Leader sequence of a plant ribosomal protein gene with complementarity to the 18S rRNA triggers in vitro cap-independent translation.

Abstract:

:Cap-independent translation (CIT) occurs at the leader sequences of uncapped plant viral RNAs, but also at a number of normally capped cellular mRNAs and has been correlated with sequence complementarity to 18S rRNA. The ribosomal protein S18 (RPS18) is a component of the small ribosomal subunit and is encoded by three gene copies (A, B, and C) in the Arabidopsis thaliana genome. The RPS18C mRNA was most abundant and contained a short 5' untranslated region of 84 bp that is complementary to a novel putative interaction site at the 3' end of the 18S rRNA. The RPS18C leader mediated CIT as demonstrated by dicistronic constructs consisting of luciferase and chloramphenicol acetyl transferase reporter genes in an in vitro wheat germ extract system. CIT was rapidly inhibited upon addition of an oligonucleotide that competed for the 18S rRNA site complementary to the RPS18C leader and interfered with polysome assembly at the transcript.

journal_name

FEBS Lett

journal_title

FEBS letters

authors

Vanderhaeghen R,De Clercq R,Karimi M,Van Montagu M,Hilson P,Van Lijsebettens M

doi

10.1016/j.febslet.2006.04.012

subject

Has Abstract

pub_date

2006-05-15 00:00:00

pages

2630-6

issue

11

eissn

0014-5793

issn

1873-3468

pii

S0014-5793(06)00433-9

journal_volume

580

pub_type

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