Abstract:
:Fourier transform infrared spectroscopy is used to estimate the secondary structure of bacteriorhodopsin dissolved in chloroform-methanol (1:1 v/v), 0.1 M LiClO4. Curve-fitting of the deconvolved spectra in the amide I region shows that the total content of alpha-helices, reverse turns and beta-sheets are similar to the native state. However, the alpha II-helices, which are the major helical class in native bacteriorhodopsin, are greatly decreased in the solubilized sample. Similarly, the reverse turns and the beta-sheets are strongly altered.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Torres J,Padrós Edoi
10.1016/0014-5793(93)81331-ssubject
Has Abstractpub_date
1993-02-22 00:00:00pages
77-9issue
1eissn
0014-5793issn
1873-3468pii
0014-5793(93)81331-Sjournal_volume
318pub_type
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