Abstract:
:Plasmid pGC20 containing the (dGC)9 insert in SmaI recognition site has been used to study the inhibition of cleavage by different restriction endonuclease due to Z-DNA formation in (dCG)10 sequence of the negatively supercoiled plasmid. Data obtained indicate the different sensitivity of restriction endonucleases to DNA conformational perturbations resulted from the Z-DNA formation. Therefore, the inhibition of DNA cleavage by a particular restriction endonuclease cannot serve as a criterion for the estimation of the length of B-Z junctions in circular supercoiled DNAs.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Lesnik EA,Bes'chetnikova ZhA,Maslova RN,Varshavsky JaMdoi
10.1016/0014-5793(91)80211-ksubject
Has Abstractpub_date
1991-03-11 00:00:00pages
91-3issue
1eissn
0014-5793issn
1873-3468journal_volume
280pub_type
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