Glycogen metabolism in a Saccharomyces cerevisiae phosphoglucose isomerase (pgil) disruption mutant.

abstract：:The polyketide synthase associated with the biosynthesis of enediyne-containing calicheamicin contains a putative phosphopantetheinyl transferase (PPTase) domain. By cloning and expressing the C-terminal region of the polyketide synthase and in vitro phosphopantetheinylation assay, we found that the PPTase domain exhi...

journal_title：FEBS letters

authors： Murugan E,Liang ZX

更新日期：2008-04-02 00:00:00

abstract：:VP64 is widely used as a transcriptional activator to investigate the biological function of genes, but its potential for application in genetic improvement of crops has not been fully investigated. Here, we characterized an OsMYB1R1-VP64 fusion protein that enhanced the grain yield of rice cultivar 'Kita-ake' by 35%....

journal_title：FEBS letters

authors： Wang J,Wu F,Zhu S,Xu Y,Cheng Z,Wang J,Li C,Sheng P,Zhang H,Cai M,Guo X,Zhang X,Wang C,Wan J

更新日期：2016-10-01 00:00:00

abstract：:F0F1 ATP synthases are the smallest rotary motors in nature and work as ATP factories in bacteria, plants and animals. Here we report on the first observation of intersubunit rotation in fully coupled single F0F1 molecules during ATP synthesis or hydrolysis. We investigate the Na+-translocating ATP synthase of Propion...

journal_title：FEBS letters

authors： Kaim G,Prummer M,Sick B,Zumofen G,Renn A,Wild UP,Dimroth P

更新日期：2002-08-14 00:00:00

abstract：:Here we report that splice blocking morpholinos (Sb MO) against zebrafish sox31 elicit developmental arrest, likely through creating a series of dominant negative splicing variants. Embryos injected with the Sb MO develop normally before the Mid-Blastula Transition (MBT); however, they do not initiate epiboly. Microar...

journal_title：FEBS letters

authors： Hu SN,Yu H,Zhang YB,Wu ZL,Yan YC,Li YX,Li YY,Li YP

更新日期：2012-02-03 00:00:00

abstract：:We have compared the urea stability of the human aromatic amino acid hydroxylases (AAAHs), key enzymes involved in neurotransmitter biosynthesis and amino acid homeostasis. Tyrosine-, tryptophan- and phenylalanine hydroxylase (TH, TPH and PAH, respectively) were transiently activated at low urea concentrations and rap...

journal_title：FEBS letters

authors： Kleppe R,Haavik J

更新日期：2004-05-07 00:00:00

abstract：:Little is known about the mechanisms underlying calcium-induced Ca2+ release (CICR) in plants. The slow-activating vacuolar (SV) channel is both permeable to, and activated by Ca2+, and is therefore a prime candidate for a role in CICR. Cytosol-side-out vacuolar membrane vesicles loaded with 45Ca2+ showed voltage- and...

journal_title：FEBS letters

authors： Bewell MA,Maathuis FJ,Allen GJ,Sanders D

更新日期：1999-09-10 00:00:00

abstract：:Irreversible conversion of the purified zinc-containing 7Fe ferredoxin from the thermoacidophilic archaeon Sulfolobus sp. strain 7 was found to occur under aerobic conditions at pH 5.0 and at 4 degrees C. This process accompanied a substantial increase of the electron paramagnetic resonance signal attributed to a [3Fe...

journal_title：FEBS letters

authors： Iwasaki T,Oshima T

更新日期：1997-11-10 00:00:00

abstract：:Dimethylsulphoxide (DMSO) reductase from R. capsulatus contains a molybdenum-pterin cofactor at its active site. As prepared the molybdenum is in the 6+ oxidation state, devoid of EPR signals. Stepwise reduction generates an EPR signal characteristic of Mo(V) having hyperfine coupling to a single proton and integratin...

journal_title：FEBS letters

authors： Benson N,Farrar JA,McEwan AG,Thomson AJ

更新日期：1992-07-28 00:00:00

abstract：:The amino acid residue Asn141 of the restriction endonuclease EcoRI was proposed to make three hydrogen bonds to both adenine residues within the recognition sequence -GAATTC-. We have mutated Asn141 to alanine, aspartate, serine, and tyrosine. Only the serine mutant is active under normal buffer conditions although 1...

journal_title：FEBS letters

authors： Fritz A,Küster W,Alves J

更新日期：1998-10-30 00:00:00

abstract：:VASP (vasodilator-stimulated phosphoprotein), a protein associated with microfilaments at cellular contact sites, has been identified as a ligand for profilin and zyxin, two proteins also involved in microfilament dynamics and organization at these regions. Here, we report that VASP also directly binds to vinculin, an...

journal_title：FEBS letters

authors： Reinhard M,Rüdiger M,Jockusch BM,Walter U

更新日期：1996-12-09 00:00:00

abstract：:The cDNA coding for a novel member of the cysteine-rich protein family was isolated from a rat brain cDNA library. It encodes a protein, denoted cysteine-rich protein 2 (CRP2), of 208 amino acid residues containing two tandem repeats of an unusual LIM/double zinc-finger-like motif. The ubiquitous tissue distribution a...

journal_title：FEBS letters

authors： Okano I,Yamamoto T,Kaji A,Kimura T,Mizuno K,Nakamura T

更新日期：1993-10-25 00:00:00

abstract：:The dinuclear paramagnetic center of the soluble CuA domain of the cytochrome c oxidase from Bacillus subtilis has been studied using 1H NMR. The spectrum possesses remarkably sharp shifted resonances. Comparison with the spectrum of the CuA amicyanin variant provides the spin density distribution in the CuA site of c...

journal_title：FEBS letters

authors： Dennison C,Berg A,de Vries S,Canters GW

更新日期：1996-10-07 00:00:00

abstract：:Secreted proteins are indispensable for the development and differentiation of multicellular organisms. Cloning and characterization of novel or hypothetical genes encoding these proteins are therefore inviting great incentives. Using bioinformatics tools and experimental approaches, we isolated and characterized a hu...

journal_title：FEBS letters

authors： Zeng LC,Liu F,Zhang X,Zhu ZD,Wang ZQ,Han ZG,Ma WJ

更新日期：2004-07-30 00:00:00

abstract：:In lower and higher eukaryotes, a family of tightly related proteins designated eRF1 (for eukaryotic release factor 1) catalyses termination of protein synthesis at all three stop codons. The human genome contains four eRF1 homologous sequences localised on chromosomes 5, 6, 7 and X. We report here the cloning and the...

journal_title：FEBS letters

authors： Guenet L,Toutain B,Guilleret I,Chauvel B,Deaven LL,Longmire JL,Le Gall JY,David V,Le Treut A

更新日期：1999-07-02 00:00:00

abstract：:α-Glucosidase hydrolyzes α-glucosides and transfers α-glucosyl residues to an acceptor through transglucosylation. In this study, GH13_31 α-glucosidase BspAG13_31A with high transglucosylation activity is reported in Bacillus sp. AHU2216 and biochemically and structurally characterized. This enzyme is specific to α-(1...

journal_title：FEBS letters

authors： Auiewiriyanukul W,Saburi W,Kato K,Yao M,Mori H

更新日期：2018-07-01 00:00:00

abstract：:The aim of this study was to evaluate the involvement of oxidative stress in glutamate-evoked transglutaminase (TGase) upregulation in astrocyte cultures (14 DIV). A 24 h exposure to glutamate caused a dose-dependent depletion of glutathione intracellular content and increased the ROS production in cell cultures. Thes...

journal_title：FEBS letters

authors： Campisi A,Caccamo D,Li Volti G,Currò M,Parisi G,Avola R,Vanella A,Ientile R

更新日期：2004-12-03 00:00:00

abstract：:Cytochrome c oxidase has been isolated from beef heart mitochondria and labeled with the fluorochrome eosin-5-maleimide (EMA) after pretreatment with mersalyl. On SDS-polyacrylamide gels, EMA fluorescence and absorption occurred at a single band corresponding to subunit III. Since only Cys 115 of the two cysteinyl res...

journal_title：FEBS letters

authors： Müller M,Azzi A

更新日期：1985-05-06 00:00:00

abstract：:A central step in xanthone biosynthesis is the formation of the C13 skeleton, i.e. an intermediate benzophenone. Biosynthesis of 2,3',4,6-tetrahydroxybenzophenone from m-hydroxybenzoyl-CoA and malonyl-CoA was shown in cell-free extracts from cultured cells of Centaurium erythraea. The enzyme catalyzing this reaction w...

journal_title：FEBS letters

authors： Beerhues L

更新日期：1996-04-01 00:00:00

abstract：:The roles of 2'-OH groups in the binding of mRNA to human ribosomes were studied using site-directed cross-linking. We found that both mRNA and mDNA analogues bearing a cross-linker can modify ribosomal proteins (rps) S3e and S2e at the mRNA entry site independently on tRNA presence, but only mRNA analogues were capab...

journal_title：FEBS letters

authors： Graifer D,Zhigailov A,Ven'yaminova A,Malygin A,Iskakov B,Karpova G

更新日期：2012-10-19 00:00:00

abstract：:The fat mouse strain exhibits a late-onset obesity syndrome associated with a mutation in the gene encoding carboxypeptidase E (CPE). Since CPE plays a central role in the biosynthesis of a number of regulatory peptides, including gastrin, we examined the biogenesis and processing of progastrin in fat/fat mice by meas...

journal_title：FEBS letters

authors： Lacourse KA,Friis-Hansen L,Rehfeld JF,Samuelson LC

更新日期：1997-10-13 00:00:00

abstract：:We report that sodium butyrate, a natural product of fiber degradation by colonic bacteria, markedly suppresses c-Myc-mediated apoptosis induced in murine plasmacytomas and human Burkitt lymphomas by growth factor deprivation, but not in cell lines devoid of c-myc translocations. Attenuation of cell death is associate...

journal_title：FEBS letters

authors： Alexandrov I,Romanova L,Mushinski F,Nordan R

更新日期：1998-08-28 00:00:00

abstract：:A number of recent observations have suggested that the endocytic and biosynthetic pathways may share fundamentally similar transport mechanisms at the molecular level. Some of the more striking of these suggestions have come from a comparison of the effects of the macrocyclic lactone brefeldin A (BFA) on endosomes an...

journal_title：FEBS letters

authors： Hunziker W,Whitney JA,Mellman I

更新日期：1992-07-27 00:00:00

abstract：:The AMP-activated protein kinase (AMPK) is the central component of a protein kinase cascade that plays a major role in energy sensing. AMPK is activated pharmacologically by 5-amino-4-imidazolecarboxamide (AICA) riboside monophosphate (ZMP), which mimics the effects of AMP on the AMPK cascade. Here we show that uptak...

journal_title：FEBS letters

authors： Fryer LG,Parbu-Patel A,Carling D

更新日期：2002-11-06 00:00:00

abstract：:A high intake of dietary fat has been suggested to diminish mitochondrial functioning in skeletal muscle, possibly attributing to muscular fat accumulation. Here we show however, that an 8-week high-fat dietary intervention did not affect intrinsic functioning of rat skeletal muscle mitochondria assessed by respiromet...

journal_title：FEBS letters

authors： Hoeks J,Briedé JJ,de Vogel J,Schaart G,Nabben M,Moonen-Kornips E,Hesselink MK,Schrauwen P

更新日期：2008-02-20 00:00:00

abstract：:In order to elucidate the molecular mechanisms responsible for the specific lipid-protein interactions, we have undertaken structural and idiotypic analyses of a monoclonal antibody, PS4A7, which binds specifically to phosphatidylserine (PS). Here we showed that one of the anti-idiotypic monoclonal antibodies raised a...

journal_title：FEBS letters

authors： Reza F,Igarashi K,Tokita S,Asai K,Aoki J,Asaoka Y,Umeda M,Inoue K

更新日期：1994-02-21 00:00:00

abstract：:One key area of protein kinase research is the identification of cognate substrates. The search for substrates is hampered by problems in unambiguously assigning substrates to a particular kinase in vitro and in vivo. One solution to this impasse is to engineer the kinase of interest to accept an ATP analogue which is...

journal_title：FEBS letters

authors： Hindley AD,Park S,Wang L,Shah K,Wang Y,Hu X,Shokat KM,Kolch W,Sedivy JM,Yeung KC

更新日期：2004-01-02 00:00:00

abstract：:The Hippo-Warts pathway defines a novel signaling cascade involved in organ size control and tumor suppression. However, the developmental function of this pathway is less understood. Here we report that the Caenorhabditis elegans homolog of Warts, Ce-wts-1, plays important roles during worm development. The null alle...

journal_title：FEBS letters

authors： Cai Q,Wang W,Gao Y,Yang Y,Zhu Z,Fan Q

更新日期：2009-10-06 00:00:00

abstract：:In this study, we have identified 876 polymorphism sites in 145 complete or partial genomes of SARS-CoV available in the NCBI GenBank. One hundred and seventy-four of these sites existed in two or more SARS-CoV genome sequences. According to the sequence polymorphism, all SARS-CoVs can be divided into three groups: (I...

journal_title：FEBS letters

authors： Wang ZG,Zheng ZH,Shang L,Li LJ,Cong LM,Feng MG,Luo Y,Cheng SY,Zhang YJ,Ru MG,Wang ZX,Bao QY

更新日期：2005-09-12 00:00:00

abstract：:Hematopoietic progenitors have been shown to retain plasticity and switch lineages by appropriate stimuli. However, mature blood cells hardly showed such differentiation plasticity. In this paper, we tried to reprogram mature B cells into erythroid lineage by expressing various hematopoietic transcription factors. Amo...

journal_title：FEBS letters

authors： Sadahira K,Fukuchi Y,Kunimono H,Sakurai M,Ikeda Y,Okamoto S,Nakajima H

更新日期：2012-10-19 00:00:00

abstract：:The Xenopus laevis oocyte has endogenous sites that bind muscarinic agonists, which have been pharmacologically characterized as M3 and/or M1 receptor subtypes. In order to define the molecular identify of the receptor protein we have analyzed a Xenopus oocyte cDNA library and cloned a 2.9 kb cDNA fragment encoding a ...

journal_title：FEBS letters

authors： Herrera L,Carvallo P,Antonelli M,Olate J

更新日期：1994-09-26 00:00:00