Abstract:
:Messenger RNA from intact rat pancreatic islets, or from transformed hamster beta (HIT) cells, hybridized with the cDNA probe for type I (but not type II) phospholipase A2. The levels of phospholipase A2 mRNA increased in islets from fasted rats; they decreased in islets cultured in a high glucose concentration (control values at 5.5 mM glucose = 150 +/- 6% of those at 22 mM) which impaired subsequent insulin secretion (reduction in second-phase release = 70 +/- 11%). These studies uniquely demonstrate that type I phospholipase A2 is expressed specifically in beta cells and that nutrient availability modulates transcript levels, an effect which could that nutrient availability modulates transcript levels, an effect which could contribute to the detrimental influence of prolonged hyperglycemia on islet function.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Metz S,Holmes D,Robertson RP,Leitner W,Draznin Bdoi
10.1016/0014-5793(91)81397-qsubject
Has Abstractpub_date
1991-12-16 00:00:00pages
110-2issue
1-3eissn
0014-5793issn
1873-3468pii
0014-5793(91)81397-Qjournal_volume
295pub_type
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