Abstract:
:Thrombin is a multifunctional enzyme involved in coagulation, cell modulation and inflammation. We recently reported a novel membrane-associated prothrombin activator, abbreviated as MAPA, found in cultured fibroblasts and glial cell lines. In this study, we examined the physiological role of this enzyme. MAPA-like activity was detected in the liver, kidney, lung and heart but not in the spleen or brain in normal mice. To examine whether MAPA participates in biological reactions, hepatic and renal injury were induced by administration of CCl4 and HgCl2, respectively. MAPA-like activity was specifically increased in the injured tissues: the activity was elevated by about 100-fold in 48 h in the liver and increased by about 5-fold in 12 h in the kidney. Their enzymatic properties were the same as those of MAPA in 8C feline kidney fibroblast cells. Phospholipids are required for activation of prothrombin by MAPA obtained from both 8C cells and tissues. These results suggest that MAPA activates prothrombin on the cell surface in injured tissue and participates in inflammation and regeneration associated with tissue injury.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Shikamoto Y,Shibusawa S,Okuyama I,Morita Tdoi
10.1016/s0014-5793(97)00852-1subject
Has Abstractpub_date
1997-08-04 00:00:00pages
526-30issue
3eissn
0014-5793issn
1873-3468pii
S0014-5793(97)00852-1journal_volume
412pub_type
杂志文章相关文献
FEBS LETTERS文献大全abstract::Bovine heart cytochrome-c oxidase was reconstituted in liposomes and the kinetics of cytochrome c oxidation were measured by the polarographic and photometric method under uncoupled conditions in the presence of various polyvalent anions. In order to distinguish between specific and unspecific ionic effects of ATP, th...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(86)80018-7
更新日期:1986-10-20 00:00:00
abstract::Poly(A)+ RNA isolated from post-mortem human hypothalami has been used to characterize the poly-protein precursors to vasopressin and oxytocin. Translation in a cell-free system and subsequent immuno-precipitation with antibodies raised against either vasopressin or neurophysin identified a product of Mr 19000 (prepro...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(85)80069-7
更新日期:1985-11-25 00:00:00
abstract::Cytochrome bd is one of the two terminal ubiquinol oxidases in the respiratory chain of Escherichia coli catalyzing reduction of O2 to H2O. The enzyme is expressed under low oxygen tension; due to high affinity for O2 it is isolated mainly as a stable oxygenated complex. Direct measurement of O2 binding to heme d in t...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2005.07.011
更新日期:2005-08-29 00:00:00
abstract::Wilms tumor (WT) is a genetically heterogeneous childhood kidney tumor. Several genetic mutations have been identified in WT patients, including inactivation of WTX, somatic stabilizing CTNNB1, and p53 mutations. However, the molecular mechanisms in tumorigenesis remain largely unexplored. Stat3 is a transcription fac...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2013.01.012
更新日期:2013-03-18 00:00:00
abstract::An up to 14-fold increase in total hexokinase activity induced by low-frequency stimulation in rat fast-twitch muscle was followed by a rapid decay in enzyme activity after cessation of stimulation. In vivo labeling revealed that these alterations were related to rapid changes in [35S]methionine incorporation into hex...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(90)80574-3
更新日期:1990-02-26 00:00:00
abstract::Immunometabolism focuses on the mechanisms regulating the impact of metabolism on lymphocyte activity and autoimmunity outbreak. The adipose tissue is long known to release adipokines, either pro- or anti-inflammatory factors bridging nutrition and immune function. More recently, adipocytes were discovered to also rel...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1002/1873-3468.12733
更新日期:2017-10-01 00:00:00
abstract::Zinc metallopeptidases that contain the His-Glu-Xaa-Xaa-His (HEXXH) motif generally have a third ligand of the metal ion that may be either a Glu residue (in clan MA) or a His residue (in clan MB) (Rawlings and Barrett (1995) Methods Enzymol. 248, 183-228). Thimet oligopeptidase has not yet been assigned to either cla...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)01032-1
更新日期:1998-09-11 00:00:00
abstract::Primary cultures of rat dorsal root ganglionic (DRG) cells were stained with isoform-specific antibodies against non-muscle myosin II. Antibodies against the brain type myosin (MIIB) stained the peripheries of growth cones and non-neuronal cells. Double staining of the cells with the anti-myosin antibodies and rhodami...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(92)81374-u
更新日期:1992-10-19 00:00:00
abstract::Streptococci are a broad group of Gram-positive bacteria. This genus includes various human pathogens causing significant morbidity and mortality. Two of the most important human pathogens are Streptococcus pneumoniae (pneumococcus) and Streptococcus pyogenes (group A streptococcus or GAS). Streptococcal pathogens hav...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1002/1873-3468.13872
更新日期:2020-08-01 00:00:00
abstract::Resonance Raman spectroscopy was applied to monitor the effects of chloroquine (CQ) treatment on cultures of Plasmodium falciparum trophozoites. A number of bands assigned to A(1g) and B(1g) modes characteristic of the haemozoin aggregate are reduced in intensity in the CQ-treated cells, however, no bands from the CQ ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2008.02.062
更新日期:2008-04-02 00:00:00
abstract::Acetylcholine (1-10 microM) depolarized the membrane and stimulated glucose-induced bursts of electrical activity in mouse pancreatic B-cells. The acetylcholine effects were mimicked by muscarine while nicotine had no effect on membrane potential. Pirenzepine, an antagonist of the classical M1-type muscarinic receptor...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(89)80669-6
更新日期:1989-06-05 00:00:00
abstract::ROPs constitute a family of plant-specific, RHO-like small GTPases that serve as molecular switches in a wide range of signaling pathways. The activities of ROPs are regulated by guanine nucleotide exchange factors (GEFs). ROP11, a member of the ROP GTPase family in Arabidopsis, is a negative regulator of multiple ABA...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2012.03.040
更新日期:2012-05-07 00:00:00
abstract::Niemann-Pick C (NPC) disease is a progressive neurological disorder in which cholesterol, gangliosides and bis-monoacylglycerol phosphate accumulate in late endosomes/lysosomes. This disease is caused by mutations in either the NPC1 or NPC2 gene. NPC1 and NPC2 are involved in egress of lipids, particularly cholesterol...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2006.06.008
更新日期:2006-10-09 00:00:00
abstract::The cleavable prepiece of the precursor to yeast cytochrome c oxidase subunit IV (an imported mitochondrial protein) was attached to the amino-terminus of mouse dihydrofolate reductase (a cytosolic protein) by gene fusion. The resulting fusion protein was imported into the matrix of isolated, energized yeast mitochond...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(84)80622-5
更新日期:1984-12-10 00:00:00
abstract::Proteins and peptides are readily and specifically modified at their sulfhydryl groups by the vapors of a mixture of 4-vinylpyridine and tributylphosphine. The phenylthiohydantoin derivative of S-beta-(4-pyridylethyl)cysteine formed during sequence analysis is easily detectable in current identification systems. ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(87)81558-2
更新日期:1987-02-09 00:00:00
abstract::Two RNA silencing-related phenomena, quelling and meiotic silencing by unpaired DNA (MSUD) have been identified in the fungus Neurospora crassa. Similar to the case with the siRNA and miRNA pathways in Drosophila, different sets of protein components including RNA-dependent RNA polymerase, argonaute and dicer, are use...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2005.08.016
更新日期:2005-10-31 00:00:00
abstract::The essential role of Factor VIII:C (FVIII:C, anti-hemophilia factor A) as a cofactor for Factor IXa-dependent activation of Factor X has been established. In this paper, we describe that capillary endothelial cells from bovine adrenal medulla express active FVIII:C gene. Accumulation of FVIII:C in conditioned media f...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(92)80831-z
更新日期:1992-07-13 00:00:00
abstract::Three DNA restriction fragments designated tyrT, 102-mer and 70-mer, have been used as substrates for footprinting studies using DNase I in the presence of the structurally similar antibiotics chartreusin and elsamicin A. The sequence-selective binding sites of the antibiotics can be mapped in regions which are rich i...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(91)80872-z
更新日期:1991-11-04 00:00:00
abstract::Activation-induced cytidine deaminase (AID), a member of the APOBEC family that induces antibody diversification, has been shown to inhibit the replication of hepatitis B virus, Kaposi's sarcoma-associated herpesvirus, and retro-transposons. However, whether AID can inhibit human immunodeficiency virus 1 (HIV-1) repli...
journal_title:FEBS letters
pub_type: 信件
doi:10.1002/1873-3468.12954
更新日期:2018-01-01 00:00:00
abstract::Sequence comparisons among methionyl-tRNA synthetases from different organisms reveal only one block of homology beyond the last beta strand of the mononucleotide fold. We have introduced a series of semi-conservative amino acid replacements in the conserved motif of yeast methionyl-tRNA synthetase. The results indica...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(91)81073-h
更新日期:1991-09-09 00:00:00
abstract::Synaptotagmin I, the calcium sensor for neurotransmission, is palmitoylated. We have identified the palmitoylation sites as five cysteine residues located between the transmembrane and cytoplasmic regions. In contrast to wild-type synaptotagmin, the non-acylated mutant is not converted to the endoglycosidase-H-resista...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(03)00449-6
更新日期:2003-06-05 00:00:00
abstract::Potential antioxidant properties of therapeutically achievable concentrations of the protonated, active form of omeprazole (OM) were investigated in vitro at specific acidic pH values to mimic intragastric conditions in the clinical setting. We found that OM is a powerful scavenger of hypochlorous acid (HOCl) even at ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(96)00155-x
更新日期:1996-03-11 00:00:00
abstract::We investigated transactivation by NANOG in regulating growth and differentiation factor 3 (GDF3) expression in NCCIT cells. GDF3 expression was affected by shRNA-mediated downregulation and by exogenous overexpression of NANOG specifically, as well as by retinoic acid-mediated differentiation. GDF3 transcription was ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2012.08.013
更新日期:2012-09-21 00:00:00
abstract::Lactase phlorizin hydrolase (LPH; EC 3.2.1.62) is a membrane-bound, family 1 beta-glycosidase found on the brush border of the mammalian small intestine. LPH, purified from sheep small intestine, was capable of hydrolysing a range of flavonol and isoflavone glycosides. The catalytic efficiency (k(cat)/K(m)) for the hy...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(00)01211-4
更新日期:2000-02-25 00:00:00
abstract::Macrolides are a group of antibiotics structurally characterized by a macrocyclic lactone to which one or several deoxy-sugar moieties are attached. The sugar moieties are transferred to the different aglycones by glycosyltransferases (GTF). The OleI GTF of an oleandomycin producer, Streptomyces antibioticus, catalyze...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(00)01721-x
更新日期:2000-07-07 00:00:00
abstract::Chloride intracellular channel proteins (CLICs) are distinct from most ion channels in that they have both soluble and integral membrane forms. CLICs are highly conserved in chordates, with six vertebrate paralogues. CLIC-like proteins are found in other metazoans. CLICs form channels in artificial bilayers in a proce...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2010.01.027
更新日期:2010-05-17 00:00:00
abstract::Antibodies were made to a thirteen amino acid synthetic peptide corresponding to the C-terminal portion of the glutamate (glu) receptor, GluR-A. The immunoprecipitation of kainic acid (KA) and alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) binding sites by the anti-peptide antibodies was studied using...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(90)80529-r
更新日期:1990-12-10 00:00:00
abstract::A mathematical model was developed to estimate flux control coefficients (Co) from titration studies with specific non-competitive inhibitors. In contrast to the normally used graphical determination the model pays regard to the dissociation equilibrium (KD) that exists between inhibitor and its binding sites (Eo) as ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(90)81355-r
更新日期:1990-11-12 00:00:00
abstract::Cellular homeostasis in response to glucose availability is maintained through the tight coordination of various physiological processes, including cell proliferation, transcription, and metabolism. In this study, we use the budding yeast Saccharomyces cerevisiae to identify proteins implicated in carbon source-depend...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1002/1873-3468.13722
更新日期:2020-04-01 00:00:00
abstract::Using a cDNA library from bovine adrenal medulla, and, subsequently, a bovine genomic library, we have isolated the gene coding for a non inactivating potassium channel. This gene encodes a 597-amino acid protein which we have called BAK5 as its sequence is very similar to members of Kv1.5 potassium channel family. Ne...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)01117-6
更新日期:1994-11-07 00:00:00