Abstract:
:Contact inhibition of locomotion (CIL) occurs when a cell ceases moving in the same direction following contact with another cell. Homotypic and heterotypic CIL occur between cells of the same and different types, respectively. Using Abercrombie's confronted explants assay we studied the effect of changing Rac1 or RhoA activities on heterotypic CIL between NIH3T3 and chicken heart fibroblasts. Both dominant active (L61) and dominant negative (N17) Rac1 expressed in NIH3T3 cells resulted in loss of heterotypic CIL. N17Rac1 expression caused RhoA activation. Increasing RhoA activity directly (V14RhoA) or indirectly (downregulation of N-cadherin or p120-catenin) also resulted in loss of CIL. High RhoA activity has been associated with tumour invasion and our results are consistent with loss of heterotypic CIL playing a role.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Anear E,Parish RWdoi
10.1016/j.febslet.2012.03.044subject
Has Abstractpub_date
2012-05-07 00:00:00pages
1330-5issue
9eissn
0014-5793issn
1873-3468pii
S0014-5793(12)00244-Xjournal_volume
586pub_type
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