Feeding induces expression of heat shock proteins that reduce oxidative stress.

abstract：:Using a BAL31 exonuclease assay to determine the sites of 4,5',8-trimethylpsoralen photocrosslinking in DNA we have shown that 5'-TA sites which are accessible to psoralen DNA interstrand photocrosslinking in naked DNA become inaccessible when protein, in casu, lambda-repressor E. coli or RNA polymerase are bound at t...

journal_title：FEBS letters

authors： Zhen WP,Jeppesen C,Nielsen PE

更新日期：1988-02-29 00:00:00

abstract：:A novel rapid polymerase chain reaction (PCR)-based technique for N-terminal attachment of green fluorescent protein (GFP) to a yeast protein is described. Genomic integration of a PCR-generated loxPkanMX4loxP-yEGFP fusion cassette immediately upstream of the open reading frame (ORF) allows for selection of G418 resis...

journal_title：FEBS letters

authors： Prein B,Natter K,Kohlwein SD

更新日期：2000-11-17 00:00:00

abstract：:Micromolar concentrations of arachidonic acid cause in Ca2+ loaded heart mitochondria matrix swelling and Ca2+ release. These effects appear to be unrelated to the classical membrane permeability transition (MPT), as they are CsA insensitive, membrane potential independent and can also be activated by Sr2+. Atractylos...

journal_title：FEBS letters

authors： Di Paola M,Zaccagnino P,Oliveros-Celis C,Lorusso M

更新日期：2006-02-06 00:00:00

abstract：:Human endothelial cells exposed to H2O2 showed reduced CREP DNA binding activity, enhanced HSF activation, and no induction of NFkappaB binding activity. Interestingly, H2O2 was able to induce NFkappaB subunit p65 translocation in the nucleus. In contrast, cells exposed to TNF alpha showed enhanced CREP binding activi...

journal_title：FEBS letters

authors： Jornot L,Petersen H,Junod AF

更新日期：1997-10-27 00:00:00

abstract：:The initial processes of platelet activation by platelet-activating factor (PAF) were observed by stopped-flow light scattering and fluorometry. The binding of PAF to rabbit platelets did not change the membrane fluidity, though it caused the removal of calcium from internal stores and induced concomitantly platelet s...

journal_title：FEBS letters

authors： Ohga Y,Nakanishi M

更新日期：1986-10-20 00:00:00

abstract：:Cellular retinoic acid binding proteins (CRABPs) bind all-trans-retinoic acid (atRA) tightly. This study aimed to determine whether atRA is channeled directly to cytochrome P450 (CYP) CYP26B1 by CRABPs, and whether CRABPs interact directly with CYP26B1. atRA bound to CRABPs (holo-CRABP) was efficiently metabolized by ...

journal_title：FEBS letters

authors： Nelson CH,Peng CC,Lutz JD,Yeung CK,Zelter A,Isoherranen N

更新日期：2016-08-01 00:00:00

abstract：:Protein kinase C (PKC) may be involved in growth regulation. In the present study the relationship between body weight, and thereby age, and the activity of PKC in muscle as well as in rapidly growing overloaded muscle were investigated. PKC activity in music was linearly inversely correlated to rat weight in both sol...

journal_title：FEBS letters

authors： Richter EA,Nielsen NB

更新日期：1991-09-02 00:00:00

abstract：:Immunoblotting analysis of purified human urokinase plasminogen activator (u-PA), gives a positive signal when reacted with anti-phosphotyrosine monoclonal antibodies (MoAb anti-P-Tyr); competition with o-phospho-DL-tyrosine (P-Tyr) but not o-phospho-DL-threonine or serine (P-Treo, P-Ser) completely suppresses this si...

journal_title：FEBS letters

authors： Barlati S,Paracini F,Bellotti D,De Petro G

更新日期：1991-04-09 00:00:00

abstract：:In Alzheimer's disease and related dementias, human tau protein aggregates into paired helical filaments and neurofibrillary tangles. However, such tau aggregates have not yet been demonstrated in transgenic mouse models of the disease. One of the possible explanations would be that mouse tau has different properties ...

journal_title：FEBS letters

authors： Kampers T,Pangalos M,Geerts H,Wiech H,Mandelkow E

更新日期：1999-05-14 00:00:00

abstract：:The activity of breast milk BSDL was assayed with or without phospholipids as extra-intestinal effector candidates. Phosphatidic acid, lysophosphatidic acid and platelet activating factor but not phosphatidylcholine and lysophosphatidylcholine stimulated BSDL activity at least as efficiently as taurocholate. The appar...

journal_title：FEBS letters

authors： Fontbonne H,Puigserver A,Bouza B,Lombardo D,Ajandouz el H

更新日期：2013-09-17 00:00:00

abstract：:Epigallocatechin-3-gallate (EGCG) is a major component of green tea polyphenols which displays potential properties of anticancer and neuroprotection. Here we show that protection of motor neuron by EGCG is associated with regulating glutamate level in organotypic culture of rat spinal cord. In this model, EGCG blocke...

journal_title：FEBS letters

authors： Yu J,Jia Y,Guo Y,Chang G,Duan W,Sun M,Li B,Li C

更新日期：2010-07-02 00:00:00

abstract：:Because A2 adenosine receptor activation stimulates adenylate cyclase and cyclic AMP induces 5'-nucleotidase expression in rat mesangial cells, we examined the effect of adenosine and its analogs on 5'-nucleotidase activity in these cells. A2 adenosine receptors were characterized using [3H]5'-N-ethylcarboxamidoadenos...

journal_title：FEBS letters

authors： Stefanovic V,Vlahovic P,Savic V,Ardaillou N,Ardaillou R

更新日期：1993-09-27 00:00:00

abstract：:Deproteinized globin poly(A) + mRNAs reassociate readily in vitro with soluble RNA-binding proteins of the cytosol; reconstituted messenger ribonucleoprotein complexes are obtained which are very similar to native globin polyribosomal-mRNP as far as bouyant density in Cs2SO4 and the composition of proteins which can b...

journal_title：FEBS letters

authors： Gaedigk R,Oehler S,Köhler K,Setyono B

更新日期：1985-01-07 00:00:00

abstract：:Apomyoglobin was found to break down phosphatidylcholine vesicles to form a micellar complex at pH 4. Size reduction during this process was observed by measuring light scattering at 400 nm and by passing the complex through a gel-filtration column. Micellar complex formation was confirmed by electron microscopy. ...

journal_title：FEBS letters

authors： Lee JW,Kim H

更新日期：1988-12-05 00:00:00

abstract：:The mitochondrial myopathy, encephalopathy, lactic acidosis and stroke-like episodes syndrome (MELAS) is a rare congenital disorder of mitochondrial DNA. Five single nucleotide substitutions within the human mitochondrial tRNALeu(UUR) gene have been reported to be associated with MELAS. Here, we provide in vitro evide...

journal_title：FEBS letters

authors： Hao R,Yao YN,Zheng YG,Xu MG,Wang ED

更新日期：2004-12-03 00:00:00

abstract：:Basic fibroblast growth factor (FGF-2) is an important modulator of cell growth and differentiation under both physiological and pathological conditions. Until recently, most investigations into the FGF-2 signalling pathway were concerned with its interaction with specific membrane receptors. Nevertheless, while a 18 ...

journal_title：FEBS letters

authors： Delrieu I

更新日期：2000-02-18 00:00:00

abstract：:The ability of three fatty acids and their respective ethyl esters, to promote generation of reactive oxygen species (ROS), was compared in a preparation of rat brain synaptosomes. Arachidonic but not palmitic or linoleic acids promoted ROS generation. Ethyl esterification of each fatty acid significantly enhanced ROS...

journal_title：FEBS letters

authors： Bondy SC,Marwah S

更新日期：1995-11-13 00:00:00

abstract：:Disulfide bond formation is a critical step in the folding of many secretory proteins. In bacteria, disulfide bonds are introduced by the periplasmic dithiol oxidase DsbA, which transfers its catalytic disulfide bond to folding polypeptides. Reduced DsbA is reoxidized by ubiquinone Q8, catalyzed by inner membrane quin...

journal_title：FEBS letters

authors： Malojcić G,Owen RL,Grimshaw JP,Glockshuber R

更新日期：2008-10-15 00:00:00

abstract：:Organisms using the thiosulfate-oxidizing Sox enzyme system fall into two groups: group 1 forms sulfur globules as intermediates (Allochromatium vinosum), group 2 does not (Paracoccus pantotrophus). While several components of their Sox systems are quite similar, i.e. the proteins SoxXA, SoxYZ and SoxB, they differ by...

journal_title：FEBS letters

authors： Welte C,Hafner S,Krätzer C,Quentmeier A,Friedrich CG,Dahl C

更新日期：2009-04-17 00:00:00

abstract：:In order to elucidate the molecular mechanisms responsible for the specific lipid-protein interactions, we have undertaken structural and idiotypic analyses of a monoclonal antibody, PS4A7, which binds specifically to phosphatidylserine (PS). Here we showed that one of the anti-idiotypic monoclonal antibodies raised a...

journal_title：FEBS letters

authors： Reza F,Igarashi K,Tokita S,Asai K,Aoki J,Asaoka Y,Umeda M,Inoue K

更新日期：1994-02-21 00:00:00

abstract：:The absorption frequencies of the C = O and C = C (neutral state) and of the C...O (semiquinone state) stretching vibrations of QB have been assigned by FTIR spectroscopy, using native and site-specifically 1-, 2-, 3- and 4-13C-labelled ubiquinone-10 (UQ10) reconstituted at the QB binding site of Rhodobacter sphaeroid...

journal_title：FEBS letters

authors： Brudler R,de Groot HJ,van Liemt WB,Gast P,Hoff AJ,Lugtenburg J,Gerwert K

更新日期：1995-08-14 00:00:00

abstract：:The synthetic cRNA encoding for the major T lymphocyte K+ channel (Kv1.3) was injected into Xenopus fertilized eggs. Somites from embryos of stage 20-22 (about 40 h post-fertilization at 19 degrees C) were dissociated and myotomal muscle cells were cultured in vitro for 2 days. The whole cell configuration of the tigh...

journal_title：FEBS letters

authors： Honoré E,Guillemare E,Lesage F,Barhanin J,Lazdunski M

更新日期：1994-07-18 00:00:00

abstract：:Endothelial monocyte-activating polypeptide II (EMAP II) is a proinflammatory cytokine and a chemoattractant for leukocytes. The mature cytokine is formed in apoptotic cells by cleavage of the precursor proEMAP II. Here we show that caspase-7 is capable of cleaving proEMAP II in vitro. A proEMAP II mutant, in which th...

journal_title：FEBS letters

authors： Behrensdorf HA,van de Craen M,Knies UE,Vandenabeele P,Clauss M

更新日期：2000-01-21 00:00:00

abstract：:Vacuolar tandem-pore channels could not be analysed in Xenopus oocytes so far, due to misguided translocation. Owing to the conservation of their pore regions, we were able to prepare functional pore-chimeras between the plasma membrane localised TPK4 and vacuolar TPKs. Thereby, we found evidence that TPK2, TPK3 and T...

journal_title：FEBS letters

authors： Marcel D,Müller T,Hedrich R,Geiger D

更新日期：2010-06-03 00:00:00

abstract：:We have set up a model system for familial amyotrophic lateral sclerosis (FALS) by transfecting human neuroblastoma cell line SH-SY5Y with plasmids directing constitutive expression of either wild-type human Cu,Zn superoxide dismutase (Cu,ZnSOD) or a mutant of this enzyme (G93A) associated with FALS. We have tested mi...

journal_title：FEBS letters

authors： Carrì MT,Ferri A,Battistoni A,Famhy L,Gabbianelli R,Poccia F,Rotilio G

更新日期：1997-09-08 00:00:00

abstract：:The amino acid sequence of EcoRV DNA methyltransferase which methylates the amino group of the 5'-adenine residue of the target sequence GATATC has been found to be closely related to that of three other adenine methyltransferases, DpnII, dam and damT4, the target sequence of which is GATC. Despite large differences o...

journal_title：FEBS letters

authors： Lauster R,Kriebardis A,Guschlbauer W

更新日期：1987-08-10 00:00:00

abstract：:By using monoiodinated radioligands of both intact neuropeptide Y (NPY) and of a long C-terminal fragment, NPY13-36, two subtypes of binding sites, which differ in affinity and specificity, have been characterized. The Y1 type of binding site, characterized on a human neuroblastoma cell line, MC-IXC, and a rat pheochr...

journal_title：FEBS letters

authors： Sheikh SP,Håkanson R,Schwartz TW

更新日期：1989-03-13 00:00:00

abstract：:Fourier-transform infrared (FT-IR) spectral imaging was used for analyzing biochemical changes in tumor cells. Metabolic parameters of human lung A549/8 adenocarcinoma and U87 glioma cells were compared under stress conditions in culture along with tumor progression after cell implantation onto the chick embryo chorio...

journal_title：FEBS letters

authors： Petibois C,Drogat B,Bikfalvi A,Déléris G,Moenner M

更新日期：2007-11-27 00:00:00

abstract：:The enzymatic degradation of the closely related insoluble polysaccharides; cellulose (β(1-4)-linked glucose) by cellulases and chitin (β(1-4)-linked N-acetylglucosamine) by chitinases, is of large biological and economical importance. Processive enzymes with different inherent directionalities, i.e. attacking the pol...

journal_title：FEBS letters

authors： Hamre AG,Schaupp D,Eijsink VG,Sørlie M

更新日期：2015-07-08 00:00:00

abstract：:The reduction of methyl-coenzyme M (CH3SCoM) to methane in methanogenic bacteria is dependent on component B (N-7-mercaptoheptanoyl-O-phospho-L-threonine, HSHTP). We report here that S-methyl-component B (N-7-(methylthio)heptanoyl-O-phospho-L-threonine, CH3SHTP) can substitute for neither CH3SCoM nor HSHTP in the meth...

journal_title：FEBS letters

authors： Ellermann J,Kobelt A,Pfaltz A,Thauer RK

更新日期：1987-08-17 00:00:00