Possible role of immune surveillance at the initial phase of metastasis produced by B16BL6 melanoma cells.

abstract：:Uptake of cholesterol by isolated rat hepatocytes in a serum-free medium was remarkably enhanced by dispersion with several kinds of phosphatidylcholine. Of the various phosphatidylcholines tested, dilinoylphosphatidylcholine had the strongest accelerating effect, while dipalmitoylphosphatidylcholine was the weakest. ...

journal_title：FEBS letters

authors： Nagamura Y,Sasaki K,Ito M,Saito K,Ogitsu N,Ishiguro I

更新日期：1986-07-14 00:00:00

abstract：:Chimeric proteins composed of ricin toxin A chain (RTA) and staphylococcal protein A (PA) have been produced in E. coli. Constructs consisting of N-terminal RTA and C-terminal PA (RTA-PA) or N-terminal PA and C-terminal (PA-RTA) were capable of binding to immunoglobulin G (via PA) and of specifically depurinating 28 S...

journal_title：FEBS letters

authors： O'Hare M,Brown AN,Hussain K,Gebhardt A,Watson G,Roberts LM,Vitetta ES,Thorpe PE,Lord JM

更新日期：1990-10-29 00:00:00

abstract：:Extracellular ATP itself elicited the generation of superoxide (O2-) in guinea pig peritoneal macrophages associated with an increase in cytosolic calcium ([Ca2+]i). The ATP-induced O2- generation was completely inhibited by pretreatment with pertussis toxin (PT) accompanied by the suppression of [Ca2+]i mobilization....

journal_title：FEBS letters

authors： Nakanishi M,Takihara H,Minoru Y,Yagawa K

更新日期：1991-04-22 00:00:00

abstract：:The Arabidopsis thalina genome database was searched for homologues of the Candida cloacae fao1 gene which encodes a membrane bound, flavin-containing, hydrogen peroxide generating, long chain alcohol oxidase. This gene has not been isolated from plants or animals. Four putative candidates were found in the database b...

journal_title：FEBS letters

authors： Cheng Q,Liu HT,Bombelli P,Smith A,Slabas AR

更新日期：2004-09-10 00:00:00

abstract：:The bacterial twin-arginine translocation (Tat) pathway is distinct from the Sec system by its remarkable capacity to export folded enzymes. To address the question whether the two systems are capable of translocating homologous enzymes catalyzing the same reaction, we cloned the tap gene encoding Thermus thermophilus...

journal_title：FEBS letters

authors： Angelini S,Moreno R,Gouffi K,Santini C,Yamagishi A,Berenguer J,Wu L

更新日期：2001-10-05 00:00:00

abstract：:Methyl-beta-cyclodextrin (MbetaCD) was used to explore a role for cholesterol-enriched plasma membrane microdomains in coupling lysophosphatidic acid (LPA) stimulation to phosphoinositide 3-kinase (PI3K) activation. Cholesterol depletion strongly inhibited the production of phosphatidylinositol 3,4-bisphosphate and ph...

journal_title：FEBS letters

authors： Peres C,Yart A,Perret B,Salles JP,Raynal P

更新日期：2003-01-16 00:00:00

abstract：:Eukaryotic porins are a group of membrane proteins whose best known role is to form an aqueous pore channel in the mitochondrial outer membrane. As opposed to the bacterial porins (a large family of protein whose 3D structure has been determined by X-ray diffraction), the structure of eukaryotic porins (also termed VD...

journal_title：FEBS letters

authors： Casadio R,Jacoboni I,Messina A,De Pinto V

更新日期：2002-06-05 00:00:00

abstract：:Human parainfluenza virus type 3 (hPIV3) recognizes both α2,3- and α2,6-linked sialic acids, whereas human parainfluenza virus type 1 (hPIV1) recognizes only α2,3-linked sialic acids. To identify amino acid residues that confer α2,6-linked sialic acid recognition of hPIV3, amino acid residues in or neighboring the sia...

journal_title：FEBS letters

authors： Fukushima K,Takahashi T,Ueyama H,Takaguchi M,Ito S,Oishi K,Minami A,Ishitsubo E,Tokiwa H,Takimoto T,Suzuki T

更新日期：2015-05-08 00:00:00

abstract：:Using oligonucleotide primers derived from the vesicular monoamine transporters sequences, a cDNA predicted to encode the bovine chromaffin granule amine transporter has been cloned (b-VMAT2). Surprisingly, its structure is more similar to the rat brain transporter (VMAT2), than to the rat adrenal counterpart (VMAT1)....

journal_title：FEBS letters

authors： Howell M,Shirvan A,Stern-Bach Y,Steiner-Mordoch S,Strasser JE,Dean GE,Schuldiner S

更新日期：1994-01-24 00:00:00

abstract：:A stable complex of the chaperonins, cpn60 and cpn10 (Escherichia coli GroEL and GroES homologues), from the extremely thermophilic bacterium Thermus thermophilus has been isolated and crystallized. The crystals have dimensions up to 30 x 200 x 200 microns. Ultra-thin sections of the crystals estimated by electron mic...

journal_title：FEBS letters

authors： Lissin NM,Sedelnikova SE,Ryazantsev SN

更新日期：1992-10-12 00:00:00

abstract：:Type II restriction endonucleases (REases) exist in multiple oligomeric forms. The tetrameric REases have two DNA binding interfaces and must synapse two recognition sites to achieve cleavage. It was hypothesised that binding of two recognition sites by tetrameric enzymes contributes to their fidelity. Here, we experi...

journal_title：FEBS letters

authors： Zaremba M,Sasnauskas G,Siksnys V

更新日期：2012-09-21 00:00:00

abstract：:Studies were undertaken to further elucidate the mechanism(s) by which bradykinin-dependent phosphoinositide metabolism takes place in neuroblastoma X glioma hybrid NG108-15 cells [(1984) J. Biol. Chem. 259, 10201-10207] using [3H]inositol-labelled cells. Bradykinin produced net increases in the level of [3H]inositol ...

journal_title：FEBS letters

authors： Yano K,Higashida H,Hattori H,Nozawa Y

更新日期：1985-02-25 00:00:00

abstract：:An approach is presented for the determination of the branch location of 1 or 2 extra N-acetyllactosamine units in sialo N-linked carbohydrate chains from glycoproteins. Tetraantennary oligosaccharides containing extra N-acetyllactosamine units were digested with endo-beta-galactosidase, followed by treatment with N-a...

journal_title：FEBS letters

authors： Hokke CH,Kamerling JP,van Dedem GW,Vliegenthart JF

更新日期：1991-07-29 00:00:00

abstract：:Wild-type trypanosomal triosephosphate isomerase (wtTIM) is a very tight dimer. The interface residue His-47 of wtTIM has been mutated into an asparagine. Ultracentrifugation data show that this variant (H47N) only dimerises at protein concentrations above 3 mg/ml. H47N has been characterised at a protein concentratio...

journal_title：FEBS letters

authors： Borchert TV,Zeelen JP,Schliebs W,Callens M,Minke W,Jaenicke R,Wierenga RK

更新日期：1995-07-03 00:00:00

abstract：:The Escherichia coli twin-arginine translocation (Tat) system serves to export fully folded protein substrates across the bacterial cytoplasmic membrane. Respiratory [NiFe] hydrogenases are synthesised as precursors with twin-arginine signal peptides and transported as large, cofactor-containing, multi-subunit complex...

journal_title：FEBS letters

authors： Dubini A,Sargent F

更新日期：2003-08-14 00:00:00

abstract：:A human vesicular monoamine transporter cDNA has been identified by screening a human brainstem library using sequences from the rat brain synaptic vesicle monoamine transporter (SVMT) [(1992) Cell 70, 539-551; (1992) Proc. Natl. Acad. Sci. USA 89, 10993-10997]. The hSVMT shares 92% amino acid identity with the rat se...

journal_title：FEBS letters

authors： Surratt CK,Persico AM,Yang XD,Edgar SR,Bird GS,Hawkins AL,Griffin CA,Li X,Jabs EW,Uhl GR

更新日期：1993-03-08 00:00:00

abstract：:The myo-inositol 1,4,5-trisphosphate (IP3)-induced Ca2+ mobilization in single saponin-permeabilized and fura-2-loaded parietal cells was analysed by a fluorescence digital image processor. When the cells were incubated with ATP, free cytoplasmic Ca2+ concentration [( Ca2+]i) increased in some restricted cytoplasmic r...

journal_title：FEBS letters

authors： Tsunoda Y,Yodozawa S,Tashiro Y

更新日期：1988-04-11 00:00:00

abstract：:NG-Methyl-L-arginine (L-NMA) and NG-nitro-L-arginine (L-NNA) inhibited NO-induced cGMP accumulation in porcine aortic endothelial cells with half-maximally effective concentrations of 15 and 3.4 microM, respectively. The effects of both compounds were reversible, but the L-NNA-induced inhibition was only reversed by w...

journal_title：FEBS letters

authors： Mayer B,Schmid M,Klatt P,Schmidt K

更新日期：1993-10-25 00:00:00

abstract：:CpG dinucleotides are efficiently methylated in vertebrate genomes except in the CpG islands having a high C+G content. Methylated CpGs are the single most mutated dinucleotide. Sequences surrounding disease causing CpG mutation sites were analyzed from locus-specific mutation databases. Both tetra- and heptanucleotid...

journal_title：FEBS letters

authors： Ollila J,Lappalainen I,Vihinen M

更新日期：1996-11-04 00:00:00

abstract：:Snake venoms are rich sources of serine proteinase inhibitors that are members of the Kunitz/BPTI (bovine pancreatic trypsin inhibitor) family. However, only a few of their gene sequences have been determined from snakes. We therefore cloned the cDNAs for the trypsin and chymotrypsin inhibitors from a Vipera ammodytes...

journal_title：FEBS letters

authors： Zupunski V,Kordis D,Gubensek F

更新日期：2003-07-17 00:00:00

abstract：:D-Aspartate increases human chorionic gonadotropin-induced testosterone production in purified rat Leydig cells. L-Aspartate, D-,L-glutamate or D-,L-asparagine could not substitute for D-aspartate and this effect was independent of glutamate receptor activation. Testosterone production was enhanced only in cells cultu...

journal_title：FEBS letters

authors： Nagata Y,Homma H,Lee JA,Imai K

更新日期：1999-02-12 00:00:00

abstract：:HIV gp120 is specifically cleaved at a single site in the V3 loop between Arg315 and Ala316 by thrombin. Previous observations by others have indicated that binding to CD4 enhances the rate of V3 loop cleavage, and that this cleavage is a prerequisite for HIV infection. Other observations also suggest that the cleavag...

journal_title：FEBS letters

authors： Johnson ME,Lin Z,Padmanabhan K,Tulinsky A,Kahn M

更新日期：1994-01-03 00:00:00

abstract：:Genetic factors seem to play a significant role in susceptibility to systemic lupus erythematosus (SLE). We previously described the amino acid polymorphism (Val14Met) within the IFN-gamma receptor 1 (IFN-gammaRI), and that the frequency of the Metl4 allele in SLE patients was significantly higher than that of the hea...

journal_title：FEBS letters

authors： Nakashima H,Inoue H,Akahoshi M,Tanaka Y,Yamaoka K,Ogami E,Nagano S,Arinobu Y,Niiro H,Otsuka T,Niho Y

更新日期：1999-06-18 00:00:00

abstract：:We recently reported that production of reactive oxygen species (ROS) is essential for auxin-induced gravitropic signaling. Here, we investigated the role of phosphatidylinositol 3-kinase and its product, PtdIns(3)P, in auxin-mediated ROS production and the root gravitropic response. Pretreatment with LY294002, an inh...

journal_title：FEBS letters

authors： Joo JH,Yoo HJ,Hwang I,Lee JS,Nam KH,Bae YS

更新日期：2005-02-14 00:00:00

abstract：:F-box proteins play pivotal roles in multiple cellular processes; however, little is known about their functions in glioma progression. In this study, we found that expression of the F-box and leucine-rich repeat protein 18 (FBXL18) is significantly upregulated in glioma tissues. Depletion of FBXL18 in glioma cells su...

journal_title：FEBS letters

authors： Zhang J,Yang Z,Ou J,Xia X,Zhi F,Cui J

更新日期：2017-01-01 00:00:00

abstract：:Accumulation of hyperphosphorylated Tau is associated with a number of neurodegenerative diseases collectively known as tauopathies. Differences in clinical and cognitive profiles among them suggest differential sensitivity of neuronal populations to Tau levels, phosphorylation and mutations. We used tissue specific e...

journal_title：FEBS letters

authors： Grammenoudi S,Kosmidis S,Skoulakis EM

更新日期：2006-08-21 00:00:00

abstract：:'Peripheral' binding sites for benzodiazepines are under neural or homonal control in the pineal gland, olfactory bulb, and kidney. These observations prompted a search for an endogenous substance which could modulate these sites under physiological conditions. Acidified methanol extracts from several tissues (e.g. st...

journal_title：FEBS letters

authors： Mantione CR,Weissman BA,Goldman ME,Paul SM,Skolnick P

更新日期：1984-10-15 00:00:00

abstract：:We report the cDNA-deduced primary structure of the chicken counterpart of the murine dilute gene product, a member of the myosin I family. Comparison of the chicken and mouse sequences reveals a distinct pattern of domains of high and low sequence conservation. An internal deletion of 25 amino acids probably reflects...

journal_title：FEBS letters

authors： Sanders G,Lichte B,Meyer HE,Kilimann MW

更新日期：1992-10-26 00:00:00

abstract：:The two subunits of gamma-glutamyl transpeptidase (EC 2.3.2.2) are derived from a single-chain glycosylated precursor. A small fraction of the propeptide survives proteolytic processing in the rat kidney and has been purified by an immunoaffinity technique. The propeptide contains determinants for both the subunits an...

journal_title：FEBS letters

authors： Tate SS

更新日期：1986-01-01 00:00:00

abstract：:The cytochrome bc1 complex is a key component of the mitochondrial respiratory chains of many eukaryotic microorganisms that are pathogenic for plants or humans, such as fungi responsible for crop diseases and Plasmodium falciparum, which causes human malaria. Cytochrome bc1 is an enzyme that contains two (ubi)quinone...

journal_title：FEBS letters

authors： Fisher N,Meunier B,Biagini GA

更新日期：2020-09-01 00:00:00