Abstract:
:The NusB protein is involved in transcriptional regulation in bacteriophage lambda. NusB binds to the RNA form of the nut site and along with N, NusA, NusE and NusG, stabilizes the RNA polymerase transcription complex and allows stable, persistent antitermination. NusB contains a 10 residue Arg-rich RNA-binding motif (ARM) at the N-terminus but is not sequentially homologous to any other proteins. In contrast to other known ARM-containing proteins, NusB forms a stable structure in solution in the absence of RNA. NMR spectroscopy was used to determine that NusB contains six alpha-helices: R10-Q21, 127-F34, V45-L65, Q79-S93, Y100-F114 and D118-L127. The structure of NusB makes it a member of a newly emerging class of alpha-helical RNA-binding proteins.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Altieri AS,Mazzulla MJ,Zhou H,Costantino N,Court DL,Byrd RAdoi
10.1016/s0014-5793(97)01128-9subject
Has Abstractpub_date
1997-09-29 00:00:00pages
221-6issue
2eissn
0014-5793issn
1873-3468pii
S0014-5793(97)01128-9journal_volume
415pub_type
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