Abstract:
:We applied 1D homonuclear Hartmann-Hahn (1D-HOHAHA) and difference NOE experiments to determine the chemical structure of Forssman's antigen, a glycolipid purified from sheep red blood cells. The subspectra corresponding to the individual sugar components were extracted from overlapping proton resonances by selective excitation of the anomeric proton resonances, so that unambiguous assignments of the sugar proton resonances were accomplished. Then, difference NOE experiments were performed to determine the linkage of the sugar units. The present procedure was found to be useful for the structure determination of glycoconjugates and also reduces the amount of samples and machine time.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Inagaki F,Kodama C,Suzuki M,Suzuki Adoi
10.1016/0014-5793(87)81188-2subject
Has Abstractpub_date
1987-07-13 00:00:00pages
45-50issue
1eissn
0014-5793issn
1873-3468pii
0014-5793(87)81188-2journal_volume
219pub_type
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