Abstract:
:Two hybrid proteins between Pseudomonas aeruginosa exotoxin A (PE) and poliovirus protease 2Apro have been generated. One hybrid protein contains the poliovirus 2Apro sequence replacing the region of PE corresponding to amino acids 413-607. The other hybrid contains in addition the transforming growth factor sequence. The two hybrid proteins were efficiently synthesized in E. coli cells using the inducible pET vectors. Both hybrid toxins cleaved p220 (eIF-4 gamma) when the recombinant plasmids were transfected in COS cells infected with recombinant vaccinia virus bearing the T7 RNA polymerase gene.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Novoa I,Feduchi E,Carrasco Ldoi
10.1016/0014-5793(94)01157-5subject
Has Abstractpub_date
1994-11-21 00:00:00pages
45-8issue
1eissn
0014-5793issn
1873-3468pii
0014-5793(94)01157-5journal_volume
355pub_type
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