The MDM2 RING finger is required for cell cycle-dependent regulation of its protein expression.

abstract：:The role of protein kinase C (PKC) in cellular regulation of L-type Ca2+ channels was investigated in human umbilical vein smooth muscle. Activation of PKC, by low concentrations (< 30 nM) of 12-O-tetradecanoyl-phorbol-13-acetate (TPA) caused inhibition of Ca2+ channels, while higher concentrations of TPA (> 100 nM) e...

journal_title：FEBS letters

authors： Schuhmann K,Groschner K

更新日期：1994-03-21 00:00:00

abstract：:The temperature dependence of single-channel conductance and open probability for outer membrane protein A (OmpA) of Escherichia coli were examined in planar lipid bilayers. OmpA formed two interconvertible conductance states, small channels, 36-140 pS, between 15 and 37 degrees C, and large channels, 115-373 pS, betw...

journal_title：FEBS letters

authors： Zakharian E,Reusch RN

更新日期：2003-12-04 00:00:00

abstract：:Small organic molecules, like Congo red and lacmoid, have been shown to modulate the self-assembly of the amyloid β peptide (Aβ). Here, we show that Aβ forms NMR invisible non-toxic co-aggregates together with lacmoid as well as Congo red. We find that the interaction involves two distinct kinetic processes and at eve...

journal_title：FEBS letters

authors： Abelein A,Lang L,Lendel C,Gräslund A,Danielsson J

更新日期：2012-11-16 00:00:00

abstract：:Chemokines play a key role in modulating leukocyte functions at sites of inflammation. To assess chondrocyte contribution to the chemotactic environment of inflamed joints the intracellular content of CC and CXC chemokines was investigated. IL-8, GROalpha, MCP-1, RANTES, MIP-1alpha and MIP-1beta expression was evaluat...

journal_title：FEBS letters

authors： Borzi RM,Mazzetti I,Macor S,Silvestri T,Bassi A,Cattini L,Facchini A

更新日期：1999-07-23 00:00:00

abstract：:The effects of various local anaesthetics (LAs) on ryanodine binding of the sheep brain ryanodine receptor were tested. Tetracaine and dibucaine inhibit the binding with half-maximal inhibition (CI50) of 0.12 mM and 0.7 mM, respectively. Lidocaine and its analog QX-314, on the other hand, stimulate the binding up to 3...

journal_title：FEBS letters

authors： Martin C,Ashley R,Shoshan-Barmatz V

更新日期：1993-08-09 00:00:00

abstract：:Creutzfeldt-Jakob disease is a slow, infectious, progressive neurological disorder which results in human dementia. Synaptic membranes from various brain regions of guinea pigs infected with Creutzfeldt-Jakob disease show increased guanyl nucleotide- or 5-hydroxytryptamine-mediated activation of adenylate cyclase. Thi...

journal_title：FEBS letters

authors： Rasenick MM,Valley S,Manuelidis EE,Manuelidis L

更新日期：1986-03-17 00:00:00

abstract：:Covalent dimerization of the adrenocorticotropin fragment ACTH11-24 increases its antagonistic potency on the ACTH-induced steroidogenesis in isolated bovine fasciculata/reticularis cells by 3 orders of magnitude when the C-termini are linked via a 10 A long spacer. This strong potentiation, probably mediated by cross...

journal_title：FEBS letters

authors： Fauchère JL,Rossier M,Capponi A,Vallotton MB

更新日期：1985-04-22 00:00:00

abstract：:The binding of L-[35S]methionine in vivo labelled CryIC toxin to its receptor in brush border membrane vesicle (BBMV's) prepared from Spodoptera littoralis and Bombyx mori was studied. Both insect species were highly susceptible to the CryIC toxin in bioassays, B. mori being 7-fold more sensitive to CryIC than S. litt...

journal_title：FEBS letters

authors： Sanchis V,Chaufaux J,Pauron D

更新日期：1994-10-24 00:00:00

abstract：:Using PCR-based techniques, we have isolated and sequenced the full-length cDNAs that encode the human neuronal nAChR subunits alpha3,4,5,7 and beta2,3,4 in the neuroblastoma cell lines SH-SY5Y and/or IMR-32. The predicted nAChR beta3- and beta4-subunit proteins contain 458 and 498 amino acids, respectively. Except fo...

journal_title：FEBS letters

authors： Groot Kormelink PJ,Luyten WH

更新日期：1997-01-06 00:00:00

abstract：:We report here the regulation of the biosynthesis and the secretion of C-type natriuretic peptide (CNP) in cultured bovine chromaffin cells. The combined treatment with protein kinase A and -C activators induced a 6-fold increase of intracellular levels of CNP-(1-103). The biosynthesized CNP-(1-103) was co-released wi...

journal_title：FEBS letters

authors： Babinski K,Féthière J,Roy M,De Léan A,Ong H

更新日期：1992-11-30 00:00:00

abstract：:We have isolated a full-size cDNA coding for cardiac troponin T (cTnT) from a human adult heart library, using a slow skeletal TnT probe. This cDNA detected a 1.2 kb mRNA in fetal and post-natal human heart, the amount of which increased during ontogenic development. Interestingly, a similar transcript was coexpressed...

journal_title：FEBS letters

authors： Mesnard L,Samson F,Espinasse I,Durand J,Neveux JY,Mercadier JJ

更新日期：1993-08-09 00:00:00

abstract：:By sequence analysis we show that the U104 domain found in the UNC104 subfamily of kinesins is a forkhead homology-associated domain (FHA). A combination of limited proteolysis, mass spectroscopy, and physicochemical analysis define this domain as a genuine autonomously folding domain. Our data show that the FHA domai...

journal_title：FEBS letters

authors： Westerholm-Parvinen A,Vernos I,Serrano L

更新日期：2000-12-15 00:00:00

abstract：:The NusB protein is involved in transcriptional regulation in bacteriophage lambda. NusB binds to the RNA form of the nut site and along with N, NusA, NusE and NusG, stabilizes the RNA polymerase transcription complex and allows stable, persistent antitermination. NusB contains a 10 residue Arg-rich RNA-binding motif ...

journal_title：FEBS letters

authors： Altieri AS,Mazzulla MJ,Zhou H,Costantino N,Court DL,Byrd RA

更新日期：1997-09-29 00:00:00

abstract：:DNA synthesis at primers d(pT)n, d(pA)n, d(pC)n, and d(pG)n in the presence of corresponding complementary templates and at hetero-oligoprimers complementary to M13 phage DNA was investigated. The values of both -log Km and log Vmax increased linearly if homo-oligoprimers contained less than 10 nucleotides. The length...

journal_title：FEBS letters

authors： Nevinsky GA,Nemudraya AV,Levina AS,Khomov VV

更新日期：1989-11-20 00:00:00

abstract：:The substrate peptide bond cleaved by cathepsins B and L is determined not by the amino acid contributing the carboxyl group to this bond as in the case of serine proteases but rather by the presence of a neighboring amino acid with a large hydrophobic side chain. From a study of the inhibitory potency in a series, Cb...

journal_title：FEBS letters

authors： Kirschke H,Wikstrom P,Shaw E

更新日期：1988-02-08 00:00:00

abstract：:Subclinical inflammation is a candidate etiological factor in the pathogenesis of metabolic syndrome and in the progression of atherosclerosis. A central role for activated macrophages has been elucidated recently as important regulators of the inflammatory process in atherosclerosis. Macrophage differentiation and fu...

journal_title：FEBS letters

authors： Szanto A,Roszer T

更新日期：2008-01-09 00:00:00

abstract：:CREB binding protein (CBP) is an acetyltransferase that plays an important role in many biological processes. Here, we show that Akt phosphorylates CBP at threonine 1871 and suppresses its acetyltransferase activity by impeding the binding of CBP to histone H3, which results in a decrease in lysine K18 acetylation and...

journal_title：FEBS letters

authors： Liu Y,Xing ZB,Zhang JH,Fang Y

更新日期：2013-04-02 00:00:00

abstract：:To assure fidelity of translation, class Ia aminoacyl-tRNA synthetases (aaRSs) edit mis-aminoacylated tRNAs. Mis-attached amino acids and structural water molecules are not included simultaneously in the current crystal structures of the aaRSctRNA complexes, where the 3'-ends (adenine 76; A76) are bound to the editing...

journal_title：FEBS letters

authors： Hagiwara Y,Nureki O,Tateno M

更新日期：2009-02-18 00:00:00

abstract：:We have studied the effects of 'hydrophobic mismatch' between a poly-Leu transmembrane helix (TMH) and the ER membrane using a glycosylation mapping approach. The simplest interpretation of our results is that the lumenal end of the TMH is located deeper in the membrane for both short (negative mismatch) and long (pos...

journal_title：FEBS letters

authors： Monné M,von Heijne G

更新日期：2001-05-11 00:00:00

abstract：:Ribosomes are ribozymes exerting substrate positioning and promoting substrate-mediated catalysis. Peptide-bonds are formed within a symmetrical region, thus suggesting that ribosomes evolved by gene-fusion. Remote interactions dominate substrate positioning at stereochemistry suitable for peptide-bond formation and e...

journal_title：FEBS letters

authors： Baram D,Yonath A

更新日期：2005-02-07 00:00:00

abstract：:The p53 tumour suppressor protein is tightly controlled by the E3 ubiquitin ligase, mouse double minute 2 (MDM2), but maintains MDM2 expression as part of a negative feedback loop. We have identified the immunophilin, 25kDa FK506-binding protein (FKBP25), previously shown to be regulated by p53-mediated repression, as...

journal_title：FEBS letters

authors： Ochocka AM,Kampanis P,Nicol S,Allende-Vega N,Cox M,Marcar L,Milne D,Fuller-Pace F,Meek D

更新日期：2009-02-18 00:00:00

abstract：:Dimethylsulphoxide (DMSO) reductase from R. capsulatus contains a molybdenum-pterin cofactor at its active site. As prepared the molybdenum is in the 6+ oxidation state, devoid of EPR signals. Stepwise reduction generates an EPR signal characteristic of Mo(V) having hyperfine coupling to a single proton and integratin...

journal_title：FEBS letters

authors： Benson N,Farrar JA,McEwan AG,Thomson AJ

更新日期：1992-07-28 00:00:00

abstract：:A cDNA clone encoding cytochrome P-450(11)beta of rat adrenal has been cloned and sequenced using a bovine P-450(11)beta cDNA insert (pcP-450(11 beta)-2; (1987) J. Biochem. 102, 559-568) as a probe. The nucleotide sequence contains an open reading frame sufficient to encode the entire amino acid sequence of a P-450(11...

journal_title：FEBS letters

authors： Nonaka Y,Matsukawa N,Morohashi,Omura T,Ogihara T,Teraoka H,Okamoto M

更新日期：1989-09-11 00:00:00

abstract：:In higher plants, two types of nitrate transporters, NRT1 and NRT2, have been identified. In Arabidopsis, there are 53 NRT1 genes and 7 NRT2 genes. NRT2 are high-affinity nitrate transporters, while most members of the NRT1 family are low-affinity nitrate transporters. The exception is CHL1 (AtNRT1.1), which is a dual...

journal_title：FEBS letters

authors： Tsay YF,Chiu CC,Tsai CB,Ho CH,Hsu PK

更新日期：2007-05-25 00:00:00

abstract：:Vibrio cholerae causes the cholera disease through secretion of cholera toxin (CT), resulting in severe diarrhoea by modulation of membrane transporters in the intestinal epithelium. Genes encoding membrane-spanning transporters identified as being differentially expressed during cholera disease in a microarray screen...

journal_title：FEBS letters

authors： Flach CF,Qadri F,Bhuiyan TR,Alam NH,Jennische E,Holmgren J,Lönnroth I

更新日期：2007-07-10 00:00:00

abstract：:Jasmonic acid (JA) has been shown to be a signaling compound which elicits the production of secondary metabolites including phytoalexins in plants. It has been shown that the phytoalexin production is elicited by exogenously applied JA in rice leaves. We now show that this phytoalexin production by exogenously applie...

journal_title：FEBS letters

authors： Tamogami S,Rakwal R,Kodama O

更新日期：1997-07-21 00:00:00

abstract：:The degradation of type IX collagen, a minor collagen in cartilage, was examined by treatment with three different types of matrix metalloproteinases (MMPs) purified from the culture medium of rheumatoid synovial cells. Neither MMP-1 (collagenase) nor MMP-2 (so-called 'gelatinase') could digest type IX collagen, but M...

journal_title：FEBS letters

authors： Okada Y,Konomi H,Yada T,Kimata K,Nagase H

更新日期：1989-02-27 00:00:00

abstract：:Here we present a method to purify large amounts of highly pure and stably arrested ribosome-nascent chain complexes (RNCs) from Escherichia coli cells. It relies on the combined use of translation-arrest sequences to generate nascent polypeptides of specified length and subsequent tag purification of the RNCs. Moreov...

journal_title：FEBS letters

authors： Rutkowska A,Beerbaum M,Rajagopalan N,Fiaux J,Schmieder P,Kramer G,Oschkinat H,Bukau B

更新日期：2009-07-21 00:00:00

abstract：:The thermophilic cyanobacterial strain, PCC7518, originally identified as 'Mastigocladus laminosus HTF' does not show branchings or heterocysts. The absence of branchings supports the later assignment to the genus Chlorogloeopsis. The absence of heterocysts may be the result of a mutation because heterocysts were obse...

journal_title：FEBS letters

authors： Wilmotte A,Van der Auwera G,De Wachter R

更新日期：1993-02-08 00:00:00

abstract：:This study was undertaken to demonstrate the unique specificity of the chemokine receptor CXCR4 antagonist AMD3100. Calcium flux assays with selected chemokine/cell combinations, affording distinct chemokine receptor specificities, revealed no interaction of AMD3100 with any of the chemokine receptors CXCR1 through CX...

journal_title：FEBS letters

authors： Hatse S,Princen K,Bridger G,De Clercq E,Schols D

更新日期：2002-09-11 00:00:00