Abstract:
:Hepatocyte tight junctional permeability has been shown to be regulated by hormones that exert their effects via phospholipase C activation. However, the precise transduction pathway involved in this effect is not known. The present study has employed the selective inhibitor of microsomal Ca2+ sequestration, 2,5-di(tert-butyl)-1,4-benzohydroquinone (tBuBHQ), to examine the effect of the mobilization of the endoplasmic reticular Ca2+ pool on tight junctional permeability in the perfused rat liver. Infusion of tBuBHQ followed by a bolus infusion of horseradish peroxidase (HRP) resulted in a significant increase in the first peak of biliary HRP, a measure of junctional permeability, whereas transcellular (vesicular) transport of HRP was not affected. Therefore, we conclude that the effect of hormones on tight junctional permeability is mediated, at least in part, by the mobilization of intracellular Ca2+.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Llopis J,Kass GE,Duddy SK,Farell GC,Gahm A,Orrenius Sdoi
10.1016/0014-5793(91)80209-lsubject
Has Abstractpub_date
1991-03-11 00:00:00pages
84-6issue
1eissn
0014-5793issn
1873-3468pii
0014-5793(91)80209-Ljournal_volume
280pub_type
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