Abstract:
:Using a cDNA library from bovine adrenal medulla, and, subsequently, a bovine genomic library, we have isolated the gene coding for a non inactivating potassium channel. This gene encodes a 597-amino acid protein which we have called BAK5 as its sequence is very similar to members of Kv1.5 potassium channel family. Neuroblastoma cells (Neuro-2a cell line) were stably transfected with BAK5 DNA. Protein expression was under the control of a heat-shock promoter. Transfected cells showed a current highly selective for potassium, insensitive to tetraethylammonium but reversibly blocked by 4-aminopyridine. Oocytes injected with BAK5 mRNA also expressed a potassium current with the same characteristics.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
García-Guzmán M,Sala F,Criado M,Sala Sdoi
10.1016/0014-5793(94)01117-6subject
Has Abstractpub_date
1994-11-07 00:00:00pages
173-6issue
2eissn
0014-5793issn
1873-3468pii
0014-5793(94)01117-6journal_volume
354pub_type
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