We have studied in these cells the response to cell swelling of the K+,Cl- transporter. We found that Hb SS, SC a...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Canessa M,Spalvins A,Nagel RL

    更新日期:1986-05-05 00:00:00

  • Functional and structural characterisation of a bacterial O-methyltransferase and factors determining regioselectivity.

    abstract::Mg2+ -dependent catechol-O-methyltransferases occur in animals as well as in bacteria, fungi and plants, often with a pronounced selectivity towards one of the substrate's hydroxyl groups. Here, we show that the bacterial MxSafC exhibits excellent regioselectivity for para as well as for meta methylation, depending on...

    journal_title:FEBS letters

    pub_type: 信件


    authors: Siegrist J,Netzer J,Mordhorst S,Karst L,Gerhardt S,Einsle O,Richter M,Andexer JN

    更新日期:2017-01-01 00:00:00

  • Functional argument for the existence of an avian nitric oxide synthase in muscle mitochondria: effect of cold acclimation.

    abstract::We report the first evidence of a mitochondrial NO synthase (mtNOS) in bird skeletal muscle. In vitro, mtNOS activity stimulated by L-arginine reduced intermyofibrillar mitochondrial oxygen uptake and ATP synthesis rates, stimulated endogenous H(2)O(2) generation, but had no effect on oxidative phosphorylation efficie...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Rey B,Roussel D,Teulier L,Eyenga P,Degletagne C,Belouze M,Duchamp C

    更新日期:2011-01-03 00:00:00

  • Caenorhabditis elegans small heat-shock proteins Hsp12.2 and Hsp12.3 form tetramers and have no chaperone-like activity.

    abstract::Four 12.2-12.6 kDa small heat-shock proteins (sHSPs) of Caenorhabditis elegans are the smallest known members of the sHSP family. They essentially comprise the characteristic C-terminal 'alpha-crystallin domain' of the sHSPs, having a very short N-terminal region, and lacking a C-terminal tail. Recombinant Hsp12.2 and...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Kokke BP,Leroux MR,Candido EP,Boelens WC,de Jong WW

    更新日期:1998-08-21 00:00:00

  • Glyoxalase I of the malarial parasite Plasmodium falciparum: evidence for subunit fusion.

    abstract::Recombinant Plasmodium falciparum glyoxalase I (PfGlx I) was characterized as monomeric Zn(2+)-containing enzyme of 44 kDa. The K(M) value of the methylglyoxal-glutathione adduct is 77+/-15 microM, the k(cat) value being 4000 min(-1) at 25 degrees C and pH 7.0. PfGlx I consists of two halves, each of which is homologo...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Iozef R,Rahlfs S,Chang T,Schirmer H,Becker K

    更新日期:2003-11-20 00:00:00

  • The SAAS granin exhibits structural and functional homology to 7B2 and contains a highly potent hexapeptide inhibitor of PC1.

    abstract::Prohormone convertases (PCs) 1 and 2 are thought to mediate the proteolytic cleavage of many peptide precursors. Endogenous inhibitors of both PC1 and PC2 have now been identified; the 7B2 protein is a nanomolar inhibitor of PC2, while the novel protein proSAAS was recently reported to be a micromolar inhibitor of PC1...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Cameron A,Fortenberry Y,Lindberg I

    更新日期:2000-05-12 00:00:00

  • 'Neuron-specific' protein gene product 9.5 (PGP 9.5) is also expressed in glioma cell lines and its expression depends on cellular growth state.

    abstract::Protein gene product 9.5 (PGP 9.5), which in the normal nervous system is restricted to certain neurons, has been detected in two glioma cell lines, rat C6 and human GL15, by immunoblotting and immunocytochemistry. Its expression in these cells depends on the cellular growth state, being maximal between the first and ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Giambanco I,Bianchi R,Ceccarelli P,Pula G,Sorci G,Antonioli S,Bocchini V,Donato R

    更新日期:1991-09-23 00:00:00

  • Differential expression of intestinal membrane transporters in cholera patients.

    abstract::Vibrio cholerae causes the cholera disease through secretion of cholera toxin (CT), resulting in severe diarrhoea by modulation of membrane transporters in the intestinal epithelium. Genes encoding membrane-spanning transporters identified as being differentially expressed during cholera disease in a microarray screen...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Flach CF,Qadri F,Bhuiyan TR,Alam NH,Jennische E,Holmgren J,Lönnroth I

    更新日期:2007-07-10 00:00:00

  • Calcium mobilization by angiotensin II and neurotransmitter receptors expressed in Xenopus laevis oocytes.

    abstract::Specific receptors for angiotensin II (AII) were expressed in albino Xenopus laevis oocytes co-injected with poly(A)+ mRNA isolated from rat adrenal cortex and the calcium-specific photoprotein, aequorin. In such oocytes, AII elicited rapid, dose-dependent rises in cytosolic free calcium with light emission responses ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Sandberg K,Markwick AJ,Trinh DP,Catt KJ

    更新日期:1988-12-05 00:00:00

  • Role of genome guardian proteins in transcriptional elongation.

    abstract::Maintaining genomic integrity is vital for cell survival and homeostasis. Mutations in critical genes in germ-line and somatic cells are often implicated with the onset or progression of diseases. DNA repair enzymes thus take important roles as guardians of the genome in the cell. Besides the known function to repair ...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审


    authors: Bunch H

    更新日期:2016-04-01 00:00:00

  • The induction of early response genes in rat smooth muscle cells by PDGF-AA is not sufficient to stimulate DNA-synthesis.

    abstract::The effect of the three platelet-derived growth factor (PDGF) isoforms AA, AB and BB on the induction of the early growth response genes c-fos, egr-1 and c-myc mRNA in vascular smooth muscle cells from rat was compared with their respective mitogenic potency. The three PDGF isoforms strongly stimulated the induction t...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Sachinidis A,Schulte K,Ko Y,Meyer zu Brickwedde MK,Hoppe V,Hoppe J,Vetter H

    更新日期:1993-03-22 00:00:00

  • Auxin-induced reactive oxygen species production requires the activation of phosphatidylinositol 3-kinase.

    abstract::We recently reported that production of reactive oxygen species (ROS) is essential for auxin-induced gravitropic signaling. Here, we investigated the role of phosphatidylinositol 3-kinase and its product, PtdIns(3)P, in auxin-mediated ROS production and the root gravitropic response. Pretreatment with LY294002, an inh...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Joo JH,Yoo HJ,Hwang I,Lee JS,Nam KH,Bae YS

    更新日期:2005-02-14 00:00:00

  • The role of long-chain acyl-CoA in the damage of oxidative phosphorylation in heart mitochondria.

    abstract::The aim of this investigation was to study the effect of intramitochondrial acyl-CoA on the respiration of rabbit heart mitochondria over the whole range of stationary respiratory rates between States 4 and 3. The creatine phosphokinase system was used for stabilization of extramitochondrial adenine nucleotide concent...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Borutaite V,Mildaziene V,Ivanoviene L,Kholodenko B,Toleikis A,Praskevicius A

    更新日期:1989-01-30 00:00:00

  • The interaction of amino-deuteromethylated melittin with phospholipid membranes studied by deuterium NMR.

    abstract::Melittin, deuteromethylated on each of the four amino groups (Gly-1 N alpha and Lys-7, 21, and 23 N epsilon), was prepared by reductive methylation using deuteroformaldehyde and NaBD3CN. Deuterium NMR spectra were obtained for the modified peptide (D-melittin) bound to phospholipid bilayers and erythrocyte ghosts. D-M...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Dempsey CE,Cryer GD,Watts A

    更新日期:1987-06-22 00:00:00

  • Sarcoplasmic reticulum release channels from frog skeletal muscle display two types of calcium dependence.

    abstract::Calcium channels derived from sarcoplasmic reticulum of frog skeletal muscle were fused with planar lipid bilayers. Fractional open times displayed two types of calcium dependence: (i) blockable channels showed a bell-shaped calcium dependence with an activation constant of 4.5 microM, a Hill coefficient for activatio...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Bull R,Marengo JJ

    更新日期:1993-10-04 00:00:00

  • Tumor necrosis factor is markedly synergistic with interleukin 1 and interferon-gamma in stimulating the production of nerve growth factor in fibroblasts.

    abstract::A possible interaction between tumor necrosis factor-alpha (TNF) and other cytokines/growth factors in stimulating the production of nerve growth factor (NGF) in Swiss 3T3 cells was studied. TNF's stimulatory activity on fibroblast NGF production was synergized by interleukin-1 alpha (IL-1 alpha), IL-1 beta and interf...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Hattori A,Iwasaki S,Murase K,Tsujimoto M,Sato M,Hayashi K,Kohno M

    更新日期:1994-03-07 00:00:00

  • The antioxidative function of eicosapentaenoic acid in a marine bacterium, Shewanella marinintestina IK-1.

    abstract::When the eicosapentaenoic acid (EPA)-deficient mutant strain IK-1Delta8 of the marine EPA-producing Shewanella marinintestina IK-1 was treated with various concentrations of hydrogen peroxide (H(2)O(2)), its colony-forming ability decreased more than that of the wild type. Protein carbonylation, induced by treating ce...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Nishida T,Morita N,Yano Y,Orikasa Y,Okuyama H

    更新日期:2007-09-04 00:00:00

  • Nuclear phosphoproteins HMGA and their relationship with chromatin structure and cancer.

    abstract::The structural characteristics of the three nuclear phosphoproteins of the high mobility group A family are outlined and related to their participation in chromatin structure alteration in many biological processes such as gene expression, neoplastic transformation, differentiation, and apoptosis. The elevated express...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审


    authors: Sgarra R,Rustighi A,Tessari MA,Di Bernardo J,Altamura S,Fusco A,Manfioletti G,Giancotti V

    更新日期:2004-09-10 00:00:00

  • The mitogenic signaling pathway for fibroblast growth factor-2 involves the tyrosine phosphorylation of cyclin D2 in MCF-7 human breast cancer cells.

    abstract::Fibroblast growth factor-2 (FGF-2) is mitogenic for the human breast cancer cell line MCF-7; here we investigate some of the signaling pathways subserving this activity. FGF-2 stimulation of MCF-7 cells resulted in a global increase of intracellular tyrosine phosphorylation of proteins, particularly FGF receptor subst...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Vercoutter-Edouart A,Lemoine J,Smart CE,Nurcombe V,Boilly B,Peyrat J,Hondermarck H

    更新日期:2000-08-04 00:00:00

  • In vivo glycosylation suppresses the aggregation of amyloidogenic hen egg white lysozymes expressed in yeast.

    abstract::The mutant hen egg white lysozymes Ile55Thr and Asp66His, corresponding to human amyloidogenic mutant lysozymes Ile56Thr and Asp67His, respectively, were secreted in Saccharomyces cerevisiae. The amyloidogenic mutants (I55T and D66H) of hen egg white lysozymes were remarkably less soluble than that of the wild-type pr...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Song Y,Azakami H,Hamasu M,Kato A

    更新日期:2001-02-23 00:00:00

  • Locked 5Zs-biliverdin blocks the Meta-RA to Meta-RC transition in the functional cycle of bacteriophytochrome Agp1.

    abstract::The bacteriophytochrome Agp1 was reconstituted with a locked 5Zs-biliverdin in which the C(4)=C(5) and C(5)-C(6) bonds of the methine bridge between rings A and B are fixed in the Z and syn configuration/conformation, respectively. In Agp1-5Zs the photoconversion proceeds via the Lumi-R intermediate to Meta-R(A), but ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Seibeck S,Borucki B,Otto H,Inomata K,Khawn H,Kinoshita H,Michael N,Lamparter T,Heyn MP

    更新日期:2007-11-27 00:00:00

  • Requirement of intact human ceruloplasmin for the glutathione-linked peroxidase activity.

    abstract::Structural integrity may be needed for the glutathione-linked peroxidase activity of human ceruloplasmin. Intact human ceruloplasmin has a potent peroxidase property to decompose H2O2 in the presence of reduced glutathione. However, the fragment of approximately 116000 Da produced by proteolytic degradation had less t...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Kim IG,Park SY

    更新日期:1998-10-23 00:00:00

  • Attenuated cytotoxicity but enhanced betafibril of a mutant amyloid beta-peptide with a methionine to cysteine substitution.

    abstract::Amyloid-beta peptide (Abeta), the major constituent of senile plaques in the Alzheimer's disease (AD) brain, is the main source of oxidative stress leading to neurodegeneration. The methionine residue in this peptide is reported to be responsible for neurotoxicity. Structurally similar substitution with methionine 35 ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Dai XL,Sun YX,Jiang ZF

    更新日期:2007-04-03 00:00:00

  • Arginine kinase overexpression improves Trypanosoma cruzi survival capability.

    abstract::Arginine kinase catalyzes the reversible transphosphorylation between adenosine diphosphate (ADP) and phosphoarginine, which is involved in temporal and spatial adenosine triphosphate (ATP) buffering. Here we demonstrate that the homologous overexpression of the Trypanosoma cruzi arginine kinase improves the ability o...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Pereira CA,Alonso GD,Ivaldi S,Silber AM,Alves MJ,Torres HN,Flawiá MM

    更新日期:2003-11-06 00:00:00

  • A comparison of demethoxyviridin and wortmannin as inhibitors of phosphatidylinositol 3-kinase.

    abstract::The mammalian Ptdlns 3-kinase is shown to be inhibited by low nanomolar concentrations of demethoxyviridin, an antifungal agent structurally related to wortmannin. The inhibitory potency of both compounds could be observed in purified Ptdlns 3-kinase whether or not the regulatory subunit (p85 alpha) was present, sugge...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Woscholski R,Kodaki T,McKinnon M,Waterfield MD,Parker PJ

    更新日期:1994-04-04 00:00:00

  • Tissue inhibitor of metalloproteinases from human bone marrow stromal cell line KM 102 has erythroid-potentiating activity, suggesting its possibly bifunctional role in the hematopoietic microenvironment.

    abstract::In this study, we demonstrated that tissue inhibitor of metalloproteinases (TIMP) produced by human bone marrow stromal cell line KM-102 had erythroid-potentiating activity (EPA) which stimulates the proliferation of erythroid progenitor cells. We, then, propose a scheme for the bifunctional role of TIMP/EPA in hemato...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Hayakawa T,Yamashita K,Kishi J,Harigaya K

    更新日期:1990-07-30 00:00:00