Abstract:
:Ribonuclease T1 can be unfolded and refolded without forming noticeable amounts of aggregates allowing to characterise the dimensions of a protein in different denatured states in terms of the Stokes radius RS. Upon thermal unfolding RS increases from 1.74 nm at 20 degrees C to 2.14 nm at 60 degrees C. By contrast, RS = 2.40 nm was obtained at 5.3 M guanidinium chloride (GuHCl) and 20 degrees C. Heating from 20 degrees C to 70 degrees C in the presence of 5.3 M GuHCl led to a 5% decrease in RS.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Gast K,Zirwer D,Damaschun H,Hahn U,Müller-Frohne M,Wirth M,Damaschun Gdoi
10.1016/s0014-5793(97)00058-6subject
Has Abstractpub_date
1997-02-24 00:00:00pages
245-8issue
3eissn
0014-5793issn
1873-3468pii
S0014-5793(97)00058-6journal_volume
403pub_type
杂志文章相关文献
FEBS LETTERS文献大全abstract::Phosphorylation of the 20 kDa myosin light chain from smooth muscle by five different kinases was investigated. Three of the kinases (myosin light chain kinase, phosphorylase kinase, and cAMP-dependent protein kinase) phosphorylate serine residues, the fourth (casein-kinase-2) mainly threonine, and the fifth (glycogen...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(83)80449-9
更新日期:1983-08-08 00:00:00
abstract::Galectin-3, with a wide tissue distribution and marked developmental regulation, provides significant insights into the progression of various disease and developmental stages. Recognized by its specificity for galactose, a detailed characterization of its sugar binding ability has been investigated by isothermal titr...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(01)02586-8
更新日期:2001-06-29 00:00:00
abstract::Chloride intracellular channel proteins (CLICs) are distinct from most ion channels in that they have both soluble and integral membrane forms. CLICs are highly conserved in chordates, with six vertebrate paralogues. CLIC-like proteins are found in other metazoans. CLICs form channels in artificial bilayers in a proce...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2010.01.027
更新日期:2010-05-17 00:00:00
abstract::Mitochondria continually fuse and divide to dynamically adapt to changes in metabolism and stress. Mitochondrial dynamics are also required for mitochondrial DNA (mtDNA) integrity; however, the underlying reason is not known. In this study, we examined the link between mitochondrial fusion and mtDNA maintenance in Sch...
journal_title:FEBS letters
pub_type: 信件
doi:10.1002/1873-3468.12432
更新日期:2016-10-01 00:00:00
abstract::Muscle phosphofructokinase interacts with microtubule-associated protein-free microtubules resulting in a reduction of the overall activity of the enzyme [Lehotzky et al. (1993) J. Biol. Chem. 268, 10888-10894] and periodical cross-linking of the tubules [Lehotzky et al. (1994) Biochem. Biophys. Res. Commun. 204, 585-...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(95)01510-8
更新日期:1996-01-29 00:00:00
abstract::5-(1-Azidovinyl)-2'-deoxyuridine (AzVDU) and a series of 5-[1-azido-2-halogenoethyl]-derivatives of beta-D-arabinofuranosyluracil (AU) proved markedly inhibitory to the replication of herpes simplex virus type 1 (HSV-1) and varicella zoster virus (VZV), but not thymidine kinase (TK)-deficient HSV-1 and VZV strains. No...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(95)00994-k
更新日期:1995-10-02 00:00:00
abstract::We have purified and partially sequenced two proteins from Xenopus laevis previtellogenic oocytes, belonging to messenger ribonucleoprotein particles (mRNPs). The purification procedure rests on the thermostability of these proteins, which remain soluble after heating the cell extracts at 80 degrees C. The thermostabl...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(91)80456-d
更新日期:1991-04-22 00:00:00
abstract::The low density lipoprotein receptor-related protein (LRP) is a multifunctional cell surface receptor highly expressed in human aortic smooth muscle cells. In the present study, we used the short interfering RNA (siRNA) technique to explore the role of LRP in smooth muscle cell migration. We identified an LRP-specific...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(03)01272-9
更新日期:2003-12-04 00:00:00
abstract::Soluble aluminium (Al(3+)) is the major constraint to plant growth on acid soils. Plants have evolved mechanisms to tolerate Al(3+) and one type of mechanism relies on the efflux of organic anions that protect roots by chelating the Al(3+). Al(3+) resistance genes of several species have now been isolated and found to...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2007.03.057
更新日期:2007-05-25 00:00:00
abstract::Rhizobium sigma vegetative-dependent promoters are different from those of enteric bacteria and have never been characterized before. We report here the biochemical characterization of the ORF1-ntrBC promoter of Rhizobium etli. The minimal promoter region was located by means of a transcriptional fusion and further ch...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(96)00507-8
更新日期:1996-06-10 00:00:00
abstract::The conformational behavior of cyclic peptides of the amino acid sequence Cys-Phe/Ala-Pro-Ala-Cys has been investigated through the combined use of molecular simulation methods and NMR experiments to find models for beta-VIa turns of proteins. Both oxidized (cyclic) peptides and reduced (linear) forms were investigate...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(95)00982-f
更新日期:1995-09-25 00:00:00
abstract::After initial purification of ovine placental lactogen (oPL) using the procedures described previously [(1976) Endocrinology 98, 65-75], the oPL preparation was further purified by high-performance liquid chromatography (HPLC) using an anionic exchange column (Bio-Sil TSK DEAE-2-SW). Two forms of oPL with different re...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(86)80491-4
更新日期:1986-04-21 00:00:00
abstract::The phorbol esters are among the most potent tumor promoters. On addition of 12-O-tetradecanoylphorbol 13-acetate (TPA) to isolated human platelets prelabelled with [32P]orthophosphate we found a rapid increase in 32P incorporation into phosphatidylinositol 4-phosphate and phosphatidylinositol 4,5-bisphosphate. In vie...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(84)80811-x
更新日期:1984-08-06 00:00:00
abstract::Cyclic AMP-mediated phosphorylation of calcium channel subunits was studied in vitro and in vivo in preparations from dog heart. Calcium channels in native cardiac membranes were phosphorylated by cAMP-dependent protein kinase (PKA) solubilized with digitonin and subsequently immunoprecipitated using a polyclonal anti...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)80733-b
更新日期:1993-12-06 00:00:00
abstract::We have raised monoclonal antibodies (Mab) to the Mr 55,000 desmin polypeptide, electrophoretically purified from cytoskeletal preparations of isolated bovine heart Purkinje fibers. One of the Mabs, 39AB6, revealed desmin only in cow Purkinje fibers and did not react with desmins from other muscle cells, including ven...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(90)80317-c
更新日期:1990-07-02 00:00:00
abstract::We have characterized the Bacillus subtilis homologs of fructoselysine 6-kinase and fructoselysine-6-phosphate deglycase, two enzymes that specifically metabolize the Amadori compound fructose-epsilon-lysine in Escherichia coli. The B. subtilis enzymes also catalyzed the phosphorylation of fructosamines to fructosamin...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2004.10.049
更新日期:2004-11-19 00:00:00
abstract::Coagulation factor VII contains ten gamma-carboxyglutamic acid residues in the N-terminal region (Gla-domain) which are essential for the hemostatic function of FVII. The present study shows that granulocyte cathepsin G degrades the Gla-domain of FVIIa in vitro. Characterization of the truncated FVIIa by SDS-PAGE and ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(92)80989-t
更新日期:1992-07-20 00:00:00
abstract::A sensitive and specific radioimmunoassay suitable for direct measurement of leukotriene C4 was developed. Acetylated leukotriene C4 was coupled to polyamino bovine serum albumin using 1-ethyl-3-(3-dimethylamino-propyl) carbodiimide hydrochloride as coupling agent. The conjugate in complete or incomplete Freund's adju...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(83)80487-6
更新日期:1983-02-07 00:00:00
abstract::Treatment of U937 cells with dolichyl phosphate led to an increase in the activity of the ICE family protease CPP32, accompanied with cleavage of pre-CPP32 to generate p17. Peptide inhibitors YVAD-cmk and Z-Asp-CH2-DCB (specific to ICE) and DEVD-CHO (specific to CPP32) blocked the dolichyl phosphate-induced apoptosis....
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)00763-1
更新日期:1997-07-21 00:00:00
abstract::alpha-Allenyl putrescine (5,6-heptadiene-1,4-diamine) was designed as a new potential enzyme-activated irreversible inhibitor of ornithine decarboxylase (ODC). This compound, and more specifically its (R)-enantiomer, produced time-dependent inhibitions of E. coli and rat liver ODC. The inhibitions exhibit saturation k...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(84)81172-2
更新日期:1984-09-03 00:00:00
abstract::The dual function of the regulatory beta-subunit of protein kinase CK2 is highlighted by its ability to abolish calmodulin phosphorylation in contrast to its stimulatory effect on the phosphorylation of peptide substrates. Here we show that a synthetic peptide reproducing the C-terminal region of the beta-subunit (bet...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(95)00295-k
更新日期:1995-04-17 00:00:00
abstract::In adipose cells, insulin induces the translocation of GLUT4 by stimulating their exocytosis from a basal intracellular compartment to the plasma membrane. Increasing overexpression of a hemagglutinin (HA) epitope-tagged GLUT4 in rat adipose cells results in a roughly proportional increase in cell surface HA-GLUT4 lev...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)01369-1
更新日期:1999-10-29 00:00:00
abstract::Retinal dehydrogenase (RALDH) catalyzes the oxidation of retinal to all-trans and 9-cis retinoic acid, which function as ligands controlling RAR and RXR nuclear receptor-signaling pathways. We have recently shown the expression of RALDH transcript in the stomach and small intestine by reverse transcription polymerase ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)00355-x
更新日期:1998-04-17 00:00:00
abstract::To date the only tRNAs containing nucleosides modified with a selenium (5-carboxymethylaminomethyl-2-selenouridine and 5-methylaminomethyl-2-selenouridine) have been found in bacteria. By using tRNA anticodon-stem-loop fragments containing S2U, Se2U, or geS2U, we found that in vitro tRNA 2-selenouridine synthase (SelU...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1002/1873-3468.13124
更新日期:2018-07-01 00:00:00
abstract::The relevance of MDR-1 gene expression to the multidrug resistance phenotype was investigated. Drug-resistant cells, KB-V1 and MCF7/ADR, constantly expressed mRNA of the MDR-1 gene and were more resistant to vinblastine and adriamycin than drug-sensitive cells, KB-3-1 and MCF7. The drug efflux rate of KB-V1 was the sa...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)00777-1
更新日期:1997-07-21 00:00:00
abstract::The Rho-guanine nucleotide exchange factors (Rho-GEFs) remodel the actin cytoskeleton via their Rho-GTPase targets and affect numerous physiological processes such as transformation and cell motility. They are therefore attractive targets to design specific inhibitors that may have therapeutic applications. Trio conta...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(02)02928-9
更新日期:2002-07-17 00:00:00
abstract::Two fatty acid ethyl ester (FAEE) synthase isoenzymes purified from human myocardium were reported to be glutathione S-transferases (GST) [(1989) Proc. Natl. Acad. Sci. USA 86, 4470-4473; and (1989) J. Clin. Invest. 84, 1942-1946]. In the present study, the FAEE synthase activity of several purified and well character...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(90)81438-t
更新日期:1990-11-26 00:00:00
abstract::The R2 protein component of mouse ribonucleotide reductase has been obtained from overproducing Escherichia coli bacteria. It has been crystallized using NaCl as precipitant. The crystals are orthorhombic, space group C222(1), with cell dimensions a = 76.9 A, b = 108.9 A, c = 92.7 A and diffract to at least 2.5 A. The...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(95)01067-o
更新日期:1995-10-16 00:00:00
abstract::3C-like (3CL) protease is essential for the life cycle of severe acute respiratory syndrome-coronavirus (SARS-CoV) and therefore represents a key anti-viral target. A compound library consisting of 960 commercially available drugs and biologically active substances was screened for inhibition of SARS-CoV 3CL protease....
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2004.08.015
更新日期:2004-09-10 00:00:00
abstract::Plasma high density lipoprotein (HDL)-cholesterol levels are inversely correlated with the incidence of cardiovascular diseases. HDL is mainly assembled in the liver through the ATP-binding cassette transporter (ABCA1) pathway. In humans, plasma HDL-cholesterol levels are positively correlated with plasma adiponectin ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2007.09.038
更新日期:2007-10-30 00:00:00