Abstract:
:Autophagy is a bulk degradation process conserved among eukaryotes. In macro-autophagy, autophagosomes sequester cytoplasmic components and deliver their contents to lysosomes/vacuoles. Autophagosome formation requires the conjugation of Atg8, a ubiquitin-like protein, to phosphatidylethanolamine (PE). Here we report that the amino (N)-terminal region of Atg3, an E2-like enzyme for Atg8, plays a crucial role in Atg8-PE conjugation. The conjugating activities of Atg3 mutants lacking the 7 N-terminal amino acid residues or containing a Leu-to-Asp mutation at position 6 were severely impaired both in vivo and in vitro. In addition, the amino-terminal region is critical for interaction with the substrate, PE.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Hanada T,Satomi Y,Takao T,Ohsumi Ydoi
10.1016/j.febslet.2009.03.009subject
Has Abstractpub_date
2009-04-02 00:00:00pages
1078-83issue
7eissn
0014-5793issn
1873-3468pii
S0014-5793(09)00182-3journal_volume
583pub_type
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