Mapping the heparin-binding domain of boar spermadhesins.


:Boar spermadhesins are a group of seminal plasma, heparin-binding proteins which appear to be involved in sperm capacitation and gamete interaction. Using a proteolytic protection assay we have identified regions of AQN-1, AQN-3, PSP-I and AWN which remain attached to a heparin-Sepharose column following in-column digestion of bound spermadhesins with chymotrypsin and elastase. In addition, the complete amino acid sequence of spermadhesin AWN was synthesized as overlapping peptides, and their ability to bind to a heparin-Sepharose column and to inhibit the interaction of soluble heparin with purified ELISA plate-coated AWN was tested. Both approaches gave similar results and as a whole showed that different regions of AWN may converge in its tertiary structure to form a composite heparin-binding site. The conformational heparin-binding surface resides on the GFCC'C'' face of the proposed structural model for AWN and is in an opposite location to the carbohydrate-binding region of the spermadhesin.




FEBS letters


Calvete JJ,Dostàlova Z,Sanz L,Adermann K,Thole HH,Töpfer-Petersen E




Has Abstract


1996-02-05 00:00:00














  • Dimeric and tetrameric forms of catalytically active transmembrane CD38 in transfected HeLa cells.

    abstract::CD38, a type II transmembrane glycoprotein, behaves as a catalytically active transporter responsible for ectocellular generation of cyclic ADP-ribose (cADPR) from NAD+ and for subsequent influx of cADPR across membranes [Franco, L., Guida, L., Bruzzone, S., Zocchi, E., Usai, C. and De Flora, A. (1998) FASEB J. in pre...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Bruzzone S,Guida L,Franco L,Zocchi E,Corte G,De Flora A

    更新日期:1998-08-21 00:00:00

  • Characterization of Arabidopsis decapping proteins AtDCP1 and AtDCP2, which are essential for post-embryonic development.

    abstract::Although decapping is an important process in eukaryotic mRNA turnover, little is known about this process in plants. Here, we identified Arabidopsis thaliana decapping proteins AtDCP1 and AtDCP2 and showed that (I) AtDCP2 is an active decapping enzyme, (II) AtDCP1 interacts with itself, (III) AtDCP1 and AtDCP2 are lo...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Iwasaki S,Takeda A,Motose H,Watanabe Y

    更新日期:2007-05-29 00:00:00

  • Zymosan induces selective release of arachidonic acid from rabbit alveolar macrophages via stimulation of a beta-glucan receptor.

    abstract::Zymosan, which is composed primarily of alpha-mannan and beta-glucan polymers, is a well recognized activator of macrophages. The type receptor by which unopsonized zymosan induces arachidonic acid release was investigated. It was found that particulate beta-glucan and zymosan stimulated an identical dose-dependent re...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Daum T,Rohrbach MS

    更新日期:1992-09-07 00:00:00

  • Change in lactate production in Myc-transformed cells precedes apoptosis and can be inhibited by Bcl-2 overexpression.

    abstract::As a result of Myc-dependent transcription of the LDH-A gene, Myc-transformed cells (Rat1-Myc) exhibit increased lactate production rates (LPR) even under aerobic conditions (the Warburg effect). Recently, the increased susceptibility to stress-induced apoptosis associated with Myc transfection has been linked to the ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Papas KK,Sun L,Roos ES,Gounarides JS,Shapiro M,Nalin CM

    更新日期:1999-03-12 00:00:00

  • Localization of myosin IIB at the leading edge of growth cones from rat dorsal root ganglionic cells.

    abstract::Primary cultures of rat dorsal root ganglionic (DRG) cells were stained with isoform-specific antibodies against non-muscle myosin II. Antibodies against the brain type myosin (MIIB) stained the peripheries of growth cones and non-neuronal cells. Double staining of the cells with the anti-myosin antibodies and rhodami...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Cheng TP,Murakami N,Elzinga M

    更新日期:1992-10-19 00:00:00

  • Role of a serum phospholipase A1 in the phosphatidylserine-induced T cell inhibition.

    abstract::We have previously shown that unsaturated phosphatidylserines inhibit mitogen-induced T cell activation. We now report that the inhibitory action requires a protein present in bovine and human serum. Partial purification and phospholipase assay show that this protein has phospholipase A activity on phosphatidylserine ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Bellini F,Bruni A

    更新日期:1993-01-18 00:00:00

  • A role for the octameric ring protein, Translin, in mitotic cell division.

    abstract::The octameric ring protein, Translin, demonstrates marked similarities to the family of helicase enzymes regarding its quaternary organization and dimerization of subunits. Here we show that the level of Translin closely parallels the proliferative state in various cell types. Expression is periodic during the cell cy...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Ishida R,Okado H,Sato H,Shionoiri C,Aoki K,Kasai M

    更新日期:2002-08-14 00:00:00

  • Elevated citrate levels in non-alcoholic fatty liver disease: the potential of citrate to promote radical production.

    abstract::Plasma citrate levels were found to be elevated in non-alcoholic fatty liver disease (NAFLD) patients. Cellular experiments indicated that increased citrate levels might originate from an excess of fatty acids. The impact of elevated citrate levels on oxidative stress was examined. It was found that citrate stimulated...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: van de Wier B,Balk JM,Haenen GR,Giamouridis D,Bakker JA,Bast BC,den Hartog GJ,Koek GH,Bast A

    更新日期:2013-08-02 00:00:00

  • A key role of nuclear factor Y in the refeeding response of fatty acid synthase in adipocytes.

    abstract::Fatty acid synthase (Fasn) is a key component of energy metabolism that is dynamically induced by food intake. Although extensive studies have revealed a number of transcription factors involved in the fasting/refeeding transition of Fasn expression in hepatocytes, much less evidence is available for adipocytes. Using...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Nishi-Tatsumi M,Yahagi N,Takeuchi Y,Toya N,Takarada A,Murayama Y,Aita Y,Sawada Y,Piao X,Oya Y,Shikama A,Masuda Y,Kubota M,Izumida Y,Matsuzaka T,Nakagawa Y,Sekiya M,Iizuka Y,Kawakami Y,Kadowaki T,Yamada N,Shimano

    更新日期:2017-04-01 00:00:00

  • Sequence-specific binding property of Arabidopsis thaliana telomeric DNA binding protein 1 (AtTBP1).

    abstract::We have identified an Arabidopsis thaliana cDNA, designated as AtTBP1, encoding a protein with a predicted size of 70.6 kDa that specifically binds to the plant telomeric repeat sequence TTTAGGG. AtTBP1 is present as a single-copy gene in Arabidopsis genome and is expressed ubiquitously in various organs. AtTBP1 has a...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Hwang MG,Chung IK,Kang BG,Cho MH

    更新日期:2001-08-10 00:00:00

  • The RhoA transcriptional program in pre-T cells.

    abstract::The GTPase RhoA is essential for the development of pre-T cells in the thymus. To investigate the mechanisms used by RhoA to control thymocyte development we have used Affymetrix gene profiling to identify RhoA regulated genes in T cell progenitors. The data show that RhoA plays a specific and essential role in pre-T ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Mullin M,Lightfoot K,Clarke R,Miller M,Lahesmaa R,Cantrell D

    更新日期:2007-09-04 00:00:00

  • Growth factor receptors in helminth parasites: signalling and host-parasite relationships.

    abstract::Parasitic helminths remain major pathogens of both humans and animals throughout the world. The success of helminth infections depends on the capacity of the parasite to counteract host immune responses but also to exploit host-derived signal molecules for its development. Recent progress has been made in the characte...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审


    authors: Dissous C,Khayath N,Vicogne J,Capron M

    更新日期:2006-05-22 00:00:00

  • Evidence for co-regulation of Cu,Zn superoxide dismutase and metallothionein gene expression in yeast through transcriptional control by copper via the ACE 1 factor.

    abstract::Saccharomyces cerevisiae mutant strain DTY26, lacking ACE1, the protein mediator for the induction of metallothionein gene expression, is unable to increase Cu,Zn superoxide dismutase mRNA in response to copper. In the wild-type strain DTY22 transcription of both Cu,Zn superoxide dismutase and metallothionein genes is...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Carri MT,Galiazzo F,Ciriolo MR,Rotilio G

    更新日期:1991-01-28 00:00:00

  • NaCl-activated nucleoside diphosphate kinase from extremely halophilic archaeon, Halobacterium salinarum, maintains native conformation without salt.

    abstract::Enzymes from extremely halophilic archaea are readily denatured in the absence of a high salt concentration. However, we have observed here that a nucleoside diphosphate kinase prepared from Halobacterium salinarum was active and stable in the absence of salt, though it has the amino acid composition characteristic of...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Ishibashi M,Tokunaga H,Hiratsuka K,Yonezawa Y,Tsurumaru H,Arakawa T,Tokunaga M

    更新日期:2001-03-30 00:00:00

  • Identification of enzymes acting on alpha-glycated amino acids in Bacillus subtilis.

    abstract::We have characterized the Bacillus subtilis homologs of fructoselysine 6-kinase and fructoselysine-6-phosphate deglycase, two enzymes that specifically metabolize the Amadori compound fructose-epsilon-lysine in Escherichia coli. The B. subtilis enzymes also catalyzed the phosphorylation of fructosamines to fructosamin...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Wiame E,Duquenne A,Delpierre G,Van Schaftingen E

    更新日期:2004-11-19 00:00:00

  • Liposome-mediated peptide loading of MHC-DR molecules in vivo.

    abstract::Amino acid residues 3-15 of mycobacterial HSP60 define a dominant T-cell epitope for HLA-DR3+ve humans and Mamu-DR3+ve rhesus monkeys. Our results show that Mamu-DR3 molecules on PBMC can be efficiently loaded in vivo with the above-mentioned peptides when they are intravenously injected encapsulated in liposomes, but...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: t Hart BA,Elferink DG,Drijfhout JW,Storm G,van Blooijs L,Bontrop RE,de Vries RR

    更新日期:1997-06-02 00:00:00

  • Degradation of type IX collagen by matrix metalloproteinase 3 (stromelysin) from human rheumatoid synovial cells.

    abstract::The degradation of type IX collagen, a minor collagen in cartilage, was examined by treatment with three different types of matrix metalloproteinases (MMPs) purified from the culture medium of rheumatoid synovial cells. Neither MMP-1 (collagenase) nor MMP-2 (so-called 'gelatinase') could digest type IX collagen, but M...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Okada Y,Konomi H,Yada T,Kimata K,Nagase H

    更新日期:1989-02-27 00:00:00

  • Formation of 22 and 24 carbon 6-desaturated fatty acids from exogenous deuterated arachidonic acid is activated in THP-1 cells at high substrate concentrations.

    abstract::Deuterated arachidonic acid (AA, [2H8]20:4 n-6) 1-25 microM, is converted to other fatty acids, as evaluated by gas chromatography-mass spectrometry, in THP-1 cells. The major products, in the 1 to 10 microM range, are 22:4 (elongated) and 20:3 (reduced in 5). At 25 microM, 24:4, 24:5 and 22:5 accumulate, with [2H8]/[...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Caruso D,Risé P,Galella G,Regazzoni C,Toia A,Galli G,Galli C

    更新日期:1994-05-02 00:00:00

  • Amino acid sequences of cytotoxin-like basic proteins derived from cobra venoms.

    abstract::Amino acid sequences of cytotoxin-like basic proteins (CLBPs), purified from the venoms of Formosan cobra (Naja naja atra) and Indian cobra (Naja naja), were reinvestigated. The determined sequences differed from those reported previously by Takechi et al. [(1985) Biochem. Int. 11, 795-802; (1987) Biochem. Int. 14, 14...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Inoue S,Okumura K,Tsujino M,Ohkura K,Ikeda K,Takechi M,Tanaka Y,Hayashi K

    更新日期:1989-11-06 00:00:00

  • Sequence effects of single base loops in intramolecular quadruplex DNA.

    abstract::We have examined the properties of intramolecular G-quadruplexes in which the G3 tracts are separated by single base loops. The most stable complex contained 1',2'-dideoxyribose in all three loops, while loops containing T and C were slightly less stable (by about 2 degrees C). Quadruplexes containing loops with singl...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Rachwal PA,Brown T,Fox KR

    更新日期:2007-04-17 00:00:00

  • New and unexpected routes for ultrafast electron transfer in photosynthetic reaction centers.

    abstract::In photosynthetic reaction centers, the excitation with light leads to the formation of a charge separated state across the photosynthetic membrane. For the reaction center of purple non-sulphur bacteria, it was previously generally assumed that this primary charge separation could only start with the excitation of th...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审


    authors: van Brederode ME,van Grondelle R

    更新日期:1999-07-16 00:00:00

  • Perspectives on the automatic design of regulatory systems for synthetic biology.

    abstract::Automatic design is based on computational modeling and optimization methods to provide prototype designs to targeted problems in an unsupervised manner. For biological circuits, we need to produce quantitative predictions of cell behavior for a given genotype as consequence of the different molecular interactions. Au...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审


    authors: Rodrigo G,Carrera J,Landrain TE,Jaramillo A

    更新日期:2012-07-16 00:00:00

  • Gcm1, a mammalian homolog of Drosophila glial cells missing.

    abstract::Differentiation of glia (astrocytes and oligodendrocytes) in Drosophila requires the gene glial cells missing (gcm), which controls lineage determination. In the absence of gcm, neuroglia progenitors exclusively differentiate into neurons, instead of into both neurons and glia. In contrast, ectopic overexpression of g...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Altshuller Y,Copeland NG,Gilbert DJ,Jenkins NA,Frohman MA

    更新日期:1996-09-16 00:00:00

  • Inactivation of cGMP-dependent conductance of rod outer segment plasma membrane induced by cGMP.

    abstract::Integral cGMP-dependent currents as well as activity of single cGMP-activated channels in plasma membrane of rod outer segment (ROS) were recorded using the patch-clamp method. The dependence of integral currents on cGMP concentration is shown to be bell-shaped. Decrease in cGMP-dependent conductance at high cGMP conc...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Zhainazarov AB,Kolesnikov SS

    更新日期:1990-01-15 00:00:00

  • MDR-1 gene expression is a minor factor in determining the multidrug resistance phenotype of MCF7/ADR and KB-V1 cells.

    abstract::The relevance of MDR-1 gene expression to the multidrug resistance phenotype was investigated. Drug-resistant cells, KB-V1 and MCF7/ADR, constantly expressed mRNA of the MDR-1 gene and were more resistant to vinblastine and adriamycin than drug-sensitive cells, KB-3-1 and MCF7. The drug efflux rate of KB-V1 was the sa...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Kim HM,Oh GT,Hong DH,Kim MS,Kang JS,Park SM,Han SB

    更新日期:1997-07-21 00:00:00

  • A recombinant polypeptide model of the second predicted nucleotide binding fold of the cystic fibrosis transmembrane conductance regulator is a GTP-binding protein.

    abstract::Association reactions of a recombinant CFTR-NBF-2 polypeptide fused to glutathione S-transferase with guanine nucleotides were monitored quantitatively by recording the fluorescence enhancement of excited trinitrophenol (TNP)-labelled GTP after binding to NBF-2. Binding of TNP-GTP to the recombinant NBF-2 polypeptide ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Randak C,Neth P,Auerswald EA,Assfalg-Machleidt I,Roscher AA,Hadorn HB,Machleidt W

    更新日期:1996-11-25 00:00:00

  • High protein mobility in skinned rabbit muscle fibres observed by 1H NMR spectroscopy.

    abstract::1H NMR spectra of skinned rabbit muscle fibers show a group of relatively sharp resonance lines which presumably originate from highly mobile protein domains. Comparison with the spectrum of myosin subfragment 1 suggests that these signals may come at least partly from mobile regions of the myosin head. NMR could poss...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Kalbitzer HR,Schrumpf M,Wray J

    更新日期:1992-02-24 00:00:00

  • The low energy emitting states of the Lhca4 subunit of higher plant photosystem I.

    abstract::The selectively red excited emission spectrum, at room temperature, of the in vitro reconstituted Lhca4, has a pronounced non-equilibrium distribution, leading to enhanced emission from the directly excited low-energy pigments. Two different emitting forms (or states), with maximal emission at 713 and 735nm (F713 and ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Zucchelli G,Morosinotto T,Garlaschi FM,Bassi R,Jennings RC

    更新日期:2005-04-11 00:00:00

  • Rhizospheric NO interacts with the acquisition of reduced N sources by the roots of European beech (Fagus sylvatica L.).

    abstract::The gas phase of the soil plays an important role in plant growth and development. We investigated the effect of rhizospheric NO as a signalling compound for N uptake of beech roots. Following exposure to NO, ammonium and glutamine uptake into roots were determined using (15)N-labelling, and gene expression of selecte...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Simon J,Stoelken G,Rienks M,Rennenberg H

    更新日期:2009-09-03 00:00:00

  • Codon reading properties of an unmodified transfer RNA.

    abstract::We have previously shown that the Mycoplasma mycoides glycine tRNA (anticodon UCC) effectively reads the codons GGU and GGC in violation of the classic codon reading rules. We have attempted to elucidate what structural elements in this tRNA molecule confer this translational property and in the course of this investi...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Claesson C,Samuelsson T,Lustig F,Borén T

    更新日期:1990-10-29 00:00:00