Abstract:
:Cap-independent translation (CIT) occurs at the leader sequences of uncapped plant viral RNAs, but also at a number of normally capped cellular mRNAs and has been correlated with sequence complementarity to 18S rRNA. The ribosomal protein S18 (RPS18) is a component of the small ribosomal subunit and is encoded by three gene copies (A, B, and C) in the Arabidopsis thaliana genome. The RPS18C mRNA was most abundant and contained a short 5' untranslated region of 84 bp that is complementary to a novel putative interaction site at the 3' end of the 18S rRNA. The RPS18C leader mediated CIT as demonstrated by dicistronic constructs consisting of luciferase and chloramphenicol acetyl transferase reporter genes in an in vitro wheat germ extract system. CIT was rapidly inhibited upon addition of an oligonucleotide that competed for the 18S rRNA site complementary to the RPS18C leader and interfered with polysome assembly at the transcript.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Vanderhaeghen R,De Clercq R,Karimi M,Van Montagu M,Hilson P,Van Lijsebettens Mdoi
10.1016/j.febslet.2006.04.012subject
Has Abstractpub_date
2006-05-15 00:00:00pages
2630-6issue
11eissn
0014-5793issn
1873-3468pii
S0014-5793(06)00433-9journal_volume
580pub_type
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