Abstract:
:The major mono- and disialogangliosides of the extensively characterized established human glioma line D54MG were isolated and purified from subcutaneous solid xenografts grown in athymic (nu/nu) mice. Structural determination showed that they belonged to the lactotetraosylceramide series. The sialyllactotetraosylceramide contained 90% N-glycolyl- and 10% N-acetylneuraminic acid linked in an alpha 2-3 linkage (IV3NeuGc-LcOse4Cer, IV3NeuAc-LcOse4Cer). The disialogangliosides had a previously undescribed type of structure with sialic acids linked to the terminal galactose in an alpha 2-3 linkage and to N-acetylglucosamine in an alpha 2-6 linkage. Not only did species with NeuAc or NeuGc occur, but also species with mixtures of the two sialic acids, e.g. NeuAc and NeuGc. The schematic structures of the new disialogangliosides are (Formula:see text).
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Månsson JE,Fredman P,Bigner DD,Molin K,Rosengren B,Friedman HS,Svennerholm Ldoi
10.1016/0014-5793(86)80580-4subject
Has Abstractpub_date
1986-05-26 00:00:00pages
109-13issue
1eissn
0014-5793issn
1873-3468pii
0014-5793(86)80580-4journal_volume
201pub_type
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