Abstract:
:4-Picoloine-2,2':6',2"-terpyridine-platinum(II) is shown in a ligation assay to unwind and so intercalate into DNA. Circular dichroism is used to determine an equilibrium binding constant of approximately 2 x 10(7) M(-1) for the most stable binding mode of 4-picoline-2,2':6',2"-terpyridine-platinum(II) to poly[d(A-T)2] with a site size of about 4 base pairs, and about 1 x 10(6) M(-1) for a second binding mode with a site size of about 2 base pairs. Fluorescence spectroscopy provides further evidence for the strong equilibrium binding constant of 4-picoline-2,2':6',2"-terpyridine-platinum(II) in that it displaces ethidium bromide bound to DNA. The double positive charge on 4-picoline-2,2':6',2"-terpyridine-platinum(II), together with the intercalative binding mode is probably responsible for the large binding constant.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
McCoubrey A,Latham HC,Cook PR,Rodger A,Lowe Gdoi
10.1016/0014-5793(95)01537-xsubject
Has Abstractpub_date
1996-02-12 00:00:00pages
73-8issue
1-2eissn
0014-5793issn
1873-3468pii
0014-5793(95)01537-Xjournal_volume
380pub_type
杂志文章相关文献
FEBS LETTERS文献大全abstract::Incubation of adipocytes with 125I-insulin plus leupeptin or monensin, but not chloroquine, resulted in the appearance of a novel peak of 125I-insulin (modal density about 1.20 g/ml) on density gradient centrifugation; the appearance of the peak depended on the presence of specific insulin receptors on the cell surfac...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(85)80352-5
更新日期:1985-03-25 00:00:00
abstract::We present the three-dimensional structure of the N-terminal FK506-binding protein (FKBP)-like domain of the immunophilin FKBP42 from Arabidopsis thaliana. The data provide the structural background for the explanation of key functional properties reported previously. ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2005.12.007
更新日期:2006-01-09 00:00:00
abstract::Previously, we found that intracellular reactive oxygen species (ROS) affect photomorphogenesis in Neurospora crassa. In this study, we investigated the physiological roles of ROS in the response to light and found that the exposure of mycelia to air was important for the light-induced carotenogenesis. Mycelia treated...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2005.06.014
更新日期:2005-07-18 00:00:00
abstract::The activity of calcium, phospholipid-dependent protein kinase (PKc), which is thought to play an important role in cell proliferation, has been measured in the particulate and soluble fractions of cultured cells, under different proliferative conditions. Our results indicate that proliferating cells display higher PK...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(86)80192-2
更新日期:1986-01-20 00:00:00
abstract::The formation of coenzyme A thiol esters of cinnamic, p-coumaric, p-methoxy cinnamic, and ferulic acids was catalyzed by enzyme preparations from cell suspension cultures of leaf petioles from parsley (Petroselinum hortense Hoffm.). Of these acids, p-coumaric acid served as the most efficient substrate. Enzyme activit...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(70)80492-6
更新日期:1970-11-09 00:00:00
abstract::The mechanism of stimulation of hepatic respiration by fatty acids was studied in isolated rat hepatocytes. Stimulation of respiration by fatty acids varied from about 35% to about 105% depending on chain length. The stimulatory effect of octanoate (1 mM) or oleate (0.5 mM) was prevented by oligomycin (2 micrograms/ml...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(85)80161-7
更新日期:1985-12-02 00:00:00
abstract::Dosage compensation involves fine-tuning of gene expression at the level of entire chromosomes. The principles that assure selective targeting of the male X chromosome in Drosophila and the mechanism by which transcription levels are adjusted in a twofold range are still mysterious. We discuss the prevalent models in ...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2005.03.050
更新日期:2005-06-13 00:00:00
abstract::Coeliac disease is a chronic enteropathy caused by the ingestion of wheat gliadin and other cereal prolamines derived from rye and barley. In the present work, we investigated the mechanisms underlying altered barrier function properties exerted by gliadin-derived peptides in human Caco-2 intestinal epithelial cells. ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2005.07.066
更新日期:2005-08-29 00:00:00
abstract::Large conductance voltage-dependent and Ca(2+)-modulated K(+) channels play a crucial role in myometrium contractility. Western blots and immunocytochemistry of rat uterine sections or isolated cells show that MaxiK channel protein signals drastically decrease towards the end of pregnancy. Consistent with a transcript...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)01394-0
更新日期:1999-11-05 00:00:00
abstract::Vacuolar H(+)-PPase, a membrane bound proton-translocating pyrophosphatase found in various species including plants, some protozoan and prokaryotes, has been demonstrated to be localized to the vacuolar membrane in plants. Using a GUS reporter system and a green fluorescent protein (GFP) fusion protein, we investigat...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(00)02400-5
更新日期:2001-01-12 00:00:00
abstract::The fat mouse strain exhibits a late-onset obesity syndrome associated with a mutation in the gene encoding carboxypeptidase E (CPE). Since CPE plays a central role in the biosynthesis of a number of regulatory peptides, including gastrin, we examined the biogenesis and processing of progastrin in fat/fat mice by meas...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)01164-2
更新日期:1997-10-13 00:00:00
abstract::A cytosolic calmodulin wave has been described in the pre-replicative phase of rat liver regeneration. Here we demonstrate that this calmodulin increase is reduced by injection of cycloheximide (translation inhibitor) and actinomycin D (transcription inhibitor). In addition, we found that calmodulin mRNA levels are in...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(88)80868-8
更新日期:1988-04-25 00:00:00
abstract::FT-IR analysis shows that treatment of F1ATPase with the inhibitors DCCD and Nbf-Cl, in the presence of saturating concentrations of ADP and AMP-PNP and in the absence of Mg2+, does not modify the secondary structure of the enzyme, but significantly modifies its compactness and thermal stability, although to different...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)00816-3
更新日期:1998-08-07 00:00:00
abstract::Autophagy in the yeast is similar to that in mammalian cells. A mutant designated as apg1 (autophagy) defective in accumulation of autophagic bodies in the vacuoles was isolated by selection using a light microscope from a mutagenized proteinase-deficient strain. In the apg1 strain, which has normal vacuolar proteinas...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)80398-e
更新日期:1993-10-25 00:00:00
abstract::Rhodamine 6G (3 microM) effectively inhibited delta pH-driven ATP synthesis in Methanobacterium thermoautotrophicum while delta pNa-driven ATP synthesis was not affected by it. Rhodamine 6G inhibited Mg(2+)-stimulated ATPase activity of membrane vesicles prepared from these cells but the ATPase catalytic sector detach...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)00535-4
更新日期:1994-06-27 00:00:00
abstract::Previously, we cloned rat MRP3 as a candidate for an inducible transporter for the biliary excretion of organic anions [Hirohashi et al. (1998) Mol. Pharmacol. 53, 1068-10751. In the present study, we cloned human MRP3 (1527 amino acids) from Caco-2 cells. Human MRP3 is predominantly expressed in liver, small intestin...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)00899-0
更新日期:1998-08-14 00:00:00
abstract::P67, a new protein binding to a specific RNA probe, was purified from radish seedlings [Echeverria, M. and Lahmy, S. (1995) Nucleic Acids Res. 23, 4963-4970]. Amino acid sequence information obtained from P67 microsequencing allowed the isolation of genes encoding P67 in radish and Airabidopsis thaliana. Immunolocalis...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(00)01935-9
更新日期:2000-09-01 00:00:00
abstract::Depending on experimental conditions we have found that photo-inhibitory treatment of photosystem II (PSII) core complexes, isolated from wheat, can generate two fragments of about 23-24 kDa that contain either the C-terminal or N-terminal regions of the D1-protein. A 24 kDa C-terminal fragment appears when the water ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(92)80250-k
更新日期:1992-04-27 00:00:00
abstract::Tumour necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) is a member of the TNF family of cytokines that promotes apoptosis and NF-kappaB activation. Here we show that recombinant hu-TRAIL initiates the activation of multiple caspases, the loss of mitochondrial transmembrane potential, the cleavage of BI...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(00)01375-2
更新日期:2000-04-07 00:00:00
abstract::Insertion and deletion analyses of a protein have been less common than point mutation analyses, partly due to the lack in effective methods. This is the case with the green fluorescent protein (GFP), which is so widely applied in molecular biology and other fields. In this paper we first introduce a systematic approa...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(03)01308-5
更新日期:2003-12-18 00:00:00
abstract::Major myelin proteolipid (MMPL, also called PLP) and DM-20 are the two major intrinsic membrane proteins of CNS myelin. A specific immunological probe was obtained for MMPL by raising antibodies against the synthetic tridecapeptide 117-129 of MMPL. Antibodies against this peptide reacted with the MMPL but did not cros...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(86)80412-4
更新日期:1986-03-31 00:00:00
abstract::Adiponectin whose systemic levels are reduced in obesity-related diseases ameliorates insulin sensitivity and regulates biological processes like apoptosis, proliferation, migration and inflammation. Adiponectin binds to adiponectin receptors, AdipoR1 and AdipoR2, which are ubiquitously expressed. Clathrin-dependent e...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2010.09.035
更新日期:2010-10-22 00:00:00
abstract::Activation of the signal transducer and activator of transcription 3 (STAT3) in response to interleukin-6 (IL-6) type cytokines involves both phosphorylation of Tyr705, which enables dimerization, nuclear translocation and DNA binding, as well as ser727 phosphorylation. Here, we describe that the 65 C-terminal amino a...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(01)02354-7
更新日期:2001-04-20 00:00:00
abstract::The metabolite-modulated association of a fraction of hexokinase to mitochondria in brain is well documented, however, the involvement of other non-mitochondrial components in the binding of the hexokinase is controversial. Now we present evidence that the hexokinase binds both tubulin and microtubules in brain in vit...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(01)03136-2
更新日期:2001-11-30 00:00:00
abstract::In the yeast Saccharomyces cerevisiae the genes involved in galactose metabolism (GAL1,7,10) are transcriptionally activated more than a 1000-fold in the presence of galactose as the sole carbon source in the culture media. In the present work, we monitored the activity of the GAL10 gene promoter in different Ras-cAMP...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2007.04.018
更新日期:2007-05-15 00:00:00
abstract::Plasma citrate levels were found to be elevated in non-alcoholic fatty liver disease (NAFLD) patients. Cellular experiments indicated that increased citrate levels might originate from an excess of fatty acids. The impact of elevated citrate levels on oxidative stress was examined. It was found that citrate stimulated...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2013.06.019
更新日期:2013-08-02 00:00:00
abstract::S100 beta is a neurite extension factor and has been implicated in Alzheimer's disease and Down's syndrome. It belongs to a group of low molecular weight calcium-binding proteins containing the helix-loop-helix calcium binding motif. The structure of only one S100 protein, calbindin D9k, which has the lowest sequence ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(95)00296-l
更新日期:1995-04-17 00:00:00
abstract::RNA-binding protein kinase from amphibian oocytes modifies serine and threonine residues in the molecules of substrates and utilizes both ATP and GTP. Low concentrations of heparin inhibit protein kinase. The foregoing suggests that this enzyme is casein kinase II. It is shown that RNA-binding proteins lack active for...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(84)81363-0
更新日期:1984-05-07 00:00:00
abstract::The possible role of sphingomyelin cycle for the regulation of cell proliferation was investigated in human keratinocytes. The time-dependent breakdown of sphingomyelin was observed in the immortalized human keratinocyte cell line HaCaT as well as in primary human keratinocytes thereby providing evidence that the sphi...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(95)01421-7
更新日期:1996-01-02 00:00:00
abstract::Translocation of folded proteins by the Tat system of Escherichia coli is believed to rely on the presence of phosphatidylethanolamine (PE) and the negatively charged phospholipids cardiolipin (CL) and phosphatidylglycerol (PG). Here, we show that while PE is indeed essential for activity, the Tat system is fully func...
journal_title:FEBS letters
pub_type: 信件
doi:10.1002/1873-3468.12794
更新日期:2017-09-01 00:00:00