The cytostatic activity of 5-(1-azidovinyl)-2'-deoxyuridine (AzVDU) against herpes simplex virus thymidine kinase gene-transfected FM3A cells is due to inhibition of thymidylate synthase and enhanced by UV light (lambda = 254 nm) exposure.


:5-(1-Azidovinyl)-2'-deoxyuridine (AzVDU) and a series of 5-[1-azido-2-halogenoethyl]-derivatives of beta-D-arabinofuranosyluracil (AU) proved markedly inhibitory to the replication of herpes simplex virus type 1 (HSV-1) and varicella zoster virus (VZV), but not thymidine kinase (TK)-deficient HSV-1 and VZV strains. None of the compounds were cytostatic. However, AzVDU, but not the 5-[1-azido-2-halogenoethyl]-AU derivatives became highly cytostatic against HSV-1 and HSV-2 TK gene-transfected FM3A tumor cells. The molecular target for the cytostatic effect of AzVDU proved to be thymidylate synthase. Short exposure of AzVDU-treated FM3A TK-/HSV-1 TK+ cells to irradiation at lambda = 254 nm enhanced the cytostatic activity of AzVDU by 5-fold.




FEBS letters


Balzarini J,Andrei G,Kumar R,Knaus EE,Wiebe LI,De Clercq E




Has Abstract


1995-10-02 00:00:00














  • Chromatin proteins surrounding the d(G-T)n repeats and polyamine influence as revealed by photoaffinity labeling with reactive pd(A-C)6 derivatives.

    abstract::The complementary-addressed modification of DNA and proteins in chromatin using photoreactive derivatives of pd(AC)6 has been studied. These oligonucleotides form complementary complexes with specific DNA sequences and modify both DNA and proteins in the vicinity of these regions, and can be used for investigation of ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Bozhenok LN,Khazina EB,Chernolovskaya EL,Kobets ND

    更新日期:1998-11-27 00:00:00

  • The composition of the RNA polymerase I transcription machinery switches from initiation to elongation mode.

    abstract::The amounts of RNA polymerase I (Pol I) and basal rDNA transcription factors were determined in yeast whole cell extracts. A 17-fold excess of Pol I was found compared to the Pol I-specific initiation factors upstream activating factor (UAF) and core factor (CF) which underlines that both initiation factors interact w...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Bier M,Fath S,Tschochner H

    更新日期:2004-04-23 00:00:00

  • Signal peptide homology between the sweet protein thaumatin II and unrelated cereal alpha-amylase/trypsin inhibitors.

    abstract::A cDNA clone (pUP-23) corresponding to a member of a protein family that includes inhibitors of trypsin and of heterologous alpha-amylases has been selected from a library derived from developing barley endosperm and its sequence has been determined. A stretch of 95 nucleotides that included the signal peptide and the...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Lázaro A,Rodriguez-Palenzuela P,Maraña C,Carbonero P,Garcia-Olmedo F

    更新日期:1988-10-24 00:00:00

  • Phosphorylation of the glycogen-binding subunit of protein phosphatase-1G in response to adrenalin.

    abstract::The glycogen-binding (G) subunit of protein phosphatase-1 is phosphorylated in vivo. In rabbits injected with propranolol the serine residue termed site-1 was phosphorylated in 56% of the molecules isolated, and phosphorylation increased to 82% after administration of adrenalin. It is concluded that the G-subunit is a...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: MacKintosh C,Campbell DG,Hiraga A,Cohen P

    更新日期:1988-07-04 00:00:00

  • Microheterogeneity of parsley plastocyanin.

    abstract::A procedure for isolation of two iso-plastocyanins from parsley has been described here. Three consecutive chromatographic steps on DE-52-Whatman cellulose were applied for isolation of two total plastocyanin (PC) fractions, oxidized [PC(II)] and reduced [PC(I)]. By chromatofocusing of PC(II) on Polybuffer exchanger 7...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Dimitrov MI,Donchev AA,Egorov TA

    更新日期:1990-06-04 00:00:00

  • Chemical synthesis and biological activity of a novel antibacterial peptide deduced from a pig myeloid cDNA.

    abstract::Several myeloid precursors of antibacterial peptides have recently been shown to share homologous pre- and pro-regions. Taking advantage of this homology, a novel cDNA was cloned from pig bone marrow RNA. This encodes a 166-residue polypeptide with highly conserved pre- (29 residues) and pro- (101 residues) sequences,...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Storici P,Scocchi M,Tossi A,Gennaro R,Zanetti M

    更新日期:1994-01-17 00:00:00

  • Cell cycle-dependent association of Gag-Mil and hsp90.

    abstract::Immunoprecipitated p100Gag-Mil protein kinase from MH2-transformed quail embryo fibroblasts is associated with an 89 kDa protein. The molar ratio between p89 and Gag-Mil in the immunocomplex is 0.72, indicating that the majority of Gag-Mil is complexed with p89. During mitosis part of Gag-Mil is shifted to a form with...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Lovrić J,Bischof O,Moelling K

    更新日期:1994-04-18 00:00:00

  • 31P NMR as a tool for monitoring detergent solubilization of sarcoplasmic reticulum membranes.

    abstract::Sarcoplasmic reticulum vesicles were solubilized stepwise by the nonionic detergent octaethyleneglycol monododecyl ether; 31P NMR enabled the extent of phospholipid solubilization to be monitored by following the conversion of the broad resonance peak characterizing the phospholipids inserted in the bilayer to the nar...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Roux M,Champeil P

    更新日期:1984-06-11 00:00:00

  • Identification of a unique splicing regulatory cluster in hepatitis B virus pregenomic RNA.

    abstract::HBV particles with genome derived from spliced mRNAs accumulate in patients with virus-derived severe liver necrosis and fibrosis. We investigated the role of an intronic element (intronic splicing silencer-long, ISS(L)) on splicing of HBV minigene transcripts. Removal of the entire ISS(L) showed two-fold increase in ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Chowdhury JB,Roy D,Ghosh S

    更新日期:2011-10-20 00:00:00

  • pH-dependent changes in structure and RNA-binding activity of casein kinase 2 from Rana temporaria oocytes.

    abstract::It is demonstrated by filter-binding assay that casein kinase 2 from Rana temporaria oocytes binds rRNA in vitro with high affinity. Ligand-blotting shows that rRNA-binding activity is inherent to alpha and alpha' subunits of the enzyme. Increase of pH from 6.5 to 7.5 has little effect on casein kinase but completely ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Kandror KV,Kapkov DV,Turapov OA,Stepanov AS

    更新日期:1991-06-03 00:00:00

  • Cloning and DNA sequence of the genes for two bacteriophage T5 tRNAsSer.

    abstract::Bacteriophage T5 Bg/II/Hind III DNA fragment (803 basepairs), containing the genes for 2 tRNAs and 2 RNAs with unknown functions, was cloned in the plasmid pBR322. The analysis of DNA sequence indicates that tRNA genes code isoacceptor tRNAsSer (tRNA1Ser and tRNA2Ser) with anticodons UGA and GGA, respectively. The mai...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Kryukov VM,Ksenzenko VN,Kaliman AV,Bayev AA

    更新日期:1983-07-11 00:00:00

  • Selective inhibition of divergent seryl-tRNA synthetases by serine analogues.

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    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Ahel D,Slade D,Mocibob M,Söll D,Weygand-Durasevic I

    更新日期:2005-08-15 00:00:00

  • A critical appraisal of the mitochondrial coenzyme Q pool.

    abstract::The function of the coenzyme Q (CoQ) pool in the inner mitochondrial membrane is reviewed in view of recent findings suggesting a supramolecular organization of the mitochondrial respiratory complexes. In spite of the structural evidence for preferential aggregations of the inner membrane components, most kinetic evid...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审


    authors: Lenaz G

    更新日期:2001-12-07 00:00:00

  • Oligomeric complexes involved in translocation of proteins across the membrane of the endoplasmic reticulum.

    abstract::Proteins involved in protein translocation across the membrane of the endoplasmic reticulum assemble into different oligomeric complexes depending on their state of function. To analyse such membrane protein complexes we fractionated proteins of mammalian rough microsomes and analysed them using blue native PAGE and i...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Wang L,Dobberstein B

    更新日期:1999-09-03 00:00:00

  • Gcm1, a mammalian homolog of Drosophila glial cells missing.

    abstract::Differentiation of glia (astrocytes and oligodendrocytes) in Drosophila requires the gene glial cells missing (gcm), which controls lineage determination. In the absence of gcm, neuroglia progenitors exclusively differentiate into neurons, instead of into both neurons and glia. In contrast, ectopic overexpression of g...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Altshuller Y,Copeland NG,Gilbert DJ,Jenkins NA,Frohman MA

    更新日期:1996-09-16 00:00:00

  • The gene encoding glutathione-dependent formaldehyde dehydrogenase/GSNO reductase is responsive to wounding, jasmonic acid and salicylic acid.

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    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Díaz M,Achkor H,Titarenko E,Martínez MC

    更新日期:2003-05-22 00:00:00

  • Biochemical identification of the catalytic residues of a glycoside hydrolase family 120 β-xylosidase, involved in xylooligosaccharide metabolisation by gut bacteria.

    abstract::The β-xylosidase B from Bifidobacterium adolescentis ATCC15703 belongs to the newly characterized family 120 of glycoside hydrolases. In order to investigate its catalytic mechanism, an extensive kinetic study of the wild-type enzyme and mutants targeting the three highly conserved residues Asp(393), Glu(416) and Glu(...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Cecchini DA,Fauré R,Laville E,Potocki-Veronese G

    更新日期:2015-10-07 00:00:00

  • Imaging the assembly and disassembly kinetics of cis-SNARE complexes on native plasma membranes.

    abstract::Mild sonication of eukaryotic cells produces native plasma membrane sheets that retain their docked organelles, cytoskeleton structures and cytoplasmic complexes. While the delicate organization of membranous protein complexes remains undisturbed, their inner plasmalemmel leaflet can be rapidly exposed to bathing solu...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Bar-On D,Winter U,Nachliel E,Gutman M,Fasshauer D,Lang T,Ashery U

    更新日期:2008-10-15 00:00:00

  • Prediction of a novel topology in the N-terminal, 14 kDa fragment of Ada protein.

    abstract::Previously determined protein structures have been analysed, in order to find folding motifs similar to that proposed by NMR spectroscopy, for the N-terminal, 14 kDa fragment of the Ada protein. The analyses reveal only limited similarities with the NMR-derived structural data and strongly suggest that this region of ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Swindells MB

    更新日期:1993-06-01 00:00:00

  • Mitogen-inducible gene 6 (MIG-6), adipophilin and tuftelin are inducible by hypoxia.

    abstract::Adaptation to hypoxia is essential for tumor progression. Transcriptional activation of hypoxia-regulated genes is mediated by hypoxia-inducible factor 1 (HIF-1), a heterodimer of HIF-1alpha and ARNT (Ah receptor nuclear translocator; HIF-1beta). Using representational difference analysis, we identified three novel hy...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Saarikoski ST,Rivera SP,Hankinson O

    更新日期:2002-10-23 00:00:00

  • Complete amino acid sequence of human intestinal aminopeptidase N as deduced from cloned cDNA.

    abstract::The complete primary structure (967 amino acids) of an intestinal human aminopeptidase N (EC was deduced from the sequence of a cDNA clone. Aminopeptidase N is anchored to the microvillar membrane via an uncleaved signal for membrane insertion. A domain constituting amino acid 250-555 positioned within the c...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Olsen J,Cowell GM,Kønigshøfer E,Danielsen EM,Møller J,Laustsen L,Hansen OC,Welinder KG,Engberg J,Hunziker W

    更新日期:1988-10-10 00:00:00

  • Connexins in lymphatic vessel physiology and disease.

    abstract::Connexins are transmembrane proteins that form gap junction- and hemi-channels. Once inserted into the membrane, hemi-channels (connexons) allow for diffusion of ions and small molecules (<1 kDa) between the extracellular space and the cytosol. Gap junction channels allow diffusion of similar molecules between the cyt...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审


    authors: Meens MJ,Sabine A,Petrova TV,Kwak BR

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  • IRF-8/ICSBP and IRF-1 cooperatively stimulate mouse IL-12 promoter activity in macrophages.

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    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Masumi A,Tamaoki S,Wang IM,Ozato K,Komuro K

    更新日期:2002-11-06 00:00:00

  • An amino acid substitution that blocks the deacylation step in the enzyme mechanism of penicillin-binding protein 5 of Escherichia coli.

    abstract::A mutant of Escherichia coli has been described that produces an altered form of penicillin-binding protein 5 which still binds penicillin but is unable to catalyse the release of the bound penicilloyl moiety. We show that the mutation is caused by a single nucleotide transition that results in a change from glycine a...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Broome-Smith J,Spratt BG

    更新日期:1984-01-09 00:00:00

  • Analysis of the black-eyed pea trypsin and chymotrypsin inhibitor-alpha-chymotrypsin complex.

    abstract::The black-eyed pea trypsin and chymotrypsin inhibitor (BTCI) is a member of the Bowman-Birk protease inhibitor (BBI) family. The three-dimensional model of the BTCI-chymotrypsin complex was built based on the homology to Bowman-Birk inhibitors with known structures. An extensive theoretical and experimental study of t...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: de Freitas SM,de Mello LV,da Silva MC,Vriend G,Neshich G,Ventura MM

    更新日期:1997-06-09 00:00:00

  • Double-negative feedback loop between long non-coding RNA TUG1 and miR-145 promotes epithelial to mesenchymal transition and radioresistance in human bladder cancer cells.

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    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Tan J,Qiu K,Li M,Liang Y

    更新日期:2015-10-07 00:00:00

  • Lipopolysaccharide signaling induces serum amyloid A (SAA) synthesis in human hepatocytes in vitro.

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    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Migita K,Abiru S,Nakamura M,Komori A,Yoshida Y,Yokoyama T,Daikoku M,Ueki T,Takii Y,Yano K,Yastuhashi H,Eguchi K,Ishibashi H

    更新日期:2004-07-02 00:00:00

  • Phosphorylation and changes in the distribution of nucleolin promote tumor metastasis via the PI3K/Akt pathway in colorectal carcinoma.

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    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Wu DM,Zhang P,Liu RY,Sang YX,Zhou C,Xu GC,Yang JL,Tong AP,Wang CT

    更新日期:2014-05-21 00:00:00

  • His507 of acylaminoacyl peptidase stabilizes the active site conformation, not the catalytic intermediate.

    abstract::Acylaminoacyl peptidase is a member of the prolyl oligopeptidase family. Amino acid sequence alignment suggests that the stabilization of the tetrahedral intermediate should be mediated by His507 rather than by a tyrosine residue found in the other family members of this serine peptidase group. The pH dependence of k(...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Kiss AL,Szeltner Z,Fülöp V,Polgár L

    更新日期:2004-07-30 00:00:00

  • Binding sites in fibronectin for an enterotoxigenic strain of E. coli B342289c.

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    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Visai L,Bozzini S,Petersen TE,Speciale L,Speziale P

    更新日期:1991-09-23 00:00:00