A simple model to explain three-base periodicity in coding DNA.

abstract：:As a conserved non-coding RNA gene, transcripts of MALAT-1 localize predominately in the nucleus. However in G2/M cell cycle phase, MALAT-1 transcripts were surprisingly found to translocate from the nucleus into the cytoplasm. Investigation also found that in this process MALAT-1 interacts with an abundant nuclear fa...

journal_title：FEBS letters

authors： Yang F,Yi F,Han X,Du Q,Liang Z

更新日期：2013-10-01 00:00:00

abstract：:Fatty acid (FA) uniport via mitochondrial uncoupling protein (UcP) was detected fluorometrically with PBFI, potassium-binding benzofuran phthalate and SPQ, 6-methoxy-N-(3-sulfopropyl)-quinolinium, indicating K+ and H+, respectively. The FA structural patterns required for FA flip-flop, UcP-mediated FA uniport, activat...

journal_title：FEBS letters

authors： Jezek P,Modrianský M,Garlid KD

更新日期：1997-05-19 00:00:00

abstract：:Mouse epidermal cytosol contains a protein phosphatase with Mr 38,000, which dephosphorylates the elongation factor 2 (EF-2) of protein biosynthesis and is stimulated after topical application of TPA to mouse skin [(1988) Biochem. Biophys. Res. Commun. 153, 1129-1135]. Dephosphorylation of EF-2 by this phosphatase is ...

journal_title：FEBS letters

authors： Gschwendt M,Kittstein W,Mieskes G,Marks F

更新日期：1989-11-06 00:00:00

abstract：:The largest mammalian enzyme family is the kinases. Kinases and other nucleotide-binding proteins are key regulators of signal transduction pathways and the mutation or overexpression of these proteins is often the difference between health and disease. As a result, a massive research effort has focused on understandi...

journal_title：FEBS letters

authors： Rosenblum JS,Nomanbhoy TK,Kozarich JW

更新日期：2013-06-27 00:00:00

abstract：:A set of inhibitors that are useful for distinction of three types of human cytosolic glutathione transferase is presented. The near-neutral transferase is inhibited most effectively by Cibacron blue (I50 = 0.05 microM), the acidic transferase by Cibacron blue (I50 = 0.5 microM), and the basic transferase by tributylt...

journal_title：FEBS letters

authors： Tahir MK,Guthenberg C,Mannervik B

更新日期：1985-02-25 00:00:00

abstract：:The determination of protein structure by NMR is restricted at molecular masses above 10 kDa by overlapping resonances. One way of overcoming this problem is to label the protein with 15N. The conventional way to record 15N spectra is to use heteronuclear multiple-quantum coherence. We present here an alternative appr...

journal_title：FEBS letters

authors： Norwood TJ,Boyd J,Campbell ID

更新日期：1989-09-25 00:00:00

abstract：:Cyanomorpholinoadriamycin (1 microM) was reacted in a transcription buffer with DNA of an initiated transcription complex. Subsequent elongation of the initiated complex revealed permanent transcriptional blockages at 16 sites after only 5 min of drug-DNA reaction time. The most dominant sites were immediately prior t...

journal_title：FEBS letters

authors： Cullinane C,Phillips DR

更新日期：1991-11-18 00:00:00

abstract：:Formation of tubulin/Na(+),K(+)-ATPase (NKA) complex in erythrocytes of hypertensive subjects results in a 50% reduction in NKA activity. We demonstrate here that detyrosinated tubulin, which is increased in hypertensive erythrocytes membranes, enhances the inhibitory effect of acetylated tubulin on NKA activity. More...

journal_title：FEBS letters

authors： Amaiden MR,Santander VS,Monesterolo NE,Nigra AD,Rivelli JF,Campetelli AN,Pie J,Casale CH

更新日期：2015-01-30 00:00:00

abstract：:Melanogenesis is an important biochemical process for the production of skin pigments which protect many animals from the damage of solar radiation. The abnormalities in melanogenesis are associated with albinism, vitiligo, as well as malignant melanoma in humans. In the lower forms of animals viz., insects, the exosk...

journal_title：FEBS letters

authors： Sugumaran M

更新日期：1991-12-16 00:00:00

abstract：:Inhibition of mammalian DNA topoisomerase I by phospholipids was investigated using purified enzyme. Acidic phospholipids inhibited the DNA relaxation activity of topoisomerase I whereas neutral phospholipid, phosphatidylethanolamine, did not. Accumulation of a protein-DNA cleavable complex, an intermediate which is k...

journal_title：FEBS letters

authors： Tamura H,Ikegami Y,Ono K,Sekimizu K,Andoh T

更新日期：1990-02-12 00:00:00

abstract：:In this study we analyzed the interaction of prion protein PrP(C) with components of glycosphingolipid-enriched microdomains in lymphoblastoid T cells. PrP(C) was distributed in small clusters on the plasma membrane, as revealed by immunoelectron microscopy. PrP(C) is present in microdomains, since it coimmunoprecipit...

journal_title：FEBS letters

authors： Mattei V,Garofalo T,Misasi R,Circella A,Manganelli V,Lucania G,Pavan A,Sorice M

更新日期：2004-02-27 00:00:00

abstract：:The kinetic properties of dUTPase from equine infectious anemia virus (EIAV) were investigated. K(M) (1.1 +/- 0.1 microM) and k(cat) (25 s(-1)) were found to be independent of pH in the neutral pH range. Above pH 8.0, K(M) increases slightly. Below pH 6.0, the enzyme is rapidly deactivated. Detergent was found to enha...

journal_title：FEBS letters

authors： Nord J,Larsson G,Kvassman JO,Rosengren AM,Nyman PO

更新日期：1997-09-08 00:00:00

abstract：:The effects of mildly acidic conditions on the free energy of unfolding (DeltaG(u)(buff)) of the pore-forming alpha-hemolysin (alphaHL) from Staphylococcus aureus were assessed between pH 5.0 and 7.5 by measuring intrinsic tryptophan fluorescence, circular dichroism and elution time in size exclusion chromatography du...

journal_title：FEBS letters

authors： Bortoleto RK,Ward RJ

更新日期：1999-10-15 00:00:00

abstract：:Single molecule localization microscopy (SMLM), which can provide up to an order of magnitude improvement in spatial resolution over conventional fluorescence microscopy, has the potential to be a highly useful tool for quantitative biological experiments. It has already been used for this purpose in varied fields in ...

journal_title：FEBS letters

authors： Shivanandan A,Deschout H,Scarselli M,Radenovic A

更新日期：2014-10-01 00:00:00

abstract：:A pumpkin 2S albumin with ribonuclease (RNase) activity was purified from pumpkin seeds (Cucurbita sp.) by liquid chromatographic techniques. It manifested potent RNase activity toward baker's yeast RNA and calf liver RNA, and some polyhomoribonucleotides, including poly(A), poly(U) and poly(C) but not poly(G). Moreov...

journal_title：FEBS letters

authors： Fang EF,Wong JH,Lin P,Ng TB

更新日期：2010-09-24 00:00:00

abstract：:Since the discovery of RNAi, its mechanism in plants and animals has been intensively studied, widely exploited as a research tool, and used for a number of potential commercial applications. In this article, we discuss the platforms for delivering RNAi in plants. We provide a brief background to these platforms and c...

journal_title：FEBS letters

authors： Watson JM,Fusaro AF,Wang M,Waterhouse PM

更新日期：2005-10-31 00:00:00

abstract：:Protein kinase C (PKC) may be involved in growth regulation. In the present study the relationship between body weight, and thereby age, and the activity of PKC in muscle as well as in rapidly growing overloaded muscle were investigated. PKC activity in music was linearly inversely correlated to rat weight in both sol...

journal_title：FEBS letters

authors： Richter EA,Nielsen NB

更新日期：1991-09-02 00:00:00

abstract：:In this review, we summarize the currently available information on the membrane topology of some key members of the human ABC protein subfamilies, and present the predicted domain arrangements. In the lack of high-resolution structures for eukaryotic ABC transporters this topology is based only on prediction algorith...

journal_title：FEBS letters

authors： Tusnády GE,Sarkadi B,Simon I,Váradi A

更新日期：2006-02-13 00:00:00

abstract：:The addition of 5-azacytidine to erythroleukemic cells which were induced to differentiate with DMSO or BA altered the expression of the hemoglobins. After the addition of 5-azacytidine there was an increase in hemoglobin synthesis especially in the embryonic E2 band. The beta-globin increased in synthesis after 5-aza...

journal_title：FEBS letters

authors： Zucker RM,Decal DL,Whittington KB

更新日期：1983-10-17 00:00:00

abstract：:Hepatitis C Virus core protein (HCVc) plays important roles in the development of intrahepatic cholangiocarcinoma (ICC). MicroRNAs (miRNAs) contribute to tumor progression by interacting with downstream target genes. However, the regulation and role of miRNAs in HCV-related intrahepatic cholangiocarcinoma (HCV-ICC) is...

journal_title：FEBS letters

authors： Zeng B,Li Z,Chen R,Guo N,Zhou J,Zhou Q,Lin Q,Cheng D,Liao Q,Zheng L,Gong Y

更新日期：2012-09-21 00:00:00

abstract：:The ANKRD1/CARP gene encodes a muscle-specific protein which has been implicated in transcriptional regulation and myofibrillar assembly. Several features at both the mRNA and protein levels define ANKRD1 as a gene whose expression is tightly regulated, and deregulated expression of this protein has been recently asso...

journal_title：FEBS letters

authors： Badi I,Cinquetti R,Frascoli M,Parolini C,Chiesa G,Taramelli R,Acquati F

更新日期：2009-08-06 00:00:00

abstract：:By co-expression of heme oxygenase and various bilin reductase(s) in a single operon in conjunction with apophytochrome using two compatible plasmids, we developed a system to produce phytochromes with various chromophores in Escherichia coli. Through the selection of different bilin reductases, apophytochromes were a...

journal_title：FEBS letters

authors： Mukougawa K,Kanamoto H,Kobayashi T,Yokota A,Kohchi T

更新日期：2006-02-20 00:00:00

abstract：:Saccharomyces cerevisiae has only one gene encoding a putative voltage-gated Ca2+ channel pore-forming subunit, CCH1, which is not possible to be cloned by conventional molecular cloning techniques using Escherichia coli. Here, we report the successful cloning of CCH1 in yeast by in vivo homologous recombination witho...

journal_title：FEBS letters

authors： Iida K,Tada T,Iida H

更新日期：2004-10-22 00:00:00

abstract：:The regulation of genes for acetylcholine receptor (AChR), myogenic factors and other muscle-specific proteins has been analyzed in experimental autoimmune myasthenia gravis (EAMG) and following denervation. The levels of the transcripts for the myogenic factors, MyoD1, myogenin and MRF4, were measured using Northern ...

journal_title：FEBS letters

authors： Asher O,Fuchs S,Zuk D,Rapaport D,Buonanno A

更新日期：1992-03-24 00:00:00

abstract：:We have set up a model system for familial amyotrophic lateral sclerosis (FALS) by transfecting human neuroblastoma cell line SH-SY5Y with plasmids directing constitutive expression of either wild-type human Cu,Zn superoxide dismutase (Cu,ZnSOD) or a mutant of this enzyme (G93A) associated with FALS. We have tested mi...

journal_title：FEBS letters

authors： Carrì MT,Ferri A,Battistoni A,Famhy L,Gabbianelli R,Poccia F,Rotilio G

更新日期：1997-09-08 00:00:00

abstract：:The essential role of Factor VIII:C (FVIII:C, anti-hemophilia factor A) as a cofactor for Factor IXa-dependent activation of Factor X has been established. In this paper, we describe that capillary endothelial cells from bovine adrenal medulla express active FVIII:C gene. Accumulation of FVIII:C in conditioned media f...

journal_title：FEBS letters

authors： Banerjee DK,Tavárez JJ,Oliveira CM

更新日期：1992-07-13 00:00:00

abstract：:In Alzheimer's disease, beta-amyloid peptides (betaA(1-40) and betaA(1-42)) are deposited on the brain cell surfaces as neurotoxic plaques. Some reports indicate that small heat shock proteins, Hsp27 and alphaB-crystallin, colocalize in the plaques, but their functions are not known. Interaction between betaA and alph...

journal_title：FEBS letters

authors： Liang JJ

更新日期：2000-11-03 00:00:00

abstract：:The stomach form of alcohol dehydrogenase has been structurally evaluated by peptide analysis covering six separate regions of the rat enzyme. Overall, this new structure differs widely (32-40% residue differences) from the structures of three classes of alcohol dehydrogenase characterized before from the same species...

journal_title：FEBS letters

authors： Parés X,Moreno A,Cederlund E,Höög JO,Jörnvall J

更新日期：1990-12-17 00:00:00

abstract：:Upon dimerization by oxidation, Hsp33 functions as a molecular chaperone in prokaryotes. Previously published structures of both the inactive and active species are of doubtful relevance to the solution conformations since the inactive (reduced) crystal structure was dimeric, while the active (oxidized) species was cr...

journal_title：FEBS letters

authors： Lee YS,Ryu KS,Kim SJ,Ko HS,Sim DW,Jeon YH,Kim EH,Choi WS,Won HS

更新日期：2012-02-17 00:00:00

abstract：:The Hippo-Warts pathway defines a novel signaling cascade involved in organ size control and tumor suppression. However, the developmental function of this pathway is less understood. Here we report that the Caenorhabditis elegans homolog of Warts, Ce-wts-1, plays important roles during worm development. The null alle...

journal_title：FEBS letters

authors： Cai Q,Wang W,Gao Y,Yang Y,Zhu Z,Fan Q

更新日期：2009-10-06 00:00:00