Abstract:
:Both cisplatin and the estrogen receptor (ER) are known to bend DNA. The influence of the bending of sequences by the d(GpG)cisPt adduct binding of ER to estrogen response element (ERE)-like sequences was examined. Three ERE-like oligonucleotides with different affinities for ER and which include a GG in the linker sequence were designed in order to form a single central d(GpG)cisPt adduct. Using electrophoretic mobility shift assay and Scatchard analysis, it was shown that the presence of a single d(GpG)cisPt adduct in the linker sequence decreases the ER affinity for DNA. These results do not support a critical role of a DNA bend in the initial recognition of ERE by ER. Then, the platination of DNA outside of the ERE half-sites decreases the interaction of ER with ERE.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Massaad-Massade L,Massaad C,Legendre F,Bas V,Chottard J,Beaune P,Barouki Rdoi
10.1016/s0014-5793(99)01755-xkeywords:
subject
Has Abstractpub_date
2000-01-21 00:00:00pages
49-53issue
1eissn
0014-5793issn
1873-3468pii
S001457939901755Xjournal_volume
466pub_type
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