Abstract:
:A functional NAD molecule was immobilized at the active site of Alcohol dehydrogenase within a proteic membrane. The presence and the functionality of the cofactor was checked by fluorescence analysis. The dehydrogenase NAD membrane does not require addition of soluble cofactor for its activity. The system represents a new worthwhile approach because both problems of retention and regeneration of cofactor are solved. The method can be used not only for industrial and analytical applications but also to try to get a better understanding of the kinetics and mechanisms of the catalytic action of dehydrogenase.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Legoy MD,le Moullec JM,Thomas Ddoi
10.1016/0014-5793(78)80970-3subject
Has Abstractpub_date
1978-10-15 00:00:00pages
335-8issue
2eissn
0014-5793issn
1873-3468journal_volume
94pub_type
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