Abstract:
:Using a BAL31 exonuclease assay to determine the sites of 4,5',8-trimethylpsoralen photocrosslinking in DNA we have shown that 5'-TA sites which are accessible to psoralen DNA interstrand photocrosslinking in naked DNA become inaccessible when protein, in casu, lambda-repressor E. coli or RNA polymerase are bound at their recognition DNA sequences (OR1 operator or deo1 promoter, respectively). These results show that psoralens can be used as photofootprinting reagents to study specific protein-DNA interactions.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Zhen WP,Jeppesen C,Nielsen PEdoi
10.1016/0014-5793(88)80800-7subject
Has Abstractpub_date
1988-02-29 00:00:00pages
73-6issue
1eissn
0014-5793issn
1873-3468pii
0014-5793(88)80800-7journal_volume
229pub_type
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