Abstract:
:Incubation of isolated rat liver nuclei with ATP, NAD+, and micromolar Ca2+ concentrations of various metal ions resulted in extensive DNA hydrolysis. Half-maximal activity occurred with 1.0 microM Ca2+ added, and saturation of the process was observed with 10 microM Ca2+. The Ca2+ (10 microM)-activated DNA fragmentation was inhibited by the presence of Ca2(+)-binding protein regucalcin isolated from rat liver cytosol. The inhibitory effect of regucalcin was complete at 0.5 microM. At 25 microM Ca2+ added, such an effect of regucalcin (1.0 microM) was not seen. Regucalcin also inhibited Ca2(+)-activated DNA fragmentation in the presence of calmodulin (10 and 20 micrograms). The results show that regucalcin can inhibit the Ca2(+)-activated DNA fragmentation due to binding the metal, suggesting a role in regulation of liver nuclear functions.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Yamaguchi M,Sakurai Tdoi
10.1016/0014-5793(91)80168-3subject
Has Abstractpub_date
1991-02-25 00:00:00pages
281-4issue
2eissn
0014-5793issn
1873-3468pii
0014-5793(91)80168-3journal_volume
279pub_type
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