Abstract:
:Human endothelial cells exposed to H2O2 showed reduced CREP DNA binding activity, enhanced HSF activation, and no induction of NFkappaB binding activity. Interestingly, H2O2 was able to induce NFkappaB subunit p65 translocation in the nucleus. In contrast, cells exposed to TNF alpha showed enhanced CREP binding activity, activation of NFkappaB and no induction of HSE-HSF complex. Addition of H2O2, diamide and iodoacetic acid to the binding reaction mixture markedly reduced the DNA binding ability of the three transcription factors. Thus free sulfhydryls were important in DNA binding activity of CREP, NFkappaB and HSF, and the lack of induction of NFkappaB by H2O2 in intact cells was likely caused by oxidation on a thiol, and not by a deficiency in the activation pathway.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Jornot L,Petersen H,Junod AFdoi
10.1016/s0014-5793(97)01244-1subject
Has Abstractpub_date
1997-10-27 00:00:00pages
381-6issue
3eissn
0014-5793issn
1873-3468pii
S0014-5793(97)01244-1journal_volume
416pub_type
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