Re-entering the translocon from the lumenal side of the endoplasmic reticulum. Studies on mutated carboxypeptidase yscY species.

abstract：:HMr alpha 2CPI was found to be an inhibitor of human liver cathepsin H by the measurement of the dissociation constant (Ki), the association rate constant (k1) and the dissociation rate constant (k-1) between the enzyme and the inhibitor. These data suggest that this protein-proteinase inhibitor can play a physiologic...

journal_title：FEBS letters

authors： Pagano M,Engler R

更新日期：1984-01-23 00:00:00

abstract：:Fluorescence techniques were used to study conformational changes that occur in inactive E. coli 30 S ribosomal subunits during activation by heating in 12 mM Mg2+. Activation is associated with movement of a fluorophore on the 3'-end of 16 S RNA into a less polar environment and towards a probe on the cysteine thiol ...

journal_title：FEBS letters

authors： Odom OW,Stöffler G,Hardesty B

更新日期：1984-07-23 00:00:00

abstract：:Bcl-xL, a close homolog of Bcl2, is an important regulator of apoptosis and is overexpressed in human cancer. Phosphorylation of Bcl-xL can be induced by microtubule-damaging drugs such as taxol or 2-methoxyestradiol (2-ME). By site-directed mutagenesis studies, we have identified that serine 62 is the necessary site ...

journal_title：FEBS letters

authors： Basu A,Haldar S

更新日期：2003-03-13 00:00:00

abstract：:Increased expression of low voltage-activated, T-type Ca(2+) channels has been correlated with a variety of cellular events including cell proliferation and cell cycle kinetics. The recent cloning of three genes encoding T-type alpha(1) subunits, alpha(1G), alpha(1H) and alpha(1I), now allows direct assessment of thei...

journal_title：FEBS letters

authors： Chemin J,Monteil A,Briquaire C,Richard S,Perez-Reyes E,Nargeot J,Lory P

更新日期：2000-07-28 00:00:00

abstract：:The 42 kDa precursor of surfactant protein B generates the 79 residue mature SP-B polypeptide, which belongs to the family of saposin-like proteins and has unique functional roles in pulmonary surfactant. From sequence comparisons it has been suggested that proSP-B, in addition to SP-B, contains two saposin-like domai...

journal_title：FEBS letters

authors： Zaltash S,Johansson J

更新日期：1998-02-13 00:00:00

abstract：:Proteasomes were purified from human hepatoma tissues, and their sensitivities to Na+ and K+ were examined. At concentrations of 10 mM or more, these cations were found to inhibit completely polylysine-activated casein degradation by the purified proteasomes. They also strongly inhibited the hydrolyses of peptides, al...

journal_title：FEBS letters

authors： Seol JH,Park SC,Ha DB,Chung CH,Tanaka K,Ichihara A

更新日期：1989-04-24 00:00:00

abstract：:Using the yeast two hybrid system and overlay assays we identified a putative rholrac effector, citron, which interacts with the GTP-bound forms of rho and rac1, but not with cdc42. Extensive homologies to known proteins were not observed. This 183 kDa protein contains a C6H2 zinc finger, a PH domain, and a long coile...

journal_title：FEBS letters

authors： Madaule P,Furuyashiki T,Reid T,Ishizaki T,Watanabe G,Morii N,Narumiya S

更新日期：1995-12-18 00:00:00

abstract：:Guluronate and mannuronate oligomers with various degree of polymerization were prepared from polyguluronate (PG) and polymannuronate (PM) with an alginate lyase from a Pseudoalteromonas sp., and their activities to induce cytokine secretion from mouse macrophage cell line RAW264.7 cells were examined. Enzymatically d...

journal_title：FEBS letters

authors： Iwamoto M,Kurachi M,Nakashima T,Kim D,Yamaguchi K,Oda T,Iwamoto Y,Muramatsu T

更新日期：2005-08-15 00:00:00

abstract：:The phosphatidylinositol 3-kinase/Akt pathway is responsible for key aspects of tumor progression, and is frequently hyperactivated in cancer. We have recently identified palladin, an actin-bundling protein that functions to control the actin cytoskeleton, as an Akt1-specific substrate that inhibits breast cancer cell...

journal_title：FEBS letters

authors： Chin YR,Toker A

更新日期：2010-12-01 00:00:00

abstract：:Phospholipase A2 (PLA2) from human pancreas, designated hPLA2-I, functions as a digestive enzyme. Interestingly, the present study demonstrated that the mature form of hPLA2-I stimulated the growth of a human pancreatic cancer cell line MIAPaCa-2, whereas the pro-form was ineffective. PLA2s from Laticauda semifasciata...

journal_title：FEBS letters

authors： Hanada K,Kinoshita E,Itoh M,Hirata M,Kajiyama G,Sugiyama M

更新日期：1995-10-02 00:00:00

abstract：:P(2)Y(12) receptor is a G(i)-coupled adenosine diphosphate (ADP) receptor with a critical role in platelet aggregation. It contains two potential N-linked glycosylation sites at its extra cellular amino-terminus, which may modulate its activity. Studies of both tunicamycin treatment and site-directed mutagenesis have ...

journal_title：FEBS letters

authors： Zhong X,Kriz R,Seehra J,Kumar R

更新日期：2004-03-26 00:00:00

abstract：:The early embryonic development of Xenopus is mainly governed by post-transcriptional regulations until the mid-blastula transition. In this report, we present evidence demonstrating that fertilization of Xenopus eggs triggers a complete translational arrest of mRNAs containing UA-rich elements in their 3'-untranslate...

journal_title：FEBS letters

authors： Marinx O,Bertrand S,Karsenti E,Huez G,Kruys V

更新日期：1994-05-30 00:00:00

abstract：:p38, which has been suggested to be a scaffold protein for the assembly of a macromolecular tRNA synthetase complex, contains a leucine zipper-like motif. To understand the importance of the leucine zipper-like motif of p38 (p38LZ) in macromolecular assembly, the p38LZ solution structure was investigated by circular d...

journal_title：FEBS letters

authors： Ahn HC,Kim S,Lee BJ

更新日期：2003-05-08 00:00:00

abstract：:Mutation of Thr90 to Ala has a profound effect on bacteriorhodopsin properties. T90A shows about 20% of the proton pumping efficiency of wild type, once reconstituted into liposomes. Mutation of Thr90 influences greatly the Schiff base/Asp85 environment, as demonstrated by altered lambda(max) of 555 nm and pK(a) of As...

journal_title：FEBS letters

authors： Perálvarez A,Barnadas R,Sabés M,Querol E,Padrós E

更新日期：2001-11-23 00:00:00

abstract：:Messenger RNA from intact rat pancreatic islets, or from transformed hamster beta (HIT) cells, hybridized with the cDNA probe for type I (but not type II) phospholipase A2. The levels of phospholipase A2 mRNA increased in islets from fasted rats; they decreased in islets cultured in a high glucose concentration (contr...

journal_title：FEBS letters

authors： Metz S,Holmes D,Robertson RP,Leitner W,Draznin B

更新日期：1991-12-16 00:00:00

abstract：:The covalent conjugation of oligonucleotides to antibody Fab' fragments was optimized by using oligonucleotides modified with a hexaethylene linker arm bearing three amino groups. One oligonucleotide was coupled to antibody of one specificity and a complementary oligonucleotide to antibody of a second specificity. The...

journal_title：FEBS letters

authors： Chaudri ZN,Bartlet-Jones M,Panayotou G,Klonisch T,Roitt IM,Lund T,Delves PJ

更新日期：1999-04-30 00:00:00

abstract：:The hemoregulatory peptide PyroGlu-Glu-Asp-Cys-Lys (HP5b), which inhibits myelopoietic colony formation in vitro, is shown to be a sequence motif which is also part of the effector domain of Gi alpha proteins. Out of 8 synthetic peptides with sequence variations of HP5b, those with the closest similarity to the Gi alp...

journal_title：FEBS letters

authors： Laerum OD,Frostad S,Tøn HI,Kamp D

更新日期：1990-08-20 00:00:00

abstract：:The O-chain polysaccharide (O-PS) of Bordetella avium was isolated from the lipopolysaccharide by mild acid hydrolysis to remove the lipid A, followed by hydrofluorolysis to remove the lipopolysaccharide core oligosaccharide leaving a residual O-PS for structural analysis. High resolution (1)H and (13)C NMR and MALDI ...

journal_title：FEBS letters

authors： Larocque S,Brisson JR,Thérisod H,Perry MB,Caroff M

更新日期：2003-01-30 00:00:00

abstract：:The influence of the esterification of the 3'(2') hydroxyl group of tRNA on the hydrolysis by venom phosphodiesterase (Crotalus adamanteus) was studied. It was shown that this esterification does not change the rate of hydrolysis. At pH 7-8, no significant differences were observed either when the alpha amino group of...

journal_title：FEBS letters

authors： Yot P,Gueguen P,Chapeville F

更新日期：1968-08-01 00:00:00

abstract：:Microsomal membranes from the slow-growing Morris hepatoma 9618A catalyze, in the presence of t-butyl hydroperoxide, lower rates of lipid peroxidation than rat liver microsomes. The cytochrome P-450 content of hepatoma microsomes is about 40% that of the liver. SKF 525-A, an inhibitor of mixed-function oxidase, produc...

journal_title：FEBS letters

authors： Borrello S,Galeotti T,Palombini G,Minotti G

更新日期：1986-12-15 00:00:00

abstract：:Buthionine sulfoximine (BSO) induces decreased glutathione (GSH) and trypanothione [T(SH)2 ] pools in trypanosomatids, presumably because only gamma-glutamylcysteine synthetase (γECS) is blocked. However, some BSO effects cannot be explained by exclusive γECS inhibition; therefore, its effect on the T(SH)2 metabolism ...

journal_title：FEBS letters

authors： Vázquez C,Mejia-Tlachi M,González-Chávez Z,Silva A,Rodríguez-Zavala JS,Moreno-Sánchez R,Saavedra E

更新日期：2017-12-01 00:00:00

abstract：:A new assay for the calcium channel activity of annexin V was developed. The calcium-sensitive fluorescence indicator, fura-2, was incorporated into large unilamellar vesicles (LUV). After establishing a calcium gradient across the liposomal membranes, native or mutated annexin V was added. The resulting calcium influ...

journal_title：FEBS letters

authors： Berendes R,Burger A,Voges D,Demange P,Huber R

更新日期：1993-02-08 00:00:00

abstract：:Integral cGMP-dependent currents as well as activity of single cGMP-activated channels in plasma membrane of rod outer segment (ROS) were recorded using the patch-clamp method. The dependence of integral currents on cGMP concentration is shown to be bell-shaped. Decrease in cGMP-dependent conductance at high cGMP conc...

journal_title：FEBS letters

authors： Zhainazarov AB,Kolesnikov SS

更新日期：1990-01-15 00:00:00

abstract：:The in vivo toxicity of vanadium(V) has been found to correlate with the depletion of cellular glutathione and related non-protein thiols. With a view to understanding the mechanism for this observation, we have investigated the oxidation of glutathione, cysteine N-acetylcysteine and penicillamine by vanadium(V), usin...

journal_title：FEBS letters

authors： Shi XL,Sun XY,Dalal NS

更新日期：1990-10-01 00:00:00

abstract：:Nuclear magnetic resonance (NMR) spectroscopy was used to determine the secondary structure and the three-dimensional polypeptide backbone fold of the human sterol carrier protein 2 (hSCP2), which is a basic protein with 123 residues believed to participate in the intracellular transport of cholesterol and various oth...

journal_title：FEBS letters

authors： Szyperski T,Scheek S,Johansson J,Assmann G,Seedorf U,Wüthrich K

更新日期：1993-11-29 00:00:00

abstract：:We have previously reported that Monad, a novel WD40 repeat protein, potentiates apoptosis induced by tumor necrosis factor-alpha(TNF-alpha) and cycloheximide (CHX). By affinity purification and mass spectrometry, we identified RNA polymerase II-associated protein 3 (RPAP3) as a binding protein of Monad. Overexpressio...

journal_title：FEBS letters

authors： Itsuki Y,Saeki M,Nakahara H,Egusa H,Irie Y,Terao Y,Kawabata S,Yatani H,Kamisaki Y

更新日期：2008-07-09 00:00:00

abstract：:Antibodies were raised, in rabbits, against an arachidonate- and U46619-binding protein purified from calf platelets. Spectral measurements and immunodiffusion experiments were employed to follow conformational responses of the protein in relation to platelet activation. Upon treatment with the platelet agonists, arac...

journal_title：FEBS letters

authors： Krishnan LK,Jamaluddin MP

更新日期：1987-02-23 00:00:00

abstract：:A Bacillus subtilis strain deficient in seven extracellular proteases was used to produce human mast cell chymase and is a viable expression system for serine proteases and other classes of proteins. Chymase is produced at 0.3-0.5 mg/l and is purified by three chromatography steps. Two crystal forms of PMSF-treated ch...

journal_title：FEBS letters

authors： McGrath ME,Osawa AE,Barnes MG,Clark JM,Mortara KD,Schmidt BF

更新日期：1997-08-25 00:00:00

abstract：:The neopterin derivatives, neopterin and 7,8-dihydroneopterin, modulate the cellular oxidant-antioxidant balance as well as the expression of the inducible nitric oxide synthase (iNOS) gene. Since apoptosis can be induced by reactive oxygen intermediates and nitric oxide (NO) we investigated whether these neopterin de...

journal_title：FEBS letters

authors： Schobersberger W,Hoffmann G,Hobisch-Hagen P,Bock G,Volkl H,Baier-Bitterlich G,Wirleitner B,Wachter H,Fuchs D

更新日期：1996-11-18 00:00:00

abstract：:N-glycosidically-linked glycans released by hydrazinolysis of human factor VIII/von Willebrand factor (FVIII/vWf) were separated by high-voltage electrophoresis. Five fractions were obtained, one of them representing 60% of the total amount of the N-glycosidically-linked glycans of FVIII/vWf. On the basis of the carbo...

journal_title：FEBS letters

authors： Debeire P,Montreuil J,Samor B,Mazurier C,Goudemand M,van Halbeek H,Vliegenthart JF

更新日期：1983-01-10 00:00:00