Abstract:
:We recently reported that production of reactive oxygen species (ROS) is essential for auxin-induced gravitropic signaling. Here, we investigated the role of phosphatidylinositol 3-kinase and its product, PtdIns(3)P, in auxin-mediated ROS production and the root gravitropic response. Pretreatment with LY294002, an inhibitor of PtdIns 3-kinase activity, blocked auxin-mediated ROS generation, and reduced the sensitivity of root tissue to gravistimulation. The amount of PtdIns(3)P increased in response to auxin, and this effect was abolished by pretreatment with LY294002. In addition, sequestration of PtdIns(3)P by transient expression of the endosome binding domain in protoplasts abrogated IAA-induced ROS accumulation. These results indicate that activation of PtdIns 3-kinase and its product PtdIns(3)P are required for auxin-induced production of ROS and root gravitropism.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Joo JH,Yoo HJ,Hwang I,Lee JS,Nam KH,Bae YSdoi
10.1016/j.febslet.2005.01.018keywords:
subject
Has Abstractpub_date
2005-02-14 00:00:00pages
1243-8issue
5eissn
0014-5793issn
1873-3468pii
S0014-5793(05)00100-6journal_volume
579pub_type
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