Acetylcholine release from guinea pig caudate slices evoked by phorbol ester and calcium.

abstract：:Activity of the plant mitochondrial alternative oxidase (AOX) can be regulated by organic acids, notably pyruvate. To date, only two well-conserved cysteine residues have been implicated in this process. We report the functional expression of two AOX isozymes (Sauromatum guttatum Sg-AOX and Arabidopsis thaliana At-AOX...

journal_title：FEBS letters

authors： Crichton PG,Affourtit C,Albury MS,Carré JE,Moore AL

更新日期：2005-01-17 00:00:00

abstract：:The fibronectin type II (FnII) domain, present in diverse vertebrate proteins, plays crucial roles in several fundamental biological processes. PDC-109, the major bovine seminal plasma protein, contains two FnII domains that bind to choline phospholipids on sperm plasma membrane and induce lipid efflux crucial for suc...

journal_title：FEBS letters

authors： Singh BP,Asthana A,Basu A,Tangirala R,Mohan Rao C,Swamy MJ

更新日期：2020-02-01 00:00:00

abstract：:Mechanisms involved in cell volume regulation are important in SS, SC cells as they might be involved in determining the extent of sickling and the generation of dense cells and irreversibly sickled cells. We have studied in these cells the response to cell swelling of the K+,Cl- transporter. We found that Hb SS, SC a...

journal_title：FEBS letters

authors： Canessa M,Spalvins A,Nagel RL

更新日期：1986-05-05 00:00:00

abstract：:In the presence of ADP, the molecular chaperones GroEL and GroES from Escherichia coli not only facilitated the refolding of various proteins, but also prevented their irreversible heat inactivation in vitro. Without nucleotides the refolding reactions were arrested by GroEL. Addition of GroES and ADP to the reaction ...

journal_title：FEBS letters

authors： Kawata Y,Nosaka K,Hongo K,Mizobata T,Nagai J

更新日期：1994-05-30 00:00:00

abstract：:The study of the three-dimensional organization of chromatin has recently gained much focus in the context of novel techniques for detecting genome-wide contacts using next-generation sequencing. These chromosome conformation capture-based methods give a deep topological insight into the architecture of the genome ins...

journal_title：FEBS letters

authors： Hofmann A,Heermann DW

更新日期：2015-10-07 00:00:00

abstract：:The majority of cases of early-onset familial Alzheimer disease are caused by mutations in the recently identified presenilin 1 (PS1) gene, located on chromosome 14. PS1, a 467 amino acid protein, is predicted to be an integral membrane protein containing seven putative transmembrane domains and a large hydrophilic lo...

journal_title：FEBS letters

authors： Mercken M,Takahashi H,Honda T,Sato K,Murayama M,Nakazato Y,Noguchi K,Imahori K,Takashima A

更新日期：1996-07-08 00:00:00

abstract：:The amino acid sequence of the eclosion hormone from the tobacco hornworm Manduca sexta has been determined, using less than 500 pmol of protein and microanalytical techniques. The protein contains 62 amino acid residues and has a molecular mass of 6813 Da. The amino-terminal sequence is similar to that of a 13-residu...

journal_title：FEBS letters

authors： Marti T,Takio K,Walsh KA,Terzi G,Truman JW

更新日期：1987-07-27 00:00:00

abstract：:Despite their differential cell tropisms, HIV-1 and HCV dramatically influence disease progression in coinfected patients. Macrophages are important target cells of HIV-1. We hypothesized that secreted HCV core protein might modulate HIV-1 replication. We demonstrate that HCV core significantly enhances HIV-1 replicat...

journal_title：FEBS letters

authors： Swaminathan G,Pascual D,Rival G,Perales-Linares R,Martin-Garcia J,Navas-Martin S

更新日期：2014-09-17 00:00:00

abstract：:We have purified two growth factors from dogfish brain and retina by using their binding affinity for heparin-Sepharose. These growth factors were eluted at 1 and 2 M NaCl similarly to those purified from bovine brain or retina. Their mitogenic activity was assayed in vitro on the same mammalian cells: bovine lens epi...

journal_title：FEBS letters

authors： Lagente O,Diry M,Courtois Y

更新日期：1986-07-07 00:00:00

abstract：:The branched-chain amino acid aminotransferase, Bcat1/Eca39, catalyzes the first step of branched-chain amino acid catabolism. Bcat1/Eca39 was originally isolated from a c-myc-induced tumor and was proven to be a direct target for c-Myc regulation. The gene is highly conserved in evolution and disruption of its yeast ...

journal_title：FEBS letters

authors： Eden A,Benvenisty N

更新日期：1999-08-27 00:00:00

abstract：:omega-Conotoxin MVIIC binds to P/Q-type calcium channels with high affinity and N-type channels with low affinity. To reveal the residues essential for subtype selectivity, we synthesized Ala-scanning analogs of MVIIC. Binding assays using rat cerebellar P(2) membranes suggested that Thr(11), Tyr(13) and Lys(2) are es...

journal_title：FEBS letters

authors： Sato K,Raymond C,Martin-Moutot N,Sasaki T,Ohtake A,Minami K,Van Renterghem C,Kim JI,Takahashi M,Seagar MJ

更新日期：2000-03-10 00:00:00

abstract：:A strain of yeast, Hansenula mrakii, secretes a toxin that kills sensitive yeasts, such as Saccharomyces cerevisiae Monoclonal antibodies raised against the toxin had both binding and neutralizing activities. The toxin in culture media was isolated by an affinity column of monoclonal antibody. The toxin is a basic pol...

journal_title：FEBS letters

authors： Yamamoto T,Imai M,Tachibana K,Mayumi M

更新日期：1986-01-20 00:00:00

abstract：:ISG20 is an interferon-induced antiviral exoribonuclease that acts on single-stranded RNA and also has minor activity towards single-stranded DNA. It belongs to the DEDDh group of RNases of the DEDD exonuclease superfamily. We have solved the crystal structure of human ISG20 complexed with two Mn2+ ions and uridine 5'...

journal_title：FEBS letters

authors： Horio T,Murai M,Inoue T,Hamasaki T,Tanaka T,Ohgi T

更新日期：2004-11-05 00:00:00

abstract：:This study was performed to prove our hypothesis that the metabolite(s) of polycyclic aromatic hydrocarbons (PAHs) caused the activation or phosphorylation of p53 via DNA damage to suppress the liver X receptor (LXR)-mediated signal transductions as a probably more direct mechanism. We found that LXR-mediated trans-ac...

journal_title：FEBS letters

authors： Iwano S,Shibahara N,Saito T,Kamataki T

更新日期：2006-02-06 00:00:00

abstract：:We have studied the incorporation of [(14)C]serine and of [(3)H]sphingosine into sphingomyelin in the presence or absence of brefeldin A (BFA) in three different cell types. Administration of BFA (1 microgram/ml) to fibroblasts for 24 h increased the incorporation of label into sphingomyelin 1.5-3 fold compared with u...

journal_title：FEBS letters

authors： Sadeghlar F,Sandhoff K,van Echten-Deckert G

更新日期：2000-07-28 00:00:00

abstract：:Misfolded or unassembled secretory proteins are retained in the endoplasmic reticulum (ER) and subsequently degraded by the cytosolic ubiquitin-proteasome system. This requires their retrograde transport from the ER lumen into the cytosol, which is mediated by the Sec61 translocon. It had remained a mystery whether ER...

journal_title：FEBS letters

authors： Plemper RK,Deak PM,Otto RT,Wolf DH

更新日期：1999-01-29 00:00:00

abstract：:Pin2/TRF1 was independently identified as a telomeric DNA-binding protein (TRF1) that regulates telomere length, and as a protein (Pin2) that can bind the mitotic kinase NIMA and suppress its lethal phenotype. We have previously demonstrated that Pin2/TRF1 levels are cell cycle-regulated and its overexpression induces...

journal_title：FEBS letters

authors： Nakamura M,Zhou XZ,Kishi S,Lu KP

更新日期：2002-03-13 00:00:00

abstract：:cDNAs encoding the isoform I alpha of the cGMP-dependent protein kinase were isolated from a bovine trachea smooth muscle cDNA library constructed in lambda gt10. The deduced protein sequence is identical with the protein sequence obtained by Edman degradation of the bovine lung enzyme [(1984) Biochemistry 23, 4207-42...

journal_title：FEBS letters

authors： Wernet W,Flockerzi V,Hofmann F

更新日期：1989-07-17 00:00:00

abstract：:Sequence homology was found by computer analysis between potato spindle tuber viroid (PSTV) RNA and U3B snRNA of Novikoff hepatoma cells. This homology is colinear in arrangement, extends in length to 81% of the entire U3B snRNA molecule and is involved in the PSTV molecule unique sites which, if depicted in terms of ...

journal_title：FEBS letters

authors： Kiss T,Pósfai J,Solymosy F

更新日期：1983-11-14 00:00:00

abstract：:Only recently have products of the enzymatic breakdown of the chlorophylls been characterized for the first time. All catabolites isolated until now from a chlorophyte and from angiosperms have in common the position at which the primary oxygenative ring cleavage occurs, yielding linear tetrapyrroles (19-formyl-1[21H,...

journal_title：FEBS letters

authors： Curty C,Engel N,Gossauer A

更新日期：1995-05-01 00:00:00

abstract：:CD133 is a widely used cell surface marker of cancer stem cells that plays an important role in tumor initiation and metastasis. Increasing evidence shows that CD133 is secreted to the extracellular space. However, the underlying mechanisms of CD133 secretion remain largely unknown. In this study, we report that secre...

journal_title：FEBS letters

authors： Li Y,Shi D,Yang F,Chen X,Xing Y,Liang Z,Zhuang J,Liu W,Gong Y,Jiang J,Wei Y

更新日期：2019-04-01 00:00:00

abstract：:In this study we analyzed the interaction of prion protein PrP(C) with components of glycosphingolipid-enriched microdomains in lymphoblastoid T cells. PrP(C) was distributed in small clusters on the plasma membrane, as revealed by immunoelectron microscopy. PrP(C) is present in microdomains, since it coimmunoprecipit...

journal_title：FEBS letters

authors： Mattei V,Garofalo T,Misasi R,Circella A,Manganelli V,Lucania G,Pavan A,Sorice M

更新日期：2004-02-27 00:00:00

abstract：:The cerebral deposition of 40-42 residue amyloid beta-protein (Abeta) is a characteristic of Alzheimer's disease. Cathepsin D is possibly involved in the intracellular clearance of Abeta (Hamazaki, H. (1996) FEBS Lett., in press). The present work shows that cathepsin D hydrolyzes wild-type Abeta 20 times faster than ...

journal_title：FEBS letters

authors： Hamazaki H

更新日期：1996-11-18 00:00:00

abstract：:The effective synthesis of the envelope antigen F1 of Y. pestis in E. coli HB101 is mediated by the expression of the caf1M gene. This gene was sequenced, and the protein encoded was found to have a significant homology with the chaperone protein PapD of uropathogenic E. coli. The data presented allow one to suppose C...

journal_title：FEBS letters

authors： Galyov EE,Karlishev AV,Chernovskaya TV,Dolgikh DA,Smirnov OYu,Volkovoy KI,Abramov VM,Zav'yalov VP

更新日期：1991-07-29 00:00:00

abstract：:We have studied whether the cooperative insecticidal effect of certain scorpion toxin pairs, namely either a combination of excitatory and depressant, or alpha and depressant scorpion toxins, would improve the efficacy of Autographa californica nucleopolyhedrovirus (AcMNPV) over a virus expressing only a single toxin,...

journal_title：FEBS letters

authors： Regev A,Rivkin H,Inceoglu B,Gershburg E,Hammock BD,Gurevitz M,Chejanovsky N

更新日期：2003-02-27 00:00:00

abstract：:Ribonuclease T1 can be unfolded and refolded without forming noticeable amounts of aggregates allowing to characterise the dimensions of a protein in different denatured states in terms of the Stokes radius RS. Upon thermal unfolding RS increases from 1.74 nm at 20 degrees C to 2.14 nm at 60 degrees C. By contrast, RS...

journal_title：FEBS letters

authors： Gast K,Zirwer D,Damaschun H,Hahn U,Müller-Frohne M,Wirth M,Damaschun G

更新日期：1997-02-24 00:00:00

abstract：:Here we summarize evidence for non-equivalence of two structurally similar active sites in transketolase and other thiamine-dependent enzymes. This non-equivalence takes place when the enzymes interact with various ligands (inhibitors, cations, coenzyme and substrates). Data on different strains in the structure of th...

journal_title：FEBS letters

authors： Kovina MV,Kochetov GA

更新日期：1998-11-27 00:00:00

abstract：:We counted base transitions (Ts) and transversions (Tv) in mutations among various types of hepatitis C virus (HCV) sequences. The Ts/Tv ratio was useful for distinguishing the (geno)type of the compared HCV sequences, since the ratio was 4.0 or more among sequences of the same type, while it was less than 2.0 among s...

journal_title：FEBS letters

authors： Tanaka T,Kato N,Hijikata M,Shimotohno K

更新日期：1993-01-04 00:00:00

abstract：:Crk and CrkL adaptors play essential neuronal positioning roles downstream of Reelin-induced Dab1 tyrosine phosphorylation. Recently we identified Cin85 to be a CrkL-SH3 binding partner from embryonic murine brain while others found Cin85 binds directly to Dab1. Here using mass spectrometry, biochemical and mutational...

journal_title：FEBS letters

authors： Bior BK,Ballif BA

更新日期：2013-01-04 00:00:00

abstract：:Formation of tubulin/Na(+),K(+)-ATPase (NKA) complex in erythrocytes of hypertensive subjects results in a 50% reduction in NKA activity. We demonstrate here that detyrosinated tubulin, which is increased in hypertensive erythrocytes membranes, enhances the inhibitory effect of acetylated tubulin on NKA activity. More...

journal_title：FEBS letters

authors： Amaiden MR,Santander VS,Monesterolo NE,Nigra AD,Rivelli JF,Campetelli AN,Pie J,Casale CH

更新日期：2015-01-30 00:00:00