Heme incorporation into the cytochrome bo3 occurs at a late stage of assembly.

abstract：:The trend of the electric field and the value of the electric field flux, sensed by the superoxide substrate in the proximity of the active site, were found to be constant in three highly homologous Cu,Zn superoxide dismutases from ox, pig and sheep, which display large differences in net protein charge and distributi...

journal_title：FEBS letters

authors： Desideri A,Falconi M,Parisi V,Rotilio G

更新日期：1989-06-19 00:00:00

abstract：:Autolysis of the Ca(2+)-dependent cysteine protease m-calpain involves cleavage of the large (80 kDa) and small (30 kDa) subunits of the enzyme, and an increase in Ca2+ sensitivity. The appearance of increased Ca2+ sensitivity was found to correlate with the cleavage of the large subunit after residue 9. ...

journal_title：FEBS letters

authors： Brown N,Crawford C

更新日期：1993-05-03 00:00:00

abstract：:Heat shock proteins (Hsps) are induced in response to various kinds of environmental and physiological stresses. However, it is unclear whether Hsps play roles in protecting cells in the digestive organs against xenobiotic chemicals. Here, we found that feeding induces expression of a set of Hsps specifically in the m...

journal_title：FEBS letters

authors： Katsuki K,Fujimoto M,Zhang XY,Izu H,Takaki E,Tanizawa Y,Inouye S,Nakai A

更新日期：2004-07-30 00:00:00

abstract：:Glycoprotein synthesis is initiated in the endoplasmic reticulum (ER) lumen upon transfer of a glycan (Glc3Man9GlcNAc2) from a lipid derivative to Asn residues (N-glycosylation). N-Glycan-dependent quality control of glycoprotein folding in the ER prevents exit to Golgi of folding intermediates, irreparably misfolded ...

journal_title：FEBS letters

authors： Caramelo JJ,Parodi AJ

更新日期：2015-11-14 00:00:00

abstract：:We have studied whether the cooperative insecticidal effect of certain scorpion toxin pairs, namely either a combination of excitatory and depressant, or alpha and depressant scorpion toxins, would improve the efficacy of Autographa californica nucleopolyhedrovirus (AcMNPV) over a virus expressing only a single toxin,...

journal_title：FEBS letters

authors： Regev A,Rivkin H,Inceoglu B,Gershburg E,Hammock BD,Gurevitz M,Chejanovsky N

更新日期：2003-02-27 00:00:00

abstract：:α-Synuclein (αSyn) is a key player in the pathogenesis of Parkinson's disease and other synucleinopathies. Here, we report the existence of a novel soluble α-helical conformer of αSyn, obtained through transient interaction with lipid interfaces, and propose dynamic oligomerization as the mechanism underlying its stab...

journal_title：FEBS letters

authors： Rovere M,Sanderson JB,Fonseca-Ornelas L,Patel DS,Bartels T

更新日期：2018-05-01 00:00:00

abstract：:The alpha3/beta4 subtype of neuronal nicotinic acetylcholine receptor (nAChR) was stably expressed in human embryonic kidney (HEK) 293 cells that co-expressed a voltage-gated Ca2+ channel. alpha3/beta4-nAChR-expressing clones were identified using the fura-2 Ca2+ imaging technique, and were further characterised by si...

journal_title：FEBS letters

authors： Stetzer E,Ebbinghaus U,Storch A,Poteur L,Schrattenholz A,Kramer G,Methfessel C,Maelicke A

更新日期：1996-11-11 00:00:00

abstract：:A simple method for isolation and fluorescence labelling of Leydig cells (L-cells) from rat testes was developed. Lateral diffusion coefficients of both lipid and protein membrane fluorescent probes were measured by the method of fluorescence recovery after photobleaching (FRAP). Age-dependent changes in diffusibility...

journal_title：FEBS letters

authors： Zlatanov IV,Foley M,Birmingham J,Garland PB

更新日期：1987-09-28 00:00:00

abstract：:L-Type calcium channel was expressed in Xenopus laevis oocytes injected with RNAs coding for different cardiac Ca2+ channel subunits, or with total heart RNA. The effects of activation of protein kinase C (PKC) by the phorbol ester PMA (4 beta-phorbol 12-myristate 13-acetate) were studied. Currents through channels co...

journal_title：FEBS letters

authors： Singer-Lahat D,Gershon E,Lotan I,Hullin R,Biel M,Flockerzi V,Hofmann F,Dascal N

更新日期：1992-07-20 00:00:00

abstract：:The phorbol esters are among the most potent tumor promoters. On addition of 12-O-tetradecanoylphorbol 13-acetate (TPA) to isolated human platelets prelabelled with [32P]orthophosphate we found a rapid increase in 32P incorporation into phosphatidylinositol 4-phosphate and phosphatidylinositol 4,5-bisphosphate. In vie...

journal_title：FEBS letters

authors： de Chaffoy de Courcelles D,Roevens P,van Belle H

更新日期：1984-08-06 00:00:00

abstract：:Amylin appears to interfere with the action of insulin in muscle and possibly in liver. We have attempted to detect a direct antagonism between amylin and insulin in cultured rat hepatocytes. The stimulation of glucokinase gene expression was used as a marker of insulin action. Amylin proved ineffective in suppressing...

journal_title：FEBS letters

authors： Nouspikel T,Gjinovci A,Li S,Iynedjian PB

更新日期：1992-04-13 00:00:00

abstract：:Microdissected, beta-cell-rich pancreatic islets from ob/ob mice were used in studies of 86Rb+ transport. D-Glucose (20 mM) induced a biphasic reduction in 86Rb+ efflux. The reduction stabilized within 10 min at 34% of the efflux rate at zero glucose. The initial 86Rb+ uptake (5 min) was dose-dependently reduced by ou...

journal_title：FEBS letters

authors： Lindström P,Norlund L,Sehlin J

更新日期：1986-05-05 00:00:00

abstract：:The effect of the three platelet-derived growth factor (PDGF) isoforms AA, AB and BB on the induction of the early growth response genes c-fos, egr-1 and c-myc mRNA in vascular smooth muscle cells from rat was compared with their respective mitogenic potency. The three PDGF isoforms strongly stimulated the induction t...

journal_title：FEBS letters

authors： Sachinidis A,Schulte K,Ko Y,Meyer zu Brickwedde MK,Hoppe V,Hoppe J,Vetter H

更新日期：1993-03-22 00:00:00

abstract：:The chemotherapeutic activity of three synthetic antibacterial peptides was investigated. KLKLLLLLKLK-NH2 and its D-enantiomer showed significant chemotherapeutic activity in MRSA-infected mice, whereas KLKLLLKLK-NH2, which showed the highest antibacterial activity among them in vitro, was found to have almost no abil...

journal_title：FEBS letters

authors： Nakajima Y,Alvarez-Bravo J,Cho J,Homma K,Kanegasaki S,Natori S

更新日期：1997-09-22 00:00:00

abstract：:Photoaffinity labelling with [alpha-32P]GTP allowed to detect a 54 kDa GTP-binding protein in rat pancreatic plasma membranes and in pancreatic AR 4-2J cell membranes. Like the 42 and 48 kDa Gs alpha subunits and the 41 kDa Gi alpha subunit, this protein was absent from zymogen granule membranes. Contrastingly, a new ...

journal_title：FEBS letters

authors： Lambert M,Bui ND,Christophe J

更新日期：1990-10-01 00:00:00

abstract：:Capsaicin, a major ingredient of hot pepper, is considered to exhibit anti-inflammatory properties. Our previous study demonstrated that capsaicin inhibited the production of pro-inflammatory mediators through NF-kappaB inactivation in LPS-stimulated macrophages. In order to further clarify the mechanism underlying th...

journal_title：FEBS letters

authors： Park JY,Kawada T,Han IS,Kim BS,Goto T,Takahashi N,Fushiki T,Kurata T,Yu R

更新日期：2004-08-13 00:00:00

abstract：:Vacuolar membrane H(+)-translocating pyrophosphatase (H(+)-PPase) was purified from pumpkin seedlings. Its enzymatic properties including molecular size of constituting polypeptide (75 kDa) were very similar to those of mung bean H(+)-PPase [(1989) J. Biol. Chem. 264, 20068-20073]. The native, functional molecular siz...

journal_title：FEBS letters

authors： Sato MH,Maeshima M,Ohsumi Y,Yoshida M

更新日期：1991-09-23 00:00:00

abstract：:Low density lipoproteins are highly sensitive to oxidation by copper salts, and such peroxidation is accompanied by macrophage scavenger receptor recognition. This study shows that fresh human atherosclerotic material (aneurysms and endarterectomies) can contain detectable amounts of redox active iron and copper that ...

journal_title：FEBS letters

authors： Swain J,Gutteridge JM

更新日期：1995-07-24 00:00:00

abstract：:Sequential reverse transcription and polymerase chain reaction (RT-PCR) of the mRNA were used to investigate the expression of dopamine receptors in human peripheral blood lymphocytes. The RT-PCR products contained three types of sequences, each corresponding to those of the D5 dopamine receptor gene and the two relat...

journal_title：FEBS letters

authors： Takahashi N,Nagai Y,Ueno S,Saeki Y,Yanagihara T

更新日期：1992-12-07 00:00:00

abstract：:Treatment of the chemically transformed fibroblasts BP-A31 and other cell lines with low concentrations of cycloheximide (CHM) for 72 h followed by the removal of the protein synthesis inhibitor leads to the proliferation of alpha-difluoromethylornithine (DFMO)-resistant phenotypes. These drug-resistant cells contain ...

journal_title：FEBS letters

authors： Medrano EE,Burrone OR,Ferrer MM,Cafferata EG,Algranati ID

更新日期：1986-09-29 00:00:00

abstract：:Treatment of rat thymus cell suspensions with dexamethasone resulted in inhibition of engaged RNA polymerase A, without significant change in free pool activity. Studies with the re-initiation inhibitor, rifamycin AF/0-13, and measurements of numbers of RNA polymerase A molecules and of elongation rates showed that th...

journal_title：FEBS letters

authors： Dembinski TC

更新日期：1984-07-23 00:00:00

abstract：:Mitochondrial thioredoxin (mtTrx) can be oxidized in response to inducers of oxidative stress; yet the functional consequences of the oxidation have not been determined. This study evaluated the redox status of mtTrx and its association to oxidant-induced apoptosis. Results showed that mtTrx was oxidized after exposur...

journal_title：FEBS letters

authors： Chen Y,Cai J,Jones DP

更新日期：2006-12-11 00:00:00

abstract：:Transfer of naked plasmid with insulin precursor DNA into skeletal muscle of streptozotocin (STZ)-induced diabetic mice through electroporation and detection of gene expression is described. Four different human insulin precursor DNA fragments were inserted into pcDNA3.1(-), downstream of a CMV promoter. Three of them...

journal_title：FEBS letters

authors： Yin D,Tang JG

更新日期：2001-04-20 00:00:00

abstract：:Mouse embryonic stem (ES) cells homozygous for disruption of the MSK1 gene had no detectable MSK1 activity. However, their activators (extracellular signal related kinase (ERK)1/ERK2) were stimulated normally in mitogen- and stress-activated protein kinase (MSK)1-/- and wild type cells in response to tetradecanoylphor...

journal_title：FEBS letters

authors： Arthur JS,Cohen P

更新日期：2000-09-29 00:00:00

abstract：:Targeting of preproteins to mitochondria and their translocation across the outer membrane are mediated by the mitochondrial receptor complex. This protein complex contains the import receptors MOM19 and MOM72 and the general insertion pore GIP. All seven components of the receptor complex are synthesized in the cytos...

journal_title：FEBS letters

authors： Keil P,Pfanner N

更新日期：1993-04-26 00:00:00

abstract：:Steroids are of universal occurrence, present variously as cell wall constituents and bioregulators. A number of bacteria, fungi, and photosynthetic vascular plants synthesize steroids that are hormonally active in the animal world. The cellular effect of such steroids in microbes and plants appears by and large to be...

journal_title：FEBS letters

authors： Agarwal MK

更新日期：1993-05-17 00:00:00

abstract：:Phorbol-esters have been shown to modulate the beta-adrenergic-stimulated adenylyl cyclase in a number of cell lines. Here, using site directed mutagenesis, we investigate the role of the beta 2-adrenergic receptor phosphorylation by protein kinase C in this regulatory process. Mutation of the serine-261, -262, -344 a...

journal_title：FEBS letters

authors： Bouvier M,Guilbault N,Bonin H

更新日期：1991-02-25 00:00:00

abstract：:The Bacillus thuringiensis Cry toxin causes swelling and necrosis in insect cells, but the route(s) and significance of the water influx involved in its cytotoxicity are unclear. Here, we assessed the role of aquaporins (AQPs), known as water channels, in Cry toxin intoxication. An AQP inhibitor did not interfere with...

journal_title：FEBS letters

authors： Endo H,Azuma M,Adegawa S,Kikuta S,Sato R

更新日期：2017-01-01 00:00:00

abstract：:An efficient sensing of danger and a rapid activation of the immune system are crucial for the survival of plants. Conserved pathogen/microbe-associated molecular patterns (PAMPs/MAMPs) and endogenous molecular patterns, which are present only when the tissue is infected or damaged (damage-associated molecular pattern...

journal_title：FEBS letters

authors： De Lorenzo G,Brutus A,Savatin DV,Sicilia F,Cervone F

更新日期：2011-06-06 00:00:00

abstract：:Guanylate cyclase activity was measured in disrupted rod outer segments of the toad retina. The experiments showed that cGMP is synthesized from GTP at a rate of 3 +/- 1 nmol/min per mg protein. In darkness this value is largely independent of the Ca2+ concentration, while it is enhanced by flashes of light of increas...

journal_title：FEBS letters

authors： Pepe IM,Panfoli I,Cugnoli C

更新日期：1986-07-14 00:00:00