The complete islet amyloid polypeptide precursor is encoded by two exons.


:Islet amyloid polypeptide (IAPP) is the 37-amino acid peptide subunit of amyloid found in pancreatic islets of type 2 diabetic patients and in insulinomas. Recently, we isolated the human gene encoding IAPP [(1988) FEBS Lett. 239, 227-232]. We now report the nucleotide sequences of a human insulinoma cDNA encoding a complete IAPP precursor, and of the corresponding parts of the IAPP gene. Two exons, which are approx. 5 kb apart in the human genome, encode the 89-amino acid pre-pro-IAPP. At least one additional exon is present further upstream in the IAPP gene. A putative signal sequence at the amino-terminus of the precursor suggests that IAPP is a secreted protein.




FEBS letters


Mosselman S,Höppener JW,Lips CJ,Jansz HS




Has Abstract


1989-04-10 00:00:00














  • Purification and characterization of ubiquitin from mammalian testis.

    abstract::Ubiquitin was extracted from testis of 4 mammals and purified to homogeneity by gel filtration chromatography. Amino acid compositions and NH2-terminal sequences were found to be identical in the 4 species and with calf thymus ubiquitin. Ubiquitin conformation was shown to be very sensitive to oxidation. Improved meth...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Loir M,Caraty A,Lanneau M,Menezo Y,Muh JP,Sautiere P

    更新日期:1984-04-24 00:00:00

  • Differential internalisation of mGluR1 splice variants in response to agonist and phorbol esters in permanently transfected BHK cells.

    abstract::The internalisation of metabotropic glutamate receptor (mGluR1alpha) and its splice variant (mGluR1beta), in response to agonist and phorbol esters (PMA), has been studied. Both mGluR1alpha and mGluR1beta exhibit a similar rate of internalisation following PMA treatment, with a shift in their distribution from plasma ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Ciruela F,McIlhinney RA

    更新日期:1997-11-24 00:00:00

  • The use of carotenoids and oxonol VI as probes for membrane potential in proteoliposomes.

    abstract::Carotenoids present in lipids extracted from the cyanobacterium Synechococcus 6716 indicate trans-membrane potential in proteoliposomes reconstituted from these lipids and the ATPase complex isolated from the same organism. A carotenoid absorbance band shift to a longer wavelength is obtained with valinomycin-induced ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: van Walraven HS,Krab K,Hagendoorn MJ,Kraayenhof R

    更新日期:1985-05-06 00:00:00

  • Metabolic pathways in Ehrlich ascites tumor cells recovering from a low bioenergetic status.

    abstract::Ehrlich ascites tumor cells were found to be in a low bioenergetic status, as evaluated by acridine orange uptake and ATP content, when resuspended in a glucose medium shortly after removal from the animal. Dye uptake as well as ATP content then increased for about 2 h at room temperature. This effect was only slightl...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Dell'Antone P

    更新日期:1994-08-22 00:00:00

  • Attenuated cytotoxicity but enhanced betafibril of a mutant amyloid beta-peptide with a methionine to cysteine substitution.

    abstract::Amyloid-beta peptide (Abeta), the major constituent of senile plaques in the Alzheimer's disease (AD) brain, is the main source of oxidative stress leading to neurodegeneration. The methionine residue in this peptide is reported to be responsible for neurotoxicity. Structurally similar substitution with methionine 35 ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Dai XL,Sun YX,Jiang ZF

    更新日期:2007-04-03 00:00:00

  • Unusual enzyme characteristics of aspartyl-tRNA synthetase from hyperthermophilic archaeon Pyrococcus sp. KOD1.

    abstract::The aspA gene, encoding the aspartyl-tRNA synthetase (AspRS) from the hyperthermophilic archaeon Pyrococcus sp. KOD1, was expressed in Escherichia coli. The KOD1 AspRS, which was purified to homogeneity and was shown to be functional in dimeric form, aminoacylated tRNA from KOD1. The optimum temperature for this activ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Fujiwara S,Lee SG,Haruki M,Kanaya S,Takagi M,Imanaka T

    更新日期:1996-09-23 00:00:00

  • Phenylalanine ammonia-lyase immobilized in semipermeable microcapsules for enzyme replacement in phenylketonuria.

    abstract::Phenylalanine ammonia-lyase immobilized within semipermeable microcapsules has an assayed enzyme activity which is 20% +/- 4% of the enzyme in free solution. The Km for the immobilized enzyme remained the same as that of the free enzyme. The pH optimum also remained unchanged at pH 8.5 +/- 1.0. At the lower pH range, ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Bourget L,Chang TM

    更新日期:1985-01-21 00:00:00

  • Skp2 induction and phosphorylation is associated with the late G1 phase of proliferating rat hepatocytes.

    abstract::The changes in phosphoproteins purified with the affinity peptide p9CKShs1 were analyzed from extracts of regenerating rat livers in order to define some G1 and G1/S regulations characteristic of mature hepatocytes stimulated to proliferate. We observed a 47 kDa phosphoprotein that occurred first at the end of G1 befo...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Bilodeau M,Talarmin H,Ilyin G,Rescan C,Glaise D,Cariou S,Loyer P,Guguen-Guillouzo C,Baffet G

    更新日期:1999-06-11 00:00:00

  • P1,P3-bis(5'-adenosyl)triphosphate (Ap3A) as a substrate and a product of mammalian tryptophanyl-tRNA synthetase.

    abstract::Bovine tryptophanyl-tRNA synthetase (TrpRS, E.C. is unable to catalyze in vitro formation of Ap4A in contrast to some other aminoacyl-tRNA synthetases. However, in the presence of L-tryptophan, ATP-Mg2+ and ADP the enzyme catalyzes the Ap3A synthesis via adenylate intermediate. Ap3A (not Ap4A) may serve as a s...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Merkulova T,Kovaleva G,Kisselev L

    更新日期:1994-08-22 00:00:00

  • Cloning and characterization of a root specific high-affinity sulfate transporter from Arabidopsis thaliana.

    abstract::Hst1At (accession number AB018695) was identified from the Arabidopsis thaliana sequencing project on BAC T3F12, and the corresponding cDNA was isolated by reverse transcription-PCR. Southern blot analysis reveals a single copy of this gene. The cDNA encodes a root specific sulfate transporter of 649 amino acids. Hete...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Vidmar JJ,Tagmount A,Cathala N,Touraine B,Davidian JE

    更新日期:2000-06-09 00:00:00

  • Ehhmbp45 is a novel hemoglobin-binding protein identified in Entamoeba histolytica.

    abstract::Hemoglobin-binding proteins are necessary for pathogens to obtain iron from Hb. Entamoeba histolytica can grow using Hb as source of iron, but the underlying mechanism has not previously been established. In this work, we identified a 45 kDa Hb-binding protein of E. histolytica, which we named Ehhmbp45. In silico anal...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Cruz-Castañeda A,Olivares-Trejo JJ

    更新日期:2008-08-06 00:00:00

  • Regulation of the F0F1-ATP synthase: the conformation of subunit epsilon might be determined by directionality of subunit gamma rotation.

    abstract::F(0)F(1)-ATP synthase couples ATP synthesis/hydrolysis with transmembrane proton transport. The catalytic mechanism involves rotation of the gamma epsilon c(approximately 10)-subunits complex relative to the rest of the enzyme. In the absence of protonmotive force the enzyme is inactivated by the tight binding of MgAD...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审


    authors: Feniouk BA,Junge W

    更新日期:2005-09-26 00:00:00

  • Alteration of transbilayer phospholipid compositions is involved in cell adhesion, cell spreading, and focal adhesion formation.

    abstract::We previously showed that P4-ATPases, ATP10A/ATP8B1, and ATP11A/ATP11C have flippase activities toward phosphatidylcholine (PC), and aminophospholipids [phosphatidylserine (PS) and phosphatidylethanolamine], respectively. Here, we investigate the effect of PC-specific flippases versus aminophospholipid-specific flippa...

    journal_title:FEBS letters

    pub_type: 信件


    authors: Miyano R,Matsumoto T,Takatsu H,Nakayama K,Shin HW

    更新日期:2016-07-01 00:00:00

  • The terminal respiratory chain of the methylotrophic bacterium Methylophilus methylotrophus.

    abstract::Cytochrome oxidase o has been isolated from the obligately aerobic, methylotrophic bacterium Methylophilus methylotrophus in the form of a cytochrome cL-o complex. The latter is comprised of cytochrome cL (Mr 21 000) and cytochrome o (Mr 29 000) in a 1-2:1 ratio, possibly in association with one or more minor polypept...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Carver MA,Jones CW

    更新日期:1983-05-08 00:00:00

  • Complete primary structure of calcium-dependent serine proteinase capable of degrading extracellular matrix proteins.

    abstract::A novel calcium-dependent serine proteinase (CASP) secreted from malignant hamster embryo fibroblast Ni 12C2 degrades extracellular matrix proteins. A complementary DNA encoding CASP has been isolated with the use of oligonucleotide probes synthesized based on partial amino acid sequences of CASP. The complete amino a...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Kinoshita H,Sakiyama H,Tokunaga K,Imajoh-Ohmi S,Hamada Y,Isono K,Sakiyama S

    更新日期:1989-07-03 00:00:00

  • The chloride channel blocker 5-nitro-2-(3-phenylpropyl-amino) benzoic acid (NPPB) uncouples mitochondria and increases the proton permeability of the plasma membrane in phagocytic cells.

    abstract::We present evidence that the potent chloride channel blocker NPPB has protonophoric activity in the mitochondria and across the plasma membrane of phagocytic cells. The resting O2 consumption of murine peritoneal macrophages was stimulated up to 2.5-fold in the presence of NPPB, with a K0.5 of 15 microM. The stimulato...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Lukacs GL,Nanda A,Rotstein OD,Grinstein S

    更新日期:1991-08-19 00:00:00

  • Glycinebetaine enhances and stabilizes the evolution of oxygen and the synthesis of ATP by cyanobacterial thylakoid membranes.

    abstract::Glycinebetaine (betaine), an osmoregulant in halophilic plants, stabilized the evolution of oxygen and the synthesis of ATP by thylakoid membranes from the cyanobacterium Synechocystis PCC6803 when it was present during the preparation and incubation of the thylakoid membranes. Moreover, betaine enhanced the evolution...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Mamedov MD,Hayashi H,Wada H,Mohanty PS,Papageorgiou GC,Murata N

    更新日期:1991-12-09 00:00:00

  • The nucleocapsid protein of SARS coronavirus has a high binding affinity to the human cellular heterogeneous nuclear ribonucleoprotein A1.

    abstract::The nucleocapsid (N) protein of SARS coronavirus (SARS_CoV) is a major structural component of virions, which appears to be a multifunctional protein involved in viral RNA replication and translation. Heterogeneous nuclear ribonucleoprotein A1 (hnRNP A1) is related to the pre-mRNA splicing in the nucleus and translati...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Luo H,Chen Q,Chen J,Chen K,Shen X,Jiang H

    更新日期:2005-05-09 00:00:00

  • Novel disulfide-constrained pentapeptides as models for beta-VIa turns in proteins.

    abstract::The conformational behavior of cyclic peptides of the amino acid sequence Cys-Phe/Ala-Pro-Ala-Cys has been investigated through the combined use of molecular simulation methods and NMR experiments to find models for beta-VIa turns of proteins. Both oxidized (cyclic) peptides and reduced (linear) forms were investigate...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Weisshoff H,Frost K,Brandt W,Henklein P,Mügge C,Frömmel C

    更新日期:1995-09-25 00:00:00

  • Phosphoramidon inhibits the generation of endothelin-1 from exogenously applied big endothelin-1 in cultured vascular endothelial cells and smooth muscle cells.

    abstract::When cultured porcine aortic endothelial cells (ECs) were incubated with porcine big endothelin-1 (bit ET-1(1-39)), there was a time-dependent increase in immunoreactive (IR)-ET in the culture supernatant, in addition to an endogenous IR-ET release fron the cells. Reverse-phase HPLC of the culture supernatant revealed...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Ikegawa R,Matsumura Y,Tsukahara Y,Takaoka M,Morimoto S

    更新日期:1991-11-18 00:00:00

  • Substrate interactions in cytochrome P-450: correlation between carbon-13 nuclear magnetic resonance chemical shifts and C-O vibrational frequencies.

    abstract::13CO NMR chemical shifts and 12CO infrared stretching frequencies have been measured for cytochrome P-450cam-CO in the presence of D-camphor and of various camphor analogues. A linear correlation between both parameters delta (13C) and v(CO) was found indicating that the steric and electrostatic interactions acting on...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Legrand N,Bondon A,Simonneaux G,Jung C,Gill E

    更新日期:1995-05-08 00:00:00

  • Regulation of human growth and differentiation factor 3 gene expression by NANOG in human embryonic carcinoma NCCIT cells.

    abstract::We investigated transactivation by NANOG in regulating growth and differentiation factor 3 (GDF3) expression in NCCIT cells. GDF3 expression was affected by shRNA-mediated downregulation and by exogenous overexpression of NANOG specifically, as well as by retinoic acid-mediated differentiation. GDF3 transcription was ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Park SW,Lim HY,Do HJ,Sung B,Huh SH,Uhm SJ,Song H,Chung HJ,Kim JH,Kim NH,Kim JH

    更新日期:2012-09-21 00:00:00

  • High-level production and isolation of human recombinant alpha 1-proteinase inhibitor in yeast.

    abstract::The cDNA coding for mature human alpha 1-proteinase inhibitor (alpha 1-PI) has been inserted into a variety of yeast expression vectors. Yeast cells transformed with these plasmids were then assayed for the production of mature, unglycosylated alpha 1-PI. The production level is optimal when the recombinant plasmid ca...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Hoylaerts M,Weyens A,Bollen A,Harford N,Cabezón T

    更新日期:1986-08-11 00:00:00

  • Selective inhibition of divergent seryl-tRNA synthetases by serine analogues.

    abstract::Seryl-tRNA synthetases (SerRSs) fall into two distinct evolutionary groups of enzymes, bacterial and methanogenic. These two types of SerRSs display only minimal sequence similarity, primarily within the class II conserved motifs, and possess distinct modes of tRNA(Ser) recognition. In order to determine whether the t...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Ahel D,Slade D,Mocibob M,Söll D,Weygand-Durasevic I

    更新日期:2005-08-15 00:00:00

  • Localization and in vitro binding studies suggest that the cytoplasmic/nuclear tobacco lectin can interact in situ with high-mannose and complex N-glycans.

    abstract::The possible in vivo interaction of the Nicotiana tabacum agglutinin (Nictaba) with endogenous glycoproteins was corroborated using a combination of confocal/electron microscopy of an EGFP-Nictaba fusion protein expressed in tobacco Bright Yellow-2 (BY-2) cells and biochemical analyses. In vitro binding studies demons...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Lannoo N,Peumans WJ,Pamel EV,Alvarez R,Xiong TC,Hause G,Mazars C,Van Damme EJ

    更新日期:2006-11-27 00:00:00

  • The phosphorylation at Thr 124 of simian virus 40 large T antigen is crucial for its oligomerization.

    abstract::SV40 large T antigen is phosphorylated at up to ten different amino acids clustered in an N-terminal and a C-terminal part of the polypeptide chain. The N-terminal phosphorylated residues include Ser 123 and Thr 124. We have analyzed the oligomerization, the complex formation with the cellular oncoprotein p53 and the ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Montenarh M,Müller D

    更新日期:1987-09-14 00:00:00

  • Muscle glycolipids in inherited muscular dystrophy of chickens.

    abstract::Gangliosides and neutral glycolipids of muscles from normal and dystrophic chickens were studied. Total glycolipid content of the degenerating muscles was higher than the normal muscles. In addition, the myopathic muscles contained a ganglioside which was absent in the unaffected muscles from normal and dystrophic chi...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Kundu SK,Misra LK,Luthra MG

    更新日期:1982-12-27 00:00:00

  • In vitro synthesis, tetramerization and single channel characterization of virus-encoded potassium channel Kcv.

    abstract::Chlorella virus-encoded membrane protein Kcv represents a new class of potassium channel. This 94-amino acids miniature K(+) channel consists of two trans-membrane alpha-helix domains intermediated by a pore domain that contains a highly conserved K(+) selectivity filter. Therefore, as an archetypal K(+) channel, the ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Shim JW,Yang M,Gu LQ

    更新日期:2007-03-06 00:00:00

  • Fibromodulin and lumican bind to the same region on collagen type I fibrils.

    abstract::Fibromodulin and lumican are closely related members of the extracellular matrix leucine-rich repeat glycoprotein/proteoglycan family. Similar to decorin, another member of this protein family, they bind to fibrillar collagens and function in the assembly of the collagen network in connective tissues. We have studied ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Svensson L,Närlid I,Oldberg A

    更新日期:2000-03-24 00:00:00

  • A new type of mitogenic factor produced by Streptococcus pyogenes.

    abstract::A new type of mitogenic factor (protein) was purified from the culture supernatant of a strain of Streptococcus pyogenes by SP-Sephadex C-25 column chromatography, preparative isoelectric focusing and reversed-phase high-performance liquid chromatography. The purified factor, showing marked mitogenic activity in rabbi...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Yutsudo T,Murai H,Gonzalez J,Takao T,Shimonishi Y,Takeda Y,Igarashi H,Hinuma Y

    更新日期:1992-08-10 00:00:00