Regulation of [Ca(2+)](i) homeostasis in MRP1 overexpressing cells.

abstract：:A 32 kDa phospholipase A2 inhibitory protein was isolated from pig thyroid gland after calcium precipitation and fast protein liquid anion-exchange chromatography. SDS-polyacrylamide gel electrophoresis revealed the purity of the protein. The protein activity was assessed by the inhibition of pancreatic phospholipase ...

journal_title：FEBS letters

authors： Antonicelli F,Rothhut B,Martiny L,Aguie-Aguie G,Lambert B,Bellon G,Russo-Marie F,Jacquemin C,Haye B

更新日期：1988-08-01 00:00:00

abstract：:Sodium azide inhibited multi-site (steady-state) ATPase activity of E. coli F1 more than 90%, but did not affect uni-site (single-site) ATPase activity. Thus azide inhibited multi-site ATPase activity by lowering catalytic cooperativity. Consistent with this observation, azide changed the ligand-induced fluorescence r...

journal_title：FEBS letters

authors： Noumi T,Maeda M,Futai M

更新日期：1987-03-23 00:00:00

abstract：:hTrp3 is a human homologue of the Drosophila gene responsible for a transient receptor potential (trp) mutation. When stably expressed in HEK293 cells, hTrp3 formed ion channels that were active under resting conditions but could be further stimulated by carbachol or ATP via endogenous muscarinic or purinergic recepto...

journal_title：FEBS letters

authors： Hurst RS,Zhu X,Boulay G,Birnbaumer L,Stefani E

更新日期：1998-02-06 00:00:00

abstract：:Cerebellar granule cells differentiated in culture were incubated with ganglioside [3H-Sph]GM1 in order to have it inserted into the plasma membrane and metabolized. Among the formed metabolites radioactive sphingosine and ceramide were identified. [3H]Ceramide started to be measurable after 10 min of incubation (puls...

journal_title：FEBS letters

authors： Riboni L,Bassi R,Sonnino S,Tettamanti G

更新日期：1992-03-30 00:00:00

abstract：:Human immunodeficiency virus type 1 (HIV-1) Rev is a small RNA-binding protein which is essential for viral replication. To investigate the structure of Rev we have mapped the binding sites of a panel of monoclonal antibodies (mAb) by protein footprinting and identified a mAb protecting amino acids within both the N- ...

journal_title：FEBS letters

authors： Jensen TH,Jensen A,Szilvay AM,Kjems J

更新日期：1997-09-01 00:00:00

abstract：:Lipoic acid is a coenzyme essential to the activity of enzymes such as pyruvate dehydrogenase, which play important roles in central metabolism. However, neither the enzymes responsible for biosynthesis nor the biosynthetic event of lipoic acid has been reported in mammalian cells. In this study, a mouse mLIP1 cDNA fo...

journal_title：FEBS letters

authors： Morikawa T,Yasuno R,Wada H

更新日期：2001-06-01 00:00:00

abstract：:Biotops with extreme temperatures such as deserts force animals to avoid or escape high temperatures by biochemical, behavioural or morphological adaptation. In this context we tested the resistance to heat of the oxygen carrier hemocyanin from the ancient tarantula Eurypelma californicum, which is found in arid zones...

journal_title：FEBS letters

authors： Sterner R,Vogl T,Hinz HJ,Penz F,Hoff R,Föll R,Decker H

更新日期：1995-05-01 00:00:00

abstract：:Hterologous complementation of yeast mutants has enabled the isolation of genes encoding several families of amino acid transporters. Among them, NTR1 codes for a membrane protein with weak histidine transport activity. However, at the sequence level, NTR1 is related to rather non-specific oligopeptide transporters fr...

journal_title：FEBS letters

authors： Rentsch D,Laloi M,Rouhara I,Schmelzer E,Delrot S,Frommer WB

更新日期：1995-08-21 00:00:00

abstract：:We investigated the role of p53 in nuclear factor (NF)-kappaB dependent, platelet-activating factor (PAF)-induced vascular endothelial growth factor (VEGF) expression. Transfected NF-kappaB subunits in ECV304 cells increased the tumor necrosis factor-alpha promoter activity, which was completely inhibited by p53. Tran...

journal_title：FEBS letters

authors： Ko HM,Jung HH,Seo KH,Kang YR,Kim HA,Park SJ,Lee HK,Im SY

更新日期：2006-05-29 00:00:00

abstract：:Chronic inflammation associated with tumor necrosis factor (TNF)-alpha and reactive oxygen species (ROS) is the hallmark of tuberculosis. Mycobacterium tuberculosis (MTB) directly stimulates human monocytes to secrete TNF-alpha. We show the augmented expression of TNF-alpha mRNA in MTB-infected monocytes by cellular a...

journal_title：FEBS letters

authors： Hasan N,Yusuf N,Toossi Z,Islam N

更新日期：2006-05-01 00:00:00

abstract：:The inhibitory glycine receptor (GlyR) is a ligand-gated chloride channel protein which displays developmental heterogeneity in the mammalian central nervous system. Here we describe 2 novel cDNA variants of the rat GlyR alpha 2 subunit and demonstrate that alternative splicing generates these 2 isoforms. The deduced ...

journal_title：FEBS letters

authors： Kuhse J,Kuryatov A,Maulet Y,Malosio ML,Schmieden V,Betz H

更新日期：1991-05-20 00:00:00

abstract：:(1-->3)-alpha-glucanases catalyze the hydrolysis of fungal cell wall (1-->3)-alpha-glucan, and function during cell division of yeasts containing this cell wall component or act in mycoparasitic processes. Here, we characterize the mechanism of action of the (1-->3)-alpha-glucanase MutAp from the mycoparasitic fungus ...

journal_title：FEBS letters

authors： Grün CH,Dekker N,Nieuwland AA,Klis FM,Kamerling JP,Vliegenthart JF,Hochstenbach F

更新日期：2006-07-10 00:00:00

abstract：:We employed cDNA representational difference analysis to identify new genes that are upregulated by 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) in a human hepatoblastoma cell line HepG2. We isolated several TCDD-responsive cDNAs. Sequence analysis revealed that one of them encodes E16/CD98LC/hLAT1, an integral membrane...

journal_title：FEBS letters

authors： Sarkar D,Kambe F,Hirata A,Iseki A,Ohmori S,Seo H

更新日期：1999-12-03 00:00:00

abstract：:Sarcoplasmic reticulum vesicles were solubilized stepwise by the nonionic detergent octaethyleneglycol monododecyl ether; 31P NMR enabled the extent of phospholipid solubilization to be monitored by following the conversion of the broad resonance peak characterizing the phospholipids inserted in the bilayer to the nar...

journal_title：FEBS letters

authors： Roux M,Champeil P

更新日期：1984-06-11 00:00:00

abstract：:African horse sickness virus (AHSV), a member of the orbivirus genus of the family Reoviridae, is an insect-vectored pathogen of horses of concern to the equine industry. Studies on AHSV replication and pathogenesis have been hampered by the lack of reverse genetics allowing targeted mutation of viral genomes. We demo...

journal_title：FEBS letters

authors： Matsuo E,Celma CC,Roy P

更新日期：2010-08-04 00:00:00

abstract：:Unlike the human blood group Sd(a+) Tamm-Horsfall glycoprotein (THGP), the Sd(a-) one lacks terminal GalNAcbeta1--> residues at the nonreducing ends. The binding properties of this glycoprotein and its asialo product with lectins were characterized by quantitative precipitin (QPA) and precipitin inhibition assays. Amo...

journal_title：FEBS letters

authors： Wu JH,Watkins WM,Chen CP,Song SC,Wu AM

更新日期：1996-04-22 00:00:00

abstract：:By sequence analysis we show that the U104 domain found in the UNC104 subfamily of kinesins is a forkhead homology-associated domain (FHA). A combination of limited proteolysis, mass spectroscopy, and physicochemical analysis define this domain as a genuine autonomously folding domain. Our data show that the FHA domai...

journal_title：FEBS letters

authors： Westerholm-Parvinen A,Vernos I,Serrano L

更新日期：2000-12-15 00:00:00

abstract：:The phosphatidylinositol 4,5-bisphosphate (PtdIns-P2)-regulated actin-binding protein gelsolin and most phosphoinositide-specific phospholipases C (PLCs) comprise a basic amino acid motif ((K/R)xxxKxK(K/R); x denotes any amino acid) which was previously suggested to represent a PtdInsP2-binding site commonly present i...

journal_title：FEBS letters

authors： Simões AP,Camps M,Schnabel P,Gierschik P

更新日期：1995-05-29 00:00:00

abstract：:Membrane incorporation and aggregation of the peptide alamethicin have been investigated as a function of lipid type. Head group and acyl chain regions both contribute to modulate alamethicin incorporation. Specifically, the peptide prefers thin membranes and saturated chains; incorporation is reduced by the presence ...

journal_title：FEBS letters

authors： Stankowski S,Schwarz G

更新日期：1989-07-03 00:00:00

abstract：:In temperature-induced Triton X-114 phase separation experiments the beta-glucan elicitor-binding site from soybean (Glycine max L.) root membranes was identified as (a) hydrophobic membrane protein(s). The Zwittergent 3-12-solubilized beta-glucan-binding proteins were incorporated into lipid vesicles by the detergent...

journal_title：FEBS letters

authors： Mithöfer A,Ebel J

更新日期：1999-09-17 00:00:00

abstract：:Incubation of isolated rat liver nuclei with ATP, NAD+, and micromolar Ca2+ concentrations of various metal ions resulted in extensive DNA hydrolysis. Half-maximal activity occurred with 1.0 microM Ca2+ added, and saturation of the process was observed with 10 microM Ca2+. The Ca2+ (10 microM)-activated DNA fragmentat...

journal_title：FEBS letters

authors： Yamaguchi M,Sakurai T

更新日期：1991-02-25 00:00:00

abstract：:Hepatitis C virus (HCV) replication and pathogenesis involve both virus-encoded proteins and cellular factors. In our study, we showed that NS5B, the HCV RNA-dependent RNA polymerase, interacted with M2 type pyruvate kinase (M2PK) but not L type pyruvate kinase. We confirmed the interaction by GST pull down, coimmunop...

journal_title：FEBS letters

authors： Wu X,Zhou Y,Zhang K,Liu Q,Guo D

更新日期：2008-06-25 00:00:00

abstract：:Proteorhodopsin (PR), a retinal-containing seven transmembrane helix protein, functions as a light-driven proton pump. Using PCR, we isolated 18 PR variants originating from the surface of the Arctic Ocean. Their absorption maxima were between 517 and 546 nm at pH 7. One of the isolates turned out to be identical to G...

journal_title：FEBS letters

authors： Jung JY,Choi AR,Lee YK,Lee HK,Jung KH

更新日期：2008-05-28 00:00:00

abstract：:Aah VI was isolated from the venom of the North African scorpion, Androctonus australis hector. It is the first glycosylated neurotoxin from scorpion venom to be described. It was not toxic to mice, when injected intracerebroventricularly at a dose of 1.2 microg per animal. However, it had typical activity in Blatella...

journal_title：FEBS letters

authors： Hassani O,Loew D,Van Dorsselaer A,Papandréou MJ,Sorokine O,Rochat H,Sampieri F,Mansuelle P

更新日期：1999-01-25 00:00:00

abstract：:An efficient sensing of danger and a rapid activation of the immune system are crucial for the survival of plants. Conserved pathogen/microbe-associated molecular patterns (PAMPs/MAMPs) and endogenous molecular patterns, which are present only when the tissue is infected or damaged (damage-associated molecular pattern...

journal_title：FEBS letters

authors： De Lorenzo G,Brutus A,Savatin DV,Sicilia F,Cervone F

更新日期：2011-06-06 00:00:00

abstract：:The tetracycline-controlled transcription system has become one of the most potent systems for experimental manipulations of transcription levels in vivo. Here we report on rtTA variants, which were generated by combining the existing positively regulated Tet repressor domains of rtTA and rtTA-M2 with a modified and m...

journal_title：FEBS letters

authors： Kämper MR,Gohla G,Schlüter G

更新日期：2002-04-24 00:00:00

abstract：:Mouse interferon (alpha + beta) given to mice by intraperitoneal injection suppressed both the accumulation of putrescine and stimulation of DNA synthesis in liver caused by partial hepatectomy. The suppression of DNA synthesis was completely reversed by exogenous putrescine. The same results were obtained when core 2...

journal_title：FEBS letters

authors： Nishiguchi S,Otani S,Matsui-Yuasa I,Morisawa S,Monna T,Kuroki T,Kobayashi K,Yamamoto S

更新日期：1986-09-01 00:00:00

abstract：:A spectroscopic study of the flavocytochrome b enzyme, cellobiose oxidase, employing optical, NMR, EPR and near infra-red MCD techniques, has identified the axial ligands of the b-type haem. These are a histidine and a methionine, and this ligation set is discussed in relation to the functional role of the haem group....

journal_title：FEBS letters

authors： Cox MC,Rogers MS,Cheesman M,Jones GD,Thomson AJ,Wilson MT,Moore GR

更新日期：1992-07-28 00:00:00

abstract：:The activity of the Ca-pumping ATPase of cardiac sarcoplasmic reticulum (SR) is controlled by the phosphorylation of the intrinsic regulatory protein phospholamban (PLB), which affects both the apparent Km(Ca) and the Vmax of the transport process. We have investigated the correlation between phosphorylation of PLB an...

journal_title：FEBS letters

authors： Chiesi M,Schwaller R

更新日期：1989-02-13 00:00:00

abstract：:We have analyzed the specificity of the actinomycin D-DNA interaction. The 'footprint' method has been used in this investigation. It is shown that: (i) The presence of dinucleotide GC or GG is required for binding of a single drug molecule. (ii) The strong binding sites are encoded by tetranucleotide XGCY; where X no...

journal_title：FEBS letters

authors： Scamrov AV,Beabealashvilli RS

更新日期：1983-11-28 00:00:00