Abstract:
:Inositol 1,4,5-trisphosphate (1,4,5-InsP3) was perfused into rat dorsal root ganglion (DRG) neurons by whole-cell patch-clamp electrodes, while measuring the membrane potential. This operation evoked a transient (2-3 min) membrane hyperpolarization of about -15 mV (from -42 mV) followed by a depolarization. The membrane hyperpolarization was abolished when 30 mM EGTA was perfused together with 1,4,5-InsP3 or when 0.2 mM quinine was added to the bath solution. The hyperpolarizing response was enhanced when a low-Ca2+ EGTA-free intracellular solution was used. Two InsP2 isomers induced a different response. Our results suggest that the hyperpolarization is due to 1,4,5-InsP3-induced Ca2+ release which may trigger Ca-sensitive K+ channels to open. Present results show that cultured DRG neurons are able to respond to 1,4,5-InsP3 perfusion in the whole-cell configuration.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Wang Z,Ypey DL,Van Duijn Bdoi
10.1016/0014-5793(92)80602-dkeywords:
subject
Has Abstractpub_date
1992-06-15 00:00:00pages
124-8issue
2-3eissn
0014-5793issn
1873-3468pii
0014-5793(92)80602-Djournal_volume
304pub_type
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