The cytostatic activity of 5-(1-azidovinyl)-2'-deoxyuridine (AzVDU) against herpes simplex virus thymidine kinase gene-transfected FM3A cells is due to inhibition of thymidylate synthase and enhanced by UV light (lambda = 254 nm) exposure.

Abstract:

:5-(1-Azidovinyl)-2'-deoxyuridine (AzVDU) and a series of 5-[1-azido-2-halogenoethyl]-derivatives of beta-D-arabinofuranosyluracil (AU) proved markedly inhibitory to the replication of herpes simplex virus type 1 (HSV-1) and varicella zoster virus (VZV), but not thymidine kinase (TK)-deficient HSV-1 and VZV strains. None of the compounds were cytostatic. However, AzVDU, but not the 5-[1-azido-2-halogenoethyl]-AU derivatives became highly cytostatic against HSV-1 and HSV-2 TK gene-transfected FM3A tumor cells. The molecular target for the cytostatic effect of AzVDU proved to be thymidylate synthase. Short exposure of AzVDU-treated FM3A TK-/HSV-1 TK+ cells to irradiation at lambda = 254 nm enhanced the cytostatic activity of AzVDU by 5-fold.

journal_name

FEBS Lett

journal_title

FEBS letters

authors

Balzarini J,Andrei G,Kumar R,Knaus EE,Wiebe LI,De Clercq E

doi

10.1016/0014-5793(95)00994-k

subject

Has Abstract

pub_date

1995-10-02 00:00:00

pages

41-4

issue

1

eissn

0014-5793

issn

1873-3468

pii

0014-5793(95)00994-K

journal_volume

373

pub_type

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