Abstract:
:Filamin plays an important role in actin cytoskeleton organization, membrane stabilization, and anchoring of transmembrane proteins. Using short interfering RNA (siRNA) to selectively target the filamin A gene and silence its expression, we studied the role of filamin A in G protein coupled receptor (GPCR) signaling. Silencing of filamin A protein expression was determined by immunoblotting and immunofluorescence. Functional consequences of filamin A gene silencing were measured by studying its role in MAPK signaling pathways activated by the Ca2+ -sensing receptor. This work defines filamin A involvement in GPCR signaling pathways and describes an additional method for studying its function.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Huang C,Wu Z,Hujer KM,Miller RTdoi
10.1016/j.febslet.2006.02.035keywords:
subject
Has Abstractpub_date
2006-03-20 00:00:00pages
1795-800issue
7eissn
0014-5793issn
1873-3468pii
S0014-5793(06)00235-3journal_volume
580pub_type
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