Cyclic ADP-ribose-induced Ca2+ release from rat brain microsomes.

abstract：:KOR-3, also known as ORL-1, is a member of the opioid receptor family, encoding the murine receptor for orphanin FQ/nociceptin. In the current studies we have identified five different splice variants of KOR-3 in mouse brain, three of which have not been previously reported. In addition to variants with a 15 bp deleti...

journal_title：FEBS letters

authors： Pan YX,Xu J,Wan BL,Zuckerman A,Pasternak GW

更新日期：1998-09-11 00:00:00

abstract：:The interactions between clavulanate and three class C enzymes have been studied in detail. In all cases, the reactions followed branched pathways where 25-150 turnovers occurred before inactivation was completed. Reactivation rates were quite low. The poor efficiency of clavulanate as a class C inactivator appeared t...

journal_title：FEBS letters

authors： Monnaie D,Frere JM

更新日期：1993-11-22 00:00:00

abstract：:Using conserved fingerprints in the glycosyltransferase (GTase) domain of high-molecular-weight penicillin-binding proteins (PBP), a gene (mgt) encoding a putative monofunctional glycosyltransferase has been identified in Haemophilus influenzae and in other bacteria] species. Here we report the cloning of the homologo...

journal_title：FEBS letters

authors： Di Berardino M,Dijkstra A,Stüber D,Keck W,Gubler M

更新日期：1996-08-26 00:00:00

abstract：:The synthetic cRNA encoding for the major T lymphocyte K+ channel (Kv1.3) was injected into Xenopus fertilized eggs. Somites from embryos of stage 20-22 (about 40 h post-fertilization at 19 degrees C) were dissociated and myotomal muscle cells were cultured in vitro for 2 days. The whole cell configuration of the tigh...

journal_title：FEBS letters

authors： Honoré E,Guillemare E,Lesage F,Barhanin J,Lazdunski M

更新日期：1994-07-18 00:00:00

abstract：:Cellular ageing is characterized by the accumulation of oxidatively modified proteins which may be due to increased protein damage and/or decreased elimination of oxidized protein. Since the proteasome is in charge of protein turnover and removal of oxidized protein, its fate during ageing and upon oxidative stress ha...

journal_title：FEBS letters

authors： Friguet B

更新日期：2006-05-22 00:00:00

abstract：:The effects of nine drugs on the CD spectra of a synthetic calcium binding analog of site III of rabbit skeletal troponin C, can generally be divided into 3 groups: (1) that consisting of haloperidol, benperidol, molindone and promethazine, had no effect on the CD spectrum or calcium sensitivity of the apopeptide; (2)...

journal_title：FEBS letters

authors： Reid RE,Gariépy J,Hodges RS

更新日期：1983-04-05 00:00:00

abstract：:Increased expression of low voltage-activated, T-type Ca(2+) channels has been correlated with a variety of cellular events including cell proliferation and cell cycle kinetics. The recent cloning of three genes encoding T-type alpha(1) subunits, alpha(1G), alpha(1H) and alpha(1I), now allows direct assessment of thei...

journal_title：FEBS letters

authors： Chemin J,Monteil A,Briquaire C,Richard S,Perez-Reyes E,Nargeot J,Lory P

更新日期：2000-07-28 00:00:00

abstract：:The effect of lysophosphatidic acid (LPA) on the preimplantation development of mouse embryos was examined. The blastocyst rates from 2-cell and 4-cell stage embryos were significantly higher in the presence of LPA at the pronuclear stage. However, the blastocyst rate from 4-cell stage embryos was significantly lower ...

journal_title：FEBS letters

authors： Kobayashi T,Yamano S,Murayama S,Ishikawa H,Tokumura A,Aono T

更新日期：1994-08-29 00:00:00

abstract：:Vacuolar ATPases (V1V0 -ATPases) function in proton translocation across lipid membranes of subcellular compartments. We have used antibody labeling and electron microscopy to define the position of subunit C in the vacuolar ATPase from yeast. The data show that subunit C is binding at the interface of the ATPase and ...

journal_title：FEBS letters

authors： Zhang Z,Inoue T,Forgac M,Wilkens S

更新日期：2006-04-03 00:00:00

abstract：:Plastoquinone-9 (PQ-9)-depleted PSII reaction center core complex, consisting of CP47/D1/D2/Cytb-559/I, was isolated from spinach PSII particles. PQ-9, lipids and several proteins were extracted from the original PSII particles and separated by several steps of chromatography to be reconstituted into the isolated comp...

journal_title：FEBS letters

authors： Kitamura K,Ozawa S,Shiina T,Toyoshima Y

更新日期：1994-10-31 00:00:00

abstract：:Two clones were isolated by screening a shrimp hepatopancreas cDNA library with a DNA fragment obtained by PCR amplification using two oligonucleotides based on the partial protein sequence of Penaeus vanameii chymotrypsin purified earlier. One of these clones, PVC 7 contains a complete cDNA coding for a serine protea...

journal_title：FEBS letters

authors： Sellos D,Van Wormhoudt A

更新日期：1992-09-14 00:00:00

abstract：:The N-terminus of MDM2 proto-oncoprotein interacts with p53 and down modulates p53 activity by inhibiting transcriptional activity and promoting p53 degradation. MDMX is structurally related to MDM2 and also binds to p53. However, the function of MDMX has not been clarified yet. We found that MDM2 hetero-oligomerized ...

journal_title：FEBS letters

authors： Tanimura S,Ohtsuka S,Mitsui K,Shirouzu K,Yoshimura A,Ohtsubo M

更新日期：1999-03-19 00:00:00

abstract：:Using the method of contextual analysis, we have studied the collection of somatic mutations in immunoglobulin genes. It has been found that the emergence of somatic mutations can be based on the reparation of complementarity violations in the heteroduplexes corresponding to complementary palindromes or direct repeats...

journal_title：FEBS letters

authors： Kolchanov NA,Solovyov VV,Rogozin IB

更新日期：1987-04-06 00:00:00

abstract：:The present study describes a significant amino acid sequence homology between neuronal Cdk5 activator (nck5a) and an open reading frame of an unknown gene on the yeast S. cerevisiae chromosome III. A cDNA encoding a 25 kDa fragment of this yeast protein, the region containing homologous sequence to nck5a was cloned a...

journal_title：FEBS letters

authors： Huang QQ,Lee KY,Wang JH

更新日期：1996-01-02 00:00:00

abstract：:In Xenopus prophase-blocked oocytes, it is assumed that progesterone interacts with the plasma membrane to initiate a signalling cascade that ultimately leads to MPF activation. Progesterone regulates negatively the cAMP pathway through an inhibition of adenylate cyclase. However, the mechanisms linking the initial ac...

journal_title：FEBS letters

authors： De Smedt V,Rime H,Jessus C,Ozon R

更新日期：1995-11-20 00:00:00

abstract：:The integral membrane protein lactose permease of Escherichia coli was purified to homogeneity in detergent micelles. No other proteins could be detected in the final product. The molar ratio of lactose permease to lipid was less than 0.2 in detergent solution, thus the preparation was essentially lipid-free. All mole...

journal_title：FEBS letters

authors： Dornmair K

更新日期：1988-08-01 00:00:00

abstract：:Both soluble and microsomal fractions of Fusarium oxysporum contain cytochrome P-450(P-450). We report here that the P-450 in the soluble fraction was induced only when nitrate or nitrite was added to the growth medium, whereas the microsomal P-450 was synthesized regardless of the medium compositions. The reduced-CO ...

journal_title：FEBS letters

authors： Shoun H,Suyama W,Yasui T

更新日期：1989-02-13 00:00:00

abstract：:The fat mouse strain exhibits a late-onset obesity syndrome associated with a mutation in the gene encoding carboxypeptidase E (CPE). Since CPE plays a central role in the biosynthesis of a number of regulatory peptides, including gastrin, we examined the biogenesis and processing of progastrin in fat/fat mice by meas...

journal_title：FEBS letters

authors： Lacourse KA,Friis-Hansen L,Rehfeld JF,Samuelson LC

更新日期：1997-10-13 00:00:00

abstract：:The ATP synthase capacity of rat heart myocytes can be measured in sonicates of cultured cardiomyocytes. In these cells, transitions in ATP synthase capacity occur on changing to the anoxic or uncoupled state (drop in ATP synthase capacity of over 40%) or on electrically stimulating the cells to contract (rise of 70%)...

journal_title：FEBS letters

authors： Das AM,Harris DA

更新日期：1989-10-09 00:00:00

abstract：:As a first step to investigate the functional activity of gypsy virus-like particles (VLPs) we explored the possibility of preservation of its VLP in extracellular form. The preparations containing extracellular gypsy VLP from Drosophila melanogaster and D. virilis were obtained. Full-length polyA+ RNA and polyA+ RNA-...

journal_title：FEBS letters

authors： Syomin BV,Kandror KV,Semakin AB,Tsuprun VL,Stepanov AS

更新日期：1993-06-01 00:00:00

abstract：:Isolation and characterization of the cDNA coding for the 216-residue Xenopus laevis prion protein is reported. Existence of this protein in amphibians was suggested by an EST fragment (accession number BG813008), while a conclusive demonstration is presented here. This protein exhibits a higher identity level to avia...

journal_title：FEBS letters

authors： Strumbo B,Ronchi S,Bolis LC,Simonic T

更新日期：2001-11-16 00:00:00

abstract：:The primary structure of nuclear factor-I (CTF/NF-I), a eukaryotic regulatory DNA-binding protein involved in both DNA replication and gene transcription, and the secondary structure predictable from it, are compared here with those of a number of prokaryotic acetylases. Hydropathy and Chou-Fasman analyses reveal that...

journal_title：FEBS letters

authors： Oikarinen J,Mannermaa RM

更新日期：1990-10-29 00:00:00

abstract：:Two highly contrasted images depict genomes: at first sight, genes appear to be distributed randomly along the chromosome. In contrast, their organisation into operons (or pathogenicity islands) suggests that, at least locally, related functions are in physical proximity. Analysis of the codon usage bias in orthologou...

journal_title：FEBS letters

authors： Rocha EP,Sekowska A,Danchin A

更新日期：2000-06-30 00:00:00

abstract：:An experimental procedure is described for the reproducible production of hybridoma cells using the electrofusion technique. High yields can be obtained when fusion is performed in isotonic inositol solutions containing Ca2+ and Mg2+ in a ratio of 1:5 in the millimolar range. The hybridoma cells are transferred 10 min...

journal_title：FEBS letters

authors： Vienken J,Zimmermann U

更新日期：1985-03-25 00:00:00

abstract：:The protozoan parasite and human pathogen Entamoeba histolytica is protected against killing by its own lytic effector proteins. Amoebae withstand doses of amoebapores, their pore-forming polypeptides, that readily kill human Jurkat T cells. Moreover, the polypeptides do not bind to the amoebic surface membrane as evi...

journal_title：FEBS letters

authors： Andrä J,Berninghausen O,Leippe M

更新日期：2004-04-23 00:00:00

abstract：:Lipoprotein MtsA is a critical component of MtsABC responsible for iron binding and transport in the Gram-positive bacterium Streptococcus pyogenes. The present collective experimental data establish that Fe(2+) is the primary binding ion for MtsA under optimal physiologically relevant conditions. The binding affiniti...

journal_title：FEBS letters

authors： Sun X,Ge R,Chiu JF,Sun H,He QY

更新日期：2008-04-16 00:00:00

abstract：:The dynamics of changes in membrane potential, cytosolic free Ca2+, [Ca2+]i and immunoreactive insulin release were assessed in RINm5F cells. Membrane depolarization and a rise in [Ca2+]i preceded the stimulation of insulin release by D-glyceraldehyde. Forskolin, which raised the cellular cyclic AMP levels, stimulated...

journal_title：FEBS letters

authors： Wollheim CB,Ullrich S,Pozzan T

更新日期：1984-11-05 00:00:00

abstract：:This paper reports the genomic analysis of the strain CBS7064 of Pichia sorbitophila, a homothallic diploid yeast. We sequenced 4829 random sequence tags of about 1 kb and compared them to the Saccharomyces cerevisiae gene products. Approximately 1300 nuclear genes, 22 tRNAs, the rDNA locus, and six mitochondrial gene...

journal_title：FEBS letters

authors： de Montigny J,Spehner C,Souciet J,Tekaia F,Dujon B,Wincker P,Artiguenave F,Potier S

更新日期：2000-12-22 00:00:00

abstract：:Functional analysis of heterologously expressed TaTIP2;2 by means of stopped-flow spectrometric studies provide evidence for water and ammonia conductivity. A series of experiments under increasing pH indicate that the gaseous NH(3), rather than the ammonium ion NH(4)(+) was transported. Results from inhibitor studies...

journal_title：FEBS letters

authors： Bertl A,Kaldenhoff R

更新日期：2007-11-27 00:00:00

abstract：:We show that some arbitrarily chosen DNA sequences have the ability to influence the positioning of RecA monomers in RecA-DNA complexes. The preferential phase of binding of RecA monomers is shown to depend on the DNA sequence and its nucleotide composition. A simple rearrangement of bases in a limited DNA stretch inf...

journal_title：FEBS letters

authors： Volodin AA,Smirnova EA,Bocharova TN,Camerini-Otero RD

更新日期：2003-07-10 00:00:00