Abstract:
:The white-rot fungus Phanerochaete chrysosporium has two intracellular beta-glucosidases (BGL1A and BGL1B) belonging to glycoside hydrolase (GH) family 1. BGL1B effectively hydrolyzes cellobiose and cellobionolactone, but BGL1A does not. We have determined the crystal structure of BGL1A in substrate-free and gluconolactone complexed forms. The overall structure and the characteristic of subsite -1 (glycone site) were similar to those of other known GH1 enzymes. The loop regions covering on the (beta/alpha)(8) barrel was significantly deviated, and they form a unique subsite +1 (aglycone site) of BGL1A.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Nijikken Y,Tsukada T,Igarashi K,Samejima M,Wakagi T,Shoun H,Fushinobu Sdoi
10.1016/j.febslet.2007.03.009subject
Has Abstractpub_date
2007-04-03 00:00:00pages
1514-20issue
7eissn
0014-5793issn
1873-3468pii
S0014-5793(07)00259-1journal_volume
581pub_type
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