Abstract:
:Early results showed the emanation of chromatin fibers from mitotic chromosomes and nuclei swollen with water. In contrast, under metaphase ionic conditions, it was found that chromatin from mitotic chromosomes is planar and forms multilayered plates. Here, we show that in buffers containing interphase cation concentrations, the chromatin emanated from disrupted nuclei also has a planar morphology. Furthermore, the chromatin fragments produced by micrococcal nuclease digestion of nuclei form the typical beads-on-a-string fibers in the absence of cations, but they self-assemble into plate-like structures in buffers containing magnesium. The plates from interphase nuclei do not form the thick multilayered structures observed in metaphase chromosomes, suggesting that they are more exposed to the medium to facilitate DNA replication and gene expression.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Chicano A,Daban JRdoi
10.1002/1873-3468.13370subject
Has Abstractpub_date
2019-04-01 00:00:00pages
810-819issue
8eissn
0014-5793issn
1873-3468journal_volume
593pub_type
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