Domain interactions stabilize the alternatively folded state of an antibody Fab fragment.

abstract：:A large number of protein-protein interactions involved turn or loop regions. The excised linear peptides from these regions reveal complex conformational averaging. To circumvent this motional averaging and to stabilize the beta-turn conformation, extensive effort has been devoted to the design of constrained peptido...

journal_title：FEBS letters

authors： Lee MS,Gardner B,Kahn M,Nakanishi H

更新日期：1995-02-13 00:00:00

abstract：:Lactase phlorizin hydrolase (LPH; EC 3.2.1.62) is a membrane-bound, family 1 beta-glycosidase found on the brush border of the mammalian small intestine. LPH, purified from sheep small intestine, was capable of hydrolysing a range of flavonol and isoflavone glycosides. The catalytic efficiency (k(cat)/K(m)) for the hy...

journal_title：FEBS letters

authors： Day AJ,Cañada FJ,Díaz JC,Kroon PA,Mclauchlan R,Faulds CB,Plumb GW,Morgan MR,Williamson G

更新日期：2000-02-25 00:00:00

abstract：:Zinc metallopeptidases that contain the His-Glu-Xaa-Xaa-His (HEXXH) motif generally have a third ligand of the metal ion that may be either a Glu residue (in clan MA) or a His residue (in clan MB) (Rawlings and Barrett (1995) Methods Enzymol. 248, 183-228). Thimet oligopeptidase has not yet been assigned to either cla...

journal_title：FEBS letters

authors： Chen JM,Stevens RA,Wray PW,Rawlings ND,Barrett AJ

更新日期：1998-09-11 00:00:00

abstract：:Islet amyloid polypeptide (IAPP) is the 37-amino acid peptide subunit of amyloid found in pancreatic islets of type 2 diabetic patients and in insulinomas. Recently, we isolated the human gene encoding IAPP [(1988) FEBS Lett. 239, 227-232]. We now report the nucleotide sequences of a human insulinoma cDNA encoding a c...

journal_title：FEBS letters

authors： Mosselman S,Höppener JW,Lips CJ,Jansz HS

更新日期：1989-04-10 00:00:00

abstract：:Perhydrohistrionicotoxin at micromolar concentrations blocked the nicotine-evoked transmitter release from perfused striatal (dopaminergic) and hippocampal (cholinergic) nerve terminals. Perhydrohistrionicotoxin failed to compete with [3H]nicotine for its high-affinity binding site in rat brain, suggesting that the ac...

journal_title：FEBS letters

authors： Rapier C,Wonnacott S,Lunt GG,Albuquerque EX

更新日期：1987-02-23 00:00:00

abstract：:The remarkable stability of c-myc during oogenesis contrasts with its degradation during the early developmental period in Xenopus laevis. Three evolutionary conserved motifs found in the 3'-untranslated region of Xenopus c-myc RNAs have been analyzed for a possible role in c-myc RNA degradation. No specific degradati...

journal_title：FEBS letters

authors： Tchang F,Vriz S,Méchali M

更新日期：1991-10-21 00:00:00

abstract：:The oxysterol binding proteins (OSBPs) are believed to control cholesterol homeostasis but their precise mechanism of action is not well understood. The Dictyostelium osbA gene encodes a predicted OSBP, OSBPa, which lacks the PH domain that in most other OSBPs directs targetting to the Golgi. OSBPa instead localises s...

journal_title：FEBS letters

authors： Fukuzawa M,Williams JG

更新日期：2002-09-11 00:00:00

abstract：:Sequence comparison based on Hydrophobic Cluster Analysis procedures shows that the extracellular approximately 200 amino acids domains of cytokines receptors belonging to the Cytokine/Growth hormone/Prolactin receptor family and to the Interferon one are organized in two homologous subdomains. Further, comparison of ...

journal_title：FEBS letters

authors： Thoreau E,Petridou B,Kelly PA,Djiane J,Mornon JP

更新日期：1991-04-22 00:00:00

abstract：:The effect of lysophosphatidic acid (LPA) on the preimplantation development of mouse embryos was examined. The blastocyst rates from 2-cell and 4-cell stage embryos were significantly higher in the presence of LPA at the pronuclear stage. However, the blastocyst rate from 4-cell stage embryos was significantly lower ...

journal_title：FEBS letters

authors： Kobayashi T,Yamano S,Murayama S,Ishikawa H,Tokumura A,Aono T

更新日期：1994-08-29 00:00:00

abstract：:We introduced rat ornithine transcarbamylase (OTC) gene into OTC-deficient spf-ash mice by mating spf-ash heterozygotes with transgenic mice which carried recombinant DNA composed of 1.3 kb of the 5' flanking region of the gene fused onto rat OTC cDNA. The liver OTC activity of hemizygous spf-ash mice which carried th...

journal_title：FEBS letters

authors： Shimada T,Noda T,Tashiro M,Murakami T,Takiguchi M,Mori M,Yamamura K,Saheki T

更新日期：1991-02-25 00:00:00

abstract：:Communication between endothelial and smooth muscle cells (SMCs) contributes to atherosclerosis induced by atherogenic factors, such as oxide LDL. Asymmetric dimethylarginine (ADMA), a newly found cardiovascular risk factor, accumulates in the culture medium of oxide LDL (oxLDL)-treated endothelial cells and positivel...

journal_title：FEBS letters

authors： Sun L,Zhang T,Yu X,Xin W,Lan X,Zhang D,Huang C,Du G

更新日期：2011-09-02 00:00:00

abstract：:Ku, a heterodimer of 70- and 80-kDa subunits, plays a general role in the metabolism of DNA ends in eukaryotic cells, including double-strand DNA break repair, V(D)J recombination, and maintenance of telomeres. We have utilized the yeast two-hybrid system to identify Ku70-interacting proteins other than Ku80. Two reac...

journal_title：FEBS letters

authors： Song K,Jung D,Jung Y,Lee SG,Lee I

更新日期：2000-09-08 00:00:00

abstract：:We have set up a model system for familial amyotrophic lateral sclerosis (FALS) by transfecting human neuroblastoma cell line SH-SY5Y with plasmids directing constitutive expression of either wild-type human Cu,Zn superoxide dismutase (Cu,ZnSOD) or a mutant of this enzyme (G93A) associated with FALS. We have tested mi...

journal_title：FEBS letters

authors： Carrì MT,Ferri A,Battistoni A,Famhy L,Gabbianelli R,Poccia F,Rotilio G

更新日期：1997-09-08 00:00:00

abstract：:The amino acid sequence of ubiquitin from Entamoeba histolytica, as deduced from a cDNA nucleotide sequence, deviated at six positions from the consensus of all other known ubiquitins (ranging from Trypanosoma cruzi to Homo sapiens). The corresponding residues were scattered over the primary sequence, but came close t...

journal_title：FEBS letters

authors： Wöstmann C,Tannich E,Bakker-Grunwald T

更新日期：1992-08-10 00:00:00

abstract：:Immature Drosophila rhodopsin is N-glycosylated, but undergoes complete deglycosylation during the process of protein maturation. In order to elucidate the site of glycosylation and its role in rhodopsin synthesis, we investigated the in vitro and in vivo synthesis of rhodopsin whose putative N-glycosylation sites (As...

journal_title：FEBS letters

authors： Katanosaka K,Tokunaga F,Kawamura S,Ozaki K

更新日期：1998-03-13 00:00:00

abstract：:Plants have evolved to cope with fluctuations in water supply by gating their water channels known as aquaporins. During flooding, a rapid drop of cytosolic pH due to anoxia leads to a simultaneous closure of the aquaporins in the plasma membrane. The closing mechanism has been suggested to involve a conserved histidi...

journal_title：FEBS letters

authors： Frick A,Järvå M,Törnroth-Horsefield S

更新日期：2013-04-02 00:00:00

abstract：:The timely and reliable processing of auditory and vestibular information within the inner ear requires highly sophisticated sensory transduction pathways. On a cellular level, these demands are met by hair cells, which respond to sound waves - or alterations in body positioning - by releasing glutamate-filled synapti...

journal_title：FEBS letters

authors： Pangrsic T,Vogl C

更新日期：2018-11-01 00:00:00

abstract：:Polypeptides rich in glutamic acid are strong inhibitors purified from isolated nuclei of Xenopus laevis oocytes of casein kinase II. The presence of tyrosine in these peptides greatly enhances their inhibitory capacity. Using casein as a substrate, copolyglu:tyr (4:1) has an I50 value of 20 nM, 250 fold lower than th...

journal_title：FEBS letters

authors： Tellez R,Gatica M,Allende CC,Allende JE

更新日期：1990-06-04 00:00:00

abstract：:We recently reported that production of reactive oxygen species (ROS) is essential for auxin-induced gravitropic signaling. Here, we investigated the role of phosphatidylinositol 3-kinase and its product, PtdIns(3)P, in auxin-mediated ROS production and the root gravitropic response. Pretreatment with LY294002, an inh...

journal_title：FEBS letters

authors： Joo JH,Yoo HJ,Hwang I,Lee JS,Nam KH,Bae YS

更新日期：2005-02-14 00:00:00

abstract：:Nanoscale protein supported phospholipid bilayer discs, or Nanodiscs, were produced for the purpose of studying the phase transition behavior of the incorporated lipids. Nanodiscs and vesicles were prepared with two phospholipids, dipalmitoyl phosphatidylcholine and dimyristoyl phosphatidylcholine, and the phase trans...

journal_title：FEBS letters

authors： Shaw AW,McLean MA,Sligar SG

更新日期：2004-01-02 00:00:00

abstract：:The effect of oxLDL on CD36 expression has been assessed in preadipocytes induced to differentiate. Novel evidence is provided that oxLDL induce a peroxisome proliferator-activated receptor gamma-independent CD36 overexpression, by up-regulating nuclear factor erythroid 2 (NF-E2)-related factor 2 (Nrf2). The nuclear t...

journal_title：FEBS letters

authors： D'Archivio M,Scazzocchio B,Filesi C,Varì R,Maggiorella MT,Sernicola L,Santangelo C,Giovannini C,Masella R

更新日期：2008-06-25 00:00:00

abstract：:A ratio-fluorescence assay was developed for on-line localization and quantification of lipid oxidation in living cells. The assay explores the oxidative sensitivity of C11-BODIPY(581/591). Upon oxidation, the fluorescence of this fluorophore shifts from red to green. The probe incorporates readily into cellular membr...

journal_title：FEBS letters

authors： Pap EH,Drummen GP,Winter VJ,Kooij TW,Rijken P,Wirtz KW,Op den Kamp JA,Hage WJ,Post JA

更新日期：1999-06-25 00:00:00

abstract：:As a member of the dynamin superfamily human guanylate-binding protein 1 (hGBP1) binds and hydrolyses GTP thereby undergoing structural changes which lead to self-assembly of the protein. Here, we employ the reactivity of hGBP1 with a cysteine reactive compound in order to monitor structural changes imposed by GTP bin...

journal_title：FEBS letters

authors： Vöpel T,Kunzelmann S,Herrmann C

更新日期：2009-06-18 00:00:00

abstract：:The covalent binding of pyridoxal 5'-phosphate (PLP) to human serum albumin (HSA) is important in the regulation of PLP metabolism. In plasma, PLP is bound to HSA at a single high-affinity and at two or more nonspecific sites. To characterize the primary PLP binding site, HSA was incubated with [3H] PLP, and the Schif...

journal_title：FEBS letters

authors： Bohney JP,Fonda ML,Feldhoff RC

更新日期：1992-02-24 00:00:00

abstract：:Human endothelial cells exposed to H2O2 showed reduced CREP DNA binding activity, enhanced HSF activation, and no induction of NFkappaB binding activity. Interestingly, H2O2 was able to induce NFkappaB subunit p65 translocation in the nucleus. In contrast, cells exposed to TNF alpha showed enhanced CREP binding activi...

journal_title：FEBS letters

authors： Jornot L,Petersen H,Junod AF

更新日期：1997-10-27 00:00:00

abstract：:cDNAs encoding the isoform I alpha of the cGMP-dependent protein kinase were isolated from a bovine trachea smooth muscle cDNA library constructed in lambda gt10. The deduced protein sequence is identical with the protein sequence obtained by Edman degradation of the bovine lung enzyme [(1984) Biochemistry 23, 4207-42...

journal_title：FEBS letters

authors： Wernet W,Flockerzi V,Hofmann F

更新日期：1989-07-17 00:00:00

abstract：:Treatment of rat thymus cell suspensions with dexamethasone resulted in inhibition of engaged RNA polymerase A, without significant change in free pool activity. Studies with the re-initiation inhibitor, rifamycin AF/0-13, and measurements of numbers of RNA polymerase A molecules and of elongation rates showed that th...

journal_title：FEBS letters

authors： Dembinski TC

更新日期：1984-07-23 00:00:00

abstract：:Interaction of lectins with cell surface determinants may alter membrane properties. Using trypsinized rabbit erythrocytes as model we tested the capacity of an endogenous lectin in this respect. Galectin-1 is a member of an adhesion/growth-regulatory family known to interact for example with ganglioside GM(1) and als...

journal_title：FEBS letters

authors： Gupta RK,Pande AH,Gulla KC,Gabius HJ,Hajela K

更新日期：2006-03-06 00:00:00

abstract：:The white-rot fungus Phanerochaete chrysosporium has two intracellular beta-glucosidases (BGL1A and BGL1B) belonging to glycoside hydrolase (GH) family 1. BGL1B effectively hydrolyzes cellobiose and cellobionolactone, but BGL1A does not. We have determined the crystal structure of BGL1A in substrate-free and gluconola...

journal_title：FEBS letters

authors： Nijikken Y,Tsukada T,Igarashi K,Samejima M,Wakagi T,Shoun H,Fushinobu S

更新日期：2007-04-03 00:00:00

abstract：:Proteasomes were purified from human hepatoma tissues, and their sensitivities to Na+ and K+ were examined. At concentrations of 10 mM or more, these cations were found to inhibit completely polylysine-activated casein degradation by the purified proteasomes. They also strongly inhibited the hydrolyses of peptides, al...

journal_title：FEBS letters

authors： Seol JH,Park SC,Ha DB,Chung CH,Tanaka K,Ichihara A

更新日期：1989-04-24 00:00:00