Fertilization of Xenopus eggs imposes a complete translational arrest of mRNAs containing 3'UUAUUUAU elements.

abstract：:Treatment of yeast phosphoglycerate kinase (PGK) with trypsin results in a fourfold increase in the Vmax of this enzyme, without affecting the Km. This activation is shown to be due to the removal of the C-terminal lysine residue. The C-terminal sequence folds back over the N-terminal domain and contacts the extreme N...

journal_title：FEBS letters

authors： Adams B,Fowler R,Hudson M,Pain RH

更新日期：1996-04-29 00:00:00

abstract：:Kaposi's sarcoma-associated herpesvirus (KSHV) ORF57 plays a pivotal role in mediating the nuclear export of KSHV intronless transcripts. We demonstrate that ORF57 localises and dynamically trafficks through the nucleolus. To assess if nucleolar shuttling of ORF57 is important for the export of intronless viral mRNA, ...

journal_title：FEBS letters

authors： Boyne JR,Whitehouse A

更新日期：2009-11-19 00:00:00

abstract：:Mitogen-activated protein kinase (MAPK) cascades play an important role in mediating stress responses in plants. In Arabidopsis, 20 MAPKs have been identified and classified into four major groups (A-D). Little is known about the role of group C MAPKs. We have studied the activation of Arabidopsis subgroup C1 MAPKs (A...

journal_title：FEBS letters

authors： Ortiz-Masia D,Perez-Amador MA,Carbonell J,Marcote MJ

更新日期：2007-05-01 00:00:00

abstract：:The white-rot fungus Phanerochaete chrysosporium has two intracellular beta-glucosidases (BGL1A and BGL1B) belonging to glycoside hydrolase (GH) family 1. BGL1B effectively hydrolyzes cellobiose and cellobionolactone, but BGL1A does not. We have determined the crystal structure of BGL1A in substrate-free and gluconola...

journal_title：FEBS letters

authors： Nijikken Y,Tsukada T,Igarashi K,Samejima M,Wakagi T,Shoun H,Fushinobu S

更新日期：2007-04-03 00:00:00

abstract：:The ADP(Mg2+)-deactivated oligomycin-sensitive F1-F0 ATPase of coupled submitochondrial particles treated with the substoichiometric amount of oligomycin was studied to test whether ATP synthesis and hydrolysis proceed in either direction through the same intermediates. The initial rates of ATP hydrolysis, oxidative p...

journal_title：FEBS letters

authors： Syroeshkin AV,Vasilyeva EA,Vinogradov AD

更新日期：1995-06-05 00:00:00

abstract：:Thimet oligopeptidase (EC 3.4.24.15; TOP) is a Zn(II) endopeptidase implicated in physiological regulation of processes involving neuropeptides. The present study clarifies the active site structure and mechanism of catalysis of TOP. The enzyme exhibited a bell-shaped pH dependence of activity having an acidic limb du...

journal_title：FEBS letters

authors： Sigman JA,Edwards SR,Pabon A,Glucksman MJ,Wolfson AJ

更新日期：2003-06-19 00:00:00

abstract：:We recently purified two closely related 33 kDa proteins from rat hepatic cytosol, designated bile acid binder I and II, which selectively bind bile acids with comparable affinity as glutathione S-transferase B. This work has now been extended to human liver in which we have identified a similar cytosolic binding acti...

journal_title：FEBS letters

authors： Stolz A,Sugiyama Y,Kuhlenkamp J,Kaplowitz N

更新日期：1984-11-05 00:00:00

abstract：:A specific b subunit arginine, b(Arg-36) in Escherichia coli, displays evolutionary conservation among bacterial F1F0 ATP synthases. Site-directed mutagenesis was used to generate a collection of mutations affecting b(Arg-36). The phenotype differed depending upon the substitution, and the b(Arg-36-Glu) and b(Arg-36-I...

journal_title：FEBS letters

authors： Caviston TL,Ketchum CJ,Sorgen PL,Nakamoto RK,Cain BD

更新日期：1998-06-12 00:00:00

abstract：:Xanthine oxidase was decreased 2- to 10-fold in all examined rat hepatomas irrespective of the malignancy; growth rate and degrees of histological differentiation of the neoplasms. The affinity to substrate (KM=6-8 muM) and the pH optimum (8.0) of the liver and hepatoma enzymes were the same. The reprogramming of gene...

journal_title：FEBS letters

authors： Prajda N,Weber G

更新日期：1975-11-15 00:00:00

abstract：:The amino acid sequence and part of the DNA sequence of a glutamic acid-specific serine protease from Streptomyces griseus is reported. This protease is shown to be homologous with other serine proteases. An improved purification protocol for this enzyme is described. ...

journal_title：FEBS letters

authors： Svendsen I,Jensen MR,Breddam K

更新日期：1991-11-04 00:00:00

abstract：:FabH (beta-ketoacyl-acyl carrier protein synthase III) is unique in that it initiates fatty acid biosynthesis, is inhibited by long-chain fatty acids providing means for feedback control of the process, and dictates the fatty acid profile of the organism by virtue of its substrate specificity. We report the crystal st...

journal_title：FEBS letters

authors： Gajiwala KS,Margosiak S,Lu J,Cortez J,Su Y,Nie Z,Appelt K

更新日期：2009-09-03 00:00:00

abstract：:Detailed structural models of di-cluster seven-iron ferredoxins constitute a valuable resource for folding and stability studies relating the metal cofactors' role in protein stability. The here reported, hemihedric twinned crystal structure at 2.0 A resolution from Acidianus ambivalens ferredoxin, shows an integral 1...

journal_title：FEBS letters

authors： Frazão C,Aragão D,Coelho R,Leal SS,Gomes CM,Teixeira M,Carrondo MA

更新日期：2008-03-05 00:00:00

abstract：:CD38, a type II transmembrane glycoprotein, behaves as a catalytically active transporter responsible for ectocellular generation of cyclic ADP-ribose (cADPR) from NAD+ and for subsequent influx of cADPR across membranes [Franco, L., Guida, L., Bruzzone, S., Zocchi, E., Usai, C. and De Flora, A. (1998) FASEB J. in pre...

journal_title：FEBS letters

authors： Bruzzone S,Guida L,Franco L,Zocchi E,Corte G,De Flora A

更新日期：1998-08-21 00:00:00

abstract：:We report isolation of a murine gene, NPYR-D, which predicts an intronless novel G protein-coupled receptor of 375 amino acids. Percent identities of NPYR-D to the clone Y1, Y2, rat Y4/PP1 and human Y4/PP1 receptors are 45, 32, 92 and 76, respectively. Southern blots indicate that NPYR-D and human Y4/PP1 receptor gene...

journal_title：FEBS letters

authors： Gregor P,Millham ML,Feng Y,DeCarr LB,McCaleb ML,Cornfield LJ

更新日期：1996-02-26 00:00:00

abstract：:A 15-ns molecular dynamics simulation of the fully hydrated liquid-crystalline dimyristoylphosphatidylcholine-cholesterol (DMPC-Chol) bilayer containing approximately 22 mol% Chol was carried out. The generated trajectory was analysed to investigate the mechanism of the Chol condensing effect on DMPC hydrocarbon chain...

journal_title：FEBS letters

authors： Róg T,Pasenkiewicz-Gierula M

更新日期：2001-07-27 00:00:00

abstract：:DNA-dependent RNA polymerase from blood forms and culture forms of the parasitic protozoan Trypanosoma brucei was resolved into multiple peaks of activity by DEAE-Sephadex chromatography. The enzyme from the two forms was found to be different by several criteria, suggesting that it is subject to developmental control...

journal_title：FEBS letters

authors： Tittawella I

更新日期：1988-01-25 00:00:00

abstract：:The bchM gene of Rhodobacter sphaeroides has been sequenced and then overexpressed in E. coli producing a protein of M(r) approximately. 27,500. Cell-free extracts of the transformed E. coli strain are able to methylate added Mg protoporphyrin, resulting in the formation of Mg protoporphyrin monomethyl ester. The iden...

journal_title：FEBS letters

authors： Gibson LC,Hunter CN

更新日期：1994-09-26 00:00:00

abstract：:A synthetic form of human pancreatic growth hormone releasing factor (GRF-44-NH2) was shown to be a potent stimulator of growth hormone (GH) secretion and cellular cyclic AMP levels in cultured sheep pituitary cells. A small dose-dependent stimulation of prolactin secretion was also observed. Somatostatin (0.5 microM)...

journal_title：FEBS letters

authors： Law GJ,Ray KP,Wallis M

更新日期：1984-01-23 00:00:00

abstract：:Inositol 1,4,5-trisphosphate (1,4,5-InsP3) was perfused into rat dorsal root ganglion (DRG) neurons by whole-cell patch-clamp electrodes, while measuring the membrane potential. This operation evoked a transient (2-3 min) membrane hyperpolarization of about -15 mV (from -42 mV) followed by a depolarization. The membra...

journal_title：FEBS letters

authors： Wang Z,Ypey DL,Van Duijn B

更新日期：1992-06-15 00:00:00

abstract：:Activation of AMP-activated protein kinase (AMPK) has been viewed as an important target for the treatment of insulin resistance. Here, by proteomic analysis, we found that expression of heat shock protein beta-1 (HSPB1) was induced by the AMP analog 5-aminoimidazole-4-carboxamide 1-β-D-ribofuranoside in palmitate-ind...

journal_title：FEBS letters

authors： Yuan H,Wang T,Niu Y,Liu X,Fu L

更新日期：2017-01-01 00:00:00

abstract：:The fidelity of tRNA aminoacylation is dependent in part on amino acid editing mechanisms. A hydrolytic activity that clears mischarged tRNAs typically resides in an active site on the tRNA synthetase that is distinct from its synthetic aminoacylation active site. A second pre-transfer editing pathway that hydrolyzes ...

journal_title：FEBS letters

authors： Martinis SA,Boniecki MT

更新日期：2010-01-21 00:00:00

abstract：:The archaeal exosome, a protein complex responsible for phosphorolytic degradation and tailing of RNA, has an RNA-binding platform containing Rrp4, Csl4, and DnaG. Aiming to detect novel interaction partners of the exosome, we copurified Nop5, which is a part of an rRNA methylating ribonucleoprotein complex, with the ...

journal_title：FEBS letters

authors： Gauernack AS,Lassek C,Hou L,Dzieciolowski J,Evguenieva-Hackenberg E,Klug G

更新日期：2017-12-01 00:00:00

abstract：:6-Photocholesterol, a new photoactivatable analog of cholesterol in which a diazirine functionality replaces the 5,6-double bond in the steroid nucleus, was used recently to identify cholesterol-binding proteins in neuroendocrine cells [Thiele, C., Hannah, M.J., Farenholz, F. and Huttner, W.B. (2000) Nat. Cell Biol. 2...

journal_title：FEBS letters

authors： Mintzer EA,Waarts BL,Wilschut J,Bittman R

更新日期：2002-01-16 00:00:00

abstract：:Peroxynitrite (ONOO-) has recently been implicated in connective tissue destruction in vivo. We have studied the effect of ONOO- on the activity of tissue inhibitor of metalloproteinase-1 (TIMP-1) in vitro. The inactivation of TIMP-1 by ONOO- was dose dependent with 50 microM ONOO- reducing the inhibitory activity of ...

journal_title：FEBS letters

authors： Frears ER,Zhang Z,Blake DR,O'Connell JP,Winyard PG

更新日期：1996-02-26 00:00:00

abstract：:The regulation of genes for acetylcholine receptor (AChR), myogenic factors and other muscle-specific proteins has been analyzed in experimental autoimmune myasthenia gravis (EAMG) and following denervation. The levels of the transcripts for the myogenic factors, MyoD1, myogenin and MRF4, were measured using Northern ...

journal_title：FEBS letters

authors： Asher O,Fuchs S,Zuk D,Rapaport D,Buonanno A

更新日期：1992-03-24 00:00:00

abstract：:The structure of the dimeric C-terminal domain of the HIV-1 capsid protein (CA), recently determined by X-ray crystallography (Gamble et al. (1997)), has a notable resemblance to the structure of the hepatitis B virus (HBV) capsid protein (Cp) dimer, previously determined by cryo-electron microscopy (Conway et al. (19...

journal_title：FEBS letters

authors： Zlotnick A,Stahl SJ,Wingfield PT,Conway JF,Cheng N,Steven AC

更新日期：1998-07-24 00:00:00

abstract：:The fungal metabolite brefeldin A (BFA) is known to disrupt the Golgi apparatus resulting in redistribution of Golgi proteins to the endoplasmic reticulum and inhibition of protein secretion. BFA was found to inhibit protein synthesis in rat glioma C6 cells by up to 70% between 0.1 and 1 microgram/ml. Inhibition was b...

journal_title：FEBS letters

authors： Fishman PH,Curran PK

更新日期：1992-12-21 00:00:00

abstract：:Human complement component C8gamma is an unusual complement factor since it shows no homology to other complement proteins but is a member of the lipocalin superfamily. So far, it has been found exclusively in plasma, covalently linked to C8alpha by disulfide bridging. We have used dot blot and Northern blot analyses ...

journal_title：FEBS letters

authors： Trojer P,Wojnar P,Merschak P,Redl B

更新日期：1999-03-12 00:00:00

abstract：:To identify genes whose expression is modulated by heregulin-beta1 (HRG), a regulatory polypeptide for mammary epithelial cells, we performed differential display screening of MCF-7 cell mRNA. One cDNA clone upregulated by HRG was identical to human ADP-ribosylation factor 3 (ARF3), a guanine nucleotide-binding protei...

journal_title：FEBS letters

authors： Li F,Mandal M,Mishra SK,Barnes CJ,Kumar R

更新日期：2002-07-31 00:00:00

abstract：:Mesenchymal stem cell (MSC) transplantation is used for treatment of many diseases. The paracrine role of MSCs in tissue regeneration is attracting particular attention. We investigate the role of MSC exosomes in skeletal muscle regeneration. MSC exosomes promote myogenesis and angiogenesis in vitro, and muscle regene...

journal_title：FEBS letters

authors： Nakamura Y,Miyaki S,Ishitobi H,Matsuyama S,Nakasa T,Kamei N,Akimoto T,Higashi Y,Ochi M

更新日期：2015-05-08 00:00:00