Fibroblast growth factor-10 upregulates Na,K-ATPase via the MAPK pathway.


:We studied the effects of fibroblast growth factor (FGF-10) on alveolar epithelial cell (AEC) Na,K-ATPase regulation. Within 30 min FGF-10 increased Na,K-ATPase activity and alpha1 protein abundance by 2.5-fold at the AEC plasma membrane. Pretreatment of AEC with the mitogen-activated protein kinase (MAPK) inhibitor U0126, a Grb2-SOS inhibitor (SH3-b-p peptide), or a Ras inhibitor (farnesyl transferase inhibitor (FTI 277)), as well as N17-AEC that express a Ras dominant negative protein each prevented FGF-10-mediated Na,K-ATPase recruitment to the AEC plasma membrane. Accordingly, we provide first evidence that FGF-10 upregulates (short-term) the Na,K-ATPase activity in AEC via the Grb2-SOS/Ras/MAPK pathway.




FEBS letters


Upadhyay D,Lecuona E,Comellas A,Kamp DW,Sznajder JI





Has Abstract


2003-06-19 00:00:00














  • Inositol trisphosphate-induced hyperpolarization in rat dorsal root ganglion neurons.

    abstract::Inositol 1,4,5-trisphosphate (1,4,5-InsP3) was perfused into rat dorsal root ganglion (DRG) neurons by whole-cell patch-clamp electrodes, while measuring the membrane potential. This operation evoked a transient (2-3 min) membrane hyperpolarization of about -15 mV (from -42 mV) followed by a depolarization. The membra...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Wang Z,Ypey DL,Van Duijn B

    更新日期:1992-06-15 00:00:00

  • Reduction of mitochondrial tRNALeu(UUR) aminoacylation by some MELAS-associated mutations.

    abstract::The mitochondrial myopathy, encephalopathy, lactic acidosis and stroke-like episodes syndrome (MELAS) is a rare congenital disorder of mitochondrial DNA. Five single nucleotide substitutions within the human mitochondrial tRNALeu(UUR) gene have been reported to be associated with MELAS. Here, we provide in vitro evide...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Hao R,Yao YN,Zheng YG,Xu MG,Wang ED

    更新日期:2004-12-03 00:00:00

  • SP-40,40, a protein involved in the control of the complement pathway, possesses a unique array of disulphide bridges.

    abstract::SP-40,40 is a two-chain serum protein which acts in vitro as a potent inhibitor of the assembly of the membrane attack complex of human complement. It contains 10 cysteine residues, the numbers and locations of which are conserved in several mammalian species. Evidence is presented that all the cysteine residues are i...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Kirszbaum L,Bozas SE,Walker ID

    更新日期:1992-02-03 00:00:00

  • Dictyostelium discoideum requires an Alix/AIP1 homolog, DdAlix, for morphogenesis in alkaline environments.

    abstract::Alix and its homologs are involved in various phenomena such as endosomal protein-sorting and adaptation to stress conditions. In this study, we found that development of Dictyostelium discoideum Alix (DdAlix) deletion mutant (alx-) cells was impaired in alkaline pH environments. The fruiting body formation efficiency...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Ohkouchi S,Saito H,Aruga F,Maeda T,Shibata H,Maki M

    更新日期:2005-03-14 00:00:00

  • Cloning of a human liver UDP-glucose pyrophosphorylase cDNA by complementation of the bacterial galU mutation.

    abstract::A human liver cDNA clone which encodes the UDP-glucose pyrophosphorylase was isolated by complementation of a bacterial galU mutant. The deduced amino acid sequence of the human enzyme comprised 508 amino acids with a calculated molecular mass of 56,950. The human enzyme significantly resembles those of potato tuber a...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Peng HL,Chang HY

    更新日期:1993-08-23 00:00:00

  • Torus-shaped particles formed due to intermolecular condensation of circular DNA upon interaction with synthetic tripeptide.

    abstract::The morphology of complexes between relaxed circular plasmid pBR322 DNA and tripeptide L-Val-L-Val-L-Val-NH-NH-Dns (TVP) at different peptide/DNA ratios was studied by electron microscopy. The results show that interaction of TVP with circular DNA leads to the formation of perfect torus-shaped particles. The torus par...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Vengerov YY,Semenov TE,Streltsov SA,Makarov VL,Khorlin AA,Gursky GV

    更新日期:1985-01-21 00:00:00

  • A recessive mutant cell line with a constitutive IkappaB kinase activity.

    abstract::To search for negative regulatory components of the NF-kappaB activation pathways, we mutagenized Rat-1 fibroblasts and established a stable mutant cell line with a constitutive NF-kappaB activity. This mutant cell line, designated as TK26, showed permanently elevated I kappa B kinase (IKK) activity and a genetically ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Chinanonwait N,Miura H,Yamamoto N,Yamaoka S

    更新日期:2002-11-20 00:00:00

  • The cis/trans interconversion of the calcium regulating hormone calcitonin is catalyzed by cyclophilin.

    abstract::The cytosolic peptidyl-prolyl cis/trans isomerase cyclophilin from pig kidney can accelerate catalytically the cis/trans isomerization of prolyl peptide bonds. One- and two-dimensional 1H NMR spectroscopy was used to prove that the polypeptide hormone calcitonin is a substrate for cyclophilin. Isomerization of only on...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Kern D,Drakenberg T,Wikström M,Forsén S,Bang H,Fischer G

    更新日期:1993-06-01 00:00:00

  • Involvement of phospholipase D in oxidative stress-induced necrosis of vascular smooth muscle cells.

    abstract::Phospholipase D (PLD) has been associated with necrosis. However, it is not clear whether PLD plays a causative role in this cellular process. We investigated the role of PLD in oxidative stress-induced necrosis of vascular smooth muscle cells (VSMCs). Pervanadate (hydrogen peroxide plus orthovanadate) but not hydroge...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Shin EY,Min DS,Shin JC,Shin KS,Hyun MS,Ha KS,Kim HS,Ahn HY,Kim EG

    更新日期:2001-11-16 00:00:00

  • Dual effects of G-protein activation on Ca-dependent exocytosis in bovine lactotrophs.

    abstract::The whole-cell patch-clamp technique was used to measure cell membrane capacitance (Cm) to monitor exocytosis in single-cultured bovine prolactin-secreting cells (lactotrophs) of the anterior pituitary. The cells were dialyzed with solutions containing different concentrations of ionised Ca and non-hydrolyzable GTP an...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Sikdar SK,Zorec R,Brown D,Mason WT

    更新日期:1989-08-14 00:00:00

  • Functional conservation of mouse Notch receptor family members.

    abstract::All the known members of the mouse Notch receptor family were examined for their biochemical function by interaction with a DNA binding protein RBP-Jkappa. mNotch2, mNotch3 and int3 (= mNotch4) were shown to interact with RBP-Jkappa by the GST-fusion pull down assay and dominant negative competition with Epstein Barr ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Kato H,Sakai T,Tamura K,Minoguchi S,Shirayoshi Y,Hamada Y,Tsujimoto Y,Honjo T

    更新日期:1996-10-21 00:00:00

  • A novel ovary-specific and ovulation-associated variant of epoxide hydrolase 2.

    abstract::Ovulation is a complex process initiated by the surge of the pituitary luteinizing hormone (LH) that provokes the expression of specific genes. We report herein the isolation and characterization of an ovulation-associated, ovary-specific novel isoform of epoxide hydrolase 2 (Ephx2), Ephx2C. This variant is exclusivel...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Shkolnik K,Ben-Dor S,Galiani D,Hourvitz A,Dekel N

    更新日期:2007-10-16 00:00:00

  • Downregulation of collagen synthesis in fibroblasts within three-dimensional collagen lattices involves transcriptional and posttranscriptional mechanisms.

    abstract::Culturing human fibroblasts in a three-dimensional collagen matrix leads to a reduction of collagen I by more than 90%, both on the level of mRNA steady-state as well as protein. In order to differentiate changes in de novo transcription and posttranscriptional control, nuclear run on assays and pulse/chase experiment...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Eckes B,Mauch C,Hüppe G,Krieg T

    更新日期:1993-03-01 00:00:00

  • Re-entering the pluripotent state from blood lineage: promises and pitfalls of blood reprogramming.

    abstract::Blood reprogramming, in which induced pluripotent stem cells (iPSCs) are derived from haematopoietic lineages, has rapidly advanced over the past decade. Since the first report using human blood, haematopoietic cell types from various sources, such as the peripheral bone marrow and cord blood, have been successfully r...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审


    authors: Chen Y,Yi Y,Xu J,Chan WK,Loh YH

    更新日期:2019-12-01 00:00:00

  • Human pancreatic phospholipase A2 stimulates the growth of human pancreatic cancer cell line.

    abstract::Phospholipase A2 (PLA2) from human pancreas, designated hPLA2-I, functions as a digestive enzyme. Interestingly, the present study demonstrated that the mature form of hPLA2-I stimulated the growth of a human pancreatic cancer cell line MIAPaCa-2, whereas the pro-form was ineffective. PLA2s from Laticauda semifasciata...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Hanada K,Kinoshita E,Itoh M,Hirata M,Kajiyama G,Sugiyama M

    更新日期:1995-10-02 00:00:00

  • High cholesterol diet induces tau hyperphosphorylation in apolipoprotein E deficient mice.

    abstract::We analysed the effects of high cholesterol (HC) intake and reduced apolipoprotein E (apoE) activity on tau phosphorylation and on the activities of the major tau kinases and phosphatases in brains from wild-type and apoE-knockout (apoEKO) mice. We show that HC diet potently induced intraneuronal accumulation of hyper...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Rahman A,Akterin S,Flores-Morales A,Crisby M,Kivipelto M,Schultzberg M,Cedazo-Mínguez A

    更新日期:2005-11-21 00:00:00

  • Regulation of ubiquitous 6-phosphofructo-2-kinase by the ubiquitin-proteasome proteolytic pathway during myogenic C2C12 cell differentiation.

    abstract::6-Phosphofructo-2-kinase catalyzes the synthesis and degradation of fructose 2,6-bisphosphate, activator of phosphofructokinase-1 and inhibitor of fructose 1,6-bisphosphatase. These properties confer to this bifunctional enzyme a key role in the control of glycolysis and gluconeogenesis. Several mammalian isozymes gen...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Riera L,Obach M,Navarro-Sabaté A,Duran J,Perales JC,Viñals F,Rosa JL,Ventura F,Bartrons R

    更新日期:2003-08-28 00:00:00

  • The catalytic power of magnesium chelatase: a benchmark for the AAA(+) ATPases.

    abstract::In the first committed reaction of chlorophyll biosynthesis, magnesium chelatase couples ATP hydrolysis to the thermodynamically unfavorable Mg(2+) insertion into protoporphyrin IX (ΔG°' of circa 25-33 kJ·mol(-1) ). We explored the thermodynamic constraints on magnesium chelatase and demonstrate the effect of nucleoti...

    journal_title:FEBS letters

    pub_type: 信件


    authors: Adams NB,Brindley AA,Hunter CN,Reid JD

    更新日期:2016-06-01 00:00:00

  • Functional analysis of an indole-diterpene gene cluster for lolitrem B biosynthesis in the grass endosymbiont Epichloë festucae.

    abstract::Epichloë festucae Fl1 in association with Lolium perenne synthesizes a diverse range of indole-diterpene bioprotective metabolites, including lolitrem B, a potent tremorgen. The ltm genes responsible for the synthesis of these metabolites are organized in three clusters at a single sub-telomeric locus in the genome of...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Saikia S,Takemoto D,Tapper BA,Lane GA,Fraser K,Scott B

    更新日期:2012-07-30 00:00:00

  • Functional and molecular characterization of a peptide transporter in the rat PC12 neuroendocrine cell line.

    abstract::We have studied functional properties of peptide transport in the pheochromocytoma neuroendocrine cell line from rat. The neutral peptide D-Phe-L-Ala (resistant to hydrolysis) is a good substrate for uptake into these cells. Transport is substantially inhibited by diethylpyrocarbonate pretreatment and is stimulated by...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Hussain I,Zanic-Grubisic T,Kudo Y,Boyd CA

    更新日期:2001-11-23 00:00:00

  • Evidence for cell surface association between CXCR4 and ganglioside GM3 after gp120 binding in SupT1 lymphoblastoid cells.

    abstract::CXCR4 (fusin) is a chemokine receptor which is involved as a coreceptor in gp120 binding to the cell surface. In this study we provide evidence that binding of gp120 triggers CXCR4 recruitment to glycosphingolipid-enriched microdomains. Scanning confocal microscopy showed a nearly complete localization of CXCR4 within...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Sorice M,Garofalo T,Misasi R,Longo A,Mattei V,Sale P,Dolo V,Gradini R,Pavan A

    更新日期:2001-09-28 00:00:00

  • Human REG family genes are tandemly ordered in a 95-kilobase region of chromosome 2p12.

    abstract::Reg, first isolated from a rat regenerating islet cDNA library, is expressed in regenerating islet beta-cells. Recently, it has been revealed that Reg and Reg-related genes constitute a multigene family, the Reg family. In human, the four REG family genes, i.e., REG 1 alpha, REG 1 beta, REG-related sequence (RS) and H...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Miyashita H,Nakagawara K,Mori M,Narushima Y,Noguchi N,Moriizumi S,Takasawa S,Yonekura H,Takeuchi T,Okamoto H

    更新日期:1995-12-27 00:00:00

  • Effect of antipsychotic drugs on the molecular action of cholera toxin in rabbit intestinal epithelial cells.

    abstract::Antipsychotic drugs of known antidiarrhoeal and anticalmodulin activity inhibited the cholera-toxin-catalysed ADP-ribosylation of proteins of Mr 37,000, 40,000 and 45,000 (thought to be regulatory components of the adenylate cyclase complex) that was previously shown to occur in plasma membranes from rabbit intestinal...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Longbottom D,van Heyningen S

    更新日期:1990-10-15 00:00:00

  • Transforming growth factor-beta1 induces activation of Ras, Raf-1, MEK and MAPK in rat hepatic stellate cells.

    abstract::The transdifferentiation of hepatic stellate cells into myofibroblast-like cells and the proliferation of the transdifferentiated cells are controlled by TGF-beta1. Little is known about the intracellular signal transducers of TGF-beta1. In this paper we show that in cultured hepatic stellate cells TGF-beta1 induces a...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Reimann T,Hempel U,Krautwald S,Axmann A,Scheibe R,Seidel D,Wenzel KW

    更新日期:1997-02-10 00:00:00

  • Denervation-induced spatiotemporal upregulation of ephrin-A2 in the mouse hippocampus after transections of the perforant path.

    abstract::Transections of the entorhinal afferent fibers to hippocampus, perforant path (PP), result in the denervation in specific hippocampal subregions, which is followed by a series of plastic events including axon sprouting and reactive synaptogenesis. Many growth-associated molecules are thought to participate in these ev...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Wang Y,Ni ZM,Zhou CF

    更新日期:2005-02-14 00:00:00

  • Molecular characterization of a novel human PDZ domain protein with homology to INAD from Drosophila melanogaster.

    abstract::PDZ domains are thought to act as protein-binding modules mediating the clustering of membrane and membrane-associated proteins. The INAD protein has been shown to interact via a PDZ domain with the calcium channel TRP which contributes to capacitative calcium entry into Drosophila photoreceptor cells. We have cloned ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Philipp S,Flockerzi V

    更新日期:1997-08-18 00:00:00

  • Metabolism of acetaldehyde in human and baboon renal cortex. Ethanol synthesis by isolated baboon kidney-cortex tubules.

    abstract::Acetaldehyde (1-20 mM) was metabolized at high rates and in a dose-dependent manner in isolated human and baboon kidney-cortex tubules. Acetaldehyde removal was accompanied by a large accumulation of acetate in both human and baboon tubules. By contrast, a large synthesis of ethanol was observed only in baboon tubules...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Michoudet C,Baverel G

    更新日期:1987-05-25 00:00:00

  • cDNA cloning and inducible expression of human multidrug resistance associated protein 3 (MRP3).

    abstract::Previously, we cloned rat MRP3 as a candidate for an inducible transporter for the biliary excretion of organic anions [Hirohashi et al. (1998) Mol. Pharmacol. 53, 1068-10751. In the present study, we cloned human MRP3 (1527 amino acids) from Caco-2 cells. Human MRP3 is predominantly expressed in liver, small intestin...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Kiuchi Y,Suzuki H,Hirohashi T,Tyson CA,Sugiyama Y

    更新日期:1998-08-14 00:00:00

  • Specific inhibition of Na-Ca exchange function by antisense oligodeoxynucleotides.

    abstract::The Na-Ca exchanger is essential for the Ca2+ homeostasis in many cell types. This transporter has been difficult to investigate because no specific inhibitor is available. We have synthesized an antisense oligodeoxynucleotide directed against the rat cardiac Na-Ca exchanger mRNA. To estimate the activity of the Na-Ca...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Lipp P,Schwaller B,Niggli E

    更新日期:1995-05-08 00:00:00

  • An endoplasmic reticulum/plasma membrane junction: STIM1/Orai1/TRPCs.

    abstract::Ca(2+) entering cells through store-operated channels (SOCs) affects most cell functions, and excess SOC is associated with pathologies. The molecular makeup of SOCs and their mechanisms of gating were clarified with the discovery of the Orais and STIM1. Another form of SOCs are the TRPCs. STIM1 gates both Orai and TR...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审


    authors: Lee KP,Yuan JP,Hong JH,So I,Worley PF,Muallem S

    更新日期:2010-05-17 00:00:00