Ionic regulation of cyclic AMP levels in Paramecium tetraurelia in vivo.

abstract：:Streptomyces lividans 1326-9, a bldA+ strain, and its bldA39 mutant derivative J1725 were transformed with a cosmid containing the pur cluster, which determines the puromycin biosynthetic pathway from Streptomyces alboniger. bldA+ transformants produced puromycin in typical amounts, whereas bldA39 transformants did so...

journal_title：FEBS letters

authors： Tercero JA,Espinosa JC,Jiménez A

更新日期：1998-01-16 00:00:00

abstract：:G2/M checkpoints ensure the proper timing of cell mitosis. We previously reported that p38 mitogen-activated protein kinase (MAPK) activation is essential for stress-induced G2 arrest in the U-2OS osteosarcoma cell line, but the molecular mechanism was obscure. Here, using the T7 phage display system, we find p38 dire...

journal_title：FEBS letters

authors： Wu W,Zhou H,He F,Xiao Z,Jiang Y,Zhao M

更新日期：2018-12-01 00:00:00

abstract：:Previously, several studies have demonstrated that autophosphorylation of the C-terminal tyrosine residues (Tyr1316 and Tyr1322) affects the signaling properties of the insulin receptor in vivo. To assess the biochemical consequences of the C-terminal phosphorylation in vitro, we have constructed, purified and charact...

journal_title：FEBS letters

authors： Tennagels N,Bergschneider E,Al-Hasani H,Klein HW

更新日期：2000-08-11 00:00:00

abstract：:Retinal dehydrogenase (RALDH) catalyzes the oxidation of retinal to all-trans and 9-cis retinoic acid, which function as ligands controlling RAR and RXR nuclear receptor-signaling pathways. We have recently shown the expression of RALDH transcript in the stomach and small intestine by reverse transcription polymerase ...

journal_title：FEBS letters

authors： Bhat PV

更新日期：1998-04-17 00:00:00

abstract：:Mycobacterium tuberculosis contains multiple versions of the accA and accD genes that encode the alpha- and beta-subunits of at least three distinct multi-functional acyl-CoA carboxylase complexes. Because of its proposed involvement in pathogenic M. tuberculosis survival, the high-resolution crystal structure of the ...

journal_title：FEBS letters

authors： Holton SJ,King-Scott S,Nasser Eddine A,Kaufmann SH,Wilmanns M

更新日期：2006-12-22 00:00:00

abstract：:Treatment of human amniotic cells (UAC) with human interferon-alpha (Hu-IFN alpha) or phorbol myristate acetate (PMA) resulted in translocation of protein kinase C (PK-C) activity from the cytosol fraction to that of the membranes. Analysis of 32P incorporation into phospholipid fractions and studies of alterations in...

journal_title：FEBS letters

authors： Premecz G,Markovits A,Bagi G,Farkas T,Földes I

更新日期：1989-06-05 00:00:00

abstract：:Lipoprotein MtsA is a critical component of MtsABC responsible for iron binding and transport in the Gram-positive bacterium Streptococcus pyogenes. The present collective experimental data establish that Fe(2+) is the primary binding ion for MtsA under optimal physiologically relevant conditions. The binding affiniti...

journal_title：FEBS letters

authors： Sun X,Ge R,Chiu JF,Sun H,He QY

更新日期：2008-04-16 00:00:00

abstract：:omega-Conotoxin MVIIC binds to P/Q-type calcium channels with high affinity and N-type channels with low affinity. To reveal the residues essential for subtype selectivity, we synthesized Ala-scanning analogs of MVIIC. Binding assays using rat cerebellar P(2) membranes suggested that Thr(11), Tyr(13) and Lys(2) are es...

journal_title：FEBS letters

authors： Sato K,Raymond C,Martin-Moutot N,Sasaki T,Ohtake A,Minami K,Van Renterghem C,Kim JI,Takahashi M,Seagar MJ

更新日期：2000-03-10 00:00:00

abstract：:In the brain inwardly rectifying potassium channel Kir7.1 subunits are predominantly expressed in the choroid plexus and meninges. To investigate this tissue-specific expression pattern, we characterized the genomic organization and the 5' proximal promoter of the rat Kir7.1 gene (Kcnj13). Starting from the major tran...

journal_title：FEBS letters

authors： Döring F,Karschin A

更新日期：2000-10-20 00:00:00

abstract：:In this paper we demonstrate that phage display technology is a suitable system for studying the interaction between the high-affinity receptor for IgE (Fc epsilon RI) and IgE. The alpha subunit extracellular domains of the human receptor were expressed on the surface of filamentous phage M13 fused to the carboxyl-ter...

journal_title：FEBS letters

authors： Scarselli E,Esposito G,Traboni C

更新日期：1993-08-23 00:00:00

abstract：:Heparin, an inhibitor of inositol trisphosphate (InsP3)-induced Ca2+ release in smooth muscle and non-muscle cells, was injected into intact frog skeletal muscle fibres. Ca2+ release from the sarcoplasmic reticulum was elicited by the normal action potential mechanism and monitored by both fura-2 fluorescence and an i...

journal_title：FEBS letters

authors： Pape PC,Konishi M,Baylor SM,Somlyo AP

更新日期：1988-08-01 00:00:00

abstract：:Red blood cells from patients homozygotes for hemoglobin S (HbS) have been studied using a computer-controlled microspectrophotometer, which allows measurements of spectra and dynamics to be undertaken in a single erythrocyte. Complete photodissociation of HbCO results in polymerization of intracellular deoxyhemoglobi...

journal_title：FEBS letters

authors： Coletta M,Alayash AI,Wilson MT,Benedetti PA,Evangelista V,Brunori M

更新日期：1988-08-15 00:00:00

abstract：:We investigated 38 cases of B-cell chronic lymphocytic leukemia (B-CLL) for the presence of non-productive rearrangements in the S(mu) regions and defined for the first time the molecular nature of these rearrangements. Southern blot analysis revealed S(mu) region rearrangements in 13 cases (34%) and polymerase chain ...

journal_title：FEBS letters

authors： Nardini E,Rizzi S,Capello D,Vitolo U,Gaidano G,Menard S,Balsari A

更新日期：2002-05-08 00:00:00

abstract：:We transfected the COS-7 cells with cDNAs encoding different human somatostatin receptor (hSSTR) subtypes, and found that hSSTR subtypes mediate not only the inhibition of forskolin-induced cAMP accumulation but also the stimulation of phospholipase C (PLC) and Ca2+ mobilization. Activation of PLC by 1 microM somatost...

journal_title：FEBS letters

authors： Akbar M,Okajima F,Tomura H,Majid MA,Yamada Y,Seino S,Kondo Y

更新日期：1994-07-11 00:00:00

abstract：:Primary cultures of rat dorsal root ganglionic (DRG) cells were stained with isoform-specific antibodies against non-muscle myosin II. Antibodies against the brain type myosin (MIIB) stained the peripheries of growth cones and non-neuronal cells. Double staining of the cells with the anti-myosin antibodies and rhodami...

journal_title：FEBS letters

authors： Cheng TP,Murakami N,Elzinga M

更新日期：1992-10-19 00:00:00

abstract：:The morula and the mesenchyme blastula nuclei contained approx. 30 nuclear proteins which were preferentially released by limited digestion with DNase I, but no proteins were released from sperm nuclei. While most of the proteins released by DNase I digestion were common to the two embryonic stages, 2 and 6 proteins w...

journal_title：FEBS letters

authors： Takahashi K

更新日期：1984-04-24 00:00:00

abstract：:The assembly of iron-sulfur (Fe/S) clusters in a living cell is mediated by a complex machinery which, in eukaryotes, is localised within mitochondria. Here, we report on a new component of this machinery, the protein Isa2p of the yeast Saccharomyces cerevisiae. The protein shares sequence similarity with yeast Isa1p ...

journal_title：FEBS letters

authors： Pelzer W,Mühlenhoff U,Diekert K,Siegmund K,Kispal G,Lill R

更新日期：2000-07-07 00:00:00

abstract：:Electron paramagnetic resonance (EPR) and near-infrared magnetic circular dichroism (MCD) have been used to identify the ligands to the cytochrome b556 component of succinate: ubiquinone oxidoreductase (succinate dehydrogenase) from Escherichia coli. The 'highly axial low spin' (HALS) EPR spectrum suggests bis(histidi...

journal_title：FEBS letters

authors： Peterson J,Vibat C,Gennis RB

更新日期：1994-11-28 00:00:00

abstract：:The C-terminal regions of Kv1 K+ channels show little conservation between isoforms except for the last four C-terminal residues, (E/L)TDV, which are well conserved from Drosophila to man. Deletions of the 4, 16, and 57 C-terminal residues of the human Kv1.5 channel did not affect whole cell current amplitude, midpoin...

journal_title：FEBS letters

authors： Uebele VN,Yeola SW,Snyders DJ,Tamkun MM

更新日期：1994-02-28 00:00:00

abstract：:Neuritic plaques are the key pathological feature of Alzheimer's disease, and amyloid beta (Abeta) peptides are major component of these plaques. In this study, we demonstrated the influence of aluminum (Al) on the Abeta peptide degradation by cathepsin D. Al did not directly affect the cathepsin D activity using smal...

journal_title：FEBS letters

authors： Sakamoto T,Saito H,Ishii K,Takahashi H,Tanabe S,Ogasawara Y

更新日期：2006-12-11 00:00:00

abstract：:Chemotaxis induction is a major effect evoked by stimulation of the chemokine receptor CXCR4 with its sole ligand CXCL12. We now report that treatment of CHP-100 human neuroepithelioma cells with the glucosylceramide synthase (GCS) inhibitor DL-threo-1-phenyl-2-hexadecanoylamino-3-pyrrolidino-1-propanol inhibits CXCR4...

journal_title：FEBS letters

authors： Limatola C,Massa V,Lauro C,Catalano M,Giovanetti A,Nuccitelli S,Spinedi A

更新日期：2007-06-12 00:00:00

abstract：:Accurate cDNA data is useful to validate gene structures in a genome. We sequenced 35189 expressed sequence tags (ESTs) obtained from the highly destructive rice blast fungus, Magnaporthe grisea. Our custom-made computational programs mapped these ESTs on the M. grisea genome sequence, and reconstructed gene structure...

journal_title：FEBS letters

authors： Numa H,Nishimura M,Tanaka T,Kanamori H,Yang CC,Matsumoto T,Nagamura Y,Itoh T

更新日期：2009-02-18 00:00:00

abstract：:In Alzheimer's disease (AD) brain, microtubule-associated protein tau is abnormally modified by hyperphosphorylation and glycosylation, and is aggregated as neurofibrillary tangles of paired helical filaments. To investigate the role of tau glycosylation in neurofibrillary pathology, we isolated various pools of tau p...

journal_title：FEBS letters

authors： Liu F,Zaidi T,Iqbal K,Grundke-Iqbal I,Merkle RK,Gong CX

更新日期：2002-02-13 00:00:00

abstract：:Bacterial cells containing the ner gene of phage Mu inserted into pBR322 express a binding activity with specificity for the left-end EcoRI.C fragment of Mu DNA. Crude extracts containing either Mu repressor or ner protein have been used to localize binding sites on TaqI subfragments of the EcoRI.C fragment. There are...

journal_title：FEBS letters

authors： Barlach S,Schumann W

更新日期：1983-06-27 00:00:00

abstract：:The heat shock factor (HSF1) is the central regulator of the heat stress (hs) response and is required for stimulating the transcription of the hs genes and consequently the expression of heat shock proteins. To promote the polymerase II-dependent transcription of the hs genes, HSF has to communicate with the basal tr...

journal_title：FEBS letters

authors： Reindl A,Schöffl F

更新日期：1998-10-09 00:00:00

abstract：:Calmodulin is shown to interact with human spectrin dimer. The binding was highly calcium-dependent and observed in two different kinds of experiments. Firstly, affinity chromatography of calmodulin on a Sepharose 4B column with immobilized spectrin, and secondly, partition in aqueous two-phase polymer systems. In the...

journal_title：FEBS letters

authors： Berglund A,Backman L,Shanbhag VP

更新日期：1984-06-25 00:00:00

abstract：:Structure/function relationships in troponin C are studied with vertebrate fast-twitch fibers by exchanging the skeletal troponin C with two bacterially synthesized recombinant proteins designed by site-directed mutagenesis of cardiac troponin C. One mutant (CBM1) contained an additional active site, by deleting Val-2...

journal_title：FEBS letters

authors： Gulati J,Babu A,Putkey JA

更新日期：1989-05-08 00:00:00

abstract：:In exocrine acinar cells a variety of neurotransmitters (e.g. acetylcholine) stimulate phosphatidylinositol 4,5-bisphosphate hydrolysis elevating intracellular calcium to activate calcium-dependent membrane currents (outward K+ and inward Cl-). This study shows that in lacrimal acinar cells extracellular application o...

journal_title：FEBS letters

authors： Sasaki T,Gallacher DV

更新日期：1990-05-07 00:00:00

abstract：:The MDM2 oncoprotein is overexpressed in many human tumors and cancers. MDM2 functions as an E3 ligase for p53 and for itself. MDM2 also interacts with the retinoblastoma protein (RB) and the transcription factor E2F1 to promote cell cycle S-phase entry. Here, we report that MDM2 protein expression is cell cycle-regul...

journal_title：FEBS letters

authors： Gu L,Ying H,Zheng H,Murray SA,Xiao ZX

更新日期：2003-06-05 00:00:00

abstract：:Sequence comparisons of 5 S and other ribosomal RNAs by segments can be useful in understanding anomalous primary and secondary structures and in assessing phylogenetic relationships. In a segmented analysis, the 5'-half of the Chlamydomonas reinhardii chloroplast 5 S ribosomal RNA is found to have a very close sequen...

journal_title：FEBS letters

authors： Delihas N

更新日期：1987-09-14 00:00:00